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Showing: 181 - 200 of 761 results

  4. pU6_(GLuc)_INT[3xPP7_SL]

    Plasmid
    #68424
    Purpose
    Transient expression of an "INT" construct_bearing three PP7 Stem-loops, targeting the GLuc reporter, in mammalian cells. U6 promoter
    Depositor
    John Rinn
    Article
    Shechner et al Nat Methods. 2015 Jul;12(7):664-70. doi:
    Insert
    INT construct bearing three PP7 Stem-loops
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    human U6
    Available since
    Oct. 5, 2015
    Availability
    Academic Institutions and Nonprofits only
    68424-plasmid-map-sequence-id-125555

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  6. pCMV/3' Box_(GLuc)_INT

    Plasmid
    #68436
    Purpose
    Transient expression of an "INT" construct_bearing three PP7 Stem-loops, targeting the GLuc reporter, in mammalian cells. CMV/3' Box expression backbone.
    Depositor
    John Rinn
    Article
    Shechner et al Nat Methods. 2015 Jul;12(7):664-70. doi:
    Insert
    INT construct_bearing three PP7 Stem-loops
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Sept. 22, 2015
    Availability
    Academic Institutions and Nonprofits only
    68436-plasmid-map-sequence-id-120214

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  9. pU1/Sm/3' Box_(GLuc)_INT

    Plasmid
    #68438
    Purpose
    Transient expression of an "INT" construct_bearing three PP7 Stem-loops, targeting the GLuc reporter, in mammalian cells. U1Pro/SmBox/3' Box expression backbone.
    Depositor
    John Rinn
    Article
    Shechner et al Nat Methods. 2015 Jul;12(7):664-70. doi:
    Insert
    INT construct_bearing three PP7 Stem-loops
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    human U1
    Available since
    Oct. 5, 2015
    Availability
    Academic Institutions and Nonprofits only
    68438-plasmid-map-sequence-id-125562

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  10. pU6_(GLuc)_INT[P4-P6(3xPP7)]

    Plasmid
    #68432
    Purpose
    Transient expression in mammalian cells of an "INT" construct_bearing P4_P6 (internally appended with three PP7 stem-loops), targeting the GLuc reporter. U6 promoter
    Depositor
    John Rinn
    Article
    Shechner et al Nat Methods. 2015 Jul;12(7):664-70. doi:
    Insert
    INT construct_bearing P4_P6 (internally appended with three PP7 stem-loops)
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    human U6
    Available since
    Oct. 5, 2015
    Availability
    Academic Institutions and Nonprofits only
    68432-plasmid-map-sequence-id-125560

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  12. pmalc4x_E323K_TEV

    Plasmid
    #47699
    Purpose
    Expresses MBP myocilin olfactomedin domain fusion protein with a TEV protease recognition sequence and the mutation E323K
    Depositor
    Raquel Lieberman
    Article
    Burns et al Biochemistry. 2011 Jul 5;50(26):5824-33.
    Insert
    Maltose Binding Protein and Olfactomedin-like domain with TEV cleavage site (MYOC Human)
    Use
    Tags
    MBP
    Expression
    Bacterial
    Mutation
    changed glutamic acid 323 to lysine
    Promoter
    Ptac
    Available since
    Sept. 9, 2013
    Availability
    Industry, Academic Institutions, and Nonprofits
    47699-plasmid-map-sequence-id-68104

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  15. MT2a_K30A

    Plasmid
    #105694
    Purpose
    Bacterial expression plasmids for production of recombinant metallothionein 2a K30A mutant
    Depositor
    Artur Krężel
    Article
    Singh et al Metallomics. 2023 Jun 1;15(6):mfad029. d
    Insert
    Metallothionein 2a (MT2A Human)
    Use
    Tags
    Chiting Binding Domanin-Intein
    Expression
    Bacterial
    Mutation
    Lysine 30 to Alanine
    Promoter
    Available since
    April 27, 2020
    Availability
    Academic Institutions and Nonprofits only
    105694-plasmid-map-sequence-id-203312

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  16. MT2a_K31A

    Plasmid
    #105695
    Purpose
    Bacterial expression plasmids for production of recombinant metallothionein 2a K31A mutant
    Depositor
    Artur Krężel
    Article
    Singh et al Metallomics. 2023 Jun 1;15(6):mfad029. d
    Insert
    Metallothionein 2a (MT2A Human)
    Use
    Tags
    Chiting Binding Domanin-Intein
    Expression
    Bacterial
    Mutation
    Lysine 31 to Alanine
    Promoter
    Available since
    April 27, 2020
    Availability
    Academic Institutions and Nonprofits only
    105695-plasmid-map-sequence-id-203313

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  17. MT2a_C21A

    Plasmid
    #105697
    Purpose
    Bacterial expression plasmids for production of recombinant metallothionein 2a C21A mutant
    Depositor
    Artur Krężel
    Article
    Singh et al Metallomics. 2023 Jun 1;15(6):mfad029. d
    Insert
    Metallothionein 2a (MT2A Human)
    Use
    Tags
    Chiting Binding Domanin-Intein
    Expression
    Bacterial
    Mutation
    Cysteine 21 to Alanine
    Promoter
    Available since
    April 27, 2020
    Availability
    Academic Institutions and Nonprofits only
    105697-plasmid-map-sequence-id-203314

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  18. MT2a_K20A

    Plasmid
    #105698
    Purpose
    Bacterial expression plasmids for production of recombinant metallothionein 2a K20A mutant
    Depositor
    Artur Krężel
    Article
    Singh et al Metallomics. 2023 Jun 1;15(6):mfad029. d
    Insert
    Metallothionein 2a (MT2A Human)
    Use
    Tags
    Chiting Binding Domanin-Intein
    Expression
    Bacterial
    Mutation
    Lysine 20 to Alanine
    Promoter
    Available since
    April 27, 2020
    Availability
    Academic Institutions and Nonprofits only
    105698-plasmid-map-sequence-id-203315

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  19. MT2a_S6A

    Plasmid
    #105699
    Purpose
    Bacterial expression plasmids for production of recombinant metallothionein 2a S6A mutant
    Depositor
    Artur Krężel
    Article
    Singh et al Metallomics. 2023 Jun 1;15(6):mfad029. d
    Insert
    Metallothionein 2a (MT2A Human)
    Use
    Tags
    Chiting Binding Domanin-Intein
    Expression
    Bacterial
    Mutation
    Serine 6 to Alanine
    Promoter
    Available since
    April 27, 2020
    Availability
    Academic Institutions and Nonprofits only
    105699-plasmid-map-sequence-id-203317

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  20. pJB03

    Plasmid
    #111961
    Purpose
    Expresses MBP-GFP-DHFR; construct generated for pull-down and localization experiments
    Depositor
    Matthew Good
    Article
    Caldwell et al Biochemistry. 2018 May 8;57(18):2590-259
    Inserts
    mEGFP
    eDHFR
    Use
    Tags
    DHFR
    Expression
    Bacterial
    Mutation
    eDHFR;residues 2-159 and enhanced GFP with monome…
    Promoter
    Tac
    Available since
    Aug. 22, 2018
    Availability
    Academic Institutions and Nonprofits only
    111961-plasmid-map-sequence-id-219904

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Showing: 181 - 200 of 761 results