We narrowed to 157,143 results for: Des

1. pDEST27-GST-ATXN1 FL(84Q)-S776A

   Plasmid

   #21758

   Depositor

   Huda Zoghbi

   Article

   Lim et al Nature. 2008 Apr 10. 452(7188):713-8.

   Insert

   Ataxin-1 (ATXN1 Human)
   

   Tags

   GST

   Expression

   Mammalian

   Mutation

   insert contains a stretch of 84 Q(Glutamines) and…

   Promoter

   CMV

   Available Since

   July 22, 2014

   Availability

   Academic Institutions and Nonprofits only

2. pDEST27-GST-ATXN1 FL(30Q)-S776A

   Plasmid

   #21756

   Depositor

   Huda Zoghbi

   Article

   Lim et al Nature. 2008 Apr 10. 452(7188):713-8.

   Insert

   Ataxin-1 (ATXN1 Human)
   

   Tags

   GST

   Expression

   Mammalian

   Mutation

   insert contains a stretch of 32 amino acids (30 Q…

   Promoter

   CMV

   Available Since

   July 22, 2014

   Availability

   Academic Institutions and Nonprofits only

3. pEF5/FRT-DEST EVC2ala4-Flag

   Plasmid

   #41008

   Depositor

   Rajat Rohatgi

   Article

   Dorn et al Dev Cell. 2012 Sep 12. pii: S1534-5807(1

   Insert

   Ellis van Creveld syndrome 2 (EVC2 Mouse)
   

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   changed amino acids 1183-1186 to Alanine

   Available Since

   Nov. 15, 2012

   Availability

   Academic Institutions and Nonprofits only

4. pMef2c-AHF-DEST (JDW 476)

   Plasmid

   #242577

   Purpose

   Gateway destination vector with the murine Mef2c anterior heart field promoter in the backbone.

   Depositor

   Joshua Wythe

   Article

   Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

   Type

   Empty backbone

   Expression

   Mammalian

   Available Since

   Sept. 24, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pEF.DEST51-PTPN11(D425A,C459S)-Clover

   Plasmid

   #215526

   Purpose

   Expression vector with a clover-tagged phosphatase-inactive Shp2 mutant (D425A,C459S)

   Depositor

   Johanna Ivaska

   Article

   Conway et al Nat Cell Biol. 2025 May 26. doi: 10.1038

   Insert

   PTPN11 (PTPN11 Human)
   

   Tags

   Clover

   Expression

   Mammalian

   Mutation

   D425A, C459S (Phosphatase inactive)

   Promoter

   EF1a

   Available Since

   Sept. 3, 2025

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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6. pDEST-hSTARR-luc-Pmyc-ccw

   Plasmid

   #158027

   Purpose

   This is a dual function destination vector for eSTARR-seq and luciferase assay to quantify enhancer activity. It contains a MYC promoter, a luciferase CDS, followed by a Gateway cassette (R2-R1).

   Depositor

   Haiyuan Yu

   Article

   Tippens et al Nat Genet. 2020 Oct;52(10):1067-1075. do

   Type

   Empty backbone

   Use

   Luciferase

   Expression

   Mammalian

   Promoter

   MYC

   Available Since

   Aug. 6, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. pMC-dest-BsaI-cmlc2:mCherry

   Plasmid

   #241213

   Depositor

   Junsu Kang

   Type

   Empty backbone

   Use

   Minicircle cloning vector

   Mutation

   TCGC linker

   Available Since

   Aug. 5, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. pEF.DEST51-PTPN12(D199A,C231S)-Clover

   Plasmid

   #215528

   Purpose

   Expression vector with a clover-tagged phosphatase-inactive PTP-PEST mutant (D199A,C231S)

   Depositor

   Johanna Ivaska

   Article

   Conway et al Nat Cell Biol. 2025 May 26. doi: 10.1038

   Insert

   PTPN12 (PTPN12 Human)
   

   Tags

   Clover

   Expression

   Mammalian

   Mutation

   D199A, C231S (Phosphatase inactive)

   Promoter

   EF1a

   Available Since

   May 29, 2025

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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9. pLPC-C-SF-TAP-DEST-TLK1- M566T

   Plasmid

   #218831

   Purpose

   Expresses C-terminal Strep-FLAG-tagged M566T human TLK1 variant 5 in a retrovirus vector.

   Depositor

   Travis Stracker

   Insert

   TLK1 M566T (TLK1 Human)
   

   Tags

   FLAG-STREP

   Expression

   Mammalian

   Mutation

   M566T

   Promoter

   CMV

   Available Since

   July 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLPC-C-SF-TAP-DEST-TLK1- Q479E

    Plasmid

    #218830

    Purpose

    Expresses C-terminal Strep-FLAG-tagged Q479E human TLK1 variant 5 in a retrovirus vector.

