We narrowed to 23,974 results for: nes

1. CeNL_pRSETb

   Plasmid

   #89510

   Purpose

   Cyan color variant of bright luminescent protein for bacterial expression

   Depositor

   Takeharu Nagai

   Article

   Suzuki et al Nat Commun. 2016 Dec 14;7:13718. doi: 10

   Insert

   CeNL
   

   Use

   Tags

   His X 6

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   April 19, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pX330S-4

   Plasmid

   #58780

   Purpose

   Expresses Cas9 nuclease and gRNA

   Depositor

   Takashi Yamamoto

   Article

   Sakuma et al Sci Rep. 2014 Jun 23;4:5400. doi: 10.103

   Insert

   humanized S. pyogenes Cas9 nuclease
   

   Use

   CRISPR

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CBh

   Available since

   Nov. 7, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pFZ19

   Plasmid

   #36184

   Depositor

   Daniel Voytas

   Article

   Zhang et al Proc Natl Acad Sci U S A. 2010 Jun 29. 1

   Type

   Empty backbone

   Use

   Plant t-dna vector

   Tags

   Expression

   Plant

   Mutation

   Promoter

   XVE

   Available since

   Aug. 24, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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4. pJSK663

   Plasmid

   #205129

   Purpose

   Expression of GFP-1xPX (from SnxA) in mammalian cells

   Depositor

   Jason King

   Article

   Vines et al J Cell Biol. 2023 Sep 4;222(9):e20220907

   Insert

   1xPX domain of SnxA
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. CeNL/pcDNA3

   Plasmid

   #85199

   Purpose

   Cyan color variant of bright luminescent protein

   Depositor

   Takeharu Nagai

   Article

   Suzuki et al Nat Commun. 2016 Dec 14;7:13718. doi: 10

   Insert

   Cyan enhanced Nano-lantern
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Dec. 7, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. MSGV Hu Acceptor

   Plasmid

   #64269

   Purpose

   Retroviral golden gate cloning vector for expression of functional Human T-Cell Receptor genes

   Depositor

   David Ott

   Article

   Coren et al Biotechniques. 2015 Mar 1;58(3):135-9. d

   Type

   Empty backbone

   Use

   Retroviral

   Tags

   none

   Expression

   Mutation

   Promoter

   Available since

   April 29, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. ReNL_pRSETb

   Plasmid

   #89536

   Purpose

   Red color variant of bright luminescent protein for bacterial expression

   Depositor

   Takeharu Nagai

   Article

   Suzuki et al Nat Commun. 2016 Dec 14;7:13718. doi: 10

   Insert

   ReNL
   

   Use

   Tags

   His X 6

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   May 9, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pX330A-1x3

   Plasmid

   #58767

   Purpose

   Expresses Cas9 nuclease and gRNA

   Depositor

   Takashi Yamamoto

   Article

   Sakuma et al Sci Rep. 2014 Jun 23;4:5400. doi: 10.103

   Insert

   humanized S. pyogenes Cas9 nuclease
   

   Use

   CRISPR

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CBh

   Available since

   Nov. 7, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pcDNA3.1-Orange_CaMBI_300

   Plasmid

   #124095

   Purpose

   orange calcium-modulated bioluminescent indicator, 300nM

   Depositor

   Michael Lin

   Article

   Oh et al Nat Chem Biol. 2019 Apr 1. pii: 10.1038/

   Insert

   Orange CaMBI 300
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 3, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. RP4-11

    Plasmid

    #79812

    Purpose

    Modified RP4 plasmid with Km resistance and sacB gene. Easily curable helper plasmid

    Depositor

    Michael Hynes

    Article

    Quandt et al Plasmid. 2004 Jul;52(1):1-12.

    Insert

    Kanamycin cassette and SacB gene (sacB )
    

    Use

    Helper plasmid for mobilization

    Tags

    Expression

    Mutation

    Promoter

    Km

    Available since

    Aug. 19, 2016

    Availability

    Academic Institutions and Nonprofits only

11. pLX302 LacZ-V5 puro

    Plasmid

    #98579

    Purpose

    Lentiviral vector for constitutive expression of human LacZ with C-terminal V5 tag

    Depositor

    Kevin Janes

    Article

    Bajikar et al Dev Cell. 2017 Nov 20;43(4):418-435.e13.

