-
Plasmid#213051PurposeModular Unit carrying pegRNA and ngRNA scaffolds flanked by attL1 and attR3DepositorInsertsgRNA
UseTagsExpressionBacterialMutationPromoter35S-CmYCLV-AtU6Available sinceFeb. 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFBOT563
Plasmid#40864DepositorInsertOxytocin promoter
UseAAVTagsExpressionBacterial and InsectMutationPromotermouse oxytocin promoterAvailable sinceNov. 2, 2012AvailabilityAcademic Institutions and Nonprofits only -
pBBR1k-J23101-GFPuv
Plasmid#106388PurposepBBR1k-GFPuv with Anderson promoter J23101 expressing lacIDepositorInsertGFPuv
UseTagsExpressionBacterialMutationPromotertrcAvailable sinceMarch 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
ATF4 5: 5’ATF4.GFP
Plasmid#21852DepositorInsertATF4
UseTagsEGFPExpressionMammalianMutationPromoterAvailable sinceSept. 10, 2009AvailabilityAcademic Institutions and Nonprofits only -
pCfB2191
Plasmid#67538PurposeEasyClone system-based yeast integrative vector carrying loxP-flanked KlURA3 marker, integration into S. cerevisiae XII-1 chromosomal location, USER site for cloning, amp resistanceDepositorTypeEmpty backboneUseCre/Lox; IntegrativeTagsExpressionYeastMutationPromoterAvailable sinceMarch 10, 2016AvailabilityAcademic Institutions and Nonprofits only -
pL1P4 ZmUbiP:GRF-GIF:NosT
Plasmid#198048PurposeMoClo Golden Gate Level 1 Position 4. Overexpression cassette of Growth-Regulating Factor 4 (GRF4) plus GRF-Interacting Factor 1 (GRF4-GIF1). Improves in vitro wheat regeneration and transformation.InsertZmUbiP::TaGRF4-GIF1::NosT
UseSynthetic BiologyTagsExpressionMutationPromoterZea mays (maize) ubiquitin promoterAvailable sinceApril 19, 2023AvailabilityAcademic Institutions and Nonprofits only -
pH6HTC_FAM98A-Halo
Plasmid#175329PurposeProduction of HaloTag fusion proteinDepositorInsertFAM98A (FAM98A Human)
UseCell free expressionTagsHaloTag-6xHisExpressionBacterialMutationPromoterAvailable sinceOct. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3 hE47
Plasmid#16059DepositorInsertE47 (TCF3 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 2, 2007AvailabilityAcademic Institutions and Nonprofits only -
pNBU2_erm-TetR-P1T_DP-GH023
Plasmid#90324PurposeIntegration vector at an attB site in Bacteroides; contains 1kb cassette with both the P1T_DP aTC-inducible promoter (RBS GH023) and the consitutively-driven TetR by the P2-A21 promoterDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceMay 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
pG12-SEAP
Plasmid#170271PurposeSecreted alkaline phosphatase (SEAP) expression in mammalian cells. Reporter plasmid.DepositorInsertSEAP
UseTagsExpressionMammalianMutationPromoter12xUAS with TATA-box minimal promoterAvailable sinceJune 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
p005-RFP-strong
Plasmid#108317PurposeSynthetic RFP based on Corynactis spp under strong synthetic constitutive promoter pFAB4005; kanamycin resistant.DepositorInsertRFP under controlf of strong synthetic constitutive promoter pFAB4005
UseTagsExpressionBacterialMutationPromoterpFAB4005Available sinceAug. 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
PKC alpha DN
Plasmid#21235DepositorInsertPKC alpha (PRKCA Human)
UseTagsHAExpressionMammalianMutationK368RPromoterAvailable sinceAug. 13, 2009AvailabilityAcademic Institutions and Nonprofits only -
pγsat
Plasmid#39238DepositorInsert8 copies of the 234bp mouse γ-satellite repeat
UseCloning vectorTagsExpressionMutationPromoterAvailable sinceJan. 16, 2013AvailabilityAcademic Institutions and Nonprofits only -
MCP-EGFP-PiggyBac
Plasmid#190003PurposeExpression of MCP-EGFP in mammalian cells. To clone this plasmid, Addgene plasmid #119908 was used. We replaced the tandem coat proteins and double tagRFP with a single coat protein fused to EGFP.DepositorInsertMCP-EGFP
UseTagsExpressionMutationPromoterAvailable sinceNov. 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET15b-KSRP
Plasmid#23019DepositorInsertKSRP (KHSRP Human)
UseTagsHisExpressionBacterialMutationPromoterAvailable sinceJune 4, 2010AvailabilityAcademic Institutions and Nonprofits only -
-
pSADdeltaG-F3
Plasmid#32634DepositorInsertB19N, B19P, B19M and B19L
