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Showing: 3961 - 4000 of 8321 results

  1. pOliSpec5'

    Plasmid
    #228551
    Purpose
    Template for PCR amplification for gRNA cloning. The PCR product recombines in vivo with a PCRs products from pOliSpec3' and oligo-spacer amplifications, to clone gRNA plasmid
    Depositor
    Jeferson Gross
    Insert
    Truncated 5' region of aadA
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 2, 2024
    Availability
    Academic Institutions and Nonprofits only
    228551-plasmid-map-sequence-id-454860

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  2. pOliSpec3'

    Plasmid
    #228550
    Purpose
    Template for PCR amplification for gRNA cloning. The PCR product recombines in vivo with a PCRs products from pOliSpec5' and oligo-spacer amplifications, to clone gRNA plasmid.
    Depositor
    Jeferson Gross
    Insert
    Truncated 3' region of aadA
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 2, 2024
    Availability
    Academic Institutions and Nonprofits only
    228550-plasmid-map-sequence-id-454859

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  3. pHR-TRE-miRFP703_PGK-Puro

    Plasmid
    #216667
    Purpose
    Expresses tTa-inducible miRFP703, with puromycin resistance
    Depositor
    Leonardo Morsut
    Inserts
    miRFP703
    PuroR
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Nov. 14, 2024
    Availability
    Academic Institutions and Nonprofits only
    216667-plasmid-map-sequence-id-432197

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  5. pAAV-phSyn-flex-SypHaloTag

    Plasmid
    #172280
    Purpose
    Cre-dependent HaloTag labeling of neuronal presynaptic terminals from the synapsin promoter.
    Depositor
    Danny Winder
    Insert
    synaptophysin-HaloTag
    Use
    AAV and Cre/Lox
    Tags
    HaloTag
    Expression
    Mammalian
    Mutation
    Promoter
    rat synapsin
    Available since
    Sept. 26, 2024
    Availability
    Academic Institutions and Nonprofits only
    172280-plasmid-map-sequence-id-337805

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  6. MDH2_EC

    Plasmid
    #224146
    Purpose
    IPTG induces expression of C. elegans protein MDH-2 with a N-terminal His tag.
    Depositor
    Katherine Walstrom
    Insert
    MDH-2 (mdh-2 Nematode)
    Use
    Tags
    His tag, Xpress tag, enterokinase cleaveage site
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Sept. 17, 2024
    Availability
    Academic Institutions and Nonprofits only
    224146-plasmid-map-sequence-id-444697

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  7. pIND4-GFP-MmXYRS

    Plasmid
    #222745
    Purpose
    Expresses sfGFP and aaRS-tRNA pair for incorporating haloalkane crosslinker
    Depositor
    Steven Boxer
    Inserts
    sfGFP
    MmXYRS
    Mm Pyl tRNA
    Use
    Tags
    6xHis
    Expression
    Mutation
    Contains the following substitutions: V346A, W348…
    Promoter
    rrnB
    Available since
    Sept. 11, 2024
    Availability
    Academic Institutions and Nonprofits only
    222745-plasmid-map-sequence-id-442565

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  8. pGEX-6p1-GST-Ubiquitin

    Plasmid
    #223236
    Purpose
    Bacterial expression construct for GST-Tagged Ubiquitin production and purification
    Depositor
    Weixing Zhao
    Insert
    Ubiquitin
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    tac
    Available since
    Aug. 29, 2024
    Availability
    Academic Institutions and Nonprofits only
    223236-plasmid-map-sequence-id-443802

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  9. H2B-Electra2

    Plasmid
    #218929
    Purpose
    Electra2 fused to human H2B protein for nuclear localization
    Depositor
    Amro Hamdoun
    Insert
    H2B-Electra2 (H2B Human, Entacmaea quadricolor, Synthetic)
    Use
    Synthetic Biology
    Tags
    Electra2 and H2B
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Aug. 6, 2024
    Availability
    Academic Institutions and Nonprofits only
    218929-plasmid-map-sequence-id-433882

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  10. LvPolyUb

    Plasmid
    #218982
    Purpose
    L.variegatus polyubiquitin-C promoter
    Depositor
    Amro Hamdoun
    Insert
    L.variegatus polyubiquitinC promoter
    Use
    Sea urchin
    Tags
    Expression
    Mutation
    Promoter
    L.variegatus polyubiquitinC promoter
    Available since
    Aug. 6, 2024
    Availability
    Academic Institutions and Nonprofits only
    218982-plasmid-map-sequence-id-433881