    Insert

    LacZ
    

    Use

    Lentiviral

    Tags

    2x V5 tag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 15, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. OeNL/pcDNA3

    Plasmid

    #85202

    Purpose

    Orange color variant of bright luminescent protein

    Depositor

    Takeharu Nagai

    Article

    Suzuki et al Nat Commun. 2016 Dec 14;7:13718. doi: 10

    Insert

    Orange enhanced Nano-lantern
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 7, 2016

    Availability

    Academic Institutions and Nonprofits only

13. GeNL(Ca2+)_520/pcDNA3

    Plasmid

    #85204

    Purpose

    luminescent reporter for calcium signaling

    Depositor

    Takeharu Nagai

    Article

    Suzuki et al Nat Commun. 2016 Dec 14;7:13718. doi: 10

    Insert

    GeNL(Ca2+)_520
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pX330S-5

    Plasmid

    #58781

    Purpose

    Expresses Cas9 nuclease and gRNA

    Depositor

    Takashi Yamamoto

    Article

    Sakuma et al Sci Rep. 2014 Jun 23;4:5400. doi: 10.103

    Insert

    humanized S. pyogenes Cas9 nuclease
    

    Use

    CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CBh

    Available since

    Nov. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pcDNA3.1-Blue_CaMBI

    Plasmid

    #124097

    Purpose

    Blue calcium-modulated bioluminescent indicator

    Depositor

    Michael Lin

    Article

    Oh et al Nat Chem Biol. 2019 Apr 1. pii: 10.1038/

    Insert

    Blue CaMBI
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. GeNL(Ca2+)_60/pcDNA3

    Plasmid

    #85207

    Purpose

    luminescent reporter for calcium signaling

    Depositor

    Takeharu Nagai

    Article

    Suzuki et al Nat Commun. 2016 Dec 14;7:13718. doi: 10

    Insert

    GeNL(Ca2+)_60
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pcDNA3.1-Green_CaMBI

    Plasmid

    #124096

    Purpose

    Green calcium-modulated bioluminescent indicator

    Depositor

    Michael Lin

    Article

    Oh et al Nat Chem Biol. 2019 Apr 1. pii: 10.1038/

    Insert

    Green CaMBI
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pHCMV_JEV_prME

    Plasmid

    #207283

    Purpose

    Mammalian expression of Japanese encephalitis virus PrME protein

    Depositor

    Feng Zhang

    Article

    Strebinger et al Nat Commun. 2023 Aug 23;14(1):5141. doi:

    Insert

    JEV_PrME (POLY Japanese encephalitis virus)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    mammalian codon optimization

    Promoter

    CMV promoter

    Available since

    Nov. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pX330S-6

    Plasmid

    #58782

    Purpose

    Expresses Cas9 nuclease and gRNA

    Depositor

    Takashi Yamamoto

    Article

    Sakuma et al Sci Rep. 2014 Jun 23;4:5400. doi: 10.103

    Insert

    humanized S. pyogenes Cas9 nuclease
    

    Use

    CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CBh

    Available since

    Nov. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. YeNL_pRSETb

    Plasmid

    #89520

    Purpose

    Yellow color variant of bright luminescent protein for bacterial expression

    Depositor

    Takeharu Nagai

    Article

    Suzuki et al Nat Commun. 2016 Dec 14;7:13718. doi: 10

    Insert

    YeNL
    

    Use

    Tags

    His X 6

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    May 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pSIM24 KanR

    Plasmid

    #67583

    Purpose

    Plant Gene Expression Vector

    Depositor

    Indu Maiti

    Article

    Sahoo et al PLoS One. 2014 Jun 4;9(6):e98988. doi: 1

    Type

    Empty backbone

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    M24

    Available since

    July 29, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pAAV-hSyn-BRIC

    Plasmid

    #172341

    Purpose

    AAV transfer plasmid for BRIC (Bioluminescent Red Indicator for Calcium)

    Depositor

    Huiwang Ai

    Article

    Tian et al Nat Commun. 2022 Jul 8;13(1):3967. doi:

    Insert

    BRIC
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    hSyn promoter

    Available since

    Dec. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pDRFLIP38

    Plasmid

    #65516

    Purpose

    FRET biosensor Destination vector for yeast expression. Encodes a FRET pair for fusion with ligand binding domain of interest.

