We narrowed to 159,093 results for: Des

Showing: 4001 - 4040 of 159093 results

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GFP-FAK W266A

Plasmid

#186149

Purpose

For expressing FAK in mammalian cells

Depositor

Michael Rosen

Article

Case et al Elife. 2022 Jan 20;11. pii: 72588. doi:

Insert

PTK2 (PTK2 Mouse)

Tags

mEGFP

Expression

Mammalian

Promoter

cmv

Available Since

July 1, 2022

Availability

Academic Institutions and Nonprofits only

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pFLAG-HHARI

Plasmid

#17450

Depositor

Dong-Er Zhang

Article

Okumura et al Genes Dev. 2007 Feb 1. 21(3):255-60.

Insert

HHARI (ARIH1 Human)

Tags

Flag

Expression

Mammalian

Available Since

March 5, 2008

Availability

Academic Institutions and Nonprofits only

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CMV-OMM-GCaMP6f

Plasmid

#127874

Purpose

Fluorescent reporter for mitochondrial outer membrane Ca2+

Depositor

Timothy Ryan

Article

Ashrafi et al Neuron. 2019 Dec 10. pii: S0896-6273(19)

Insert

Outer membrane localized GCaMP6f

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pIVEX-SNAP-GFP

Plasmid

#46846

Purpose

SNAP-GFP fusion

Depositor

Florian Hollfelder

Article

Diamante et al Protein Eng Des Sel. 2013 Oct;26(10):713

Insert

SNAP-GFP fusion

Use

In vitro expression

Available Since

Aug. 12, 2013

Availability

Academic Institutions and Nonprofits only

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pDRF1-GW ymNeongreen-T2A-mCherry

Plasmid

#125700

Purpose

Produces equimolar expression of yeast codon optimized mNeongreen and mCherry

Depositor

Bas Teusink

Article

Botman et al Sci Rep. 2019 Feb 19;9(1):2234. doi: 10.

Insert

ymNeongreen-T2A-mCherry

Expression

Yeast

Available Since

June 4, 2019

Availability

Academic Institutions and Nonprofits only

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pCAG ERT2-Cre-ERT2 lox

Plasmid

#183447

Purpose

CreOFF tamoxifen-inducible Cre

Depositor

Harold MacGillavry

Article

Droogers et al eNeuro. 2022 Jul 18;9(4):ENEURO.0056-22.

Insert

ERT2-Cre-ERT2

Expression

Mammalian

Available Since

April 25, 2022

Availability

Academic Institutions and Nonprofits only

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pCTCON2-GFP

Plasmid

#158130

Purpose

Single fluorescent protein reporter (GFP) for ncAA incorporation (wild-type construct)

Depositor

James Van Deventer

Article

Potts et al Mol Syst Des Eng. 2020 Feb 1;5(2):573-58

Insert

sfGFP

Expression

Yeast

Promoter

GAL1-10

Available Since

Jan. 12, 2021

Availability

Academic Institutions and Nonprofits only

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pRN114

Plasmid

#127221

Purpose

BeYDV replicon with constitutive Luc expression, WUS2 and AtSTM

Depositor

Daniel Voytas

Article

Maher et al Nat Biotechnol. 2019 Dec 16. pii: 10.103

Insert

Luc, Wus, AtSTM

Expression

Plant

Mutation

codon optimized CDSs

Available Since

Dec. 17, 2019

Availability

Academic Institutions and Nonprofits only

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pUF-Cas9-pre-sgRNA

Plasmid

#137845

Purpose

Cas9 and gRNA expression plasmid for P. falciparum with yDHODH selectable marker.

