We narrowed to 4,241 results for: pes;

1. pAGT9050

   Plasmid

   #219429

   Purpose

   PapE Exonuclease fused to linker 4LF2 as N-terminal tag module (AATG_PapE-4*LF2_AGGT)

   Depositor

   Tom Schreiber

   Article

   Schreiber et al Mol Plant. 2024 Mar 22:S1674-2052(24)000

   Insert

   AATG_PapE-4xLF2_AGGT
   

   Use

   Moclo compatible level 0 module

   Available Since

   June 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAV-DIO-LACTB-dAPEX2

   Plasmid

   #117180

   Purpose

   Plasmid name in publication: pAAV-DIO-IMS-dAPEX2. Peroxidase reporter for multiplexed electron microscopy labeling

   Depositor

   David Ginty

   Article

   Zhang et al Nat Neurosci. 2019 Mar 18. pii: 10.1038/

   Insert

   LACTB-dAPEX2
   

   Use

   AAV and Cre/Lox

   Expression

   Mammalian

   Mutation

   W41F and A134P on soybean APX

   Promoter

   EF1a

   Available Since

   March 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pB[vasa-Cas9 PUb-ZsGreen]

   Plasmid

   #169012

   Purpose

   For germline transformation. Expresses Cas9 in germ cells and ZsGreen marker in all cells.

   Depositor

   Max Scott

   Article

   Kandul et al CRISPR J. 2021 Oct;4(5):739-751. doi: 10

   Insert

   Cas9
   

   Use

   Piggybac

   Expression

   Insect

   Promoter

   Drosophila melanogaster vasa

   Available Since

   April 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pDD2148

   Plasmid

   #153968

   Purpose

   pCMV6-Entry vector encoding human IRAK1 protein 196F

   Depositor

   Dirk Dittmer

   Article

   Seltzer et al J Virol. 2020 May 4;94(10). pii: JVI.021

   Insert

   IRAK1 (IRAK1 Human)
   

   Tags

   Myc-DDK

   Expression

   Mammalian

   Available Since

   June 29, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pH3R-mCherry-N1

   Plasmid

   #84327

   Purpose

   Histamine-3 receptor tagged with mCherry

   Depositor

   Dorus Gadella

   Article

   van Unen et al Mol Pharmacol. 2016 Sep;90(3):162-76. do

   Insert

   H3R-mCherry (HRH3 Human)
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Dec. 9, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAV-DIO-IGK-dAPEX2-KDEL

   Plasmid

   #117183

   Purpose

   Plasmid name in publication: pAAV-DIO-ER-dAPEX2. Peroxidase reporter for multiplexed electron microscopy labeling

   Depositor

   David Ginty

   Article

   Zhang et al Nat Neurosci. 2019 Mar 18. pii: 10.1038/

   Insert

   IGK-dAPEX2-KDEL
   

   Use

   AAV and Cre/Lox

   Expression

   Mammalian

   Mutation

   W41F and A134P on soybean APX

   Promoter

   EF1a

   Available Since

   March 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV-CaMKII-Ace-mNeon2

   Plasmid

   #195526

   Purpose

   Green fluorescent, negative response-polarity voltage indicator under the control of CaMKII promoter

   Depositor

   Vincent Pieribone

   Article

   Kannan et al Science. 2022 Nov 4;378(6619):eabm8797.

   Insert

   Ace-mNeon2
   

   Use

   AAV

   Expression

   Mammalian

   Mutation

   Ace-mNeon 81S; SY linker

   Promoter

   CaMKII

   Available Since

   Jan. 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. U6-pegRNA-H1-nick sgRNA-mCherry

   Plasmid

   #176901

   Purpose

   Expresses prime editing guide RNA and guide RNA in mammalian cells

   Depositor

   Hyongbum Kim

   Article

   Jang et al Nat Biomed Eng. 2021 Aug 26. pii: 10.103

   Inserts

   mCherry
   gRNA scaffold
   gRNA scaffold
   

   Use

   AAV

   Expression

   Mammalian

   Promoter

   CAG, H1, and U6

   Available Since

   Dec. 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pET22b-T5-sfGFP*-XcP4H-MH4R

   Plasmid

   #178043

   Purpose

   Express green fluorescent protein with TAG at 151 position and enzymes to biosynthesize 5HTP

   Depositor

   Han Xiao

   Article

   Chen et al Chem. 2020 Oct 8;6(10):2717-2727. doi: 1

   Inserts

   superfolder green fluorescent protein with TAG at 151 position
   XcP4H with W179F mutation
   dihydropterine reductase from human
   pterin-4a-carbinolamine dehydratase from human
   

   Tags

   His tag

   Expression

   Bacterial

   Available Since

   April 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3-HCMVgL-FLAG (TB40/E)

    Plasmid

    #136449

    Purpose

    Mammalian expression plasmid for HCMV glycoprotein L (TB40/E strain) with FLAG tag

    Depositor

    Erik Procko

    Article

    Park et al PLoS Pathog. 2020 Jun 19;16(6):e1008647.

    Insert

    glycoprotein L (UL115 Human betaherpesvirus 5)
    

    Tags

    Short SGGSG linker and FLAG tag

    Expression

    Mammalian

    Mutation

    Codon-optimized for human cell expression.

    Promoter

    CMV

    Available Since

    July 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pTXB1-TP3

    Plasmid

    #160086

    Purpose

    Bacterial expression of Tn5-APEX2 fusion protein with linker AEAAAKEAAAKA

    Depositor

    Pier Pandolfi

    Article

    Lee et al Nat Methods. 2021 Mar;18(3):293-302. doi

    Insert

    Tn5 transposase/APEX2 peroxidase fusion protein
    

    Tags

    FLAG and Mxe intein - Chitin-binding domain

    Expression

    Bacterial

    Available Since

    Nov. 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pTXB1-TP5

    Plasmid

    #160088

    Purpose

    Bacterial expression of Tn5-APEX2 fusion protein with linker GSGAGA

    Depositor

    Pier Pandolfi

    Article

    Lee et al Nat Methods. 2021 Mar;18(3):293-302. doi

    Insert

    Tn5 transposase/APEX2 peroxidase fusion protein
    

    Tags

    FLAG and Mxe intein - Chitin-binding domain

    Expression

    Bacterial

    Available Since

    Nov. 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pdCas12a(RuvC)

    Plasmid

    #186447

    Purpose

    Encodes catalytically inactive Cas12a (D917A, E1006A) from F. novicida (type V-A)

    Depositor

    John van der Oost

    Article

    Wu et al CRISPR J. 2022 Aug;5(4):536-547. doi: 10

    Insert

    dCas12a
    

    Use

    CRISPR

    Mutation

    D917A, E1006A

    Promoter

    T7

    Available Since

    March 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pEJS887_pTetR-P2A-BFPnls/sgAlpha

    Plasmid

    #108651

    Purpose

    Alpha satellite repeats targeting Spy sgRNA under U6 promoter

    Depositor

    Erik Sontheimer

    Article

    Gao et al Nat Methods. 2018 Jun;15(6):433-436. doi

    Inserts

    Alpha satellite repeats targeting sgRNA
    TetR-P2A-BFP
    

    Use

    CRISPR and Lentiviral

    Tags

    No

    Expression

    Mammalian

    Promoter

    U6 and hPGK

    Available Since

    May 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAAV-CAG-DIO-VARNAM2-Kv2.1PR

    Plasmid

    #195533

    Purpose

    Cre-dependent expression of a red fluorescent, negative response-polarity voltage indicator; soma-targeted

    Depositor

    Vincent Pieribone

    Article

    Kannan et al Science. 2022 Nov 4;378(6619):eabm8797.

    Insert

    VARNAM2-Kv2.1 proximal restriction sequence
    

    Use

    AAV and Cre/Lox

    Expression

    Mammalian

    Mutation

    VARNAM SI linker

    Promoter

    CAG

    Available Since

    Jan. 31, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. mCerulean3-BclXL-pEGFP-C1

    Plasmid

    #177408

    Purpose

    To express mCerulean3 fused to the N-terminus of the Bcl-2 family protein, Bcl-XL.

    Depositor

    David Andrews

    Article

    Liu et al Elife. 2019 Mar 12;8. pii: 37689. doi: 1

    Insert

    Bcl-XL
    

    Tags

    mCerulean3

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pdCas12b(RuvC)

    Plasmid

    #186450

    Purpose

    Encodes catalytically inactive Cas12b (E848A, D977A) from A. acidoterrestris (type V-B)

    Depositor

    John van der Oost

    Article

    Wu et al CRISPR J. 2022 Aug;5(4):536-547. doi: 10

    Insert

    dCas12b
    

    Use

    CRISPR

    Mutation

    E848A, D977A

    Promoter

    T7

    Available Since

    March 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAAV-IGK-dAPEX2-KDEL

    Plasmid

    #117182

    Purpose

    Plasmid name in publication: pAAV-ER-dAPEX2. Peroxidase reporter for multiplexed electron microscopy labeling

    Depositor

    David Ginty

    Article

    Zhang et al Nat Neurosci. 2019 Mar 18. pii: 10.1038/

    Insert

    IGK-dAPEX2-KDEL
    

    Use

    AAV

    Expression

    Mammalian

    Mutation

    W41F and A134P on soybean APX

    Promoter

    EF1a

    Available Since

    March 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pB[nos-Cas9 PUb-ZsGreen]

    Plasmid

    #169011

    Purpose

    For germline transformation. Expresses Cas9 in germ cells and ZsGreen marker in all cells.

    Depositor

    Max Scott

    Article

    Kandul et al CRISPR J. 2021 Oct;4(5):739-751. doi: 10

    Insert

    Cas9
    

    Use

    Piggybac

    Expression

    Insect

    Promoter

    Drosophila melanogaster nanos

    Available Since

    April 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. Fireworks GFP[PTC-] (AVA2598)

    Plasmid

    #85445

    Purpose

    Expresses TEV protease and tandemly-repeated GFP fluorescent proteins to amplify the fluorescence signal produced by a single transcription unit of a beta-globin reporter.

    Depositor

    Joan Steitz

    Article

    Alexandrov et al Mol Cell. 2017 Jan 5;65(1):191-201. doi:

    Insert

    tDNA1 EF1alfa-5xEGFP-TEVprotease-PEST-beta-globin(dI1)-BGH polyA tDNA2 FRT-PEST-TEVsite-PuromycinR
    

    Use

    Minimal backbone fragment for low-copy bacterial …

    Expression

    Mammalian

    Available Since

    Feb. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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