We narrowed to 4,492 results for: BRE

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pLenti-EF1a-Ngn3ER-PuroR-mCherry

Plasmid

#197385

Purpose

To establish Ngn3ER cell line for endocrine differentiation (4-hydroxytamoxifen activation, Puromycin resistant)

Depositor

Qiao Zhou

Article

Huang et al Nat Cell Biol. 2023 Apr 27. doi: 10.1038

Insert

Ngn3ER-2A-PuroR-2A-mCherry

Use

Lentiviral

Expression

Mammalian

Promoter

EF1A

Available Since

March 8, 2023

Availability

Academic Institutions and Nonprofits only

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pLenti-TetO-PM

Plasmid

#197387

Purpose

To establish all-in-one TetO-inducible cell line for Pdx1-Mafa expression (Doxycycline induced; rtTA included; Blasticidin resistant)

Depositor

Qiao Zhou

Article

Huang et al Nat Cell Biol. 2023 Apr 27. doi: 10.1038

Insert

PDX1-T2A-MAFA

Use

Lentiviral; Teto

Expression

Mammalian

Promoter

TRE

Available Since

Feb. 23, 2023

Availability

Academic Institutions and Nonprofits only

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BPK1520_puroR

Plasmid

#173901

Purpose

SpCas9 guide cloning vector derived from BPK1520 that allows for puromycin-based selection

Depositor

Ronald Cohn

Article

Erwood et al Genome Res. 2019 Dec;29(12):2010-2019. d

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Promoter

CMV/U6

Available Since

Nov. 1, 2021

Availability

Academic Institutions and Nonprofits only

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pU6-sp-sgRNA-mDNMT1_nick-lenti

Plasmid

#135959

Purpose

S. pyogenes nicking sgRNA for mouse DNMT1

Depositor

David Liu

Article

Anzalone et al Nature. 2019 Oct 21. pii: 10.1038/s41586

Insert

mDNMT1_nick

Expression

Mammalian

Mutation

See manuscript

Promoter

U6

Available Since

Jan. 2, 2020

Availability

Academic Institutions and Nonprofits only

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raACR-TS-Kv-P2A-mCerulean3-pAAV-CaMKII

Plasmid

#204971

Purpose

Soma-targeted neuronal expression of the MsACR1 mutant raACR and mCerulean3 separated by a P2A site.

Depositor

Peter Hegemann

Article

Oppermann et al eLife. 2024 Oct 14;12:RP90100. doi: 10.7

Insert

raACR

Use

AAV

Tags

mCerulean3

Promoter

CaMKII

Available Since

Oct. 25, 2023

Availability

Academic Institutions and Nonprofits only

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pENTRY-GFP

Plasmid

#15301

Depositor

William Hahn

Article

Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

Insert

hrGFP

Expression

Mammalian

Available Since

July 12, 2007

Availability

Academic Institutions and Nonprofits only
pN1-CMV-sDarken

Plasmid

#184799

Purpose

Serotonin Sensor sDarken under the control of a CMV Promoter.

Depositor

Olivia Masseck

Article

Kubitschke et al Nat Commun. 2022 Dec 6;13(1):7525. doi:

Insert

sDarken

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 2, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-fPV-GFP

Plasmid

#22914

Depositor

Edward Callaway

Article

Nathanson et al Front Neural Circuits. 2009 Nov 9;3:19.

Insert

fugu parvalbumin promoter driving GFP

Use

AAV

Expression

Mammalian

Available Since

May 25, 2010

Availability

Academic Institutions and Nonprofits only

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pAAV-NanoLuc-15aa linker-βarrestin2-no HA-GS linker-TEVp

Plasmid

#125235

Purpose

Expresses SPARK2 NanoLuc-βarrestin2-TEVp (no HA) in mammalian cells

Depositor

Alice Ting

Article

Kim et al eLife. 2019 Apr 3;8. pii: 43826. doi: 10

Insert

NanoLuc-15aa linker-βarrestin2-no HA-GS linker-TEVp

Use

AAV

Expression

Mammalian

Promoter

CMV

Available Since

May 9, 2019

Availability

Academic Institutions and Nonprofits only

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pSL0341 (pTarget)

Plasmid

#200906

Purpose

Encodes an mCherry casette under a minimal CMV promoter. Used as the target plasmid for plasmid-based integration assays, and for plasmid-based transcriptional activation.

