We narrowed to 159,093 results for: Des

Showing: 4041 - 4080 of 159093 results

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Ube2r1

Plasmid

#31426

Depositor

Rachel Klevit

Article

Christensen et al Nat Struct Mol Biol. 2007 Oct . 14(10):9

Insert

Ubiquitin Conjugating Enzyme (CDC34 Human)

Tags

HA

Expression

Yeast

Available Since

Oct. 27, 2011

Availability

Academic Institutions and Nonprofits only
Ube2s

Plasmid

#31417

Depositor

Rachel Klevit

Article

Christensen et al Nat Struct Mol Biol. 2007 Oct . 14(10):9

Insert

Ubiquitin Conjugating Enzyme (UBA7 Human)

Tags

HA

Expression

Yeast

Available Since

Oct. 27, 2011

Availability

Academic Institutions and Nonprofits only
pDRF1 Cyterm-ymStayGold

Plasmid

#219855

Purpose

expression of Cyterm-ymStayGoldd in budding yeast

Depositor

Bas Teusink

Article

Botman et al bioRxiv 2025.06.27.661975; doi: https://

Insert

Cyterm-ymStayGold

Expression

Yeast

Available Since

Aug. 27, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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px458_Conc2

Plasmid

#134449

Purpose

Empty concatemer vector in which 2 sgRNAs can be inserted; with Cas9 + GFP

Depositor

Daniel Stange

Article

Gaebler et al J Vis Exp. 2020 Feb 18;(156). doi: 10.37

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

March 4, 2020

Availability

Academic Institutions and Nonprofits only

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pCTCON2-BYG

Plasmid

#158126

Purpose

Dual fluorescent protein reporter (BFP-GFP) for ncAA incorporation (wild-type construct)

Depositor

James Van Deventer

Article

Potts et al Mol Syst Des Eng. 2020 Feb 1;5(2):573-58

Insert

mTagBFP

Expression

Yeast

Promoter

GAL1-10

Available Since

Jan. 12, 2021

Availability

Academic Institutions and Nonprofits only

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pSA293-CHEQ-seq

Plasmid

#174669

Purpose

Reporter plasmid for MPRA containing a SCP1 promoter, a chimeric intron and the Venus cDNA.

Depositor

Stein Aerts

Article

Mauduit et al Elife. 2021 Dec 7;10. pii: 71735. doi: 1

Type

Empty backbone

Use

Enhancer reporter

Promoter

SCP1

Available Since

Dec. 10, 2021

Availability

Academic Institutions and Nonprofits only

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px458_Conc3

Plasmid

#134450

Purpose

Empty concatemer vector in which 3 sgRNAs can be inserted; with Cas9 + GFP

Depositor

Daniel Stange

Article

Gaebler et al J Vis Exp. 2020 Feb 18;(156). doi: 10.37

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Jan. 21, 2020

Availability

Academic Institutions and Nonprofits only

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pEXPYR

Plasmid

#70030

Purpose

High yield protein secretion in Aspergillus nidulans

Depositor

Rolf Prade

Article

Segato et al Enzyme Microb Technol. 2012 Jul 15;51(2)

Type

Empty backbone

Use

Synthetic Biology

Promoter

A. niger glucoamylase promoter (glaAp)4

Available Since

Dec. 16, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO-FLAG-MORF4L1(iso2)

Plasmid

#86874

Purpose

To express MORF4L1 (iso2) with a FLAG tag at N-terminus.

Depositor

Yihong Ye

Article

Xu et al Cell Discov. 2016 Nov 8;2:16040. eCollec

Insert

MORF4L1 (MORF4L1 Human)

Tags

FLAG

Expression

Mammalian

Mutation

none

Promoter

CMV

Available Since

April 5, 2017

Availability

Academic Institutions and Nonprofits only

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px459V2.0_Conc3

Plasmid

#134458

Purpose

Empty concatemer vector in which 3 sgRNAs can be inserted; with Cas9 + Puro

Depositor

Daniel Stange

Article

Gaebler et al J Vis Exp. 2020 Feb 18;(156). doi: 10.37

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Jan. 21, 2020

Availability

Academic Institutions and Nonprofits only

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BiP-mGFP P495L

Plasmid

#62232

Purpose

Mutant of hamster BiP-mGFP unable to bind clients.

