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We narrowed to 18,759 results for: nls

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Showing: 4081 - 4120 of 18759 results

  7. pSmart-EF1a-Nla-cas3

    Plasmid
    #178881
    Purpose
    Expression of human codon-optimized NlaCas3 with C terminal NLS and HA tag
    Depositor
    Yan Zhang
    Publication
    Tan et al Mol Cell. 2022 Feb 17;82(4):852-867.e5.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    EF1a-NlaCas3c-bGH polyA
    Tags
    HA and NLS
    Expression
    Mammalian
    Promoter
    EF1a
    Available since
    Jan. 18, 2022
    Availability
    Academic Institutions and Nonprofits only
    178881-plasmid-map-sequence-id-351754

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  8. pLenti EF1a mCherry-T2A-VP64-NtcA-2xNLS_SV40

    Plasmid
    #254310
    Purpose
    Constitutive expression of VP64-NtcA-2xNLS_SV40 for use in the functional aKG-ON biosensor. Requires pLenti Neo glnA PCC 6803 reporter gene plasmid. mCherry to monitor NtcA chimera expression.
    Depositor
    Samuel McBrayer
    Publication
    Sternisha et al Science. 2026 Jul 16;393(6808):eadx8675.
    Insert
    VP64-NtcA-2xNLS_SV40
    Use
    Lentiviral and Synthetic Biology
    Tags
    FLAG and FLAG-mCherry-T2A
    Expression
    Mammalian
    Promoter
    EF1a
    Available since
    July 17, 2026
    Availability
    Academic Institutions and Nonprofits only
    254310-plasmid-map-sequence-id-508529

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  10. pv6_BRD4(2)_BRD.2x

    Plasmid
    #260597
    Purpose
    Expresses two copies of the second bromodomain from BAZ2a fused to eGFP including flexible linker and NLS
    Depositor
    Tuncay Baubec
    Insert
    BRD4(2)_BRD.2x (Brd4 Mouse)
    Expression
    Mammalian
    Mutation
    WT
    Promoter
    CAGGS
    Available since
    Oct. 16, 2026
    Availability
    Academic Institutions and Nonprofits only
    260597-plasmid-map-sequence-id-523811

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  11. pCR197

    Plasmid
    #240643
    Purpose
    Expression of the tri-cistronic construct NES-AmNeonGreen-P2A-mTurquoise2-NLS-T2A-mScarlet-I-PTS1 in Exaiptasia diaphana; In vitro transcription of the same construct via SP6 polymerase
    Depositor
    John Pringle
    Inserts
    AIPGENE865 promoter
    SP6 promoter
    AmNeonGreen
    mTurquoise2
    mScarlet-I
    Use
    Gene expression and genomic integration in fish
    Tags
    NES (from Exaiptasia diaphana MEK2), NLS (from SV…
    Mutation
    Codon-optimized for Exaiptasia diaphana, amino ac…
    Available since
    Aug. 15, 2025
    Availability
    Academic Institutions and Nonprofits only
    240643-plasmid-map-sequence-id-482910

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  13. pDRM166 LKB (Luciferase mKate2 Blast)

    Plasmid
    #183502
    Purpose
    Expresses the firefly luciferase gene, the NLS-tagged mKate2 gene, and the blasticidin resistance gene joined by P2A linkers.
    Depositor
    Wayne Tilley
    Publication
    Mustafa et al Oncogene. 2023 Nov 24. doi: 10.1038/s413
    Inserts
    Firefly Luciferase
    mKate2
    BSD
    Use
    Lentiviral and Luciferase
    Tags
    P2A linker and SV40 NLS with GGS linker
    Expression
    Mammalian
    Promoter
    MND, MND (off Luc-P2A), and MND (off Luc-P2A-mKat…
    Available since
    Nov. 30, 2023
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    183502-plasmid-map-sequence-id-362519

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  15. MSP2262

    Plasmid
    #70703
    Purpose
    Bacterial expression plasmid for Sa-dCas9 (D10A/H557A) & sgRNA targeted to site 1: T7-humanSadCas9-NLS-3xFLAG-T7-Sa-sgRNA(84) #1
    Depositor
    Keith Joung
    Publication
    Kleinstiver et al Nat Biotechnol. 2015 Nov 2. doi: 10.1038
    Inserts
    mammalian codon-optimized Staphylococcus aureus dCas9
    SaCas9 sgRNA (+84)
    Use
    CRISPR
    Tags
    NLS-3xFLAG
    Expression
    Bacterial
    Mutation
    D10A & H557A in SaCas9
    Promoter
    T7
    Available since
    Dec. 4, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    70703-plasmid-map-sequence-id-127765

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  16. mPlum-PCNA-19-SV40NLS-4

    Plasmid
    #55994
    Purpose
    Localization: Nucleus, Excitation: 590, Emission: 649
    Depositor
    Michael Davidson
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    PCNA-19 SV40NLS (PCNA Human)
    Tags
    mPlum
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Feb. 27, 2015
    Availability
    Academic Institutions and Nonprofits only

  17. mCitrine-PCNA-19-SV40NLS-4

    Plasmid
    #56564
    Purpose
    Localization: Nucleus, Excitation: 516, Emission: 529
    Depositor
    Michael Davidson
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    PCNA-19 SV40NLS (PCNA Human)
    Tags
    mCitrine
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Dec. 5, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits
    Cited by

