Skip to main content
Addgene
Showing: 4041 - 4080 of 17172 results

  24. pSin4 UAP1 F383G

    Plasmid
    #169891
    Purpose
    Used for generation of stable mammalian cell lines expressing UAP1 with with F383G mutation
    Depositor
    Jennifer Kohler
    Article
    Capota et al Curr Protoc. 2021 Jul;1(7):e201. doi: 10
    Insert
    UDP-N-acetylhexosamine pyrophosphorylase (UAP1 Human)
    Use
    Lentiviral
    Tags
    None
    Expression
    Mammalian
    Mutation
    Changed Phenylalanine (F) at position 383 to glyc…
    Promoter
    Available since
    June 17, 2021
    Availability
    Academic Institutions and Nonprofits only
    169891-plasmid-map-sequence-id-333152

    Enlarge

  29. GG-EBNA-OMKSL-PP

    Plasmid
    #102899
    Purpose
    EBNA episome plasmid for U6 promoter-driven expression of 10 gRNAs targeting OCT4, MYC, KLF4, SOX2 and LIN28A promoters. Includes PGK-puro selection cassette.
    Depositor
    Timo Otonkoski
    Article
    Weltner et al Nat Commun. 2018 Jul 6;9(1):2643. doi: 1
    Insert
    OMKSL-gRNAS-PGK-puro (POU5F1, MYC, KLF4, SOX2, LIN28A Human)
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6
    Available since
    July 10, 2018
    Availability
    Academic Institutions and Nonprofits only
    102899-plasmid-map-sequence-id-198366

    Enlarge

  31. LentiGuide Cherry

    Plasmid
    #170510
    Purpose
    Expresses S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from U6 promoter and mCherry marker from EF-1a promoter. 3rd generation lentiviral backbone.
    Depositor
    Pavel Tolar
    Article
    Newman et al biorXiv 2021.03.02.433398
    Inserts
    S. pyogenes sgRNA cassette
    mCherry
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF-1a and hU6
    Available since
    Sept. 27, 2021
    Availability
    Academic Institutions and Nonprofits only
    170510-plasmid-map-sequence-id-343694

    Enlarge

  37. pORF1-Flag

    Plasmid
    #190565
    Purpose
    Mammalian expression plasmid encoding the first open reading frame (ORF1, expressing the protein ORF1p) of human L1 (L1.3); carries a C-terminus Flag tag.
    Depositor
    Anthony Furano
    Article
    Cook et al Proc Natl Acad Sci U S A. 2015 Apr 7;112
    Insert
    ORF1 (from L1.3)
    Use
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    May 16, 2023
    Availability
    Academic Institutions and Nonprofits only
    190565-plasmid-map-sequence-id-393221

    Enlarge

  39. pICE-HA-NLS-I-PpoI

    Plasmid
    #46963
    Purpose
    For constitutive or doxycycline-inducible expression of the site specific homing nuclease I-PpoI (human codon optimized). Confers resistance to puro. Use T-REx cells for doxy-inducible expression.
    Depositor
    Steve Jackson
    Article
    Britton et al J Cell Biol. 2013 Jul 29.
    Insert
    NLS-I-PpoI
    Use
    Tags
    HA
    Expression
    Mammalian
    Mutation
    Promoter
    CMV-TET
    Available since
    Aug. 20, 2013
    Availability
    Academic Institutions and Nonprofits only
    46963-plasmid-map-sequence-id-66854

    Enlarge

Showing: 4041 - 4080 of 17172 results