Depositor

Erik Snapp

Article

Lai et al Mol Biol Cell. 2010 Jun 15;21(12):1909-2

Insert

hamster BiP-mGFP-KDEL

Tags

mGFP

Expression

Mammalian

Promoter

cmv

Available Since

March 25, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA6-HA-AML1-ETO

Plasmid

#12508

Depositor

Dong-Er Zhang

Article

Peterson et al Blood. 2007 May 15;109(10):4392-8. Epub

Insert

HA-AML1-ETO (RUNX1T1 Human)

Expression

Mammalian

Available Since

Jan. 4, 2007

Availability

Academic Institutions and Nonprofits only
pFA6a-ymTurquoise2-CaURA3

Plasmid

#118458

Purpose

Yeast tagging vector to create a gene fusion with yeast optimized mTq2 with CaUra3 selection. *An updated version of this plasmid is available: #168057 pFA6a-link-ymTq2-URA3.

Depositor

Bas Teusink

Article

Botman et al Sci Rep. 2019 Feb 19;9(1):2234. doi: 10.

Insert

ymTurquoise2

Expression

Yeast

Available Since

Feb. 26, 2019

Availability

Academic Institutions and Nonprofits only

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pVENUS-N1-POLR2G-GFP

Plasmid

#86872

Purpose

To express POLR2G (RPB7) tagged with VENUS at C terminus.

Depositor

Yihong Ye

Article

Xu et al Cell Discov. 2016 Nov 8;2:16040. eCollec

Insert

POLR2G (POLR2G Human)

Tags

VENUS

Expression

Mammalian

Mutation

none

Promoter

CMV

Available Since

April 5, 2017

Availability

Academic Institutions and Nonprofits only

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pIA1400

Plasmid

#172519

Purpose

Expression of SARS-COV-2 Nsp12 in E. coli

Depositor

Irina Artsimovitch

Article

Wang et al Nucleic Acids Res. 2021 Aug 5. pii: 6342

Insert

SARS-COV-2 Nsp12 (ORF1ab Synthetic)

Tags

His-SUMO

Expression

Bacterial

Promoter

T7

Available Since

Aug. 6, 2021

Availability

Academic Institutions and Nonprofits only

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pLV_Lyn11-mTurquoise2-iLID

Plasmid

#161000

Purpose

Mammalian expression of mTurquoise2-tagged iLID anchored to the plasma membrane via N-terminal fusion to lipidated peptide from LYN

Depositor

Sean R Collins

Article

Natwick et al ACS Synth Biol. 2021 May 21;10(5):1009-1

Insert

Lyn11-mTurquoise2-iLID

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

Nov. 16, 2020

Availability

Academic Institutions and Nonprofits only

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pGL4.11 promPDE4D3

Plasmid

#27302

Depositor

David E. Fisher

Article

Khaled et al Genes Dev. 2010 Oct 15. 24(20):2276-81.

Insert

prom PDE4D3 (PDE4D Human)

Use

Luciferase

Mutation

N/A

Available Since

Jan. 20, 2011

Availability

Academic Institutions and Nonprofits only

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pLV_mTurquoise2-iLID-CAAX

Plasmid

#160999

Purpose

Mammalian expression of mTurquoise2-tagged iLID anchored to the plasma membrane via C-terminal fusion to CAAX motif from KRAS

Depositor

Sean R Collins

Article

Natwick et al ACS Synth Biol. 2021 May 21;10(5):1009-1

Insert

mTurquoise2-iLID-CAAX

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

Nov. 16, 2020

Availability

Academic Institutions and Nonprofits only

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pET22-HT/spoR

Plasmid

#73631

Purpose

Produces Escherichia coli guanylate kinase (SpoR), N-terminal His6 tag

Depositor

T. J. Kappock, James Williamson

Article

Schultheisz et al ACS Chem Biol. 2008 Aug 15;3(8):499-511.

