We narrowed to 5,482 results for: pcas

1. pCAT221

   Plasmid

   #134889

   Purpose

   Bacterial expression

   Depositor

   Thomas Howard

   Article

   Azubuike et al N Biotechnol. 2021 Nov 25;65:20-30. doi:

   Insert

   eGFP
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   July 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. HeFm2SpCas9

   Plasmid

   #92111

   Purpose

   Expression plasmid for human codon-optimized increased fidelity HeFm2SpCas9 (without U6-sgRNA coding sequence)

   Depositor

   Ervin Welker

   Article

   Kulcsar et al Genome Biol. 2017 Oct 6;18(1):190. doi:

   Insert

   “Highly enhanced Fidelity” mut2 SpCas9
   

   Use

   CRISPR

   Tags

   3xFLAG and NLS

   Expression

   Mammalian

   Mutation

   N497A, R661A, Q695A, K848A, Q926A, R1060A

   Promoter

   Cbh

   Available since

   Oct. 31, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCAD54_CAD29_H104A_pvp008

   Plasmid

   #124876

   Purpose

   Expresses hCAD His103Ala mutant in E.coli

   Depositor

   Konrad Buessow

   Article

   Chen et al Proc Natl Acad Sci U S A. 2019 Sep 23. p

   Insert

   cis-aconitate decarboxylase (ACOD1 Human)
   

   Use

   Tags

   StrepTag and TEV site

   Expression

   Bacterial

   Mutation

   mutated His103 to Ala, deleted amino acids 1-3 an…

   Promoter

   T7

   Available since

   Sept. 16, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCARSF85

   Plasmid

   #64694

   Purpose

   H6-TF-Smt3-GB1-NpuDnaEN102-CBD

   Depositor

   Hideo Iwai

   Article

   Guerrero et al Protein Expr Purif. 2015 Aug 20. pii: S1

   Type

   Empty backbone

   Use

   Tags

   CBD, HisTag, NpuDnaE-N102, Smt3, and TF

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Sept. 11, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCAGGS_2pabPAC

   Plasmid

   #158968

   Purpose

   2P-activatable bPAC for transient expression

   Depositor

   Michiyuki Matsuda

   Article

   Kinjo et al ACS Chem Biol. 2020 Oct 19. doi: 10.1021

   Insert

   2pabPAC
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Oct. 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCA0.002

   Plasmid

   #119560

   Purpose

   Level 0 acceptor vector for promoters with lacZ compatible with blue/white screening, low copy number, pSC101 ori.

   Depositor

   Alistair McCormick

   Article

   Vasudevan et al Plant Physiol. 2019 May;180(1):39-55. do

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCas2F

   Plasmid

   #82377

   Purpose

   dCas9 expressed from aTc-inducible promoter in acsA, F RBS: TGGGCG

   Depositor

   Brian Pfleger

   Article

   Gordon et al Metab Eng. 2016 Jul 29. pii: S1096-7176(

   Insert

   dCas9
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   PEZ3

   Available since

   Sept. 16, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. B-HeFSpCas9

   Plasmid

   #126766

   Purpose

   Expression plasmid for human codon-opt. increased fidelity Blackjack-HeFSpCas9 (w/o U6-sgRNA). Cleaves both 20nt and 5'-extended 21nt spacer containing sgRNAs with higher fidelity than HeFSpCas9.

   Depositor

   Ervin Welker

   Article

   Kulcsar et al Nat Commun. 2020 Mar 6;11(1):1223. doi:

   Insert

   B-HeFSpCas9
   

   Use

   CRISPR

   Tags

   3xFLAG and NLS

   Expression

   Mammalian

   Mutation

   N497A; R661A; Q695A; K848A; Q926A; K1003A; R1060A…

   Promoter

   CBh

   Available since

   March 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCAM15

   Plasmid

   #86472

   Purpose

   Expresses p12 and p9-mCerulean

   Depositor

   Pamela Silver

   Article

   Myhrvold et al ACS Synth Biol. 2016 Aug 3.

