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Addgene
Showing: 5101 - 5150 of 11710 results
  1. pcDNA5-miniTurbo-TAF5-∆ex8

    Plasmid
    #209052
    Purpose
    Vector for generating Flp-In cell lines allowing dox-inducible mammalian expression of miniTurbo fused to TAF5 isoform lacking the alternative exon-8 (∆ex8)
    Insert
    TAF5 deltaexon-8
    Use
    Tags
    miniTurbo
    Expression
    Mammalian
    Mutation
    Promoter
    CMV/TO inducible promoter
    Available since
    June 20, 2024
    Availability
    Academic Institutions and Nonprofits only
  2. pcDNA5-3xFLAG-TAF5-FL_WobbleMut

    Plasmid
    #209055
    Purpose
    Vector for generating Flp-In cell lines allowing dox-inducible mammalian expression of 3xFlag-tagged full length TAF5 isoform mutated to be resistant to TAF5 siRNA
    Insert
    TAF5 full length
    Use
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    CMV/TO inducible promoter
    Available since
    June 20, 2024
    Availability
    Academic Institutions and Nonprofits only
  3. pcDNA5-3xFLAG-TAF5-∆ex8_WobbleMut

    Plasmid
    #209056
    Purpose
    Vector for generating Flp-In cell lines allowing dox-inducible mammalian expression of 3xFlag-tagged TAF5 isoform lacking the alternative exon-8 (∆ex8) mutated to be resistant to TAF5 siRNA
    Insert
    TAF5 deltaexon-8
    Use
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    CMV/TO inducible promoter
    Available since
    June 20, 2024
    Availability
    Academic Institutions and Nonprofits only
  4. pcDNA3.1-VcnTS-E1015A-E1021A

    Plasmid
    #213417
    Purpose
    Vinculin tension sensor (VcnTS) with both directionally asymmetric, force strengthening (DAFS) variant point mutations (E1015A and E1021A).
    Depositor
    Insert
    VcnTS-E1015A-E1021A (VCL Chicken, Synthetic)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Mutated vinculin glutamic acid 1015 and 1021 to a…
    Promoter
    CMV
    Available since
    Feb. 15, 2024
    Availability
    Academic Institutions and Nonprofits only
  5. pcDNA3.1-VcnCS-E1015A-E1021A

    Plasmid
    #213414
    Purpose
    Vinculin conformation sensor (VcnCS) with both directionally asymmetric, force strengthening (DAFS) variant point mutations (E1015A and E1021A).
    Depositor
    Insert
    VcnCS-E1015A-E1021A (VCL Chicken, Synthetic)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Mutated vinculin glutamic acid 1015 and 1021 to a…
    Promoter
    CMV
    Available since
    Feb. 15, 2024
    Availability
    Academic Institutions and Nonprofits only
  6. pcDNA3-C17orf53_1-241-mCer

    Plasmid
    #211443
    Purpose
    mammalian expression of the N-terminal amino acids 1-241 of human C17orf53 C-terminally fused to mCerulean
    Depositor
    Insert
    C17orf53 (HROB Human)
    Use
    Tags
    mCerulean
    Expression
    Mammalian
    Mutation
    Deletion of aa 242-646
    Promoter
    Available since
    Jan. 17, 2024
    Availability
    Academic Institutions and Nonprofits only
  7. pcDNA3-C17orf53_1-241-mVen

    Plasmid
    #211444
    Purpose
    mammalian expression of the N-terminal amino acids 1-241 of human C17orf53 C-terminally fused to mVenus
    Depositor
    Insert
    C17orf53 (HROB Human)
    Use
    Tags
    mVenus
    Expression
    Mammalian
    Mutation
    Deletion of aa 242-646
    Promoter
    Available since
    Jan. 16, 2024
    Availability
    Academic Institutions and Nonprofits only
  8. pcDNA3.1-CRISPR-Puro-sgRPL3-56

    Plasmid
    #208979
    Purpose
    pcDNA3.1 based plasmid for transient transfection of sgRNA-cas9. Containing sgRNA targeting human RPL3 (GRCh38.p14_Chr22:39312868-39312887), 56 is a number given by www.e-crisp.org.
    Depositor
    Insert
    sgRNA targeting human RPL3 (ENST00000216146) C-terminal, No.56 (RPL3 Synthetic, Human)
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV and U6 in tandem
    Available since
    Jan. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
  9. pcDNA3.1-CRISPR-Puro-sgRPL3-62

    Plasmid
    #208647
    Purpose
    pcDNA3.1 based plasmid for transient transfection of sgRNA-cas9. Containing sgRNA targeting human RPL3 (GRCh38.p14_Chr22:39312968-39312987), 62 is a number given by www.e-crisp.org.
    Depositor
    Insert
    sgRNA targeting human RPL3 (ENST00000216146) C-terminal, No.62 (RPL3 Synthetic, Human)
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV and U6 in tandem
    Available since
    Jan. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
  10. pcDNA3.1(+)Gα13-125-NLuc

    Plasmid
    #207155
    Purpose
    Part of REGA-SIGN, a NanoBRET-based G protein biosensor. It encodes GNA13 tagged with NanoLuciferase and is meant to be paired with Ggamma1 labeled with LSS-mKATE2
    Insert
    GNA13 (GNA13 Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    NLuc inserted at position F(125)D
    Promoter
    Available since
    Nov. 20, 2023
    Availability
    Academic Institutions and Nonprofits only
  11. pcDNA3.1(+)Gα12-133-NLuc

    Plasmid
    #207156
    Purpose
    Part of REGA-SIGN, a NanoBRET-based G protein biosensor. It encodes GNA12 tagged with NanoLuciferase and is meant to be paired with Ggamma2 labeled with LSS-mKATE2
    Insert
    GNA12 (GNA12 Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    NLuc inserted at position A(133)F
    Promoter
    Available since
    Nov. 20, 2023
    Availability
    Academic Institutions and Nonprofits only
  12. pcDNA3.1(+)GαsS-246-Nluc

    Plasmid
    #207157
    Purpose
    Part of REGA-SIGN, a NanoBRET-based G protein biosensor. It encodes short transcript variant of GNAS tagged with NanoLuciferase and is meant to be paired with Ggamma1 labeled with LSS-mKATE2
    Insert
    GNAS (GNAS Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    NLuc inserted at position D(246)N
    Promoter
    Available since
    Nov. 20, 2023
    Availability
    Academic Institutions and Nonprofits only
Showing: 5101 - 5150 of 11710 results