We narrowed to 2,743 results for: buc

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pENTR-AmCyan

Plasmid

#138481

Purpose

For cloning sgRNA flanked by two ribozyme elements, to subsequently cloned into PL-5LTR-GW-A vector.

Depositor

Akitsu Hotta

Article

Gee et al Nat Commun. 2020 Mar 13;11(1):1334. doi:

Insert

AmCyan

Expression

Mammalian

Available Since

March 23, 2020

Availability

Academic Institutions and Nonprofits only

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FKBP:FRB-FBAR

Plasmid

#223620

Purpose

Transient expression of human FBP17 with mGFP, FRB, FKBP and TagBFP tags in mammalian cells

Depositor

Enfu Hui

Article

Zhao et al Immunity. 2023 Jun 13;56(6):1187-1203.e1

Insert

FBP17 (FNBP1 Human)

Tags

FKBP, TagBFP and mGFP, FRB

Expression

Mammalian

Available Since

Sept. 3, 2024

Availability

Academic Institutions and Nonprofits only

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pHR-SNAP-PDL1

Plasmid

#223602

Purpose

Lentiviral expression of human PD-L1 with SNAP tag in mammalian cells

Depositor

Enfu Hui

Article

Zhao et al Immunity. 2023 Jun 13;56(6):1187-1203.e1

Insert

PD-L1 (CD274 Human)

Use

Lentiviral; mammalian expression

Tags

SNAP

Available Since

Sept. 3, 2024

Availability

Academic Institutions and Nonprofits only

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pHR-CD80-mTFP1

Plasmid

#223605

Purpose

Lentiviral expression of human CD80 with mTFP1 tag in mammalian cells

Depositor

Enfu Hui

Article

Zhao et al Immunity. 2023 Jun 13;56(6):1187-1203.e1

Insert

CD80 (CD80 Human)

Use

Lentiviral; mammalian expression

Tags

mTFP1

Available Since

Sept. 3, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA-mGFP-Eps15DN

Plasmid

#223621

Purpose

Transient expression of human Eps15 with mGFP tag in mammalian cells

Depositor

Enfu Hui

Article

Zhao et al Immunity. 2023 Jun 13;56(6):1187-1203.e1

Insert

Eps15 (EPS15 Human)

Tags

mGFP

Expression

Mammalian

Mutation

Eps15 _ 95-295aa

Available Since

Sept. 3, 2024

Availability

Academic Institutions and Nonprofits only

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pHR-CD86-mTFP1

Plasmid

#223606

Purpose

Lentiviral expression of human CD86 with mTFP1 tag in mammalian cells

Depositor

Enfu Hui

Article

Zhao et al Immunity. 2023 Jun 13;56(6):1187-1203.e1

Insert

CD86 (CD86 Human)

Use

Lentiviral; mammalian expression

Tags

mTFP1

Available Since

Sept. 3, 2024

Availability

Academic Institutions and Nonprofits only

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CL20_mEGFP_ZCCHC7_PAX5

Plasmid

#205958

Purpose

Express mEGFP-tagged fusion protein, ZCCHC7_PAX5 from patient-derived sequence

Depositor

Richard Kriwacki

Article

Tripathi et al Nat Commun. 2023 Sep 28;14(1):6008. doi:

Insert

ZCCHC7_PAX5 (ZCCHC7, PAX5 Human)

Tags

mEGFP

Expression

Mammalian

Available Since

Nov. 9, 2023

Availability

Academic Institutions and Nonprofits only

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pKLV2-U6gRNA5(SMARCA2(47))-PGKpuro2ABFP-W

Plasmid

#200510

Purpose

Lentiviral vector expressing gRNA targeting human SMARCA2

Depositor

Kosuke Yusa

Article

Aoki et al Blood. 2023 Nov 3:blood.2023020857. doi:

Insert

SMARCA2(47) (SMARCA2 Human)

Use

Lentiviral

Available Since

Jan. 18, 2024

Availability

Academic Institutions and Nonprofits only

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YA1529: pAAV_hSyn-QuasAr3-P2A-CheRiff

Plasmid

#107700

Purpose

in vivo voltage imaging

Depositor

Adam Cohen

Article

Adam et al Nature. 2019 May 1. pii: 10.1038/s41586-

Insert

QuasAr3-P2A-CheRiff

Use

AAV

Expression

Mammalian

Promoter

human synapsin I

Available Since

May 2, 2019

Availability

Academic Institutions and Nonprofits only

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pENTR-3xFLAG-YAP1-S127A

Plasmid

#42239

Depositor

William Pu

Article

von Gise et al Proc Natl Acad Sci U S A. 2012 Feb 14;10

Insert

YAP1 (YAP1 Human)

