Purpose
Expresses SpCas9 and two sgRNA targeting the lenti-CDDR reporter at two distal sites
Inserts
Cas9
sgRNA-A
sgRNA-B
Puromycin resistance
Use
CRISPR
Tags
3xFLAG, Nucleoplasmin NLS, and SV40 NLS
Expression
Mammalian
Mutation
Promoter
CBh, PGK, and U6
Available since
Oct. 28, 2020
Availability
Academic Institutions and Nonprofits only