Depositor

Samuel H. Sternberg

Article

Lampe et al Nat Biotechnol. 2023 Mar 29. doi: 10.103

Insert

pTarget

Expression

Mammalian

Available Since

May 23, 2023

Availability

Academic Institutions and Nonprofits only

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pCDNA5/FRT_CMV_NES-Kinprola_JNK-mEGFP

Plasmid

#233359

Purpose

CMV driven expression of the JNK activity recorder Kinprola_JNK in mammalian cell lines fused to mEGFP

Depositor

Kai Johnsson

Article

Sun et al Nat Chem Biol. 2025 Jul 10. doi: 10.1038

Insert

NES-Kinprola_JNK-mEGFP

Tags

NES and mEGFP

Expression

Mammalian

Promoter

CMV

Available Since

March 26, 2025

Availability

Academic Institutions and Nonprofits only

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pCDNA5/FRT_CMV_NES-Kinprola_AMPK-mEGFP

Plasmid

#233361

Purpose

CMV driven expression of the AMPK activity recorder Kinprola_AMPK in mammalian cell lines fused to mEGFP

Depositor

Kai Johnsson

Article

Sun et al Nat Chem Biol. 2025 Jul 10. doi: 10.1038

Insert

NES-Kinprola_AMPK-mEGFP

Tags

NES and mEGFP

Expression

Mammalian

Promoter

CMV

Available Since

March 26, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-CMV-SpyCas9

Plasmid

#113034

Purpose

AAV vector for expression of SpyCas9 in mammalian cells

Depositor

Dominik Niopek

Article

Bubeck et al Nat Methods. 2018 Oct 30. pii: 10.1038/s

Insert

SpyCas9

Use

AAV, CRISPR, and Synthetic Biology

Expression

Mammalian

Available Since

Oct. 30, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA5-FRT/TO-Flag-RNF169

Plasmid

#74243

Purpose

Expresses RNF169 in mammalian cells

Depositor

Daniel Durocher

Article

Panier et al Mol Cell. 2012 Aug 10;47(3):383-95. doi:

Insert

RNF169 (RNF169 Human)

Tags

Flag

Expression

Mammalian

Promoter

CMV

Available Since

April 20, 2016

Availability

Industry, Academic Institutions, and Nonprofits

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pspCas9-Klenow-ST2-com-vector

Plasmid

#176235

Purpose

Vector plasmid for expressing spCas9-Klenow fusion protein and sgRNA. There are two copies of HBB 3’ UTR in the 3’UTR of spCas9-Klenow to enhance expression.

Depositor

Baisong Lu

Article

Yoo et al Nucleic Acids Res. 2022 Mar 22. pii: 655

Type

Empty backbone

Expression

Bacterial

Available Since

March 30, 2022

Availability

Academic Institutions and Nonprofits only

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pmiRFP703-Tubulin

Plasmid

#79991

Purpose

Alpha tubulin labeling with near-infrared fluorescent protein miRFP703

Depositor

Vladislav Verkhusha

Article

Shcherbakova et al Nat Commun. 2016 Aug 19;7:12405. doi: 10

Insert

Tubulin (TUBA1B Human)

Tags

miRFP703

Expression

Mammalian

Promoter

CMV

Available Since

July 20, 2016

Availability

Academic Institutions and Nonprofits only

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pBABE-hygro LAP2

Plasmid

#15740

Depositor

Joan Massague

Article

Gomis et al Cancer Cell. 2006 Sep . 10(3):203-14.

Insert

LAP2 (CEBPB Human)

Use

Retroviral

Tags

HA

Expression

Mammalian

Available Since

Sept. 7, 2007

Availability

Academic Institutions and Nonprofits only
pEF.DEST51-VPS35-mScarlet

Plasmid

#215474

Purpose

Expression vector with VPS35 tagged with mScarlet

Depositor

Johanna Ivaska

Article

Conway et al Nat Cell Biol. 2025 May 26. doi: 10.1038

Insert

VPS35 (VPS35 Human)

Tags

mScarlet

Expression

Mammalian

Promoter

EF1a

Available Since

May 29, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pBABE-puro LAP2

Plasmid

#15712

Depositor

Joan Massague

Article

Gomis et al Cancer Cell. 2006 Sep . 10(3):203-14.

Insert

LAP2 (CEBPB Human)

Use

Retroviral

Expression

Mammalian

Available Since

Sept. 7, 2007

Availability

Academic Institutions and Nonprofits only
pcDNA-3xflagGREB1a

Plasmid

#112221

Purpose

mammalian expression of flag tagged GREB1a

Depositor

Craig Burd

Article

Haines et al Endocr Relat Cancer. 2018 Jul;25(7):735-

Insert

GREB1a (GREB1 Human)

Tags

3x flag

Expression

Mammalian

Available Since

July 20, 2018

Availability

Academic Institutions and Nonprofits only

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