Insert

guanylate kinase

Tags

His6

Expression

Bacterial

Promoter

T7

Available Since

Feb. 23, 2016

Availability

Academic Institutions and Nonprofits only

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P1-M-P2

Plasmid

#87091

Purpose

This is the P1-M-P2 portion of the Bcl-2 promoter with a luciferase reporter in pGL3Basic plasmid

Depositor

Yohannes Tesfaigzi

Article

Bredow et al Gene. 2007 Dec 1;404(1-2):110-6. Epub 20

Type

Empty backbone

Expression

Mammalian

Available Since

July 19, 2017

Availability

Academic Institutions and Nonprofits only
pDRF1-GW yosfGFP-T2A-mCherry

Plasmid

#118448

Purpose

Produces equimolar expression of yeast codon-optimized sfGFP and mCherry

Depositor

Bas Teusink

Article

Botman et al Sci Rep. 2019 Feb 19;9(1):2234. doi: 10.

Insert

yosfGFP-T2A-mCherry

Expression

Yeast

Available Since

June 10, 2019

Availability

Academic Institutions and Nonprofits only

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pRS306-PTDH3-SUMO10-6SIM

Plasmid

#190248

Purpose

Expression SUMO10-SIM6 in yeast cells

Depositor

Liam Holt

Article

Sang et al Mol Cell. 2022 Oct 6;82(19):3693-3711.e1

Insert

10SUMO-6SIM

Expression

Yeast

Available Since

Nov. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pOC4 cloning template vector

Plasmid

#183423

Purpose

FlpON knock-in vector with FLAG-SpCas9 expression

Depositor

Harold MacGillavry

Article

Droogers et al eNeuro. 2022 Jul 18;9(4):ENEURO.0056-22.

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Available Since

April 25, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pAG306-GPD-AVT1 chr I

Plasmid

#41898

Purpose

Yeast expression of AVT1.

Depositor

Dan Gottschling

Article

Hughes et al Nature. 2012 Dec 13;492(7428):261-5. doi

Insert

AVT1 (AVT1 Budding Yeast)

Expression

Yeast

Promoter

GPD

Available Since

Feb. 7, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pDRF1-GW yomCherry-T2A-mTurquoise2

Plasmid

#118447

Purpose

Produces equimolar expression of yeast codon-optimized mCherry and mTq2

Depositor

Bas Teusink

Article

Botman et al Sci Rep. 2019 Feb 19;9(1):2234. doi: 10.

Insert

yomCherry-T2A-mTurquoise2

Expression

Yeast

Available Since

Feb. 26, 2019

Availability

Academic Institutions and Nonprofits only

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pGEX-mUbp43-His

Plasmid

#12458

Depositor

Dong-Er Zhang

Article

Malakhov et al J Biol Chem. 2002 Mar 22. 277(12):9976-8

Insert

UBP43 (Usp18 Mouse)

Tags

GST and His

Expression

Bacterial

Available Since

Jan. 4, 2007

Availability

Academic Institutions and Nonprofits only
pIA1364

Plasmid

#172401

Purpose

Expression of SARS-COV-2 Nsp9 (extra GS at the N-terminal) in E. coli

Depositor

Irina Artsimovitch

Article

Wang et al Nucleic Acids Res. 2021 Aug 5. pii: 6342

Insert

SARS-COV-2 Nsp9 (ORF1ab Synthetic)

Tags

His-GB1

Expression

Bacterial

Promoter

T7

Available Since

Aug. 6, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pDRF1 Cyterm-ytd8oxStayGold

Plasmid

#219853

Purpose

expression of Cyterm-ytd8oxStayGold in budding yeast

Depositor

Bas Teusink

Article

Botman et al bioRxiv 2025.06.27.661975; doi: https://

Insert

Cyterm-ytd8oxStayGold

Expression

Yeast

Available Since

Aug. 27, 2024

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pBEVY-4420-mCherry

Plasmid

#218828

Purpose

Yeast surface display of an anti-fluorescene scFv and intracellular expression of mCherry

Depositor

Yongku Cho

Article

Arbaciauskaite et al Protein Eng Des Sel. 2023 Jan 21;36:gzad

Insert

4-4-20 scFv

Expression

Yeast

Promoter

GAL1-10

Available Since

May 1, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pET23a-Mm/HsUBA52

Plasmid

#69560

Purpose

Bacterial expression; in vitro transcription/translation (T7)

Depositor

Jorge Eduardo Azevedo

Article

Grou et al Sci Rep. 2015 Aug 3;5:12836. doi: 10.103

Insert

UBA52 (UBA52 Human)

Expression

Bacterial

Promoter

T7

Available Since

Dec. 10, 2015

Availability

Academic Institutions and Nonprofits only
pFA6a-ytdTomato-CaURA3

Plasmid

#118461

Purpose

Yeast tagging vector to create a gene fusion with yeast optimized tdTomato with CaUra3 selection. *An updated version of this plasmid is available: #168054 pFA6a-link-ytdTomato-URA3.