UseTagsExpressionMammalianMutationIn frame insertion of Ser after aa10 in M (matrix…PromoterCMVAvailable sinceOct. 26, 2011AvailabilityAcademic Institutions and Nonprofits only -
pSALECT-EcoBam
Plasmid#59705PurposeFolding reporter with ampicillin resistance as a readoutDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 1, 2014AvailabilityAcademic Institutions and Nonprofits only -
pRibo EcoHind
Plasmid#36252DepositorTypeEmpty backboneUseSynthetic Biology; Mycobacteria replicating vectorTagsExpressionBacterialMutationPromoterModified Hsp60Available sinceApril 9, 2012AvailabilityAcademic Institutions and Nonprofits only -
pCfB2223
Plasmid#67544PurposeEasyClone system-based yeast integrative vector carrying loxP-flanked kanMX marker, integration into S. cerevisiae X-3 chromosomal location, USER site for cloning, amp resistanceDepositorTypeEmpty backboneUseCre/Lox; IntegrativeTagsExpressionYeastMutationPromoterAvailable sinceMarch 10, 2016AvailabilityAcademic Institutions and Nonprofits only -
PKC alpha CAT
Plasmid#21234DepositorInsertPKC alpha (PRKCA Human)
UseTagsHAExpressionMammalianMutationdelta 1-325PromoterAvailable sinceAug. 13, 2009AvailabilityAcademic Institutions and Nonprofits only -
pEXP5-NT/pT7 LasI
Plasmid#193631PurposeConstitutive T7 RNAP-mediated expression of LasI 3OC12-HSL synthase for Las quorum sensing system. LasI sequence is codon optimized for E. coli.DepositorInsertLasI
UseTags6xHisExpressionBacterialMutationPromoterT7 RNAP promoterAvailable sinceJuly 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBbB6c-GFP
Plasmid#35356DepositorInsertGFP
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterLlacO-1Available sinceFeb. 20, 2012AvailabilityAcademic Institutions and Nonprofits only -
pEXP5-NT/pT7 LuxI
Plasmid#193626PurposeConstitutive T7 RNAP-mediated expression of LuxI 3OC6-HSL synthase for Lux quorum sensing system. LuxI sequence is codon optimized for E. coli.DepositorInsertLuxI
UseTags6xHisExpressionBacterialMutationPromoterT7 RNAP promoterAvailable sinceJuly 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBBR2k-GFPuv
Plasmid#106383PurposeAnhydrotetracycline-inducible tet promoter expressing gfpuv on BBR1 originDepositorInsertGFPuv
UseTagsExpressionBacterialMutationPromotertetAvailable sinceMarch 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBbB8c-GFP
Plasmid#35362DepositorInsertGFP
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterBADAvailable sinceMarch 2, 2012AvailabilityAcademic Institutions and Nonprofits only -
mCD8-GFP in pUAST
Plasmid#17746DepositorInsertmouse CD8 (Cd8a Mouse)
UseTagsGFPExpressionInsectMutationPromoterAvailable sinceMay 14, 2008AvailabilityAcademic Institutions and Nonprofits only -
-
pBbB0c-RFP
Plasmid#35366DepositorInsertRFP
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceMarch 1, 2012AvailabilityAcademic Institutions and Nonprofits only -
-
LbCas12a-gRNA_BbsI_CAM
Plasmid#183222PurposeLbCas12a gRNA containing BbsI restriction recognition sites in spacer sequence for Golden Gate AssemblyDepositorInsertLbCas12a guide RNA containing BbsI sites in spacer sequence
UseCRISPRTagsExpressionBacterialMutationPromoterAvailable sinceJune 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCHW00019
Plasmid#222448PurposeLevel 0, C terminal tag module (TTCG-GCTT) containing GAL4AD-NLS.DepositorInsertGAL4AD-NLS
UseSynthetic BiologyTagsExpressionPlantMutationnoPromoterAvailable sinceDec. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCHW100003
Plasmid#222442PurposeLevel 0, N-terminal tag module (CCAT-AATG) containing mCherry2-HyP5SM.DepositorInsertmCherry2-HyP5SM
UseSynthetic BiologyTagsExpressionPlantMutationnoPromoterAvailable sinceNov. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCHW111114
Plasmid#222459PurposeLevel 1 acceptor, Position 3 containing CBS4-CMV1::LacZ::nosT.DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionPlantMutationnoPromoterCBS4-CMV1 (Cucumber mosaic virus)Available sinceNov. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCHW111118
Plasmid#222461PurposeLevel 1 acceptor, Position 3 containing CBS4-CMV1::LoxP-mCherry-stop-LacZ::nosT.DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionPlantMutationnoPromoterCBS4-CMV1 (Cucumber Mosaic Virus)Available sinceNov. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCHW111113
