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pGTag-P2A-QFGal4-SV40pA

Plasmid

#155127

Purpose

Donor for precise CRISPR directed genomic integration of QFGal4 using the GeneWeld method

Depositor

Bret Pearson

Article

Burgess et al Dev Biol. 2020 Jul 19. pii: S0012-1606(2

Insert

P2A-QFGal4

Use

CRISPR and Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Sept. 14, 2020

Availability

Academic Institutions and Nonprofits only

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pSin-5HT-1.0

Plasmid

#172882

Purpose

make sindbis virus of 5-HT sensor GRAB_5-HT1.0 and express in cells

Depositor

J. Julius Zhu

Article

Wan et al Nat Neurosci. 2021 Apr 5. pii: 10.1038/s

Insert

5HT SENSOR

Use

Tags

Expression

Mammalian

Mutation

Promoter

sp6

Available since

Oct. 3, 2021

Availability

Academic Institutions and Nonprofits only

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pBluescriptII[attB/Ins1]

Plasmid

#74100

Purpose

"intermediate vector" for the two-step cloning method (Wang and Szaro, Dev Biol, 2015)

Depositor

Ben Szaro

Article

Wang et al Dev Biol. 2015 Feb 1;398(1):11-23. doi:

Inserts

Phi C31 attb element
Dual chicken beta-globin hypersensitive site 4 (HS4) insulators

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

May 26, 2016

Availability

Academic Institutions and Nonprofits only

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645 pCS2-etv2

Plasmid

#49005

Purpose

Mammalian Expression vector, In-situ probe template and in vitro transcription vector for zebrafish Etv2

Depositor

Nathan Lawson

Article

Moore et al Dev Biol. 2013 Sep 11. pii: S0012-1606(1

Insert

ets variant gene 2 (etsrp Zebrafish)

Use

Invitro transcription, riboprobe template

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 28, 2013

Availability

Academic Institutions and Nonprofits only

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pSPORT1[NheI/glGFP/bglobin3'-UTR/Ins2]

Plasmid

#74101

Purpose

vector bearing an NheI restriction site for the two-step cloning method in Wang and Szaro, Dev Biol, 2015

Depositor

Ben Szaro

Article

Wang et al Dev Biol. 2015 Feb 1;398(1):11-23. doi:

Inserts

Green Lantern Green Fluorescent Protein
Rabbit beta 1-globin gene 3'UTR

Use

Tags

Expression

Mutation

Promoter

Available since

May 26, 2016

Availability

Academic Institutions and Nonprofits only

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pOEM-GED-EGFP

Plasmid

#102782

Purpose

Baculovirus rescue vector, VSVGED pseudotype, eGFP

Depositor

Elly Tanaka

Article

Oliveira et al Dev Biol. 2018 Jan 15;433(2):262-275. do

Insert

EGFP

Use

Baculoviral rescue shuttle vector

Tags

Expression

Insect and Mammalian

Mutation

Promoter

CMV

Available since

Dec. 21, 2017

Availability

Academic Institutions and Nonprofits only

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pBS DKK3

Plasmid

#19201

Depositor

Cliff Tabin

Article

Bandyopadhyay et al Dev Biol. 2008 Sep 1;321(1):162-74. doi:

Insert

Dickkopf homolog 3 (DKK3 Chicken)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 19, 2008

Availability

Academic Institutions and Nonprofits only
459 pME-etv2

Plasmid

#49002

Purpose

full length coding sequence for zebrafish etv2 gene in gateway middle entry vector

Depositor

Nathan Lawson

Article

Moore et al Dev Biol. 2013 Sep 11. pii: S0012-1606(1

Insert

ets variant gene 2 (etsrp Zebrafish)

Use

Gateway middle entry

Tags

Expression

Mutation

Promoter

Available since

Oct. 28, 2013

Availability

Academic Institutions and Nonprofits only

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pOEM-GED-mCherry

Plasmid

#102781

Purpose

Baculovirus rescue vector, VSVGED pseudotype, mCherry

Depositor

Elly Tanaka

Article

Oliveira et al Dev Biol. 2018 Jan 15;433(2):262-275. do

Insert

mCherry

Use

Baculoviral rescue shuttle vector

Tags

Expression

Insect and Mammalian

Mutation

Promoter

CMV

Available since

Dec. 21, 2017

Availability

Academic Institutions and Nonprofits only

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pBS MAFB

Plasmid

#19211

Depositor

Cliff Tabin

Article

Bandyopadhyay et al Dev Biol. 2008 Sep 1;321(1):162-74. doi:

