We narrowed to 7,608 results for: Pol

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pEx-deletion-erm

Plasmid

#172213

Purpose

For allelic exchange in erythromycin-sensitive Prevotella copri strains

Depositor

Till Strowig

Article

Li et al EMBO J. 2021 Dec 1;40(23):e108287. doi:

Type

Empty backbone

Use

Allelic exchange in prevotella copri

Available Since

May 18, 2022

Availability

Academic Institutions and Nonprofits only

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pTP284

Plasmid

#113312

Purpose

human Nbs1 in baculovirus transfer vector

Depositor

Tanya Paull

Article

Myler et al Mol Cell. 2017 Sep 7;67(5):891-898.e4. d

Insert

Nbs1 (NBN Human)

Expression

Insect

Promoter

polyhedrin

Available Since

Sept. 18, 2018

Availability

Academic Institutions and Nonprofits only

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pDONR223-PLK2

Plasmid

#23691

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

PLK2 (PLK2 Human)

Use

Gateway Cloning

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only

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pEF-BOS-T7-CA150

Plasmid

#21924

Depositor

Mariano Garcia-Blanco

Article

Sune et al Mol Cell Biol. 1999 Jul . 19(7):4719-28.

Insert

CA150 (TCERG1 Human)

Tags

T7

Expression

Mammalian

Available Since

Sept. 4, 2009

Availability

Academic Institutions and Nonprofits only
pCLX-M38s/TOP-dGFP

Plasmid

#114295

Purpose

Expression of destabilized GFP under the control of the M38-TOP canonical Wnt signaling responsive promoter

Depositor

Patrick Salmon

Article

Boitard et al Cell Rep. 2015 Mar 3;10(8):1349-61. doi:

Insert

dGFP

Use

Lentiviral

Mutation

destabilized GFP

Available Since

Sept. 11, 2018

Availability

Academic Institutions and Nonprofits only

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pYIAL31

Plasmid

#60807

Purpose

used to make mutations in yeast POL1

Depositor

Thomas Kunkel, Youri Pavlov

Article

Pavlov et al Genetics. 2001 Sep;159(1):47-64.

Insert

Pol1 (POL1 Budding Yeast)

Expression

Yeast

Mutation

N-terminal part only

Available Since

Feb. 6, 2015

Availability

Academic Institutions and Nonprofits only

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Ube2v1

Plasmid

#31429

Depositor

Rachel Klevit

Article

Christensen et al Nat Struct Mol Biol. 2007 Oct . 14(10):9

Insert

Ubiquitin Conjugating Enzyme (UBE2V1 Human)

Tags

HA

Expression

Yeast

Available Since

Oct. 27, 2011

Availability

Academic Institutions and Nonprofits only
pTEF:ATP

Plasmid

#92179

Purpose

HO-TEF1p-ATeam1.03-KanMX4-HO. Codon optimized for yeast version of the ATeam1.03 ATP FRET sensor (originally from Imamura et al. PNAS, 2009 ), expressed via the TEF1 promoter. HO locus integration.

Depositor

Matthias Heinemann

Article

Papagiannakis et al Mol Cell. 2017 Jan 19;65(2):285-295. doi

Insert

ATeam1.03 codon optimized for yeast (Saccharomyces cerevisiae)

Use

Integration and expression into yeast.

Tags

Yeast codon optimized mseCFP (donor) and cp173-mV…

Expression

Yeast

Promoter

TEF1p

Available Since

June 26, 2017

Availability

Academic Institutions and Nonprofits only

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gRNA-his-HYB

Plasmid

#64333

Purpose

his3 deletion gRNA cassette carried by pRS42H

Depositor

Yong-Su Jin

Article

Zhang et al Appl Environ Microbiol. 2014 Dec;80(24):

Insert

gBlock product of his3 deletion gRNA cassette

Expression

Yeast

Available Since

April 29, 2015

Availability

Academic Institutions and Nonprofits only
gRNA-leu-HYB

Plasmid

#64332

Purpose

leu2 deletion gRNA cassette carried by pRS42H

Depositor

Yong-Su Jin

Article

Zhang et al Appl Environ Microbiol. 2014 Dec;80(24):

Insert

gBlock product of leu2 deletion gRNA cassette

Expression

Yeast

Available Since

April 29, 2015

Availability

Academic Institutions and Nonprofits only
N-term Hec1 Plk1 FRET sensor c-jun substrate

Plasmid

#45204

Depositor

Michael Lampson

Article

Liu et al J Cell Biol. 2012 Aug 20;198(4):491-9. d

Insert

Hec1-targeted Plk1 FRET sensor

Tags

CyPET, YPET

Expression

Mammalian

Available Since

May 31, 2013

Availability

Academic Institutions and Nonprofits only

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ZE26 Stbm-N-6myc-pCS2+

Plasmid

#17068

Depositor

Randall Moon

Article

Park et al Nat Cell Biol. 2002 Jan;4(1):20-5.