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  11. pet28a+_Yjjg

    Plasmid
    #220228
    Purpose
    Bacterial expression of Yjjgin E. coli BL21_DE3 (pLys) or similar
    Depositor
    Bernd Nidetzky
    Insert
    Pyrimidine 5'-nucleotidase YjjG (yjjG E.coli)
    Use
    Tags
    Histag
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    July 18, 2024
    Availability
    Academic Institutions and Nonprofits only
    220228-plasmid-map-sequence-id-439539

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  12. MUT_S1-spacer-H1

    Plasmid
    #218399
    Purpose
    3-exon minigene, with SRSF1 motif-rich middle exon, hnRNPA1 motif-rich region in second intron following a neutral spacer, and AG>GG mutation in final 3' splice site
    Depositor
    Oded Regev
    Insert
    Modified CHO DHFR minigene
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    tetracycline-responsive promoter
    Available since
    June 17, 2024
    Availability
    Academic Institutions and Nonprofits only
    218399-plasmid-map-sequence-id-436404

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  13. MUT_S1-H1,NR

    Plasmid
    #218395
    Purpose
    3-exon minigene, with SRSF1 motif-rich middle exon, hnRNPA1 motif-rich region in second intron, both randomly mutated to avoid sequence repeats, and AG>GG mutation in final 3' splice site
    Depositor
    Oded Regev
    Insert
    Modified CHO DHFR minigene
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    tetracycline-responsive promoter
    Available since
    May 17, 2024
    Availability
    Academic Institutions and Nonprofits only
    218395-plasmid-map-sequence-id-433911

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  14. 1167A

    Plasmid
    #218233
    Purpose
    HDR plasmid for inserting Ceratitis capitata transformer female intron into the white pupae gene
    Depositor
    Omar Akbari
    Insert
    putative metabolite transport protein
    Use
    Tags
    Expression
    Insect
    Mutation
    Promoter
    Available since
    May 16, 2024
    Availability
    Academic Institutions and Nonprofits only
    218233-plasmid-map-sequence-id-433264

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  15. pET28-Flag-RBM24

    Plasmid
    #208621
    Purpose
    for E. coli expression of Flag tagged human RBM24 (ENST00000379052)
    Depositor
    Quanfu Ma
    Insert
    human RBM24 (ENST00000379052) coding region (RBM24 Human)
    Use
    Tags
    6xHis and Flag
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    May 15, 2024
    Availability
    Academic Institutions and Nonprofits only
    208621-plasmid-map-sequence-id-433637

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  16. pET-29b(+)_notufA-EmEndoF2-CBD-His

    Plasmid
    #218197
    Purpose
    Bacterial expression of a His-tagged Chitin Binding fusion of Elizabethkingia miricola EndoF2
    Depositor
    Florence Pojer
    Insert
    EndoF2
    Use
    Tags
    CBD-His
    Expression
    Bacterial
    Mutation
    codon optimized Ecoli, residues 45-335
    Promoter
    T7
    Available since
    April 30, 2024
    Availability
    Academic Institutions and Nonprofits only
    218197-plasmid-map-sequence-id-431793

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  17. pAG426-GAL-Prepro-3x-Beta-Amyloid

    Plasmid
    #181730
    Purpose
    Galactose inducible expression of Prepro-3x-Beta-Amyloid
    Depositor
    Alejandro Chavez
    Insert
    Prepro-3x-Beta-Amyloid
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    April 26, 2024
    Availability
    Academic Institutions and Nonprofits only
    181730-plasmid-map-sequence-id-431127

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  18. pAG426-GAL-Kar2-Beta-Amyloid

    Plasmid
    #181707
    Purpose
    Galactose inducible expression of Kar2-Beta-Amyloid
    Depositor
    Alejandro Chavez
    Insert
    Kar2-Beta-Amyloid
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    April 24, 2024
    Availability
    Academic Institutions and Nonprofits only
    181707-plasmid-map-sequence-id-430950

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  19. E3_pENTR_L3-L2_NI_G1333-DddA11-N

    Plasmid
    #191609
    Purpose
    ptpTALECD_v2mod vector assembly, Entry clone 1 with attL2 and L3
    Depositor
    Shin-ichi Arimura
    Insert
    C- and N termini of platinum TALEN, a half of the evolved DddA (DddA11), and a uracil glycosylase inhibitor
    Use
    Other
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Feb. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    191609-plasmid-map-sequence-id-379112

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  20. E3_pENTR_L3-L2_NN_G1333-DddA11-N

    Plasmid
    #191610
    Purpose
    ptpTALECD_v2mod vector assembly, Entry clone 1 with attL2 and L3
    Depositor
    Shin-ichi Arimura
    Insert
    C- and N termini of platinum TALEN, a half of the evolved DddA (DddA11), and a uracil glycosylase inhibitor
    Use
    Other
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Feb. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    191610-plasmid-map-sequence-id-379111