Insert

MAFB (MAFB Chicken)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 19, 2008

Availability

Academic Institutions and Nonprofits only
592 p3e-EST etv2 3’UTR

Plasmid

#49003

Purpose

Zebrafish EST annotated length etv2 3' UTR in gateway 3' Entry vector

Depositor

Nathan Lawson

Article

Moore et al Dev Biol. 2013 Sep 11. pii: S0012-1606(1

Insert

ets variant gene 2 (etsrp Zebrafish)

Use

Gateway 3' entry

Tags

Expression

Mutation

only the 3' UTR

Promoter

3' gateway entry vector

Available since

Oct. 28, 2013

Availability

Academic Institutions and Nonprofits only

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1208 p3e-long etv2 3’UTR

Plasmid

#49010

Purpose

Zebrafish Long length etv2 3' UTR in gateway 3' Entry vector

Depositor

Nathan Lawson

Article

Moore et al Dev Biol. 2013 Sep 11. pii: S0012-1606(1

Insert

ets variant gene 2 (etsrp Zebrafish)

Use

Gateway 3' entry

Tags

Expression

Mutation

Longest 3' UTR

Promoter

Available since

Oct. 28, 2013

Availability

Academic Institutions and Nonprofits only

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pBS Calnexin

Plasmid

#19208

Depositor

Cliff Tabin

Article

Bandyopadhyay et al Dev Biol. 2008 Sep 1;321(1):162-74. doi:

Insert

Calnexin (CANX Chicken)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 19, 2008

Availability

Academic Institutions and Nonprofits only
pBS BRD7

Plasmid

#19232

Depositor

Cliff Tabin

Article

Bandyopadhyay et al Dev Biol. 2008 Sep 1;321(1):162-74. doi:

Insert

Bromodomain containing 7 (BRD7 Chicken)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 8, 2008

Availability

Academic Institutions and Nonprofits only
pBS TMEM108

Plasmid

#18852

Depositor

Cliff Tabin

Article

Bandyopadhyay et al Dev Biol. 2008 Sep 1;321(1):162-74. doi:

Insert

Hypothetical protein, Tmem108 (HAPLN1 Chicken)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 15, 2008

Availability

Academic Institutions and Nonprofits only
pBS C1QTNF3

Plasmid

#19221

Depositor

Cliff Tabin

Article

Bandyopadhyay et al Dev Biol. 2008 Sep 1;321(1):162-74. doi:

Insert

Collagenous repeat-containing (C1QTNF3 Chicken)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 19, 2008

Availability

Academic Institutions and Nonprofits only
pBS FAM20C

Plasmid

#19214

Depositor

Cliff Tabin

Article

Bandyopadhyay et al Dev Biol. 2008 Sep 1;321(1):162-74. doi:

Insert

FAM20C (FAM20C Chicken)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 19, 2008

Availability

Academic Institutions and Nonprofits only
pBS Calponin3

Plasmid

#19194

Depositor

Cliff Tabin

Article

Bandyopadhyay et al Dev Biol. 2008 Sep 1;321(1):162-74. doi:

Insert

Calponin 3 (CNN3 Chicken)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 19, 2008

Availability

Academic Institutions and Nonprofits only
pCAG-3xGFP11-mem-3xControlgRNA

Plasmid

#224583

Purpose

Ubiquitous expression plasmid with CAG promoter (CMV immediate early enhancer, chicken beta actin promoter), three Control gRNAs with ribozyme self-cleavage, three membrane split GFP(11) reporter.

Depositor

Erica Hutchins

Article

Kim et al Dev Biol. 2024 Nov 30:S0012-1606(24)0026

Insert

3xGFP11

Use

CRISPR

Tags

Membrane Localization Signal

Expression

Mutation

Codon optimized

Promoter

Available since

Dec. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pEM.H10L

Plasmid

#198683

Purpose

An Easy Modular Integrative fuSion-ready Expression toolkit (Easy-MISE toolkit) for fast engineering of heterologous productions in Saccharomyces cerevisiae

Depositor

Paola Branduardi

Article

Maestroni et al ACS Synth Biol. 2023 Apr 14. doi: 10.102

Insert

XI1_UP

Use

Synthetic Biology

Tags

Expression

Yeast

Mutation

Promoter

Available since

June 14, 2023

Availability

Academic Institutions and Nonprofits only

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