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We narrowed to 29,607 results for: Ide

Showing: 2001 - 2020 of 29607 results

  1. mClY-N1

    Plasmid
    #90457
    Purpose
    A YFP-based chloride sensor with enhanced chloride sensitivity, photostability and reduced pH interference. The sequence of mClY is EYFP-F46L/Q69K/H148Q/I152L/V163S/S175G/S205V/A206K.
    Depositor
    Joseph Santos-Sacchi
    Article
    Zhong et al PLoS One. 2014 Jun 5;9(6):e99095. doi: 1
    Insert
    mClY-N1
    Use
    Tags
    Expression
    Mammalian
    Mutation
    EYFP-F46L/Q69K/H148Q/I152L/V163S/S175G/S205V/A206…
    Promoter
    Available since
    May 19, 2017
    Availability
    Academic Institutions and Nonprofits only
    90457-plasmid-map-sequence-id-178580

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  5. CRY2-INPP5E

    Plasmid
    #79562
    Purpose
    expression of CRY2PHR fused to inositol 5-phosphatase domain of human INPP5E with mutated C-terminal CaaX-motif
    Depositor
    Olof Idevall-Hagren, Pietro De Camilli
    Article
    Idevall-Hagren et al Proc Natl Acad Sci U S A. 2012 Aug 28;10
    Insert
    INPP5E (INPP5E Human)
    Use
    Tags
    CRY2 (photolyase homology region domain of Arabid…
    Expression
    Mammalian
    Mutation
    C-terminal region, aa 214_644, C641A mutation to …
    Promoter
    CMV
    Available since
    July 11, 2016
    Availability
    Academic Institutions and Nonprofits only

  8. pCOA-Leu-FvPPT1-fsr1

    Plasmid
    #179868
    Purpose
    Constitutive expression vector for Saccharomyces cerevisiae comprising the polyketide synthase fsr1 from Fusarium vanettenii and the Fusarium verticillioides 4´-phosphopantetheinyltransferase PPT1
    Depositor
    Jens Laurids Sørensen
    Article
    Bejenari et al J Microbiol Biol Educ. 2023 Nov 27;24(3)
    Inserts
    PPT1
    fsr1
    Use
    Tags
    Expression
    Yeast
    Mutation
    Codon optimized for expression in Saccharomyces c…
    Promoter
    Yarrowia lipolytica EXP1 and Yarrowia lipolytica …
    Available since
    Nov. 29, 2023
    Availability
    Academic Institutions and Nonprofits only
    179868-plasmid-map-sequence-id-352936

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  9. mCherry-CRY2-INPP5E

    Plasmid
    #79561
    Purpose
    expression of mCherry-tagged CRY2PHR fused to inositol 5-phosphatase domain of human INPP5E with mutated C-terminal CaaX-motif
    Depositor
    Olof Idevall-Hagren, Pietro De Camilli
    Article
    Idevall-Hagren et al Proc Natl Acad Sci U S A. 2012 Aug 28;10
    Insert
    INPP5E (INPP5E Human)
    Use
    Tags
    CRY2 (photolyase homology region domain of Arabid…
    Expression
    Mammalian
    Mutation
    C-terminal region, aa 214_644, C641A mutation to …
    Promoter
    CMV
    Available since
    July 11, 2016
    Availability
    Academic Institutions and Nonprofits only

  10. mCherry-CRY2-INPP5E(D556A)

    Plasmid
    #79563
    Purpose
    expression of mCherry-tagged CRY2PHR fused to catalytically dead INPP5E
    Depositor
    Olof Idevall-Hagren, Pietro De Camilli
    Article
    Idevall-Hagren et al Proc Natl Acad Sci U S A. 2012 Aug 28;10
    Insert
    INPP5E (INPP5E Human)
    Use
    Tags
    CRY2 (photolyase homology region domain of Arabid…
    Expression
    Mammalian
    Mutation
    C-terminal region, aa 214_644, C641A mutation to …
    Promoter
    CMV
    Available since
    July 11, 2016
    Availability
    Academic Institutions and Nonprofits only

  11. pCJ217

    Plasmid
    #162685
    Purpose
    pET-21b(+) based plasmid for expression of MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1), codon optimized for expression in E. coli K12, with C-terminal His tag, incorporating two point mutations, G489C and S530C, to introduce a 6th disulfide bond (from PETase).
    Depositor
    Gregg Beckham
    Article
    Knott et al Proc Natl Acad Sci U S A. 2020 Oct 13;11
    Insert
    MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1), with mutations to introduce a 6th disulfide bond from PETase
    Use
    Tags
    His
    Expression
    Bacterial
    Mutation
    G489C, S530C; codon optimized for E. coli K12
    Promoter
    T7/lac
    Available since
    Jan. 6, 2021
    Availability
    Academic Institutions and Nonprofits only
    162685-plasmid-map-sequence-id-320886