   Insert

   p12, p9-mCerulean
   

   Use

   Synthetic Biology

   Tags

   mCerulean

   Expression

   Bacterial

   Mutation

   Promoter

   pBAD

   Available since

   July 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCAS_gpyrG1

    Plasmid

    #90276

    Purpose

    (also pMST621-BB3_gpyrG1_cas9) CRISPR/Cas9 plasmid with gRNA for pyrG1, Cas9

    Depositor

    Michael Sauer

    Article

    Sarkari et al Bioresour Technol. 2017 May 4. pii: S096

    Insert

    gRNA (pyrg1)
    

    Use

    A. niger

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCarCBA

    Plasmid

    #117754

    Purpose

    Codon optimized CarCBA enzymes from Pectobacterium carotovorum in pBbaA5k backbone

    Depositor

    Greg Bokinsky

    Article

    Shomar et al Nat Chem Biol. 2018 Aug;14(8):794-800. d

    Insert

    Codon optimised Carbapenem synthase (CarC), Carboxymethylproline synthase (CarB), Carbapenam synthetase (CarA)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PLac(UV5)

    Available since

    Nov. 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCAT526

    Plasmid

    #180513

    Purpose

    Plasmid expressing mammalian codon optimized wt PlmCasX, mNeonGreen, sgRNAv1 scaffold, and restriction sites to clone in new spacers

    Depositor

    Jennifer Doudna

    Article

    Tsuchida et al Mol Cell. 2022 Mar 17;82(6):1199-1209.e6

    Inserts

    CasX sgRNAv1
    PlmCasX-2A-mNeonGreen
    

    Use

    CRISPR

    Tags

    2x FLAG and SV40 NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CAG and U6

    Available since

    April 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCAB42

    Plasmid

    #17268

    Depositor

    Stuart Schreiber

    Article

    Huang et al Proc Natl Acad Sci U S A. 1997 Dec 9. 94

    Type

    Empty backbone

    Use

    Tags

    B42•HA

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Jan. 22, 2010

    Availability

    Academic Institutions and Nonprofits only

14. pCas9GG

    Plasmid

    #131009

    Purpose

    Backbone plasmid for generating CRISPR arrays for SpCas9 using CRATES. Contains a direct repeat and a RFP-dropout cassette.

    Depositor

    Chase Beisel

    Article

    Liao et al Nat Commun. 2019 Jul 3;10(1):2948. doi:

    Inserts

    direct repeat of SpCas9
    promoter PJ23119
    mRFP expression cassette
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Bba_R0040 TetR

    Available since

    Sept. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCAM110

    Plasmid

    #86480

    Purpose

    Expresses p12 and p9-6His-tnaA

    Depositor

    Pamela Silver

    Article

    Myhrvold et al ACS Synth Biol. 2016 Aug 3.

    Insert

    p12, p9-6His-tnaA
    

    Use

    Synthetic Biology

    Tags

    6His and tnaA

    Expression

    Bacterial

    Mutation

    Promoter

    pBAD

    Available since

    April 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCAM9

    Plasmid

    #78560

    Purpose

    pBAD30::ompU V. cholerae 395 Induce with 0.2% arabinose. Expression system: V. cholerae 395 outer membrane protein OmpU.

    Depositor

    James Kaper

    Article

    Merrell et al J Bacteriol. 2001 May;183(9):2746-54.

    Insert

    ompU
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    araBAD promoter

    Available since

    June 8, 2016

    Availability

    Academic Institutions and Nonprofits only

17. pCA0.001

    Plasmid

    #119557

    Purpose

    Level 0 acceptor vector for promoters truncated to transcriptional start site with lacZ compatible with blue/white screening

    Depositor

    Alistair McCormick

    Article

    Vasudevan et al Plant Physiol. 2019 May;180(1):39-55. do

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCAM10

    Plasmid

    #86483

    Purpose

    Expresses p12 and p9-mRFP1

    Depositor

    Pamela Silver

    Article

    Myhrvold et al Nat Commun. 2015 Nov 30;6:10039. doi: 10

    Insert

    p12, p9-mRFP1
    

    Use

    Tags

    mRFP1

    Expression

    Bacterial

    Mutation

    Promoter

    pBAD

    Available since

    Nov. 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCarCBAE

    Plasmid

    #117755

    Purpose

    Codon optimized CarCBAE enzymes from Pectobacterium carotovorum in pBbaA5k backbone

    Depositor

    Greg Bokinsky

    Article

    Shomar et al Nat Chem Biol. 2018 Aug;14(8):794-800. d

    Insert

    Codon optimised Carbapenem synthase (CarC), Carboxymethylproline synthase (CarB), Carbapenam synthetase (CarA), Ferredoxin CarE
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PLac(UV5)

    Available since

    Nov. 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCas2D

    Plasmid

    #82375

    Purpose

    dCas9 expressed from aTc-inducible promoter in acsA, D RBS: AGGACG

    Depositor

    Brian Pfleger

    Article

    Gordon et al Metab Eng. 2016 Jul 29. pii: S1096-7176(

    Insert

    dCas9
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    PEZ3

    Available since

    Sept. 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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