Use

Gateway

Tags

3xFLAG

Mutation

Serine 127 changed to alanine

Promoter

none

Available Since

May 7, 2013

Availability

Academic Institutions and Nonprofits only

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SGyA

Plasmid

#160292

Purpose

Drives expression of Cas9 and a TdTomato fluorescent marker

Depositor

Omar Akbari

Article

Gamez et al Nat Commun. 2021 Dec 10;12(1):7202. doi:

Inserts

Vas promoter
hSpCas9
tdTomato
CTCF insulator
Gypsy insulator
Left homology arm Y chromosome
Right homology arm Y chromosome

Tags

T2A GFP

Expression

Insect

Available Since

March 22, 2021

Availability

Academic Institutions and Nonprofits only

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pCMV-Tag4A-MMP8 Wt

Plasmid

#29545

Depositor

Yardena Samuels

Article

Palavalli et al Nat Genet. 2009 May . 41(5):518-20.

Insert

Metalloproteinase protein 8 (MMP8 Human)

Tags

FLAG

Expression

Bacterial and Mammalian

Available Since

May 23, 2011

Availability

Academic Institutions and Nonprofits only
pMSCV-IRES-GFP/CALR wt-FLAG

Plasmid

#204525

Purpose

Retroviral expression of human CALR wt-FLAG

Depositor

Norio Komatsu

Article

Araki et al Blood. 2016 Jan 27. pii: blood-2015-09-6

Insert

CALR wt-FLAG (CALR Human)

Use

Retroviral

Available Since

Dec. 13, 2023

Availability

Academic Institutions and Nonprofits only

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pLKO_Ollas.S.V5_mCherry-NLS

Plasmid

#178211

Purpose

Nuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables spatial detection of CRISPR screens.

Depositor

Brian Brown

Article

Dhainaut et al Cell. 2022 Mar 10. pii: S0092-8674(22)00

Insert

nPC Tagged mCherry-NLS

Use

Lentiviral

Tags

Nuclear Localization Signal and Ollas.S.V5

Promoter

eF1a

Available Since

July 26, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3.2/DEST/hCx43-E2HA-stop

Plasmid

#40909

Depositor

Robin Shaw

Article

Smyth et al Circ Res. 2012 Mar 30;110(7):978-89. Epu

Insert

Connexin 43 internal HA tag (GJA1 Human)

Expression

Mammalian

Mutation

HA tag inserted in second extracellular loop

Promoter

CMV

Available Since

Nov. 21, 2012

Availability

Academic Institutions and Nonprofits only
PL-5LTR-GW-A

Plasmid

#138480

Purpose

For expression of ribozyme-flanked sgRNA after the Gateway (GW) LR cloning.

Depositor

Akitsu Hotta

Article

Gee et al Nat Commun. 2020 Mar 13;11(1):1334. doi:

Insert

Gateway attR1/R2 cassette with chloramphenicol resistance and ccdB genes

Expression

Mammalian

Available Since

March 23, 2020

Availability

Academic Institutions and Nonprofits only

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pYTSK01K_0G7

Plasmid

#177296

Purpose

genomic integration of genes (inserted in I-SceI-site) via transposon Tn7 with GmR

Depositor

Karl-Erich Jaeger

Article

Tiso et al Front Bioeng Biotechnol. 2020 Aug 20;8:9

Insert

PaacC1-aacC1

Use

Synthetic Biology; Yeast expression, tn7 genomic …

Expression

Bacterial

Available Since

Aug. 31, 2024

Availability

Academic Institutions and Nonprofits only

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pGEMHE-mScarlet-hCenpC

Plasmid

#171490

Purpose

T7 promotor drives in vitro transcription of mScarlet-tagged human hCenpC mRNA

Depositor

Melina Schuh

Article

Cavazza et al Cell. 2021 Apr 30. pii: S0092-8674(21)00

Insert

CenpC (CENPC Human)

Use

Pgehme

Tags

mScarlet

Promoter

T7

Available Since

July 21, 2021

Availability

Academic Institutions and Nonprofits only

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CL20_mEGFP_PAX5_NCOA5

Plasmid

#205885

Purpose

Express mEGFP-tagged fusion protein, PAX5_NCOA5 from patient-derived sequence

Depositor

Richard Kriwacki

Article

Tripathi et al Nat Commun. 2023 Sep 28;14(1):6008. doi:

Insert

PAX5_NCOA5 (PAX5, NCOA5 Human)

Tags

mEGFP

Expression

Mammalian

Available Since

Nov. 9, 2023

Availability

Academic Institutions and Nonprofits only

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YA1532: pAAV_ CAG-FLEX-paQuasAr3

Plasmid

#107702

Purpose

in vivo voltage imaging

Depositor

Adam Cohen

Article

Adam et al Nature. 2019 May 1. pii: 10.1038/s41586-

Insert

paQuasAr3

Use

AAV

Expression

Mammalian

Available Since

May 2, 2019

Availability

Academic Institutions and Nonprofits only

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