We narrowed to 29,769 results for: LIS

1. pC1300_pUB10_pcoCASphi_E9t_MCS_version1

   Plasmid

   #197937

   Purpose

   T-DNA binary vector to express pcoCasphi driven by UBQ10 gene promoter, with SV40 NLS at the N-terminal and nucleoplasmin NLS at the C-terminal.

   Depositor

   Steven Jacobsen

   Article

   Li et al Proc Natl Acad Sci U S A. 2023 Jan 24;12

   Insert

   pcoCasphi-2
   

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   Promoter

   pUBQ10

   Available since

   March 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pNL(CMV)Rep78-III/CMV/WPREΔU3

   Plasmid

   #190726

   Purpose

   Expression of AAV2 Rep78-III in mammalian cells

   Depositor

   Jakob Reiser

   Article

   Li et al Hum Gene Ther Methods. 2018 Jun;29(3):13

   Insert

   AAV2 Rep78-III
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV-IE

   Available since

   Nov. 10, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCASCADE-xylA

   Plasmid

   #158611

   Purpose

   Low phosphate inducible gRNA silencing the xylA promoter

   Depositor

   Michael Lynch

   Article

   Li et al Metab Eng. 2021 Jan 15. pii: S1096-7176(

   Insert

   xylA guide array
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   E . coli ugpB gene promoter, low phosphate induct…

   Available since

   Jan. 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. DDX41_p.L237F_p.P238T_EGFP

   Plasmid

   #175845

   Purpose

   Expresses DDX41 with mutation

   Depositor

   Evan Braunstein

   Article

   Li et al Haematologica. 2016 Jun;101(6):e228-31.

   Insert

   DDX41 (DDX41 Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   c.711G>T, p.L237F and c.712C>A, p.P238T

   Promoter

   CMV

   Available since

   Oct. 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pGST-POLA1-N0

   Plasmid

   #160983

   Purpose

   Express a fragment of mouse Pola1 protein

   Depositor

   Zhiguo Zhang

   Article

   Li et al Sci Adv. 2020 Aug 26;6(35):eabb5820. doi

   Insert

   POLA1 (Pola1 Mouse)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Nov. 24, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pGST-POLA1-N2

   Plasmid

   #160985

   Purpose

   Express a fragment of mouse Pola1 protein

   Depositor

   Zhiguo Zhang

   Article

   Li et al Sci Adv. 2020 Aug 26;6(35):eabb5820. doi

   Insert

   POLA1 (Pola1 Mouse)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Nov. 24, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pGST-pol1-N2-2A2

   Plasmid

   #160991

   Purpose

   Express a fragment of yeast pol1 protein

   Depositor

   Zhiguo Zhang

   Article

   Li et al Sci Adv. 2020 Aug 26;6(35):eabb5820. doi

   Insert

   pol1
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   F58A/D62A

   Promoter

   Available since

   Nov. 24, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pGST-POLA1-N1

   Plasmid

   #160984

   Purpose

   Express a fragment of mouse Pola1 protein

   Depositor

   Zhiguo Zhang

   Article

   Li et al Sci Adv. 2020 Aug 26;6(35):eabb5820. doi

   Insert

   POLA1 (Pola1 Mouse)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Nov. 24, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pGST-pol1-N1-2A2

   Plasmid

   #160990

   Purpose

   Express a fragment of yeast pol1 protein

   Depositor

   Zhiguo Zhang

   Article

   Li et al Sci Adv. 2020 Aug 26;6(35):eabb5820. doi

   Insert

   pol1
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   F58A/D62A

   Promoter

   Available since

   Nov. 20, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pHR_TRE3G-p300-dCas9-P2A-mCherry

    Plasmid

    #138456

    Purpose

    2nd Generation Lentiviral vector. Expresses an N-terminal p300-dCas9 fusion protein and mCherry

    Depositor

    Jian Xu

    Article

    Li et al Nat Commun. 2020 Jan 24;11(1):485. doi:

    Insert

    p300 core
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE3G

    Available since

    Jan. 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pREDCas9

    Plasmid

    #71541

    Purpose

    Constitutive expression of Cas9, inducible expression of the Red recombineering system, and inducible expression of the plasmid curing system for CRISPR mediated genome editing of E. coli.

    Depositor

    Tao Chen

    Article

    Li et al Metab Eng. 2015 Sep;31:13-21. doi: 10.10

    Inserts

    Cas9
    lambda red genes
    plasmid curing system
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    pBAD

    Available since

    July 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. AAEL010097-Cas9

    Plasmid

    #100707

    Purpose

    The promoter of AAEL010097 drive expression of Streptococcus pyogenes Cas9 gene in Aedes aegypti

    Depositor

    Omar Akbari

    Article

    Li et al Proc Natl Acad Sci U S A. 2017 Nov 14. p

    Inserts

    AAEL010097-hspcas9-GFP
    Opie2-dsRed
    

    Use

    CRISPR

    Tags

    GFP and dsRED

    Expression

    Insect

    Mutation

    Promoter

    AAEL010097 and Opie2

    Available since

    Oct. 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. gRNA-CasRx_SD1-pSV40-TagBFP

    Plasmid

    #221004

    Purpose

    Transiently express CasRx DR30 repeat with gRNA spacer. Contains SD1 mutation to enhance efficiency. SV40-TagBFP cassette to monitor transfection efficiency. Use BbsI with overhangs Fw-AAAC, Rv-AAAA.

    Depositor

    Benjamin Blencowe, Mikko Taipale

    Article

    Li et al Mol Cell. 2024 Jul 11;84(13):2573-2589.e

    Insert

    CasRx 30nt processed direct repeat with SD1 mutation
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    DR1 A>T

    Promoter

    U6

    Available since

    June 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pGL3-EF1a-i53

    Plasmid

    #216243

    Purpose

    Express i53 in mammalian cells as a HDR enhancer

    Depositor

    Elina Ikonen

    Article

    Li et al Genome Biol. 2024 Feb 26;25(1):58. doi:

    Insert

    i53
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    March 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. AAEL005635-Cas9

    Plasmid

    #100608

    Purpose

    The promoter of AAEL005635 drive expression of Streptococcus pyogenes Cas9 gene in Aedes aegypti

    Depositor

    Omar Akbari

    Article

    Li et al Proc Natl Acad Sci U S A. 2017 Nov 14. p

    Inserts

    AAEL005635-hspcas9-GFP
    Opie2-dsRed
    

    Use

    CRISPR

    Tags

    GFP and dsRED

    Expression

    Insect

    Mutation

    Promoter

    AAEL005635 and Opie2

    Available since

    Oct. 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pET3a-OSF-CypA

    Plasmid

    #79039

    Purpose

    Bacterial vector for cloning and propagation in DH5alpha

    Depositor

    Wesley Sundquist

    Article

    Li et al Elife. 2016 Jun 2;5. pii: e16269. doi: 1

    Insert

    OneSTrEP-FLAG- Cyclophilin A (PPIA Human)
    

    Use

    Tags

    OneSTrEP-FLAG

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Jan. 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA3 FlipGFP(Mpro) T2A mCherry

    Plasmid

    #163078

    Purpose

    Expresses FlipGFP-based activity reporter of SARS-CoV-2 main protease Mpro and also mCherry

    Depositor

    Xiaokun Shu

    Article

    Li et al PLoS Pathog. 2021 Sep 3;17(9):e1009898.

    Insert

    FlipGFP-based Mpro activity reporter and mCherry
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. MIB2-FLAG

    Plasmid

    #37117

    Depositor

    Martin Dorf

    Article

    Li et al Immunity. 2011 Sep 23;35(3):426-40. Epub

    Insert

    MIB2, mindbomb E3 ubiquitin protein ligase 2 (MIB2 Human)
    

    Use

    Tags

    3x FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 3, 2012

    Availability

    Academic Institutions and Nonprofits only

19. HA-VHL wt-pBabe-puro

    Plasmid

    #19234

    Depositor

    William Kaelin

    Article

    Li et al Mol Cell Biol. 2007 Aug . 27(15):5381-92

    Insert

    von Hippel-Lindau tumor suppressor (VHL) (VHL Human)
    

    Use

    Retroviral

    Tags

    HA-tag

    Expression

    Mammalian

    Mutation

    wt

    Promoter

    Available since

    Oct. 1, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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20. CMV-mCherry-3’UTR of β-actin-(F30-2xdBroccoli)6

    Plasmid

    #190162

    Purpose

    Expresses 24 Broccoli-tagged beta-actin mRNA in mammalian cells

    Depositor

    Samie Jaffrey

    Article

    Li et al Angew Chem Int Ed Engl. 2020 Mar 9;59(11

    Insert

    mcherry_(3'UTR, No Actin)_24Broccoli
    

    Use

    Tags

    24×Broccoli

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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