Addgene

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pAAV-SYN-L-sDarken

Plasmid

#184798

Purpose

Low affinity version of the Serotonin Sensor sDarken under the control of a hSyn Promoter.

Depositor

Olivia Masseck

Article

Kubitschke et al Nat Commun. 2022 Dec 6;13(1):7525. doi:

Insert

L-sDarken

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

hSyn

Available since

Feb. 2, 2023

Availability

Academic Institutions and Nonprofits only

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Cas9-sgRNA-A+B

Plasmid

#149370

Purpose

Expresses SpCas9 and two sgRNA targeting the lenti-CDDR reporter at two distal sites

Depositor

Tarek Abbas

Article

Eki et al Nucleic Acids Res. 2020 Oct 17. pii: 592

Inserts

Cas9
sgRNA-A
sgRNA-B
Puromycin resistance

Use

CRISPR

Tags

3xFLAG, Nucleoplasmin NLS, and SV40 NLS

Expression

Mammalian

Mutation

Promoter

CBh, PGK, and U6

Available since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pLX317-EGFP

Plasmid

#115439

Purpose

Constitutive lentiviral expression

Depositor

William Hahn

Article

Li et al Elife. 2018 Jul 30;7. pii: 37184. doi: 1

Insert

EGFP

Use

Lentiviral

Tags

V5

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

Sept. 17, 2018

Availability

Academic Institutions and Nonprofits only

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hPU.1ΔN165-pET28b

Plasmid

#199796

Purpose

Expressed human PU.1/Spi-1 ETS domain (residues 165 to 270) in E. coli

Depositor

Gregory Poon

Article

Xhani et al Sci Adv. 2020 Feb 21;6(8):eaay3178. doi:

Insert

spi1 (SPI1 Human)

Use

Tags

None

Expression

Bacterial

Mutation

Promoter

Available since

June 1, 2023

Availability

Academic Institutions and Nonprofits only

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pMYC-GFP

Plasmid

#42142

Depositor

Olufunmilayo Olopade

Article

Chen et al BMC Cancer. 2011 Jun 13;11:246. doi: 10.

Insert

c-Myc (MYC Human)

Use

Tags

GFP

Expression

Bacterial and Mammalian

Mutation

Promoter

CMV

Available since

Oct. 31, 2013

Availability

Academic Institutions and Nonprofits only

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pLenti-V5-KDM3A

Plasmid

#82331

Purpose

lentiviral expression of KDM3A

Depositor

Luke Gaughan

Article

Wade et al Nucleic Acids Res. 2015 Jan;43(1):196-20

Insert

KDM3A (KDM3A Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Mutation

two silent mutations to make it siRNA resistant

Promoter

Available since

March 14, 2017

Availability

Academic Institutions and Nonprofits only
pLenti Lifeact-iRFP670 BlastR

Plasmid

#84385

Purpose

Lentiviral expression of iRFP670-tagged Lifeact with Blasticidin selection in cells including neurons

Depositor

Ghassan Mouneimne

Article

Padilla-Rodriguez et al Nat Commun. 2018 Jul 30;9(1):2980. doi:

Insert

Lifeact

Use

Lentiviral

Tags

iRFP670

Expression

Mutation

Promoter

CMV immediate early

Available since

Jan. 5, 2017

Availability

Academic Institutions and Nonprofits only

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AAV CMV-driven AcrIIC3-scaffold (2xBsmBI sites)

Plasmid

#120301

Purpose

AAV vector for expression of AcrIIC3 (no miR binding sites, control vector)

Depositor

Dominik Niopek

Article

Hoffmann et al Nucleic Acids Res. 2019 Apr 15. pii: 545

Insert

AcrIIC3

Use

AAV and CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 23, 2019

Availability

Academic Institutions and Nonprofits only

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pEF5B-FRT-AP-Sstr3-GFP-DEST

Plasmid

#49098

Purpose

Expressing AP-Sstr3-GFP in mammalian cells, can be used for establishing Flp-In stable cell lines

Depositor

Maxence Nachury

Article

Ye et al Elife. 2013 Aug 6;2:e00654. doi: 10.7554

Insert

Sstr3 (Sstr3 Mouse)

Use

Tags

GFP and acceptor peptide (AP)

Expression

Mammalian

Mutation

Promoter

T7

Available since

Nov. 8, 2013

Availability

Academic Institutions and Nonprofits only

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pX330-gRNA

Plasmid

#158973

Purpose

A human codon-optimized SpCas9 and chimeric guide RNA (gRNA3) expression plasmid.