Insert

Stbm (vangl2 Zebrafish)

Use

Zebrafish expression

Tags

Myc6

Available Since

Feb. 1, 2008

Availability

Academic Institutions and Nonprofits only
pFastBac K-Flag Ring1A

Plasmid

#1995

Depositor

Robert Kingston

Article

Lavigne et al Mol Cell 2004 Feb 13;13(3):415-25.

Insert

Ring1A (Ring1 Mouse)

Tags

flag

Expression

Insect

Mutation

Kozak before start codon

Available Since

March 23, 2006

Availability

Academic Institutions and Nonprofits only
plk1 T210A

Plasmid

#132965

Purpose

To study the processes involved in early mitotic events, mitotic progression, and control of re-entry after DNA damage

Depositor

Michael Yaffe

Article

Kishi et al Mol Cell Biol. 2009 Jun;29(11):3134-50.

Insert

polo-like kinase 1 T210A (PLK1 Human)

Expression

Mammalian

Mutation

T210A

Available Since

Dec. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pACEBac1-CoRESTfl

Plasmid

#109156

Purpose

Encodes human full-length CoREST with C-terminal 3xFLAG tag to be expressed in a baculovirus/insect cell expression system

Depositor

Monika Golas

Article

Inui et al Protein Sci. 2017 May;26(5):997-1011. do

Insert

human full-length CoREST, sequence-optimized for insect cells (RCOR1 Human)

Tags

3xFLAG

Expression

Insect

Promoter

Polyhedrin

Available Since

Jan. 11, 2019

Availability

Academic Institutions and Nonprofits only

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pNHEJ-RPG

Plasmid

#85931

Purpose

Constitutively express DsRed and conditionally express Puro and EGFP. Used to test transfection efficiency and enrich genetically modified cells

Depositor

Zhiying Zhang

Article

Ren et al Cell Mol Life Sci. 2015 Jul;72(14):2763-

Insert

pPB-CMV-DsRed-polyA-CAG-NHEJ. Puro-T2A-EGFP

Use

Dual-reporter surrogate

Expression

Mammalian

Available Since

Feb. 10, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3 Tat K41A HA (P#37)

Plasmid

#14655

Depositor

Matija Peterlin

Article

Cujec et al Genes Dev. 1997 Oct 15. 11(20):2645-57.

Insert

Tat (tat HIV)

Tags

HA

Expression

Mammalian

Mutation

K41A

Available Since

June 30, 2008

Availability

Academic Institutions and Nonprofits only
FLAG-ELL pBacPAK8

Plasmid

#15322

Depositor

Joan Conaway, Ronald Conaway

Article

Kong et al Proc Natl Acad Sci U S A. 2005 Jul 19. 1

Insert

eleven nineteen lysine rich in leukemia (ELL Human)

Use

Baculovirus construction

Tags

FLAG

Available Since

Aug. 15, 2007

Availability

Academic Institutions and Nonprofits only
CAG-Brn2-DBD-EnR

Plasmid

#19714

Depositor

Connie Cepko

Article

Kim et al J Neurosci. 2008 Jul 30. 28(31):7748-64.

Insert

Fly engrailed 36240 (Pou3f2, en Fly, Mouse)

Expression

Mammalian

Mutation

mouse Brn2 DNA binding domain-Drosophila engraile…

Available Since

Oct. 27, 2008

Availability

Academic Institutions and Nonprofits only
NTS-PC1

Plasmid

#41574

Depositor

Thomas Weimbs

Article

Talbot et al Proc Natl Acad Sci U S A. 2011 May 10;10

Insert

PKD1 (PKD1 Human)

Tags

CD16.7 and Myc/Myc/His

Expression

Mammalian

Available Since

March 28, 2013

Availability

Academic Institutions and Nonprofits only