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Plasmid#192201PurposeRetro-HER2CARDepositorInsertRetro-HER2CAR (ERBB2 Synthetic)
UseRetroviral; Mammalian expressionTagsExpressionMutationNAPromoterAvailable sinceNov. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
TRIPZ-bioGEF-UBnc-PURO
Plasmid#208044PurposeEnables inducible expression of low-affinity Avitag-UBnc; to perform bioE3; nc = non-cleavable mutation; selection with puromycinDepositorInsertUBnc (UBC Human)
UseLentiviralTagsLow-affinity AvitagExpressionMutationL73P Mutation near C-terminus suppresses cleavage…PromotertetO/UBCAvailable sinceNov. 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
pKLV2-U6gRNA5(BbsI)-PGKpuro2AmCherry-W
Plasmid#67977PurposeCRISPR gRNA expression vector with an improved scaffold and puro/mCherry markersDepositorInsertU6gRNA cassette, PGKpuro2AmCherry cassette, WPRE
UseLentiviralTagsExpressionMutationDeleted BbsI site within WPREPromoterhuman U6 promoter, mouse PGK promoterAvailable sinceSept. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
pKLV2-EF1a-Cas9Bsd-W
Plasmid#68343PurposeLentiviral vector expressing Cas9 fused with the Blasticidin resistant gene at the C-terminusDepositorInsertEF1a-Cas9 cassette, WPRE
UseCRISPR and LentiviralTagsCas9 fused with Flag-tag and a NLS at the N termi…ExpressionMutationPromoterEF1aAvailable sinceSept. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDONR223_ATG16L1_WT
Plasmid#82255PurposeGateway Donor vector containing ATG16L1 , part of the Target Accelerator Plasmid Collection.DepositorInsertATG16L1 (ATG16L1 Human)
UseGateway entry vectorTagsExpressionMutationWTPromoterNoneAvailable sinceApril 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pKLV2-U6gRNA5(Empty)-PGKBFP2AGFP-W
Plasmid#67979PurposeCas9 activity reporter (control) with BFP and GFP.DepositorInsertU6gRNA cassette, PGKBFP2AGFP cassette, WPRE
UseLentiviralTagsExpressionMutationDeleted BbsI site within WPREPromoterhuman U6 promoter, mouse PGK promoterAvailable sinceSept. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
18065-M03-412
Plasmid#225666PurposeLentivirus protein expression. Control plasmid.DepositorInsertsUseLentiviralTags3xFLAGExpressionMutationC-terminally truncated (aa 1-333 only)PromoterWPRE-SV40p and mCMVpAvailable sinceJan. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
pDONR223_RHOA_WT
Plasmid#81739PurposeGateway Donor vector containing RHOA , part of the Target Accelerator Plasmid Collection.DepositorInsertRHOA (RHOA Human)
UseGateway entry vectorTagsExpressionMutationWTPromoterNoneAvailable sinceApril 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDONR223_BRAF_p.V600E
Plasmid#81700PurposeGateway Donor vector containing BRAF, part of the Target Accelerator Plasmid Collection.DepositorInsertBRAF (BRAF Human)
UseGateway entry vectorTagsExpressionMutationV600EPromoterNoneAvailable sinceApril 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDONR223_NRAS_p.Q61R
Plasmid#81652PurposeGateway Donor vector containing NRAS, part of the Target Accelerator Plasmid Collection.DepositorInsertNRAS (NRAS Human)
UseGateway entry vectorTagsExpressionMutationQ61RPromoterNoneAvailable sinceApril 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
CIRTS-FTO-control
Plasmid#216859PurposeCIRTS RNA targeting system for targeted removal of m6A on transcript at the synapse. CIRTS-FTO-Calm3 and scramble gRNA is expressed under human Syn1 and U6 promoter, respectively.DepositorInsertCIRTS-FTO-Calm3-U6-control gRNA
UseLentiviralTagsmCherry2ExpressionMammalianMutationPromoterSyn1, U6Available sinceApril 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pKLV2.2-h7SKgRNA5(SapI)-hU6gRNA5(BbsI)-PGKpuroBFP-W
Plasmid#72666PurposeLentiviral dual CRISPR gRNA expression vector (human 7SK and human U6 promoters)DepositorInserth7SKgRNA cassette, hU6gRNA cassette, PGKpuroBFP cassette, WPRE
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceOct. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
CIRTS-YTHDF2-control
Plasmid#216857PurposeCIRTS RNA targeting system for targeted knockdown of m6A-containing transcript at the synapse. CIRTS-YTHDF2-Calm3 and scramble gRNA is expressed under human Syn1 and U6 promoter, respectively.DepositorInsertCIRTS-YTHDF2-Calm3-U6-control gRNA
UseLentiviralTagsGFPExpressionMammalianMutationPromoterSyn1, U6Available sinceOct. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
AKAP1-hLOV-TEVcs-GAL4bd-VP64-V5
Plasmid#170995PurposeHiLITR transcription factor with AKAP1 N-terminal tmd targeting sequence (mitochondria)DepositorInsertAKAP1(tmd)-NNES-hLOV-TEVcs(ENLYFQ/M)-GAL4bd-VP64-V5 (AKAP1 Synthetic)
UseLentiviral and Synthetic BiologyTagsV5ExpressionMammalianMutationPromoterEf1-alphaAvailable sinceAug. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pKLV2-U6gRNA5(BbsI)-PGKpuro2ABFP-W
Plasmid#67974PurposeCRISPR gRNA expression vector with an improved scaffold and puro/BFP markersDepositorInsertU6gRNA cassette, PGKpuro2ABFP cassette, WPRE
UseLentiviralTagsExpressionMutationDeleted BbsI site within WPREPromoterhuman U6 promoter, mouse PGK promoterAvailable sinceSept. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
pKLV2-U6gRNA5(gGFP)-PGKmCherry2AGFP-W
Plasmid#67982PurposeCas9 activity reporter with mCherry and GFPDepositorInsertU6gRNA cassette, PGKmCherry2AGFP cassette, WPRE
UseCRISPR and LentiviralTagsExpressionMutationDeleted BbsI site within WPREPromoterAvailable sinceNov. 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDONR223_CTNNB1_WT
Plasmid#81734PurposeGateway Donor vector containing CTNNB1 , part of the Target Accelerator Plasmid Collection.DepositorInsertCTNNB1 (CTNNB1 Human)
UseGateway entry vectorTagsExpressionMutationWTPromoterNoneAvailable sinceApril 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCIG-FGF13A
Plasmid#220793PurposeTo express the human FGF13 isoform 1(FGF13A) in mammalian cells together with EGFPDepositorInsertFGF13 (FGF13 Human)
UseTagsExpressionMutationPromoterAvailable sinceJuly 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pKLV2-U6gRNA5(BbsI)-PGKpuro2AZsG-W
Plasmid#67975PurposeCRISPR gRNA expression vector with an improved scaffold and puro/ZsG markersDepositorInsertU6gRNA cassette, PGKpuro2AZsG cassette, WPRE
UseLentiviralTagsExpressionMutationDeleted BbsI site within WPREPromoterhuman U6 promoter, mouse PGK promoterAvailable sinceSept. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDONR223_NRAS_WT
Plasmid#82151PurposeGateway Donor vector containing NRAS , part of the Target Accelerator Plasmid Collection.DepositorInsertNRAS (NRAS Human)
UseGateway entry vectorTagsExpressionMutationWTPromoterNoneAvailable sinceApril 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLL3.7 FLAG-YAP1-TEAD-P-H2B-mCherry
Plasmid#128327PurposeReporter to evaluate YAP1/TEAD-mediated gene transcriptionDepositorInsertsUseLentiviralTagsFLAGExpressionMammalianMutationPromoterAvailable sinceMarch 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDONR223_RHOA_p.Y42C
Plasmid#81306PurposeGateway Donor vector containing RHOA , part of the Target Accelerator Plasmid Collection.DepositorInsertRHOA (RHOA Human)
UseGateway entry vectorTagsExpressionMutationY42CPromoterNoneAvailable sinceApril 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDONR223_WISP2_WT
Plasmid#81905PurposeGateway Donor vector containing WISP2 , part of the Target Accelerator Plasmid Collection.DepositorInsertWISP2 (CCN5 Human)
UseGateway entry vectorTagsExpressionMutationWTPromoterNoneAvailable sinceApril 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLenti-EF-FH-TAZ S89A-ires-blast
Plasmid#52084PurposeLentiviral expression vector for TAZ S89A mutant with N-terminal FLAG and His tagsDepositorInsertTAZ S89A (WWTR1 Human)
UseLentiviralTagsFLAG and HisExpressionMammalianMutationS89APromoterEF1aAvailable sinceApril 22, 2014AvailabilityAcademic Institutions and Nonprofits only -
pRetroX-TetOne-Puro-p62
Plasmid#204548PurposeExpresses p62 in a doxycycline-dependent manner in mammalian cellsDepositorInsertp62 (SQSTM1 Human)
UseRetroviralTagsExpressionMammalianMutationNonePromoterAvailable sinceAug. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pKLV2.2-mU6gRNA5(SapI)-hU6gRNA5(BbsI)-PGKpuroBFP-W