Depositor

Charles P. Lai

Article

Chien et al Nucleic Acids Res. 2020 Aug 14. pii: 589

Insert

gRNA3

Use

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Oct. 2, 2020

Availability

Academic Institutions and Nonprofits only

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pHAGE Flag HA ADAR2 CI

Plasmid

#237997

Purpose

Inducible lentiviral expression of ADAR2 CI

Depositor

David Pellman

Article

Tang et al Nature. 2022 Jun;606(7916):930-936. doi:

Insert

ADAR2 (ADARB1 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

E396A

Promoter

Available since

April 24, 2025

Availability

Academic Institutions and Nonprofits only

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Integrin β8-GFP

Plasmid

#205093

Purpose

Express GFP-tagged human integrin β8 in mammalian cells

Depositor

Bianxiao Cui

Article

Zhang et al Nat Cell Biol. 2023 Sep 28. doi: 10.1038

Insert

Integrin beta 8 (ITGB8 Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 19, 2023

Availability

Academic Institutions and Nonprofits only

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pEF5B-FRT-AP-Smo-YFP-DEST

Plasmid

#49099

Purpose

Expressing AP-Smo-YFP in mammalian cells, can be used for establishing Flp-In stable cell lines

Depositor

Maxence Nachury

Article

Ye et al Elife. 2013 Aug 6;2:e00654. doi: 10.7554

Insert

Smo (Smo Mouse)

Use

Tags

YFP and acceptor peptide (AP)

Expression

Mammalian

Mutation

Promoter

T7

Available since

Nov. 8, 2013

Availability

Academic Institutions and Nonprofits only

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YAP/TAZ tdTomato TEAD Binding Reporter

Plasmid

#228554

Purpose

Encodes a tdTomato fluorescent protein gene driven by a TEAD binding synthetic promoter. Used to track YAP/TAZ signaling pathway activity.

Depositor

Cole DeForest

Article

Kopyeva et al Adv Mater. 2024 Sep 6:e2404880. doi: 10.

Insert

tdTomato

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

GTIIC TEAD-Binding Responsive Element

Available since

Feb. 25, 2025

Availability

Academic Institutions and Nonprofits only

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pDule-pCNF

Plasmid

#85494

Purpose

Machinery plasmid expressing permissive pCNF synthetase and cognate amber suppressing tRNA. Incorporates azidoPhenylalanine.

Depositor

Ryan Mehl

Article

Miyake-Stoner et al Biochemistry. 2009 Jun 30;48(25):5953-62

Insert

para-cyanophenylalanine Mj synthetase

Use

Tags

None

Expression

Bacterial

Mutation

Y32L L65V F108W Q109M D158G I159P

Promoter

lpp (constitutive)

Available since

Jan. 10, 2017

Availability

Academic Institutions and Nonprofits only

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AAV2_CAG_oROS-G

Plasmid

#216115

Purpose

Encodes the genetically encoded, green fluorescent peroxide sensor oROS-G in AAV viral vectors.

Depositor

Andre Berndt

Article

Lee et al bioRxiv 2024.01.31.578117

Insert

oROS-G

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

April 24, 2024

Availability

Academic Institutions and Nonprofits only

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iNos-eGFP

Plasmid

#207251

Purpose

Reporter for expression of eGFP under control of iNos promoter

Depositor

Michelle Farkas

Article

Mas-Rosario et al Front Oncol. 2023 Apr 5;13:1151384. doi:

Insert

iNos Promoter (Nos2 Mouse)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 22, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV-PE1

Plasmid

#132774

Purpose

Prime editing in mammalian cells

Depositor

David Liu

Article

Anzalone et al Nature. 2019 Oct 21. pii: 10.1038/s41586

Insert

PE1

Use

Tags

SV40 NLS

Expression

Mammalian

Mutation

See manuscript

Promoter

CMV

Available since

Oct. 21, 2019

Availability

Academic Institutions and Nonprofits only

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pMGIB-3xFLAG-dyskerin

Plasmid

#53607

Purpose

Retroviral expression of human dyskerin

Depositor

Steven Artandi

Article

Venteicher et al Science. 2009 Jan 30;323(5914):644-8. do

Insert

dyskerin (DKC1 Human)

Use

Retroviral

Tags

3xFLAG

Expression

Mammalian

Mutation

Promoter

LTR

Available since

June 9, 2014

Availability

Academic Institutions and Nonprofits only
Integrin β5-GFP

Plasmid

#205090

Purpose

Express GFP-tagged human integrin β5 in mammalian cells

Depositor

Bianxiao Cui

Article

Zhang et al Nat Cell Biol. 2023 Sep 28. doi: 10.1038

Insert

Integrin beta 5 (ITGB5 Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 19, 2023

Availability

Academic Institutions and Nonprofits only

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