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  21. E1_pENTR_L1-L4_HD_G1333-DddA11-C

    Plasmid
    #191599
    Purpose
    ptpTALECD_v2mod vector assembly, Entry clone 1 with attL1 and L4
    Depositor
    Shin-ichi Arimura
    Insert
    C- and N termini of platinum TALEN, a half of the evolved DddA (DddA11), and a uracil glycosylase inhibitor
    Use
    Other
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Feb. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    191599-plasmid-map-sequence-id-379206

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  22. E1_pENTR_L1-L4_NG_G1333-DddA11-C

    Plasmid
    #191600
    Purpose
    ptpTALECD_v2mod vector assembly, Entry clone 1 with attL1 and L4
    Depositor
    Shin-ichi Arimura
    Insert
    C- and N termini of platinum TALEN, a half of the evolved DddA (DddA11), and a uracil glycosylase inhibitor
    Use
    Other
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Feb. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    191600-plasmid-map-sequence-id-379205

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  23. E1_pENTR_L1-L4_NI_G1333-DddA11-C

    Plasmid
    #191601
    Purpose
    ptpTALECD_v2mod vector assembly, Entry clone 1 with attL1 and L4
    Depositor
    Shin-ichi Arimura
    Insert
    C- and N termini of platinum TALEN, a half of the evolved DddA (DddA11), and a uracil glycosylase inhibitor
    Use
    Other
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Feb. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    191601-plasmid-map-sequence-id-379204

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  24. E1_pENTR_L1-L4_NN_G1333-DddA11-C

    Plasmid
    #191602
    Purpose
    ptpTALECD_v2mod vector assembly, Entry clone 1 with attL1 and L4
    Depositor
    Shin-ichi Arimura
    Insert
    C- and N termini of platinum TALEN, a half of the evolved DddA (DddA11), and a uracil glycosylase inhibitor
    Use
    Other
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Feb. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    191602-plasmid-map-sequence-id-379199

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  25. E3_pENTR_L3-L2_HD_G1333-DddA11-N

    Plasmid
    #191607
    Purpose
    ptpTALECD_v2mod vector assembly, Entry clone 1 with attL2 and L3
    Depositor
    Shin-ichi Arimura
    Insert
    C- and N termini of platinum TALEN, a half of the evolved DddA (DddA11), and a uracil glycosylase inhibitor
    Use
    Other
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Feb. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    191607-plasmid-map-sequence-id-379194

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  26. E3_pENTR_L3-L2_NG_G1333-DddA11-N

    Plasmid
    #191608
    Purpose
    ptpTALECD_v2mod vector assembly, Entry clone 1 with attL2 and L3
    Depositor
    Shin-ichi Arimura
    Insert
    C- and N termini of platinum TALEN, a half of the evolved DddA (DddA11), and a uracil glycosylase inhibitor
    Use
    Other
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Feb. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    191608-plasmid-map-sequence-id-379113

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  27. pT7CFE1_polio_VP12A

    Plasmid
    #212988
    Purpose
    Encodes Poliovirus type I Mahoney VP12A optimized for expression in mammalian transcription/translation extracts
    Depositor
    Karla Kirkegaard
    Insert
    Poliovirus type I Mahoney VP12A
    Use
    Mammalian cell free protein expression
    Tags
    HA tag
    Expression
    Mutation
    Promoter
    T7
    Available since
    Jan. 26, 2024
    Availability
    Academic Institutions and Nonprofits only
    212988-plasmid-map-sequence-id-420361

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  28. pcDNA3-hFCER1G-VenusFlag

    Plasmid
    #211899
    Purpose
    Expressess human FCER1G in mammalian cells
    Depositor
    Haian Fu
    Insert
    FCER1G (FCER1G Human)
    Use
    Tags
    VenusFlag
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Jan. 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    211899-plasmid-map-sequence-id-419135

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  29. pNHA_OROV_NSs

    Plasmid
    #208934
    Purpose
    Expresses N-terminally HA-tagged Oropouche orthobunyavirus NSs protein from CMV promoter in mammalian cells
    Depositor
    Gerald Barry
    Insert
    NSs
    Use
    Tags
    HA
    Expression
    Mammalian
    Mutation
    Deleted 69 bp at 5' end (including ATG) and …
    Promoter
    CMV
    Available since
    Jan. 10, 2024
    Availability
    Academic Institutions and Nonprofits only
    208934-plasmid-map-sequence-id-418959