Plasmid#72667PurposeLentiviral dual CRISPR gRNA expression vector (mouse U6 and human U6 promoters)DepositorInsertmU6gRNA cassette, hU6gRNA cassette, PGKpuroBFP cassette, WPRE
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceOct. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
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pDONR223_BRD4_WT
Plasmid#82323PurposeGateway Donor vector containing BRD4 , part of the Target Accelerator Plasmid Collection.DepositorInsertBRD4 (BRD4 Human)
UseGateway entry vectorTagsExpressionMutationP474LPromoterNoneAvailable sinceApril 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDONR223_TGFBR1_WT
Plasmid#82237PurposeGateway Donor vector containing TGFBR1 , part of the Target Accelerator Plasmid Collection.DepositorInsertTGFBR1 (TGFB1 Human)
UseGateway entry vectorTagsExpressionMutationWTPromoterNoneAvailable sinceApril 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pKLV2-EF1a-BsdCas9-W
Plasmid#67978PurposeLentiviral vector expressing Cas9 fused with the Blasticidin resistant gene at the N-terminusDepositorInsertEF1a-Cas9 cassette, WPRE
UseCRISPR and LentiviralTagsCas9 fused with Flag-tag and a NLS at the N termi…ExpressionMutationPromoterEF1aAvailable sinceSept. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDONR223_HRAS_WT
Plasmid#81777PurposeGateway Donor vector containing HRAS , part of the Target Accelerator Plasmid Collection.DepositorInsertHRAS (HRAS Human)
UseGateway entry vectorTagsExpressionMutationWTPromoterNoneAvailable sinceApril 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDONR223_SETDB1_WT
Plasmid#82291PurposeGateway Donor vector containing SETDB1, part of the Target Accelerator Plasmid Collection.DepositorInsertSETDB1 (SETDB1 Human)
UseGateway entry vectorTagsExpressionMutationWTPromoterNoneAvailable sinceApril 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDONR223_CTCF_WT
Plasmid#81789PurposeGateway Donor vector containing CTCF , part of the Target Accelerator Plasmid Collection.DepositorInsertCTCF (CTCF Human)
UseGateway entry vectorTagsExpressionMutationWTPromoterNoneAvailable sinceApril 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pKLV2-U6gRNA5(BbsI)-PGKpuro2AmAG-W
Plasmid#67976PurposeCRISPR gRNA expression vector with an improved scaffold and puro/mAG markersDepositorInsertU6gRNA cassette, PGKpuro2AmAG cassette, WPRE
UseLentiviralTagsExpressionMutationDeleted BbsI site within WPREPromoterhuman U6 promoter, mouse PGK promoterAvailable sinceSept. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1 Sox2 HM a
Plasmid#26353DepositorInsertSox2 shRNA
UseLentiviral and RNAiTagsExpressionMammalianMutationPromoterAvailable sinceSept. 22, 2010AvailabilityAcademic Institutions and Nonprofits only -
pDONR223_STAT3_WT
Plasmid#82235PurposeGateway Donor vector containing STAT3, part of the Target Accelerator Plasmid Collection.DepositorInsertSTAT3 (STAT3 Human)
UseGateway entry vectorTagsExpressionMutationWTPromoterNoneAvailable sinceApril 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
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pDONR223_NOTCH1_ICN
Plasmid#82087PurposeGateway Donor vector containing NOTCH1 , part of the Target Accelerator Plasmid Collection.DepositorInsertNOTCH1 (NOTCH1 Human)
UseGateway entry vectorTagsExpressionMutationICN (AA1761-2556 present)PromoterNoneAvailable sinceApril 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDONR223_SRC_WT
Plasmid#82165PurposeGateway Donor vector containing SRC , part of the Target Accelerator Plasmid Collection.DepositorInsertSRC (SRC Human)
UseGateway entry vectorTagsExpressionMutationWTPromoterNoneAvailable sinceApril 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDONR223_BRAF_p.N581S
Plasmid#81687PurposeGateway Donor vector containing BRAF, part of the Target Accelerator Plasmid Collection.DepositorInsertBRAF (BRAF Human)
UseGateway entry vectorTagsExpressionMutationN581SPromoterNoneAvailable sinceApril 24, 2017AvailabilityAcademic Institutions and Nonprofits only