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pFSW Syt1-C2A M173,F234A IRES GFP

Plasmid

#133825

Purpose

Encodes the C2A domain of synaptotagmin 1 with membrane-penetrating residue mutations M173,F234A for viral expression

Depositor

Edwin Chapman

Article

Courtney et al Nat Commun. 2019 Sep 9;10(1):4076. doi:

Insert

Syt1 (Syt1 Rat)

Use

Lentiviral

Tags

IRES-GFP

Expression

Mutation

M173, F234A

Promoter

hSyn (human synapsin I promoter)

Available since

Availability

Academic Institutions and Nonprofits only

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pCIBN(deltaNLS)-pmGFP

Plasmid

#26867

Purpose

Expresses CIBN(1-170, -NLS)-eGFP-CaaX fusion for use in light-inducible protein interaction modules for targeting to the plasma membrane

Depositor

Chandra Tucker

Article

Kennedy et al Nat Methods. 2010 Dec;7(12):973-5. doi:

Insert

CIBN(deltaNLS)-pmGFP (CIB1 Mustard Weed)

Use

Tags

EGFP

Expression

Mammalian

Mutation

NLS sequences mutated

Promoter

CMV

Available since

Feb. 24, 2011

Availability

Academic Institutions and Nonprofits only

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pRSET-6xTR-TUBE

Plasmid

#110313

Purpose

E. coli expression and purification of 6xTR-TUBE

Depositor

Yasushi Saeki

Article

Tsuchiya et al Nat Commun. 2018 Feb 6;9(1):524. doi: 10

Insert

6xTR-TUBE (UBQLN1 Human)

Use

Tags

N-terminal His6-T7 tag

Expression

Bacterial

Mutation

All Arg residues in the UBA domain are mutated to…

Promoter

T7

Available since

May 17, 2018

Availability

Academic Institutions and Nonprofits only

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AAV-hSYN-eGFP

Plasmid

#162512

Purpose

AAV vector plasmid expressing enhanced green fluorescent protein (eGFP) under the human synapsin (hSYN) promoter

Depositor

Bart Nieuwenhuis

Article

Nieuwenhuis et al Gene Ther. 2020 Jun 23. pii: 10.1038/s41

Insert

eGFP

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

hSYN

Available since

April 20, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-TRE-ChR2-YFP

Plasmid

#171622

Purpose

TRE-ChR2-YFP reporter

Depositor

Hyungbae Kwon

Article

Hyun et al Nat Commun. 2022 Dec 12;13(1):7692. doi:

Insert

TRE-ChR2-YFP

Use

AAV and Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

TetO

Available since

Oct. 15, 2021

Availability

Academic Institutions and Nonprofits only

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pLVX-opto-mMLKL

Plasmid

#208784

Purpose

Inducibly expresses mouse MLKL fused to light-activatable Cry2

Depositor

Petr Broz

Article

Shkarina et al J Cell Biol. 2022 Jun 6;221(6). pii: 213

Insert

mCherry mMLKL Cry2 (Mlkl Mouse)

Use

Lentiviral

Tags

Cry2 and mCherry

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 2, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-synP-FLEX-splitTVA-EGFP-B19G

Plasmid

#52473

Purpose

Expresses splitTVA-EGFP-B19G

Depositor

Ian Wickersham

Article

Kohara et al Nat Neurosci. 2014 Feb;17(2):269-79. doi

Has Service

AAV1

Insert

TVA-P2A-EGFP-P2A-B19G

Use

AAV and Cre/Lox

Tags

Expression

Mammalian

Mutation

Promoter

Synapsin-1

Available since

April 14, 2014

Availability

Academic Institutions and Nonprofits only

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Gal4BD-CRY2(535)

Plasmid

#75369

Purpose

Yeast two-hybrid expression vector, expresses Gal4BD fused to Arabidopsis CRY2 (residues 1-535)

Depositor

Chandra Tucker

Article

Taslimi et al Nat Chem Biol. 2016 Apr 11. doi: 10.1038

Insert

CRY2 (CRY2 Mustard Weed)

Use

Tags

Gal4BD

Expression

Yeast

Mutation

Promoter

ADH1

Available since

Aug. 15, 2016

Availability

Academic Institutions and Nonprofits only

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PX552

Plasmid

#60958

Purpose

pAAV-U6sgRNA(SapI)_hSyn-GFP-KASH-bGH (SpGuide acceptor). AAV plasmid for sgRNA cloning. GFP-KASH fusion facilitates FACS sorting of cells and nuclei.

Depositor

Feng Zhang

Article

Swiech et al Nat Biotechnol. 2014 Oct 19. doi: 10.103

Inserts

U6_(SpaI)_sgRNA
Syn_EGFP-KASH

Use

AAV, CRISPR, and Mouse Targeting

Tags

Expression

Mammalian

Mutation

Promoter

Synapsin and U6

Available since

Nov. 17, 2014

Availability

Academic Institutions and Nonprofits only

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TDH3-STE2Δ(301-431)-BFP-CIBN-MKII-eLOV-TEVcs(ENLYFQ/M)LexAVP16-tCYC

Plasmid

#135465

Purpose

Membrane-anchored TF used for yeast directed evolution of TEV protease

Depositor

Alice Ting

Article

Sanchez et al Nat Methods. 2019 Dec 9. pii: 10.1038/s4

Insert

STE2Δ(301-431)-BFP-CIBN-MKII-eLOV-TEVcs(ENLYFQ/M)-LexAVP16

Use

Tags

BFP, flag

Expression

Yeast

Mutation

Promoter

TDH3

Available since

Jan. 14, 2020

Availability

Academic Institutions and Nonprofits only

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pGP-AAV-syn-iGABASnFR2-WPRE

Plasmid

#218874

Purpose

AAV-mediated expression of improved GABA sensor (positive change in fluorescence)