Depositor

Josh Beck

Article

Nessel et al Cell Microbiol. 2020 Jan 28:e13168. doi:

Type

Empty backbone

Use

CRISPR

Available Since

May 18, 2020

Availability

Academic Institutions and Nonprofits only

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pIA1414

Plasmid

#172402

Purpose

Expression of SARS-COV-2 Nsp9 in E. coli

Depositor

Irina Artsimovitch

Article

Wang et al Nucleic Acids Res. 2021 Aug 5. pii: 6342

Insert

SARS-COV-2 Nsp9 (ORF1ab Synthetic)

Tags

His-SUMO

Expression

Bacterial

Promoter

T7

Available Since

Aug. 6, 2021

Availability

Academic Institutions and Nonprofits only

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px459V2.0_Conc2

Plasmid

#134451

Purpose

Empty concatemer vector in which 2 sgRNAs can be inserted; with Cas9 + Puro

Depositor

Daniel Stange

Article

Gaebler et al J Vis Exp. 2020 Feb 18;(156). doi: 10.37

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Jan. 21, 2020

Availability

Academic Institutions and Nonprofits only

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pDRF1 ymScarletI3_T2A_mTurquoise2

Plasmid

#219829

Purpose

equimolar expression of ymScarletI3 and mTurquoise2 in budding yeast

Depositor

Bas Teusink

Article

Botman et al bioRxiv 2025.06.27.661975; doi: https://

Insert

ymScarletI3_T2A_mTurquoise2

Expression

Yeast

Available Since

Aug. 27, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pPPAT-EGFP

Plasmid

#99105

Purpose

Expresses human PPAT-EGFP fusion in mammalian cells

Depositor

Stephen Benkovic

Article

An et al Science. 2008 Apr 4;320(5872):103-6. doi

Insert

PPAT (PPAT Human)

Tags

EGFP (enhanced green fluorescent protein)

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 3, 2017

Availability

Academic Institutions and Nonprofits only

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pMKV059

Plasmid

#133314

Purpose

BeYDV viral replicon on T-DNA backbone expressing Firefly Luc+ and IPT

Depositor

Daniel Voytas

Article

Maher et al Nat Biotechnol. 2019 Dec 16. pii: 10.103

Insert

Luc+, IPT

Expression

Plant

Promoter

AtUbi10, 35S

Available Since

Jan. 29, 2020

Availability

Academic Institutions and Nonprofits only

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PBAD-his6-prkA-pACYC184

Plasmid

#41041

Depositor

Ichiro Matsumura

Article

Parikh et al Protein Eng Des Sel. 2006 Mar;19(3):113-

Insert

prkA

Tags

6xHis and T7 epitope tag

Expression

Bacterial

Promoter

PBAD

Available Since

Nov. 6, 2012

Availability

Academic Institutions and Nonprofits only

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pMSCV-loxp-dsRed-loxp-3xHA-Puro-WPRE

Plasmid

#32703

Depositor

Hans Clevers

Article

Koo et al Nat Methods. 2011 Dec 4. doi: 10.1038/nm

Type

Empty backbone

Use

Cre/Lox and Retroviral

Tags

3x HA tags

Expression

Mammalian

Available Since

Jan. 6, 2012

Availability

Academic Institutions and Nonprofits only

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Ube2v2

Plasmid

#31430

Depositor

Rachel Klevit

Article

Christensen et al Nat Struct Mol Biol. 2007 Oct . 14(10):9

Insert

Ubiquitin Conjugating Enzyme (UBE2V2 Human)

Tags

HA

Expression

Yeast

Available Since

Oct. 27, 2011

Availability

Academic Institutions and Nonprofits only
pDRF1-GW yoeGFP-T2A-mCherry

Plasmid

#118446

Purpose

Produces equimolar expression of yeast codon-optimized eGFP and mCherry

Depositor

Bas Teusink

Article

Botman et al Sci Rep. 2019 Feb 19;9(1):2234. doi: 10.

Insert

yoeGFP-T2A-mCherry

Expression

Yeast

Available Since

June 3, 2019

Availability

Academic Institutions and Nonprofits only

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MCP-EGFP-PiggyBac

Plasmid

#190003

Purpose

Expression of MCP-EGFP in mammalian cells. To clone this plasmid, Addgene plasmid #119908 was used. We replaced the tandem coat proteins and double tagRFP with a single coat protein fused to EGFP.