    Depositor

    Travis Stracker

    Insert

    TLK1 Q479E (TLK1 Human)
    

    Tags

    FLAG-STREP

    Expression

    Mammalian

    Mutation

    Q479E

    Promoter

    CMV

    Available Since

    July 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pDestTol2-QUAS:GFP-CAAX-he1.1:YFP

    Plasmid

    #184816

    Purpose

    Used to generate the Tg(QUAS:GFP-CAAX; he1.1:YFP)c631 transgenic line

    Depositor

    Marnie Halpern

    Article

    Spikol et al Elife. 2024 May 31;12:RP89516. doi: 10.7

    Insert

    QUAS:GFP-CAAX-pA; he1.1:CFP
    

    Use

    Zebrafish tol2 transgenesis

    Tags

    GFP-CAAX

    Available Since

    May 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pDestTol2-QUAS:NLS-mApple-he1.1:CFP

    Plasmid

    #184814

    Purpose

    Used to generate the Tg(QUAS:NLS-mApple; he1.1:CFP)c718 transgenic line

    Depositor

    Marnie Halpern

    Article

    Spikol et al Elife. 2024 May 31;12:RP89516. doi: 10.7

    Insert

    QUAS:NLS-mApple-pA; he1.1:CFP
    

    Use

    Zebrafish tol2 transgenesis

    Tags

    NLS-mApple

    Available Since

    May 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pDestTol2-QUAS:NLS-GFP-he1.1:CFP

    Plasmid

    #184815

    Purpose

    Used to generate the Tg(QUAS:NLS-GFP; he1.1:CFP)c682 transgenic line

    Depositor

    Marnie Halpern

    Article

    Spikol et al Elife. 2024 May 31;12:RP89516. doi: 10.7

    Insert

    QUAS:NLS-GFP-pA; he1.1:CFP
    

    Use

    Zebrafish tol2 transgenesis

    Tags

    NLS-GFP

    Available Since

    May 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pDEST17-His10-Smt3-ccdB-SNAP

    Plasmid

    #166148

    Purpose

    Gateway destination vector for generation of N-terminal His10-Smt3 and C-terminal SNAP tagged proteins in E. coli.

    Depositor

    Daniel Jarosz

    Article

    Harel et al Cell Rep. 2024 Jun 25;43(6):112787. doi:

    Type

    Empty backbone

    Tags

    His10-Smt3 and SNAP

    Expression

    Bacterial

    Available Since

    May 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pDEST17-His10-Smt3-DDX5(kf)-SNAP

    Plasmid

    #166149

    Purpose

    Expression of N. furzeri DDX5 in E. coli

    Depositor

    Daniel Jarosz

    Article

    Harel et al Cell Rep. 2024 Jun 25;43(6):112787. doi:

    Insert

    DDX5
    

    Tags

    His10-Smt3 and SNAP

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    May 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pDEST17-His10-Smt3-DDX5ΔPrD(kf)-SNAP

    Plasmid

    #166150

    Purpose

    Expression of N. furzeri DDX5ΔPrD(1-535) mutant in E. coli

    Depositor

    Daniel Jarosz

    Article

    Harel et al Cell Rep. 2024 Jun 25;43(6):112787. doi:

    Insert

    DDX5ΔPrD(1-535)
    

    Tags

    His10-Smt3 and SNAP

    Expression

    Bacterial

    Mutation

    ΔPrD(1-535)

    Promoter

    T7

    Available Since

    May 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pDEST17-His10-Smt3-DDX5ΔIDR(kf)-SNAP

    Plasmid

    #166151

    Purpose

    Expression of N. furzeri DDX5ΔIDR(1-483) mutant in E. coli

    Depositor

    Daniel Jarosz

    Article

    Harel et al Cell Rep. 2024 Jun 25;43(6):112787. doi:

    Insert

    DDX5ΔIDR(1-483)
    

    Tags

    His10-Smt3 and SNAP

    Expression

    Bacterial

    Mutation

    ΔIDR(1-483)

    Promoter

    T7

    Available Since

    May 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pDN0617 (pDEST-mEGFP C-term, NatR)

    Plasmid

    #210484

    Purpose

    pDEST vector to tag gene of interest with mEGFP on the C-terminus to perform localization assays in S. cerevisiae.

    Depositor

    Christian Landry

    Article

    Evans-Yamamoto et al Mol Biol Evol. 2023 Nov 18:msad246. doi:

    Type

    Empty backbone

    Use

    Synthetic Biology; Destination vector for gateway…

    Tags

    mEGFP

    Expression

    Yeast

    Promoter

    ADH1

    Available Since

    Dec. 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pDEST-Spe4-Kozak-emiRFP703-RfA

    Plasmid

    #186376

    Purpose

    Destination vector for the expression of a fluorescent fusion of N-ter emiRFP703 and a protein of interest into embryos, by mRNA microinjection

    Depositor

    Patrick Lemaire

    Type

    Empty backbone

    Use

    Gateway destination vector

    Available Since

    June 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pDEST-Myc-FBW7 Delta WD40 (-2 to 7)

    Plasmid

    #197454

    Purpose

    The plasmid expresses delta WD40 deleted version of Myc-tagged FBW7 human isoform 1. WD40 propellars 2 to 7 are deleted. Used for mammalian overexpression and detection by western blotting.

    Depositor

    Omar Khan

    Article

    Khan et al Nat Commun. 2021 Apr 6;12(1):2043. doi:

    Insert

    FBW7 Delta-WD40 propeller (FBXW7 Human)
    

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    FBW& dimerization domain deleted.

    Promoter

    CMV

    Available Since

    June 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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