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  13. pCJ200

    Plasmid
    #162673
    Purpose
    pET-21b(+) based plasmid for expression of MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1), codon optimized for expression in E. coli K12, with C-terminal His tag, incorporating S136C and a sequence Tyr75:Phe104 to introduce a 6th disulfide bond as in AoFaeB-2.
    Depositor
    Gregg Beckham
    Article
    Knott et al Proc Natl Acad Sci U S A. 2020 Oct 13;11
    Insert
    MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1)
    Use
    Tags
    His
    Expression
    Bacterial
    Mutation
    S136C and a sequence Y75-F104 to introduce a 6th…
    Promoter
    T7/lac
    Available since
    Jan. 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    162673-plasmid-map-sequence-id-321521

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  14. pCJ210

    Plasmid
    #162683
    Purpose
    pET-21b(+) based plasmid for expression of MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1), codon optimized for expression in E. coli K12, with C-terminal His tag, incorporating S136C, G489C, S530C, and a sequence Tyr75:Phe104 to introduce a 6th and 7th disulfide bond as in AoFaeB-2.
    Depositor
    Gregg Beckham
    Article
    Knott et al Proc Natl Acad Sci U S A. 2020 Oct 13;11
    Insert
    MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1)
    Use
    Tags
    His
    Expression
    Bacterial
    Mutation
    S136C, G489C, S530C, and a sequence Y75-F104 to i…
    Promoter
    T7/lac
    Available since
    Jan. 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    162683-plasmid-map-sequence-id-321486

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  15. pJK034

    Plasmid
    #72383
    Purpose
    Produces Bacillus subtilis glycinamide ribonucleotide synthetase (PurD)
    Depositor
    JoAnne Stubbe, T. J. Kappock
    Insert
    phosphoribosylglycinamide synthetase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    April 11, 2016
    Availability
    Academic Institutions and Nonprofits only
    72383-plasmid-map-sequence-id-136200

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  16. pET28a-RupA_T1-NHis

    Plasmid
    #109259
    Purpose
    NRPS T domain with N-terminal 6His tag
    Depositor
    Emily Balskus
    Article
    Schneider et al Tetrahedron
    Insert
    RupA_T1
    Use
    Tags
    His tag
    Expression
    Bacterial
    Mutation
    contains aa 917..1025 of WP_015523502.1
    Promoter
    T7
    Available since
    July 6, 2018
    Availability
    Academic Institutions and Nonprofits only
    109259-plasmid-map-sequence-id-211504

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  17. pET28a-RupA_R-NHis

    Plasmid
    #109257
    Purpose
    NRPS R domain with N-terminal 6His tag
    Depositor
    Emily Balskus
    Article
    Schneider et al Tetrahedron
    Insert
    RupA_R
    Use
    Tags
    His tag
    Expression
    Bacterial
    Mutation
    contains aa 2038..2443 of WP_015523502.1
    Promoter
    T7
    Available since
    July 6, 2018
    Availability
    Academic Institutions and Nonprofits only
    109257-plasmid-map-sequence-id-211503

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  18. pJQ210SK

    Plasmid
    #78499
    Purpose
    Cosmid version of suicide vector pJQ200SK. Suicide vector
    Depositor
    Michael Hynes
    Article
    Quandt et al Gene. 1993 May 15;127(1):15-21.
    Type
    Empty backbone
    Use
    Suicide vector
    Tags
    none
    Expression
    Mutation
    Promoter
    non
    Available since
    Aug. 1, 2016
    Availability
    Academic Institutions and Nonprofits only

  19. pET29b-RupA_T2-R-CHis

    Plasmid
    #109258
    Purpose
    NRPS di-domain containing TR domains with C-terminal 6His tag
    Depositor
    Emily Balskus
    Article
    Schneider et al Tetrahedron
    Insert
    RupA_T2-R
    Use
    Tags
    His tag and S-Tag
    Expression
    Bacterial
    Mutation
    contains aa 1939..2443 of WP_015523502.1
    Promoter
    T7
    Available since
    July 6, 2018
    Availability
    Academic Institutions and Nonprofits only
    109258-plasmid-map-sequence-id-211511

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  20. pET29b-RupA_C2-A2-T2-R-CHis

    Plasmid
    #109256
    Purpose
    NRPS module containing CATR domains with C-terminal 6His tag
    Depositor
    Emily Balskus
    Article
    Schneider et al Tetrahedron
    Insert
    RupA_C2-A2-T2-R
    Use
    Tags
    His tag and S-Tag
    Expression
    Bacterial
    Mutation
    contains aa 1023..2443 of WP_015523502.1
    Promoter
    T7
    Available since
    July 6, 2018
    Availability
    Academic Institutions and Nonprofits only
    109256-plasmid-map-sequence-id-214798

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Showing: 2001 - 2020 of 29607 results