We narrowed to 54,354 results for: plasmid

1. pDK714

   Plasmid

   #134230

   Purpose

   Mammalian expresion of tdTomato SKAP

   Depositor

   Iain Cheeseman

   Insert

   DK365 tdTomato (short SKAP) (KNSTRN Human)
   

   Tags

   tdTomato

   Expression

   Mammalian

   Available Since

   Feb. 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. cTRE-LSL-GFP-IRES-Cas9-sgCR8

   Plasmid

   #135668

   Purpose

   Introduce Dox/Cre-inducible (TRE promoter followed by lox-stop-lox) Cas9 cDNA plus control-targeting sgRNA into (ES) cells by recombination-mediated cassette exchange

   Depositor

   Florian Karreth

   Insert

   sgCR8
   

   Use

   Mouse Targeting

   Available Since

   Jan. 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. Z-MATR3-4

   Plasmid

   #166558

   Purpose

   scFv of a human scaffold targeting Matrin-3. Antigen coverage aa 390-578 of 847

   Depositor

   Susanne Gräslund

   Insert

   single chain-Fv, scFv
   

   Use

   Recombinant antibody expression

   Tags

   3xFLAG, His6 and OmpA leader sequence

   Expression

   Bacterial

   Promoter

   LacZ

   Available Since

   April 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLV CDHblast OST-Myorg-V5

   Plasmid

   #175171

   Purpose

   Lentiviral expression of mouse Myorg-V5

   Depositor

   Larry Gerace

   Insert

   Myorg (Myorg Mouse)
   

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mammalian

   Available Since

   Jan. 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLV CDHblast OST-Myorg(D462A)-V5

   Plasmid

   #175172

   Purpose

   Lentiviral expression of mouse Myorg-V5

   Depositor

   Larry Gerace

   Insert

   Myorg (Myorg Mouse)
   

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mammalian

   Mutation

   D462A

   Available Since

   Jan. 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pSNAI2-donor

   Plasmid

   #112378

   Purpose

   CRISPR donor plasmid to tag human transcription factor SNAI2 with GFP

   Depositor

   Kevin White

   Insert

   SNAI2 homology arms flanking EGFP-IRES-Neo cassette (SNAI2 Human)
   

   Use

   CRISPR

   Available Since

   Dec. 3, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pGEM-T ZfHoxd12a

   Plasmid

   #27545

   Depositor

   Victoria Prince

   Insert

   Hoxd12a (hoxd12a Zebrafish)
   

   Expression

   Bacterial

   Available Since

   Feb. 16, 2011

   Availability

   Academic Institutions and Nonprofits only

8. pZBTB9-donor

   Plasmid

   #112373

   Purpose

   CRISPR donor plasmid to tag human transcription factor ZBTB9 with GFP

   Depositor

   Kevin White

   Insert

   ZBTB9 homology arms flanking EGFP-IRES-Neo cassette (ZBTB9 Human)
   

   Use

   CRISPR

   Mutation

   Unknown

   Available Since

   Dec. 3, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pZNF436-donor

   Plasmid

   #113800

   Purpose

   CRISPR donor plasmid to tag human transcription factor ZNF436 with GFP

   Depositor

   Kevin White

   Insert

   ZNF436 homology arms flanking EGFP-IRES-Neo cassette (ZNF436 Human)
   

   Use

   CRISPR

   Mutation

   rs2849028, rs646193

   Available Since

   Dec. 4, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. SLC2A3

    Plasmid

    #74649

    Purpose

    Baculovirus expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    SLC2A3 (SLC2A3 Human)
    

    Use

    Baculovirus

    Tags

    TEV-His10-Flag

    Promoter

    PH

    Available Since

    April 21, 2016

    Availability

    Academic Institutions and Nonprofits only

11. A-WDR5-1

    Plasmid

    #166515

    Purpose

    scFv of a human scaffold targeting WD repeat domain 5. Antigen coverage aa 1-334 of 334.

    Depositor

    Susanne Gräslund

    Insert

    single chain-Fv, scFv
    

    Use

    Recombinant antibody expression

    Tags

    3xFLAG, His6 and OmpA leader sequence

    Expression

    Bacterial

    Promoter

    LacZ

    Available Since

    April 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pJW1328

    Plasmid

    #69488

    Purpose

    sgRNA(F+E) targeting pha-1 (no Cas9); for co-conversion in C. elegans

    Depositor

    Jordan Ward

    Insert

    sgRNA(F+E) targeting pha-1
    

    Expression

    Worm

    Promoter

    R07E5.16 U6 promoter

    Available Since

    Sept. 30, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. pBac[3xP3-gTc'v; Tc'aTub1P-loxP(mCherry)Sv40_Tc'H2Av_EGFP]

    Plasmid

    #86456

    Purpose

    Lox responder construct with floxed (same orientation; 2xloxP, 2xlox2272) mCherry (cy) body marker and H2Av::EGFP (nu) for Cre dependent cell clone marking; (Transformation plasmid; Black eye marker)

    Depositor

    Gregor Bucher

    Insert

    3xP3-gTc'v; Tc'aTub1P-loxP(mCherry)Sv40_Tc'H2Av_EGFP
    

    Expression

    Insect

    Available Since

    March 22, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. p-dCas9-SET7-H297G-Hygro

    Plasmid

    #104415

    Purpose

    transient expression of dCas9-dSET7 fusion protein

    Depositor

    Stephan Beck

    Insert

    dSET7
    

    Use

    CRISPR

    Expression

    Mammalian

    Mutation

    H297G

    Promoter

    CMV promoter

    Available Since

    April 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCB99

    Plasmid

    #69745

    Purpose

    Retroviral expression of Centrin 2

    Depositor

    Iain Cheeseman

    Insert

    Centrin 2 (CETN2 Human)
    

    Use

    Retroviral

    Tags

    eGFP-TEV-S

    Expression

    Mammalian

    Available Since

    Nov. 4, 2015

    Availability

    Academic Institutions and Nonprofits only

16. PHIPA

    Plasmid

    #74665

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    PHIPA (PHIP Human)
    

    Tags

    His-6-TEV

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    April 21, 2016

    Availability

    Academic Institutions and Nonprofits only

17. pDK713

    Plasmid

    #134229

    Purpose

    Mammalian expresion of tdTomato SKAP

    Depositor

    Iain Cheeseman

    Insert

    DK360 tdTomato (long SKAP) (KNSTRN Human)
    

    Tags

    tdTomato

    Expression

    Mammalian

    Available Since

    Feb. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pSPDEF-donor

    Plasmid

    #113828

    Purpose

    CRISPR donor plasmid to tag human transcription factor SPDEF with GFP

    Depositor

    Kevin White

    Insert

    SPDEF homology arms flanking EGFP-IRES-Neo cassette (SPDEF Human)
    

    Use

    CRISPR

    Available Since

    Dec. 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCB98

    Plasmid

    #69744

    Purpose

    Retroviral expression of Centrin 1

    Depositor

    Iain Cheeseman

    Insert

    Centrin 1 (CETN1 Human)
    

    Use

    Retroviral

    Tags

    eGFP-TEV-S

    Expression

    Mammalian

    Available Since

    Nov. 4, 2015

    Availability

    Academic Institutions and Nonprofits only

20. pTFCP2-donor

    Plasmid

    #112308

    Purpose

    CRISPR donor plasmid to tag human transcription factor TFCP2 with GFP

    Depositor

    Kevin White

    Insert

    TFCP2 homology arms flanking EGFP-IRES-Neo cassette (TFCP2 Human)
    

    Use

    CRISPR

    Available Since

    Dec. 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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