    Depositor

    Wolf Frommer

    Article

    Jones et al eLife. 2014 Apr 15;3:e01741. doi: 10.755

    Type

    Empty backbone

    Use

    Tags

    6His-edCit-attR1 and attR2-edCer-cMyc

    Expression

    Yeast

    Mutation

    Promoter

    pPMA1

    Available since

    Oct. 19, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pMaCTag-13

    Plasmid

    #119992

    Purpose

    Template for C-terminal PCR tagging of mammalian genes (without selection) with mCherry

    Depositor

    Michael Knop

    Article

    Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

    Insert

    mCherry
    

    Use

    Pcr template

    Tags

    mCherry

    Expression

    Mutation

    Promoter

    None

    Available since

    Jan. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pLX-AS

    Plasmid

    #188870

    Purpose

    Compact T-DNA binary vector of the pLX series suitable for assembly of plant virus infectious clones and their Agrobacterium-mediated delivery to plants

    Depositor

    Fabio Pasin

    Article

    Pasin et al STAR Protoc. 2022 Dec 16;3(4):101716. do

    Insert

    none
    

    Use

    Synthetic Biology; Binary t-dna vector

    Tags

    Expression

    Plant

    Mutation

    Promoter

    cauliflower mosaic virus (CaMV) 35S promoter

    Available since

    Nov. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pLV hUbC- VP64-dCas9-VP64-T2A- GFP(No LoxP sites)

    Plasmid

    #66708

    Purpose

    expresses VP64-dCas9-VP64, same plasmid as Addgene 59791 but with LoxP sites removed

    Depositor

    Charles Gersbach

    Insert

    S.Pyogenes VP64-dCas9-VP64
    

    Use

    Lentiviral and Synthetic Biology

    Tags

    VP64

    Expression

    Mutation

    D10A and H840A

    Promoter

    hUbC

    Available since

    April 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pcDNA3.1(+)/Puro/TagRFP-LL5b

    Plasmid

    #112828

    Purpose

    transient/stable overexpression

    Depositor

    Tomasz Prószyński, Joshua Sanes

    Article

    Proszynski et al J Cell Sci. 2013 May 15;126(Pt 10):2225-

    Insert

    LL5b (Phldb2 Mouse)
    

    Use

    Tags

    TagRFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pCAG-RFP-p2a-postASAP

    Plasmid

    #178793

    Purpose

    Expresses voltage sensor targeted to dendrites and spines along with RFP

    Depositor

    Rafael Yuste

    Article

    Cornejo et al Science. 2022 Jan 7;375(6576):82-86. doi

    Insert

    FingR.PSD95, ASAP2f and TagRFP
    

    Use

    Tags

    TagRFP

    Expression

    Mammalian

    Mutation

    mutations in amino acids L146G, S147T N149R, S150…

    Promoter

    CAG

    Available since

    April 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pcDNA3-cdc2-AF (718)

    Plasmid

    #39872

    Depositor

    Jonathon Pines

    Article

    Hagting et al EMBO J. 1998 Jul 15;17(14):4127-38.

    Insert

    Cdc2 (CDK1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    AF = T14A, Y15F

    Promoter

    CMV

    Available since

    June 8, 2015

    Availability

    Academic Institutions and Nonprofits only

30. pEC2735 (pSpy0C2)

    Plasmid

    #220279

    Purpose

    integrative plasmid for heterologous gene expression in S. pyogenes SF370, integrates into the amyA locus via homologous recombination

    Depositor

    Emmanuelle Charpentier

    Article

    Lautenschlager et al Front Bioeng Biotechnol. 2024 Jun 7;12:1

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge