We narrowed to 12,214 results for: LEA

Showing: 2001 - 2020 of 12214 results

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ND4.2-Left TALE-G1397-N-DddA11-mCherry

Plasmid

#179682

Purpose

Expression of base editor in mammalian cells and for cell sorting

Depositor

David Liu

Publication

Mok et al Nat Biotechnol. 2022 Sep;40(9):1378-1387

Insert

COX8a MTS_3xFLAG_ND4.2 Left TALE_2aa_DddA G1397-N (S1330I A1341V N1342S E1370K T1380I)_1xUGI_P2A_mCherry_ATP5B 3'UTR

Expression

Mammalian

Mutation

S1330I A1341V N1342S E1370K T1380I

Available since

April 4, 2022

Availability

Academic Institutions and Nonprofits only

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pMRXIP-GFP-hATG2A

Plasmid

#114462

Purpose

Expresses human ATG2A with GFP in mammalian cells

Depositor

Noboru Mizushima

Publication

Tamura et al FEBS Lett. 2017 Dec;591(23):3819-3830. d

Insert

human ATG2A (ATG2A Human)

Use

Retroviral

Tags

GFP

Expression

Mammalian

Mutation

P656R (please see depositors comment below)

Promoter

CMV

Available since

Sept. 20, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pBABE-Flag-Cdk9-IRES-eGFP

Plasmid

#28096

Depositor

Andrew Rice

Publication

Dow et al J Cell Physiol. 2010 Jul . 224(1):84-93.

Insert

Cdk9 (CDK9 Human)

Use

Retroviral

Tags

FLAG

Expression

Mammalian

Available since

April 15, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles
pAAV-hSyn-GRAB_r5-HT1.0

Plasmid

#208719

Purpose

Expresses the red 5-HT sensor GRAB_r5-HT1.0 in neurons

Depositor

Yulong Li

Publication

Deng et al Nat Methods. 2024 Apr;21(4):692-702. doi

Insert

Red fluorescent 5-HT sensor GRAB_r5-HT1.0

Use

AAV

Promoter

hSyn

Available since

Oct. 24, 2023

Availability

Academic Institutions and Nonprofits only

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HOXA10-MSCV

Plasmid

#20980

Depositor

Corey Largman

Publication

Thorsteinsdottir et al Mol Cell Biol. 1997 Jan . 17(1):495-505.

Insert

HOXA10 (HOXA10 Human)

Use

Retroviral

Expression

Mammalian

Available since

Aug. 14, 2009

Availability

Academic Institutions and Nonprofits only
pcDNA3.1-Beta1

Plasmid

#140987

Purpose

Encodes a Gbeta subunit (GNB1) as an optimal component of a BRET2 biosensor for studying heterotrimeric G protein dissociation

Depositor

Bryan Roth

Publication

Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

GBeta1 (GNB1 Human)

Expression

Mammalian

Mutation

Contains an N-terminal human rhinovirus (HRV) 3C …

Promoter

CMV

Available since

June 26, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pFastbac1-GST-hUSP7

Plasmid

#63573

Purpose

Expression of synthetic human USP7 tagged with GST

Depositor

Titia Sixma

Publication

Faesen et al Mol Cell. 2011 Oct 7;44(1):147-59. doi:

Insert

USP7 (USP7 Human, Synthetic)

Tags

GST

Expression

Insect

Mutation

Synthetic based on human protein sequence (please…

Promoter

Polyhedrin

Available since

April 17, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pCMV5 Flag-NLS-Smad2

Plasmid

#14931

Depositor

Joan Massague

Publication

Lo et al Nat Cell Biol. 1999 Dec . 1(8):472-8.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

NLS-Smad2 (SMAD2 Human)

Tags

Flag

Expression

Mammalian

Mutation

full-length Smad2 is fused to a nuclear-localizat…

Available since

May 17, 2007

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 FLIPE-1m

Plasmid

#13544

Depositor

Wolf Frommer

Publication

Okumoto et al Proc Natl Acad Sci U S A. 2005 Jun 14. 1

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

FLIP-E A207W

Tags

ECFP and Venus

Expression

Mammalian

Mutation

A207W in binding protein

Available since

Dec. 15, 2006

Availability

Academic Institutions and Nonprofits only

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OCT4 GFP puro Donor 2

Plasmid

#22210

Depositor

Rudolf Jaenisch

Publication

Hockemeyer et al Nat Biotechnol. 2009 Sep;27(9):851-7. do

Insert

SA-OCT-GFP-2APuro-PA (POU5F1 Human)

Available since

Oct. 14, 2009

Availability

Academic Institutions and Nonprofits only

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SpyCatcher002-oTet

Plasmid

#124663

Purpose

Expresses SpyCatcher002-oTet for tetramerization of SpyTag-proteins via tetrameric coiled-coil

Depositor

Mark Howarth

Publication

Khairil Anuar et al Nat Commun. 2019 Apr 15;10(1):1734. doi:

Insert

SpyCatcher002-oTet

Tags

C-tag, His6, and TEV cleavage site

Expression

Bacterial

Promoter

T7

Available since

May 6, 2019

Availability

Academic Institutions and Nonprofits only

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pJRT.Agro.CLCrVB1.3

Plasmid

#37974

Depositor

Niki Robertson

Publication

Tuttle et al Plant Methods. 2012 Aug 1;8(1):27. doi:

Insert

pJRTCLCrVB1.3

Use

Agrobacterium binary

Available since

Oct. 15, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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GFP-JNK2

Plasmid

#86831

Purpose

mammalian expression of GFP-JNK2

Depositor

Rony Seger

Publication

Zehorai et al Mol Cell Biol. 2014 Jan;34(2):259-70. do

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

JNK2 (MAPK9 Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available since

Feb. 9, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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tetO-GFPnls-mPGK-rtTA-IRES-Puro

Plasmid

#69545

Purpose

Tet-On system expressing GFP tagged with 2x nuclear localization signals.

Depositor

Charles Gersbach

Insert

eGFP

Use

Lentiviral

Tags

2x nuclear localization sequence and T2A "sk…

Available since

April 12, 2019

Availability

Academic Institutions and Nonprofits only

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pET Biotin His6 mCitrine LIC cloning vector (H6-mCitrine)

Plasmid

#29724

Depositor

Scott Gradia

Type

Empty backbone

Tags

6xHis, AviTag (biotin), TEV cleavage site, and mC…

Expression

Bacterial

Available since

June 7, 2011

Availability

Academic Institutions and Nonprofits only

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pDEST26 Ago2

Plasmid

#59904

Purpose

Expresses 6xHis-Ago2 (N-termi tag) in mammalian cells

Depositor

Carl Novina

Publication

Horman et al Mol Cell. 2013 May 9;50(3):356-67. doi:

Insert

Argonaute 2 (AGO2 Human)

Tags

6xHis (N-termi tag)

Expression

Mammalian

Promoter

CMV

Available since

May 26, 2015

Availability

Academic Institutions and Nonprofits only

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Nrf1-Myc pcDNA3.1(+)-C-Myc

Plasmid

#169125

Purpose

expression of protein

Depositor

Barbara Dirac-Svejstrup

Publication

Dirac-Svejstrup et al Mol Cell. 2020 Jul 16;79(2):332-341.e7.

Insert

hNrf1 (NFE2L1 Human)

Expression

Mammalian

Mutation

wt

Available since

May 13, 2021

Availability

Academic Institutions and Nonprofits only

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pACEC3GH (Hyg)

Plasmid

#110084

Purpose

Replicative, acetamide-based expression vector containing a C-terminal 3C cleavage site and a GPF-fusion with a His10-tag. Suitable for high-level expression levels amenable for large scale purification of proteins.

Depositor

Markus Seeger

Publication

Arnold et al Sci Rep. 2018 Jun 22;8(1):9539. doi: 10.

Type

Empty backbone

Tags

3C cleavage site - GFP - His10 Tag

Expression

Bacterial

Available since

June 5, 2018

Availability

Academic Institutions and Nonprofits only

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pEJS887_pTetR-P2A-BFPnls/sgAlpha

Plasmid

#108651

Purpose

Alpha satellite repeats targeting Spy sgRNA under U6 promoter

Depositor

Erik Sontheimer

Publication

Gao et al Nat Methods. 2018 Jun;15(6):433-436. doi

Inserts

Alpha satellite repeats targeting sgRNA
TetR-P2A-BFP

Use

CRISPR and Lentiviral

Tags

No

Expression

Mammalian

Promoter

U6 and hPGK

Available since

May 8, 2018

Availability

Academic Institutions and Nonprofits only

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pHJ321

Plasmid

#20676

Depositor

Stephen Shaw

Publication

Hao et al J Cell Biol. 2009 Feb 9. 184(3):451-62.

Insert

Moesin (MSN Human)

Tags

GFP

Expression

Mammalian

Mutation

Changed Thr 558 to Alanine

Available since

March 31, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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