We narrowed to 7,241 results for: Cit

1. pX330-Flag-deSpCas9

   Plasmid

   #92114

   Purpose

   Expression plasmid for human codon-optimized dead/inactive increased fidelity eSpCas9 (1.1) (without U6-sgRNA coding sequence)

   Depositor

   Ervin Welker

   Article

   Kulcsar et al Genome Biol. 2017 Oct 6;18(1):190. doi:

   Insert

   dead/inactive eSpCas9 with FLAG tag
   

   Use

   CRISPR

   Tags

   3xFLAG and NLS

   Expression

   Mammalian

   Mutation

   D10A, H840A, K848A, K1003A, R1060A

   Promoter

   Cbh

   Available since

   Oct. 31, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAV-CaMKIIa-DIO ChRmine-oScarlet-KV 2.1-WPRE

   Plasmid

   #183536

   Purpose

   Optogenetics

   Depositor

   Karl Deisseroth

   Article

   Fan et al Cell. 2023 Feb 2;186(3):543-559.e19. doi

   Insert

   ChRmine-oScarlet-Kv2.1
   

   Use

   AAV

   Tags

   Expression

   Mutation

   None

   Promoter

   CaMKIIa

   Available since

   Feb. 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pHH0103_HCLS1-1/1

   Plasmid

   #91437

   Purpose

   Protein expression and purification of human SH3 domain construct HCLS1-1/1

   Depositor

   Sachdev Sidhu

   Article

   Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

   Insert

   HCLS1-1/1 (HCLS1 Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 19, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLV-CMV-hXRCC1-mCherry-Hygro

   Plasmid

   #176532

   Purpose

   mCherry fused to the C-terminus of XRCC1 & a hygromycin resistance cassette

   Depositor

   Robert Sobol

   Article

   Wilk et al Sci Rep. 2020 Jan 20;10(1):651. doi: 10.

   Insert

   X-Ray Repair Cross Complementing 1 (XRCC1 Human)
   

   Use

   Lentiviral

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pKD279.8

   Plasmid

   #173171

   Purpose

   Encodes expression of SO_4388(REC)-PsdR(DBD) 137 and an sfGFP reporter

   Depositor

   Jeffrey Tabor

   Article

   Cartwright et al Proc Natl Acad Sci U S A. 2021 May 18;11

   Inserts

   SO_4388(REC)-PsdR(DBD) 137
   sfGFP
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   J23108 and PpsdA110

   Available since

   Sept. 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. ELF3_pet28a

   Plasmid

   #131658

   Purpose

   Bacterial expression of ETS gene ELF3

   Depositor

   Peter Hollenhorst

   Article

   Selvaraj et al Cell Commun Signal. 2015 Feb 19;13:12. d

   Insert

   ELF3 (ELF3 Human)
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET28a-SENP6 (catalytic domain)

   Plasmid

   #16359

   Depositor

   Guy Salvesen

   Article

   Mikolajczyk et al J Biol Chem. 2007 Sep 7. 282(36):26217-2

   Insert

   human SENP6 catalytic domain (SENP6 Human)
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Amino Acid 628 to 1112 catalytic domain of human …

   Promoter

   Available since

   Dec. 19, 2007

   Availability

   Academic Institutions and Nonprofits only

8. CMV-ShadowY-CBD-P2A-Clover(T153M/F223R)-Cdc42

   Plasmid

   #165434

   Purpose

   Expresses ShadowY-CBD and Clover(T153M/F223R)-Cdc42 in mammalian cells to monitor Cdc42 activity with FLIM imaging.

   Depositor

   Hideji Murakoshi

   Article

   Shibata et al Nat Commun. 2021 Feb 2;12(1):751. doi: 1

   Insert

   ShadowY-CBD-P2A-Clover(T153M/F223R)-Cdc42
   

   Use

   Tags

   ShadowY, Clover(T153M/F223R)

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLC-HA active Nedd8-P2A-Hygro

   Plasmid

   #124307

   Purpose

   Lentiviral vector for expression of HA tagged active or C-terminal cleaved Nedd8-P2A-Hygro casette from a CMV promoter

   Depositor

   Eva Gottwein

   Article

   Patil et al Blood Adv. 2019 Jul 23;3(14):2105-2117.