Depositor

Tineke Lenstra

Article

Brouwer et al Methods Mol Biol. 2024;2694:235-265. doi

Insert

MCP-EGFP

Available Since

Nov. 7, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
CH-GECO2.1

Plasmid

#52099

Purpose

mCherry-based Calcium indicators

Depositor

Robert Campbell

Article

Carlson et al Protein Eng Des Sel. 2013 Dec;26(12):763

Insert

CH-GECO2.1

Tags

6 His tag

Expression

Bacterial

Promoter

pBAD promotor

Available Since

April 14, 2014

Availability

Academic Institutions and Nonprofits only
pLVX-Cry2olig

Plasmid

#208780

Purpose

Inducibly expresses light activatable Cry2 domain

Depositor

Petr Broz

Article

Shkarina et al J Cell Biol. 2022 Jun 6;221(6). pii: 213

Insert

Cry2 mCherry

Use

Lentiviral

Expression

Mammalian

Available Since

Nov. 2, 2023

Availability

Academic Institutions and Nonprofits only

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pLV_Stargazin-mTurquoise2-iLID

Plasmid

#161001

Purpose

Mammalian expression of mTurquoise2-tagged iLID anchored to the plasma membrane via N-terminal fusion to Stargazin for slow diffusion

Depositor

Sean R Collins

Article

Natwick et al ACS Synth Biol. 2021 May 21;10(5):1009-1

Insert

Stargazin-mTurquoise2-iLID

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

Nov. 16, 2020

Availability

Academic Institutions and Nonprofits only

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pMKV058

Plasmid

#133313

Purpose

BeYDV viral replicon on T-DNA backbone expressing Firefly Luc+ and WUS2

Depositor

Daniel Voytas

Article

Maher et al Nat Biotechnol. 2019 Dec 16. pii: 10.103

Insert

Luc+. WUS2

Expression

Plant

Promoter

AtUbi10, Nos

Available Since

Dec. 18, 2019

Availability

Academic Institutions and Nonprofits only

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TRF2-IRESS-eGFP

Plasmid

#19798

Depositor

Mark Mattson

Article

van Steensel et al Cell. 1998 Feb 6. 92(3):401-13.

Insert

TRF2 (TERF2 Human)

Expression

Mammalian

Mutation

When compared to GenBank reference sequence NP_00…

Promoter

CMV-F

Available Since

Nov. 21, 2008

Availability

Academic Institutions and Nonprofits only
pREDusk-AmpR-MCS

Plasmid

#188974

Purpose

For red light-repressed bacterial expression of gene of interest (AmpR)

Depositor

Heikki Takala

Article

Multamaki et al ACS Synth Biol. 2022 Aug 23. doi: 10.102

Type

Empty backbone

Expression

Bacterial

Available Since

Sept. 26, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pREDawn-StrR-MCS

Plasmid

#188979

Purpose

For red light-induced bacterial expression of gene of interest (StrR)

Depositor

Heikki Takala

Article

Multamaki et al ACS Synth Biol. 2022 Aug 23. doi: 10.102

Type

Empty backbone

Expression

Bacterial

Available Since

Sept. 26, 2022

Availability

Academic Institutions and Nonprofits only

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MSGV Hu Acceptor

Plasmid

#64269

Purpose

Retroviral golden gate cloning vector for expression of functional Human T-Cell Receptor genes

Depositor

David Ott

Article

Coren et al Biotechniques. 2015 Mar 1;58(3):135-9. d

Type

Empty backbone

Use

Retroviral

Tags

none

Available Since

April 29, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pPROK AdoHcyase

Plasmid

#14683

Depositor

Michael Hershfield

Article

Coulter-Karis et al Ann Hum Genet. 1989 May . 53(Pt 2):169-7

Insert

adenosyl-homocysteine hydrolase (AHCY Human)

Expression

Bacterial

Available Since

May 1, 2007

Availability

Academic Institutions and Nonprofits only
pFA6a-ymScarletI-CaURA3

Plasmid

#118457

Purpose

Yeast tagging vector to create a gene fusion with yeast optimized mScarletI with CaUra3 selection. *An updated version of this plasmid is available: #168055 pFA6a-link-ymScarletI-URA3.

Depositor

Bas Teusink

Article

Botman et al Sci Rep. 2019 Feb 19;9(1):2234. doi: 10.