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  30. pPB-CAG-NPHP1

    Plasmid
    #209769
    Purpose
    Human NPHP1 (Nephrocystin 1) gene is inserted into pPB-PGK-destination (Plasmid #60436).
    Depositor
    Yohei Hayashi
    Insert
    NPHP1 (NPHP1 Human, Synthetic)
    Use
    Piggybac transposon
    Tags
    HA tag
    Expression
    Mammalian
    Mutation
    Promoter
    CAG
    Available since
    Jan. 2, 2024
    Availability
    Academic Institutions and Nonprofits only
    209769-plasmid-map-sequence-id-417213

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  31. pCR8GW-HA-Wnk

    Plasmid
    #203967
    Purpose
    HA-Wnk basic construct
    Depositor
    Andreas Jenny
    Insert
    Wnk (most similar to RO variant) (Wnk Fly)
    Use
    Unspecified
    Tags
    HA
    Expression
    Mutation
    Promoter
    Available since
    Oct. 4, 2023
    Availability
    Academic Institutions and Nonprofits only
    203967-plasmid-map-sequence-id-403297

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  32. pENTR3cDual-Fray

    Plasmid
    #203977
    Purpose
    Enrty clone for Gateway cloning
    Depositor
    Andreas Jenny
    Insert
    Frayed (fray Fly)
    Use
    Unspecified
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Oct. 4, 2023
    Availability
    Academic Institutions and Nonprofits only
    203977-plasmid-map-sequence-id-403301

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  34. pAG416GAL10-POWV_NS2B-GS-NS3

    Plasmid
    #203471
    Purpose
    Expresses POWV NS2B-NS3 protease (with GS linker) from a GAL10 promoter with a URA3 marker
    Depositor
    Alejandro Chavez
    Insert
    POWV NS2B-GS-NS3 (POLY )
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    GAL10
    Available since
    Aug. 9, 2023
    Availability
    Academic Institutions and Nonprofits only
    203471-plasmid-map-sequence-id-403573

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  35. pAG416GAL10-LGTV_NS2B-GS-NS3

    Plasmid
    #203468
    Purpose
    Expresses LGTV NS2B-NS3 protease (with GS linker) from a GAL10 promoter with a URA3 marker
    Depositor
    Alejandro Chavez
    Insert
    LGTV NS2B-GS-NS3 (POLY )
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    GAL10
    Available since
    Aug. 9, 2023
    Availability
    Academic Institutions and Nonprofits only
    203468-plasmid-map-sequence-id-403572

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  36. pAG416GAL10-YES1-6stop

    Plasmid
    #203476
    Purpose
    Expresses human YES1 kinase from a GAL10 promoter with a URA3 marker
    Depositor
    Alejandro Chavez
    Insert
    YES1 (YES1 Human)
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    GAL10
    Available since
    July 31, 2023
    Availability
    Academic Institutions and Nonprofits only
    203476-plasmid-map-sequence-id-402869

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  37. pAG416GAL-SARS-CoV_PLP

    Plasmid
    #201942
    Purpose
    Expresses SARS-CoV papain-like protease from a GAL promoter with a URA3 marker
    Depositor
    Alejandro Chavez
    Insert
    SARS-CoV PLP (ORF1ab )
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    GAL1
    Available since
    July 31, 2023
    Availability
    Academic Institutions and Nonprofits only
    201942-plasmid-map-sequence-id-402854

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  38. pAG426GAL-MERS-CoV_PLP

    Plasmid
    #203508
    Purpose
    Expresses MERS-CoV papain-like protease from a GAL promoter with a URA3 marker
    Depositor
    Alejandro Chavez
    Insert
    MERS-CoV PLP (orf1ab )
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    GAL1
    Available since
    July 31, 2023
    Availability
    Academic Institutions and Nonprofits only
    203508-plasmid-map-sequence-id-402806

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  39. pAG416GAL10-CCHFV_OTU

    Plasmid
    #201945
    Purpose
    Expresses CCHFV OTU domain of L protein from a GAL10 promoter with a URA3 marker
    Depositor
    Alejandro Chavez
    Insert
    CCHFV OTU domain of L protein (RdRp )
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    GAL10
    Available since
    July 31, 2023
    Availability
    Academic Institutions and Nonprofits only
    201945-plasmid-map-sequence-id-402790

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  40. pAG416GALL-SARS-CoV_3CL

    Plasmid
    #203495
    Purpose
    Expresses SARS-CoV 3CL protease from a GALL promoter with a URA3 marker
    Depositor
    Alejandro Chavez
    Insert
    SARS-CoV 3CL protease (ORF1ab )
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    GALL
    Available since
    July 31, 2023
    Availability
    Academic Institutions and Nonprofits only
    203495-plasmid-map-sequence-id-402789

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Showing: 3961 - 4000 of 8321 results