Plasmid#222458PurposeLevel 1 acceptor, Position 3 containing CBS4-CMV1::LacZ::nosT.DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionPlantMutationnoPromoterCBS4-CMV1 (Cucumber mosaic virus)Available sinceNov. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCHW91013
Plasmid#222450PurposeLevel 1, Position 3 containing CBS4-miniDFR::RUBY::OCS.DepositorInsertCBS4-miniDFR::RUBY::OCS
UseSynthetic BiologyTagsExpressionPlantMutationnoPromoterAvailable sinceNov. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCHW91003
Plasmid#222449PurposeLevel 1, Position 3 containing 35S::RUBY::OCS.DepositorInsert35S::RUBY::OCS
UseSynthetic BiologyTagsExpressionPlantMutationnoPromoterAvailable sinceNov. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCHW91070
Plasmid#222463PurposeLevel 1 acceptor, Position 3 containing CBS4-CMV1::mCherry2-HyP5SM-LacZ::nosT.DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionPlantMutationnoPromoterCBS4-CMV1 (Cucumber Mosaic Virus)Available sinceNov. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pVT36b
Plasmid#184437PurposeCRISPR/Cas9 plasmid, containing ScARS, IoURA3, iCas9, RPR1’-tRNA_Leu promoter, and sgRNA scaffoldDepositorTypeEmpty backboneUseCRISPRTagsExpressionYeastMutationPromoterAvailable sinceMay 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLTR-RVG
Plasmid#17577DepositorInsertrabies virus glycoprotein (SAD B19 strain)
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceApril 14, 2008AvailabilityAcademic Institutions and Nonprofits only -
pEXP5-NT/pT7 LuxR
Plasmid#193625PurposeConstitutive T7 RNAP-mediated expression of LuxR transcription factor for Lux quorum sensing system. LuxR sequence is codon optimized for E. coli.DepositorInsertLuxR
UseTags6xHisExpressionBacterialMutationPromoterT7 RNAP promoterAvailable sinceJuly 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLVET-tTR-KRAB
Plasmid#11644PurposeTet-regulated (Tet-on) lentiviral vector for transgene (hEF-1alpha promoter) - OR - shRNA (H1 promoter when subcloned from pLVTHM (Addgene#12247)) - 2nd generationDepositorInserthEF-1alpha, GFP, tTR-KRAB, Tet-on
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceAug. 17, 2006AvailabilityAcademic Institutions and Nonprofits only -
pEXP5-NT/pT7 LasR
Plasmid#193629PurposeConstitutive T7 RNAP-mediated expression of LasR transcription factor for Las quorum sensing system. LasR sequence is codon optimized for E. coli.DepositorInsertLasR
UseTags6xHisExpressionBacterialMutationPromoterT7 RNAP promoterAvailable sinceJuly 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLVPT-tTR-KRAB
Plasmid#11642PurposeTet-regulated (Tet-on) lentiviral vector for transgene (hPGK promoter) - AND/OR - shRNA (H1 promoter when subcloned from pLVTHM (Addgene#12247)) - 2nd generationDepositorInsertmPGK, GFP, tTR-KRAB, Tet-on
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceAug. 17, 2006AvailabilityAcademic Institutions and Nonprofits only -
pNC-Green-LUC
Plasmid#193438PurposeNimble cloning-compatible vector for the analysis of promoter activity in plantsDepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterAvailable sinceDec. 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
Cherry-LacRep
Plasmid#18985DepositorInsertLacI
UseTagsAviTag, SV40 NLS, and mCherryExpressionMammalianMutationPromoterCMVAvailable sinceAug. 20, 2008AvailabilityAcademic Institutions and Nonprofits only -
pAR-Ec318
Plasmid#130871PurposeA nuclear plasmid encoding the WT TP-DNAP1 for OrthoRepDepositorInsertTP-DNAP1
UseTagsExpressionYeastMutationPromoterAvailable sinceAug. 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
p1324 HPV-16 E7
Plasmid#8643DepositorInsertHPV-16 E7 (E7 Human papillomavirus)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 16, 2005AvailabilityAcademic Institutions and Nonprofits only -
M-NM-sgRNA
Plasmid#48673PurposeMammalian U6-driven sgRNA (NMm1) targeting GTCCCCTCCACCCCACAGTGDepositorInsertsgRNA targeting GTCCCCTCCACCCCACAGTG, compatible with N. meningitidis Cas9, hU6 promoter
UseCRISPRTagsExpressionMutationPromoterhU6Available sinceOct. 22, 2013AvailabilityAcademic Institutions and Nonprofits only