Insert

ymScarletI

Expression

Yeast

Available Since

Feb. 26, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pFA6a-ymNeongreen-CaURA3

Plasmid

#125703

Purpose

Yeast tagging vector to create a gene fusion with yeast optimized mNeongreen with CaUra3 selection. *An updated version of this plasmid is available: #168059 pFA6a-link-ymNeongreen-URA3.

Depositor

Bas Teusink

Article

Botman et al Sci Rep. 2019 Feb 19;9(1):2234. doi: 10.

Insert

ymNeongreen

Expression

Yeast

Available Since

June 4, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
rbcLS-pMAL-2px

Plasmid

#41621

Depositor

Ichiro Matsumura

Article

Parikh et al Protein Eng Des Sel. 2006 Mar;19(3):113-

Insert

rbcL/rbcS

Expression

Bacterial

Promoter

Ptac

Available Since

Nov. 29, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge
pREDusk-StrR-MCS

Plasmid

#188972

Purpose

For red light-repressed bacterial expression of gene of interest (StrR)

Depositor

Heikki Takala

Article

Multamaki et al ACS Synth Biol. 2022 Aug 23. doi: 10.102

Type

Empty backbone

Expression

Bacterial

Available Since

Sept. 26, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
131-2a_LC

Plasmid

#215331

Purpose

Expression monoclonal antibody 131-2a LC

Depositor

Joost Snijder

Article

Peng et al bioRxiv 2025.01.04.631317

Insert

131-2a_LC

Expression

Mammalian

Promoter

CMV

Available Since

March 20, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
131-2a_HC

Plasmid

#214965

Purpose

Expression monoclonal antibody 131-2a

Depositor

Joost Snijder

Article

Peng et al bioRxiv 2025.01.04.631317

Insert

131-2a_HC

Tags

his8

Expression

Mammalian

Promoter

CMV

Available Since

March 20, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pLV_ADRB2-mTurquoise2-iLID

Plasmid

#161002

Purpose

Mammalian expression of mTurquoise2-tagged iLID anchored to the plasma membrane via N-terminal fusion to ADRB2 for slow diffusion

Depositor

Sean R Collins

Article

Natwick et al ACS Synth Biol. 2021 May 21;10(5):1009-1

Insert

ADRB2-mTurquoise2-iLID

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Promoter

EF1a

Available Since

Nov. 16, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
MET-HYGRO

Plasmid

#218113

Purpose

Integration backbone for the MET15 integration site of S. cerevisiae. Includes a HygromycinR gene and a sfGFP drop-out.

Depositor

Carl Denard

Article

Martinusen et al Protein Eng Des Sel. 2024 Jan 29;37:gzae

Type

Empty backbone

Expression

Bacterial and Yeast

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pMOD_C'5014

Plasmid

#127219

Purpose

Module C' with Pnos::WUS2

Depositor

Daniel Voytas

Article

Maher et al Nat Biotechnol. 2019 Dec 16. pii: 10.103

Insert

WUS2

Expression

Plant

Mutation

codon optimized CDSs

Available Since

Dec. 17, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pEX128·gRNA

Plasmid

#187600

Purpose

Template for amplification of gRNA with Cas6 recognition site

Depositor

Pablo Ivan Nikel

Article

Volke et al Nat Commun. 2022 May 31;13(1):3026. doi:

Insert

gRNA template

Promoter

no promoter

Available Since

July 22, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pDRF1-GW ymScarletI-T2A-mTurquoise2

Plasmid

#118441

Purpose

Produces equimolar expression of yeast codon-optimized mScarletI and mTq2

Depositor

Bas Teusink

Article

Botman et al Sci Rep. 2019 Feb 19;9(1):2234. doi: 10.

Insert

ymScarletI-T2A-mTurquoise2

Expression

Yeast

Available Since

Feb. 26, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pCTCON2-BXG

Plasmid

#158127

Purpose

Dual fluorescent protein reporter (BFP-GFP) for ncAA incorporation (TAG construct)

Depositor

James Van Deventer

Article

Potts et al Mol Syst Des Eng. 2020 Feb 1;5(2):573-58

Insert

mTagBFP

Expression

Yeast

Promoter

GAL1-10

Available Since

Jan. 12, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge

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We narrowed to 159,093 results for: Des