Depositor

GENIE Project

Article

Kolb et al bioRxiv 2025.03.25.644953

Has Service

AAV1 and AAV5

Insert

iGABASnFR2

Use

AAV

Tags

Expression

Mammalian

Mutation

S99A F102Y F104Y L178S

Promoter

Synapsin

Available since

June 18, 2024

Availability

Academic Institutions and Nonprofits only

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EB3N-VVDfast-Halo-iLID

Plasmid

#190166

Purpose

Expresses EB3N-VVDfast-Halo-iLID, a microtubule anchor used for optogenetic recruitment of SspB-mCh-p60, in mammalian cells.

Depositor

Anna Akhmanova

Article

Meiring et al Curr Biol. 2022 Nov 7;32(21):4660-4674.e

Insert

EB3N-VVDfast-Halo-iLID

Use

Tags

-VVDfast-mClover3-iLID

Expression

Mammalian

Mutation

1-189 aa from EB3

Promoter

Available since

Oct. 11, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-Ef1a-DIO-ChETA-TdTomato-WPRE-pA

Plasmid

#37755

Purpose

Cre-dependent expression of ChETA-tdTomato, for optogenetic excitation

Depositor

Bernardo Sabatini

Article

Saunders et al Front Neural Circuits. 2012 Jul 27;6:47.

Insert

ChETA-TdTomato

Use

AAV and Cre/Lox; Cre-on

Tags

TdTomato

Expression

Mutation

Promoter

EF1a

Available since

July 27, 2012

Availability

Academic Institutions and Nonprofits only

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pLVX-opto-hCaspase-1

Plasmid

#208790

Purpose

Inducibly expresses human Caspase1, fused to light-activatable Cry2

Depositor

Petr Broz

Article

Shkarina et al J Cell Biol. 2022 Jun 6;221(6). pii: 213

Insert

Caspase1 (CASP1 Human)

Use

Lentiviral

Tags

Cry2 mCherry

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 2, 2023

Availability

Academic Institutions and Nonprofits only

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pTriEx-mCherry-PA-Rac1

Plasmid

#22027

Purpose

Expression of photoactivatable Rac1

Depositor

Klaus Hahn

Article

Wu et al Nature. 2009 Sep 3. 461(7260):104-8.

Insert

PA-Rac1 (RAC1 Human, Avena sativa (oat))

Use

Tags

6xHis and mCherry

Expression

Bacterial, Insect, and Mamm…

Mutation

LOV(Leu404-Leu546), Rac1 starts at Ile4 and conta…

Promoter

CMV, p10

Available since

Sept. 29, 2009

Availability

Academic Institutions and Nonprofits only

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pAAV-TRE-NpHR-EYFP

Plasmid

#171621

Purpose

TRE-NpHR-EYFP reporter

Depositor

Hyungbae Kwon

Article

Hyun et al Nat Commun. 2022 Dec 12;13(1):7692. doi:

Insert

TRE-NpHR-EYFP

Use

AAV and Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

TetO

Available since

Oct. 15, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1a-DIO-hChR2(H134R)-P2A-EYFP

Plasmid

#139283

Purpose

Express hChR2(H134R)-P2A-EYFP in a Cre-dependent manner

Depositor

Mingshan Xue

Article

Chen et al Elife. 2020 Feb 19;9. pii: 48705. doi: 1

Insert

hChR2(H134R)-P2A-EYFP

Use

AAV

Tags

Expression

Mutation

Promoter

Available since

March 24, 2020

Availability

Academic Institutions and Nonprofits only

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CRY2(535, L348F)-mCherry

Plasmid

#75372

Purpose

Expresses fusion of Arabidopsis CRY2 (residues 1-535, L348F)-mCherry

Depositor

Chandra Tucker

Article

Taslimi et al Nat Chem Biol. 2016 Apr 11. doi: 10.1038

Insert

CRY2 residues 1-535 (CRY2 Mustard Weed)

Use

Tags

mCherry

Expression

Mammalian

Mutation

L348F

Promoter

CMV

Available since

Aug. 15, 2016

Availability

Academic Institutions and Nonprofits only

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CRY2PHR(W349R)-mCherry

Plasmid

#75370

Purpose

Expresses fusion of Arabidopsis CRY2 (residues 1-498, W349R fast-cycling mutant) fused to mCherry

Depositor

Chandra Tucker

Article

Taslimi et al Nat Chem Biol. 2016 Apr 11. doi: 10.1038

Insert

CRY2 residues 1-498 (CRY2 Mustard Weed)

Use

Tags

mCherry

Expression

Mammalian

Mutation

W349R

Promoter

CMV

Available since

Aug. 15, 2016

Availability

Academic Institutions and Nonprofits only

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Stargazin-GFP-LOVpep

Plasmid

#80406

Purpose

Membrane localized GFP-tagged LOVpep. Expresses in mammalian cells

Depositor

Michael Glotzer

Article

Wagner et al J Cell Biol. 2016 Jun 20;213(6):641-9. d

Insert

Stargazin-GFP-LOVpep (CACNG2 Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 1, 2016

Availability

Academic Institutions and Nonprofits only

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