   Insert

   Nedd8 (NEDD8 Human)
   

   Use

   Lentiviral

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Five C-terminal amino acids missing compared to w…

   Promoter

   CMV

   Available since

   July 22, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAAV-4mtD3cpv

    Plasmid

    #181874

    Purpose

    Expresses the FRET-based mitochondrial calcium indicator 4mtD3cpv under control of the CaMKIIa promoter

    Depositor

    Hilmar Bading

    Article

    Hagenston et al J Biol Chem. 2021 Dec 20:101508. doi: 10

    Insert

    4mtD3cpv
    

    Use

    AAV and Adenoviral

    Tags

    Expression

    Mammalian

    Mutation

    the 4mtD3cpv CDS differs from the recorded GeneBa…

    Promoter

    CaMKIIa

    Available since

    March 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. TRAV8-1

    Plasmid

    #123375

    Purpose

    construction of TCR

    Depositor

    Catherine Wu

    Article

    Hu et al Blood. 2018 Nov 1;132(18):1911-1921. doi

    Insert

    TRAV8-1 (TRAV8-1 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    WT

    Promoter

    Available since

    May 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLC-CUL4A WT-P2A-Puro

    Plasmid

    #124304

    Purpose

    Lentiviral vector for expression of wild-type CUL4A-P2A-Puro casette from a CMV promoter

    Depositor

    Eva Gottwein

    Article

    Patil et al Blood Adv. 2019 Jul 23;3(14):2105-2117.

    Insert

    CUL4A (CUL4A Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pYPQ265F2

    Plasmid

    #164722

    Purpose

    Gateway entry clone (attL1 & attR5) A3A/Y130F-nCas9-NG-CBE_V01 for C-T base editing

    Depositor

    Yiping Qi

    Article

    Ren et al Plant Biotechnol J. 2021 May 27. doi: 10

    Insert

    A3A(Y130F)-zCas9(D10A)NG-UGI
    

    Use

    CRISPR; Gateway compatible a3a/y130f-ncas9-ng-cbe…

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    May 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pTE2690

    Plasmid

    #137717

    Purpose

    Expresses dimpLbCas12a-SunTag in mammalian cells.

    Depositor

    Ervin Welker

    Article

    Toth et al Nucleic Acids Res. 2020 Feb 28. pii: 576

    Insert

    dimpLbCas12a
    

    Use

    CRISPR

    Tags

    NLSx3 SV40 and SunTagx10

    Expression

    Mammalian

    Mutation

    D156R, G532R, K538V, Y542R, K595R, D832A,

    Promoter

    CMV

    Available since

    March 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. LV_EF1a_CDK4-P2A-Hygro_Barcode

    Plasmid

    #170223

    Purpose

    Barcoded lentiviral vector to express CDK4 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq

    Depositor

    Prashant Mali

    Article

    Parekh et al iScience. 2021 Sep 20;24(10):103149. doi

    Insert

    CDK4 (CDK4 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    EF1a

    Available since

    Sept. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. EHF_pet28a

    Plasmid

    #131660

    Purpose

    Bacterial expression of ETS gene EHF

    Depositor

    Peter Hollenhorst

    Article

    Selvaraj et al Cell Commun Signal. 2015 Feb 19;13:12. d

    Insert

    EHF (EHF Human)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. HTRA1_PDZ_1

    Plasmid

    #103912

    Purpose

    Protein expression and purification of HTRA1 PDZ domain

    Depositor

    Sachdev Sidhu

    Article

    Tonikian et al PLoS Biol. 2008 Sep 30;6(9):e239. doi: 1

    Insert

    HTRA1 (HTRA1 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    tac

    Available since

    Aug. 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pGW011b Dual AAV vector pAAV-EFS-dCAS9-spA

    Plasmid

    #192160

    Purpose

    Dual AAV vector AAV-dCas9

    Depositor

    Sidi Chen

    Article

    Wang et al Nat Immunol. 2019 Nov;20(11):1494-1505.

    Insert

    Dead Cas9
    

    Use

    AAV

    Tags

    Expression

    Mutation

    NA

    Promoter

    Available since

    Oct. 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pDEST-Tol2(Chien)-Krt4:Gal4VP16:tdTomato:5xUAS:HyPer:pA

    Plasmid

    #105643

    Purpose

    Co-expression of tdTomato and HyPer in differentiated keratinocytes of the periderm layer and ealy on also in the basal layer of the epidermis

    Depositor

    Sandra Rieger

    Article

    Lisse et al Proc Natl Acad Sci U S A. 2016 Apr 12;11

    Insert

    Krt4:Gal4VP16:tdTomato:5xUAS:HyPer:pA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 2, 2018

    Availability

    Academic Institutions and Nonprofits only

20. pHH0103_CSK-1/1

    Plasmid

    #91542

    Purpose

    Protein expression and purification of human SH3 domain construct CSK-1/1

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

    Insert

    CSK-1/1 (CSK Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. GABPA_pet28a

    Plasmid

    #131640

    Purpose

    Bacterial expression of ETS gene GABPA

    Depositor

    Peter Hollenhorst

    Article

    Selvaraj et al Cell Commun Signal. 2015 Feb 19;13:12. d

    Insert

    GABPA (GABPA Human)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. DLG4_PDZ_3