  18. GPKAnls

    Plasmid
    #61094
    Purpose
    GFP-tagged PKA catalytic subunit targeted to the nucleus by addition of a nuclear localization signal
    Depositor
    Steven Green
    Publication
    Bok et al J Neurosci. 2003 Feb 1;23(3):777-87.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    pkac2
    Tags
    EGFP
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Jan. 28, 2015
    Availability
    Academic Institutions and Nonprofits only

  19. BPK3258 - human expression plasmid for eSpCas9(1.1)

    Plasmid
    #101176
    Purpose
    Human expression plasmid for SpCas9 eSpCas9(1.1) variant: CMV-T7-hSpCas9-eSpCas9(1.1)(K848A, K1003A, R1060A)-NLS(SV40)-3xFLAG
    Depositor
    Jennifer Doudna, Keith Joung
    Publication
    Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    hSpCas9-eSpCas9(1.1)(K848A/K1003A/R1060A)
    Tags
    3x FLAG and NLS (SV40)
    Expression
    Mammalian
    Mutation
    K848A/K1003A/R1060A
    Promoter
    CMV
    Available since
    Sept. 29, 2017
    Availability
    Academic Institutions and Nonprofits only
    101176-plasmid-map-sequence-id-193999

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  20. CRY-Gal∆DD (B1013)

    Plasmid
    #92035
    Purpose
    Expresses CRY2 (full-length, with endogenous NLS) fused to Gal4 (aa1-65 of Gal4BD), downstream of mCherry-IRES
    Depositor
    Chandra Tucker
    Publication
    Pathak et al Nucleic Acids Res. 2017 Apr 20. doi: 10.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    CRY2 (CRY2 Mustard Weed)
    Expression
    Mammalian
    Mutation
    Fusion of plant CRY2 to Gal4 binding domain (AA1-…
    Available since
    July 25, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    92035-plasmid-map-sequence-id-183927

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  22. pv6_BRD4(1)_BRD.1x_mutant_N140F

    Plasmid
    #260593
    Purpose
    Expresses a binding-deficient mutant of the first bromodomain from BRD4 fused to eGFP including flexible linker and NLS
    Depositor
    Tuncay Baubec
    Insert
    BRD4(1)_BRD.1x_mutant_N140F (Brd4 Mouse)
    Expression
    Mammalian
    Mutation
    N140F
    Promoter
    CAGGS
    Available since
    Oct. 16, 2026
    Availability
    Academic Institutions and Nonprofits only
    260593-plasmid-map-sequence-id-523808

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  23. pv6_BRD4(2)_BRD.1x_mutant_N434F

    Plasmid
    #260594
    Purpose
    Expresses a binding-deficient mutant of the second bromodomain from BRD4 fused to eGFP including flexible linker and NLS
    Depositor
    Tuncay Baubec
    Insert
    BRD4(2)_BRD.1x_mutant_N434F (Brd4 Mouse)
    Expression
    Mammalian
    Mutation
    N434F
    Promoter
    CAGGS
    Available since
    Oct. 16, 2026
    Availability
    Academic Institutions and Nonprofits only
    260594-plasmid-map-sequence-id-523753

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  31. pTUU

    Plasmid
    #159749
    Purpose
    Ubiquitin promoter-controlled expression of 3×FLAG-NLS-zCas9-NLS. Contains gRNA scaffold for insertion of target sequence (U6-26 promoter). Selection of transgenic seeds by mTomato fluoresce.
    Depositor
    Wolfgang Lukowitz
    Publication
    Angulo et al bioRxiv
    Insert
    gRNA scaffold
    Use
    CRISPR
    Expression
    Plant
    Available since
    Oct. 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    159749-plasmid-map-sequence-id-313818

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  32. pCUU

    Plasmid
    #159747
    Purpose
    Ubiquitin promoter-controlled expression of 3×FLAG-NLS-zCas9-NLS. Contains gRNA scaffold for insertion of target sequence (U6-26 promoter). Selection of transgenic seeds by CFP fluoresce.
    Depositor
    Wolfgang Lukowitz
    Publication
    Angulo et al bioRxiv
    Insert
    gRNA scaffold
    Use
    CRISPR
    Expression
    Plant
    Available since
    Oct. 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    159747-plasmid-map-sequence-id-313815

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  33. pCEE

    Plasmid
    #159746
    Purpose
    Egg cell-specific promoter-controlled expression of 3×FLAG-NLS-zCas9-NLS. Contains gRNA scaffold for insertion of target sequence (U6-26 promoter). Selection of transgenic seeds by CFP fluoresce.
    Depositor
    Wolfgang Lukowitz
    Publication
    Angulo et al bioRxiv
    Insert
    gRNA scaffold
    Use
    CRISPR
    Expression
    Plant
    Available since
    Oct. 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    159746-plasmid-map-sequence-id-313813

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  36. hU6-DR_BsmBI_DR-EFS-RfxCas13d-NLS-2A-Puro-WPRE CasRx pre-gRNA backbone

    Plasmid
    #219823
    Purpose
    EF1α-driven expression of RfxCas13d in mammalian cells. For cloning of pre-guide RNAs compatible with RfxCas13d. Contains a 5' direct repeat. Clone using BsmBI. (Adapted from plasmid #138147)
    Depositor
    Philipp Maass
    Insert
    RfxCas13d
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Promoter
    EF1a core promoter
    Available since
    July 31, 2024
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    219823-plasmid-map-sequence-id-441127

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Showing: 4081 - 4120 of 18759 results