161,273 results

1. G15-CASE

   Plasmid

   #168126

   Purpose

   Encodes a BRET-based activity sensor for the heterotrimeric G protein G15. Composed of the subunits G alpha 15 (GNA15) tagged with NanoLuciferase, G beta 3 (GNB3) and Venus-tagged G gamma 9 (GNG9).

   Depositor

   Gunnar Schulte

   Article

   Schihada et al Sci Signal. 2021 Sep 7;14(699):eabf1653.

   Insert

   GNA15, GNB3, GNG9 (GNA15, GNB3, GNGT2 Human)
   

   Use

   Luciferase

   Tags

   cpVenus on GNG9

   Expression

   Mammalian

   Mutation

   NLuc is inserted at E244/N245 within GNA15

   Promoter

   Available since

   July 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3-TRIF-CFP

   Plasmid

   #13644

   Depositor

   Doug Golenbock

   Insert

   TRIF (TICAM1 Human)
   

   Use

   Tags

   CFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 12, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCDNA6A-EGFR WT

   Plasmid

   #42665

   Depositor

   Mien-Chie Hung

   Article

   Hsu et al J Biol Chem. 2007 Apr 6;282(14):10432-40

   Insert

   EGFR (EGFR Human)
   

   Use

   Tags

   6xHis and Myc

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 17, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3-SARS-CoV-2-S-RBD-Fc

   Plasmid

   #141183

   Purpose

   Mammalian expression plasmid for RBD of SARS-CoV-2 protein S (spike) fused with IgG1-Fc

   Depositor

   Erik Procko

   Article

   Chan et al Science. 2020 Sep 4;369(6508):1261-1265.

   Insert

   Secreted receptor binding domain (RBD; a.a. 333-529) of S protein from SARS-CoV-2 fused to Fc of IgG1 (S SARS coronavirus 2)
   

   Use

   Tags

   Fc region of IgG1 (a.a. Asp-221 to Lys-447) and S…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pFG4

   Plasmid

   #87251

   Purpose

   gene for resistance to hygromycin (Hyg) inserted next to the parS segments (INT). Also known as pANCH2-Hph

   Depositor

   Kerstin Bystricky

   Article

   Saad et al PLoS Genet. 2014 Mar 13;10(3):e1004187.

   Insert

   Hyg
   

   Use

   Tags

   parS segments

   Expression

   Yeast

   Mutation

   Promoter

   TEF

   Available since

   March 8, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pZP06C

   Plasmid

   #232322

   Purpose

   pZP06C was constructed by adding the mCherry reporter gene driven by the rpsJ promoter into pZP06B (containing PrpsJ-sacB) to facilitate the screening of positive clones through red fluorescence.

   Depositor

   Chenggang Wu

   Insert

   mCherry
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   rpsJ

   Available since

   March 12, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. ampl43

   Plasmid

   #161830

   Purpose

   pRS416-dCas9-Mxi1+TetR+pRPR1(TetO)-NotI-gRNA plasmid marked with His, expressing dCas9-Mxi1 under Tef1 promoter, and a tet-inducible promoter for gRNA expression.

   Depositor

   Saeed Tavazoie

   Article

   Momen-Roknabadi et al Commun Biol. 2020 Nov 27;3(1):723. doi:

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   TEF1

   Available since

   Sept. 30, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pHR_PGK

   Plasmid

   #79120

   Purpose

   Lentiviral vector for constitutive expression of genes (PGK promoter)

   Depositor

   Wendell Lim

   Article

   Morsut et al Cell. 2016 Feb 11;164(4):780-91. doi: 10

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 26, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pHR-OptoCAR-mScarlet

   Plasmid

   #170463

   Purpose

   Expresses an optically-controllable chimeric antigen receptor that is dependent on dimerizer drug, fused to mScarlet fluorophore

   Depositor

   John James

   Article

   Harris et al Mol Syst Biol. 2021 May;17(5):e10091. do

   Insert

   OptoCAR
   

   Use

   Lentiviral

   Tags

   mScarlet

   Expression

   Mammalian

   Mutation

   Promoter

   SFFV

   Available since

   Aug. 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAAV-hSyn-mCherry-WPRE

    Plasmid

    #127860

    Purpose

    pAAV plasmid expressing mCherry under the hSyn promoter

    Depositor

    Florian Freudenberg

    Article

    Candemir et al Eur Neuropsychopharmacol. 2016 Apr;26(4)

    Insert

    mCherry
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    hSyn

    Available since

    Aug. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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