Depositor

Bas Teusink

Article

Botman et al Sci Rep. 2019 Feb 19;9(1):2234. doi: 10.

Insert

ytdTomato

Expression

Yeast

Available Since

Feb. 26, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
Hulk-ubi-lacZ-to-Green

Plasmid

#108641

Purpose

ubiquitous promoter followed by a lacZ to GFP lineage tracing line. Cryst:venusGFP

Depositor

Nadia Mercader Huber

Article

Di Donato et al Genome Res. 2016 Mar 8.

Insert

ubi-lacZ-to-GFP

Available Since

Sept. 25, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pG1AK-PheB

Plasmid

#71738

Purpose

Modular shuttle vector for Geobacillus and E. coli

Depositor

Tom Ellis

Article

Reeve et al ACS Synth Biol. 2016 Jul 1.

Insert

PheB

Use

Synthetic Biology; Shuttle vector

Expression

Bacterial

Promoter

pRplsWT

Available Since

July 5, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pFA6a-ymVenus-CaURA3

Plasmid

#118459

Purpose

Yeast tagging vector to create a gene fusion with yeast optimized mVenus with CaUra3 selection. *An updated version of this plasmid is available: #168058 pFA6a-link-ymVenus-URA3.

Depositor

Bas Teusink

Article

Botman et al Sci Rep. 2019 Feb 19;9(1):2234. doi: 10.

Insert

ymVenus

Expression

Yeast

Available Since

Feb. 26, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pAG306-GPD-VPH2 chr I

Plasmid

#41897

Purpose

Yeast expression of VPH2.

Depositor

Dan Gottschling

Article

Hughes et al Nature. 2012 Dec 13;492(7428):261-5. doi

Insert

VPH2 (VPH2 Budding Yeast)

Expression

Yeast

Promoter

GPD

Available Since

Feb. 15, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
Nephrin

Plasmid

#186134

Purpose

For expression of recombinant nephrin in bacteria cells

Depositor

Michael Rosen

Article

Case et al Elife. 2022 Jan 20;11. pii: 72588. doi:

Insert

NPHS1 (NPHS1 Human)

Tags

His6 and MBP

Expression

Bacterial

Mutation

Amino acid residues 1174-1223, with mutations: Y1…

Promoter

Lac

Available Since

June 30, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pDRF1-GW ymNeongreen-T2A-mTurquoise2

Plasmid

#125701

Purpose

Produces equimolar expression of yeast codon optimized mNeongreen and mTurquoise2

Depositor

Bas Teusink

Article

Botman et al Sci Rep. 2019 Feb 19;9(1):2234. doi: 10.

Insert

ymNeongreen-T2A-mTurquoise

Expression

Yeast

Available Since

June 10, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pCRII-MBII85 probe

Plasmid

#67646

Purpose

antisense RNA probe for RNase protection or nothern

Depositor

Stefan Stamm

Article

Falaleeva et al Gene. 2015 Jul 26. pii: S0378-1119(15)00

Insert

MBII85 snoRNA

Use

For in vitro transcription

Available Since

Aug. 4, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
LZRS-GADD45A

Plasmid

#24954

Depositor

Paul Khavari

Article

Sen et al Nature. 2010 Jan 28. 463(7280):563-7.

Insert

GADD45A (GADD45A Human)

Use

Retroviral

Available Since

June 10, 2010

Availability

Academic Institutions and Nonprofits only
pCTCON2-GYB

Plasmid

#160927

Purpose

Dual fluorescent protein reporter (GFP-BFP) for ncAA incorporation (wild-type construct)

Depositor

James Van Deventer

Article

Potts et al Mol Syst Des Eng. 2020 Feb 1;5(2):573-58

Inserts

BFP
sfGFP

Expression

Yeast

Promoter

GAL1-10

Available Since

Jan. 12, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge

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We narrowed to 159,093 results for: Des