    Plasmid

    #103953

    Purpose

    Protein expression and purification of PSD95 PDZ3 domain

    Depositor

    Sachdev Sidhu

    Article

    Tonikian et al PLoS Biol. 2008 Sep 30;6(9):e239. doi: 1

    Insert

    DLG4 (DLG4 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    tac

    Available since

    Aug. 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pET28b-BE3∆UGI

    Plasmid

    #96943

    Purpose

    The plasmid encoding the His6-rAPOBEC1-XTEN-nCas9 protein (BE3∆UGI) was generated by site-directed mutagenesis using pET28b-BE1 (Addgene plasmid #73018).

    Depositor

    Daesik Kim

    Article

    Kim et al Nat Biotechnol. 2017 May;35(5):475-480.

    Insert

    BE3∆UGI
    

    Use

    Tags

    His6 tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Sept. 20, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pOPINK-OTUD4 (OTU, aa 22-196)

    Plasmid

    #61413

    Purpose

    Expresses human OTUD4 (OTU domain) in E. coli.

    Depositor

    David Komander

    Article

    Mevissen et al Cell. 2013 Jul 3;154(1):169-84. doi: 10.

    Insert

    OTUD4 (OTU domain-containing protein 4) (OTUD4 Human)
    

    Use

    Tags

    His6-GST-3C

    Expression

    Bacterial

    Mutation

    Deleted aa 1-21 and aa 197-1114.

    Promoter

    T7

    Available since

    March 12, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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25. pcDNA3.1 3xFLAG TPP1 88-544 delta166-172

    Plasmid

    #53551

    Purpose

    mammalian expression of TPP1 full length with 166-172 replaced with "GSSG"

    Depositor

    Kathleen Collins

    Article

    Sexton et al J Biol Chem. 2012 Oct 5;287(41):34455-64

    Insert

    TPP1 (ACD Human)
    

    Use

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    contains aa 88-544, with aa 166-172 replaced wit…

    Promoter

    CMV

    Available since

    June 3, 2014

    Availability

    Academic Institutions and Nonprofits only

26. pUAST cyto Grx1-roGFP2

    Plasmid

    #64994

    Purpose

    for expression of cytosolic Grx1-roGFP2 with the Gal4 expression system in drosophila

    Depositor

    Tobias Dick

    Article

    Albrecht et al Cell Metab. 2011 Dec 7;14(6):819-29. doi

    Insert

    Glutaredoxin-1 (GLRX Human)
    

    Use

    Tags

    roGFP2

    Expression

    Insect

    Mutation

    Promoter

    Available since

    July 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pT5T_hp53_ST

    Plasmid

    #34922

    Depositor

    Thanos Halazonetis

    Article

    Petty et al EMBO J. 2011 Jun 1;30(11):2167-76. Epub

    Insert

    p53 DNA-binding (Res 1-393) (TP53 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    C135V, C141V, W146Y, C182S, V203A, R209P, C229Y, …

    Promoter

    T7

    Available since

    Feb. 22, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. SNX25-PX (506-628)

    Plasmid

    #119104

    Purpose

    Bacterial expression of human phox homology (PX) domain, SNX25-PX (506-628)

    Depositor

    Brett Collins

    Article

    Chandra et al Nat Commun. 2019 Apr 4;10(1):1528. doi:

    Insert

    SNX25-PX (506-628) (SNX25 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    tac

    Available since

    April 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pOPINK-Cezanne2 (OTU+UBA, aa 1-462)

    Plasmid

    #61582

    Purpose

    Expresses human Cezanne2 (OTU+UBA domains) in E. coli.

    Depositor

    David Komander

    Article

    Mevissen et al Cell. 2013 Jul 3;154(1):169-84. doi: 10.

    Insert

    Cezanne2 (OTUD7A Human)
    

    Use

    Tags

    His6-GST-3C

    Expression

    Bacterial

    Mutation

    Isoform 2. Deleted aa 463-933.

    Promoter

    T7

    Available since

    March 12, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pX330-Flag-dSpCas9-HF1

    Plasmid

    #92115

    Purpose

    Expression plasmid for human codon-optimized dead/inactive high-fidelity SpCas9-HF1 (without U6-sgRNA coding sequence)

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Genome Biol. 2017 Oct 6;18(1):190. doi:

    Insert

    dead/inactive SpCas9-HF1 with FLAG tag
    

    Use

    CRISPR

    Tags

    3xFLAG and NLS

    Expression

    Mammalian

    Mutation

    D10A, N497A, R661A, Q695A, H840A, Q926A

    Promoter

    Cbh

    Available since

    Oct. 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge