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Showing: 2041 - 2060 of 8581 results

  1. pJM671 EF1α TC 3xFLAG-FRB-FGFR4-PRS(M)-(GS)-NLS-VP64-ZF6 in TUPV3

    Plasmid
    #161573
    Purpose
    Constitutive expression of TC 3xFLAG-FRB-FGFR4-PRS(M)-(GS)-NLS-VP64-ZF6 under the EF1α promoter
    Depositor
    Joshua Leonard
    Article
    Muldoon et al Sci Adv. 2021 Feb 19;7(8). pii: 7/8/eabe
    Insert
    TC 3xFLAG-FRB-FGFR4-PRS(M)-(GS)-NLS-VP64-ZF6
    Use
    Synthetic Biology
    Tags
    3x-FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    EF1α
    Available since
    Jan. 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    161573-plasmid-map-sequence-id-317930

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  2. pTBL1269 pLenti-CHYRON17-pEF1a(short)-Luc2-P2A-tdTomato-T2A-TdT

    Plasmid
    #163643
    Purpose
    Makes a lentivirus that expresses hgRNA with 17nt spacer and Luc2, tdTomato, and human TdT.
    Depositor
    Chang Liu
    Article
    Loveless et al Nat Chem Biol. 2021 Jun;17(6):739-747. d
    Inserts
    pU6-CHYRON17 hgRNA
    Luc2-P2A-tdTomato-T2A-human TdT (DNTT Human)
    Use
    Lentiviral
    Tags
    Luc2-P2A-tdTomato-T2A
    Expression
    Mutation
    The SpCas9 sgRNA constant region is mutated to ma…
    Promoter
    EFS and pU6
    Available since
    Feb. 18, 2021
    Availability
    Academic Institutions and Nonprofits only
    163643-plasmid-map-sequence-id-325021

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  4. pEF1a_BbsI-gRNA scaffold-EC73-5'-EcoRI-EC73-3'-HDV_pA_pCMV_EGFP-L202S-P2A-mCherry_pA

    Plasmid
    #176461
    Purpose
    Vector for constructing retron Ec73ncRNA-gRNA chimeric RNA (Ec73 rgRNA), inserting spacer sequence by BbsI digestion and inserting donor sequence by EcoRI digestion. EGFP(L202S)-P2A-mCherry driven by CMV
    Depositor
    Hui Yang
    Article
    Kong et al Protein Cell. 2021 Aug 17. pii: 10.1007/
    Insert
    BbsI-gRNA scaffold-Ec73ncRNA-EcoRI-HDV
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1alpha
    Available since
    Oct. 26, 2021
    Availability
    Academic Institutions and Nonprofits only
    176461-plasmid-map-sequence-id-346306

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  5. pJM612 EF1α TC 3xFLAG-FRB-FGFR4-PRS(M)-(GS)-NLS-VP64-ZF1 in TUPV3

    Plasmid
    #161571
    Purpose
    Constitutive expression of TC 3xFLAG-FRB-FGFR4-PRS(M)-(GS)-NLS-VP64-ZF1 under the EF1α promoter
    Depositor
    Joshua Leonard
    Article
    Muldoon et al Sci Adv. 2021 Feb 19;7(8). pii: 7/8/eabe
    Insert
    TC 3xFLAG-FRB-FGFR4-PRS(M)-(GS)-NLS-VP64-ZF1
    Use
    Synthetic Biology
    Tags
    3x-FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    EF1α
    Available since
    Jan. 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    161571-plasmid-map-sequence-id-317637

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  7. pPB-EF1A-HALO-hLRRK2 (LIR1+2 mutant WEVL -> AEVA / WTFI -> ATFA)

    Plasmid
    #229744
    Purpose
    Expresses Halo tagged human LRRK2 with LIR1 and LIR2 point mutations in mammalian cells. Stable expression with piggybac transposase.
    Depositor
    Shawn Ferguson
    Article
    Bentley-DeSousa et al J Cell Biol. 2025 Feb 3;224(2):e20231015
    Insert
    LRRK2 (LRRK2 Human)
    Use
    Tags
    Halo
    Expression
    Mammalian
    Mutation
    LIR1+2 mutant WEVL -> AEVA / WTFI -> ATFA
    Promoter
    EF1A
    Available since
    Feb. 24, 2025
    Availability
    Academic Institutions and Nonprofits only
    229744-plasmid-map-sequence-id-462110

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  8. pHIE323-LPIV Puro-P2A-miRFP720 ZF6x12-C-DsRed-Express2(x6) phEF1α-BlastR

    Plasmid
    #201539
    Purpose
    Landing pad integration vector used to generate ZF6 synTF reporter line (HIE156), assembled with pPD1178 (destination vector), pHIE324-329 (TUPV1-6), pHIE280 (TUPV7), pPD1157 (linker)
    Depositor
    Joshua Leonard
    Article
    Peruzzi et al Nat Commun. 2024 Jul 4;15(1):5618. doi:
    Inserts
    PuromycinR-P2A-miRFP720
    ZF6x12-C-DsRed-Express2(x6)
    BlasticidinR
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    None, ZF6x12(C)_YB_TATA Minimal Promoter, and hEF…
    Available since
    Dec. 19, 2024
    Availability
    Academic Institutions and Nonprofits only
    201539-plasmid-map-sequence-id-401324

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  9. pOTTC1789 - pAAV (flox-stop) mGrid1 390F gRNA A EF1a eGFP-KASH

    Plasmid
    #131684
    Purpose
    An adeno-associated viral vector expressing nuclear envelope-embedded eGFP and a Cre-dependent guide RNA for mGrid1
    Depositor
    Christopher Richie
    Article
    Gantz et al Elife. 2020 Apr 1;9. pii: 56054. doi: 10
    Inserts
    EGFP-KASH
    SpCas9 sgRNA vs mouse GRID1
    Use
    AAV
    Tags
    KASH
    Expression
    Mutation
    Promoter
    EF1a and mU6-LSL (Cre dependent)
    Available since
    June 25, 2024
    Availability
    Academic Institutions and Nonprofits only
    131684-plasmid-map-sequence-id-437146

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  10. pLV-Hygro-EF1a-EGFP-PolB-T2A-Myc-SIRT6(PAMmut-G60A)

    Plasmid
    #176144
    Purpose
    EGFP fused to the N-terminus of POLB, linked by T2A to N-terminus Myc-tagged SIRT6 with the mutation Gly60Ala, a mutation in the PAM site used by SIRT6-KO gRNA1 & a hygromycin resistance cassette
    Depositor
    Robert Sobol
    Article
    Koczor et al Cell Rep. 2021 Nov 2;37(5):109917. doi:
    Insert
    PolB-T2A-SIRT6 (POLB Human)
    Use
    Lentiviral
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    Mutation in Gly60 to Ala, and a mutation in the P…
    Promoter
    EF1A
    Available since
    Jan. 27, 2022
    Availability
    Academic Institutions and Nonprofits only
    176144-plasmid-map-sequence-id-353152

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  11. pLV-Hygro-EF1a-EGFP-PolB-T2A-Myc-SIRT6(PAMmut-R65A)

    Plasmid
    #176143
    Purpose
    EGFP fused the N-terminus of POLB, linked by T2A to N-terminus Myc-tagged SIRT6 with the mutation Arg65Ala, a mutation in the PAM site used by SIRT6-KO gRNA1 & a hygromycin resistance cassette
    Depositor
    Robert Sobol
    Article
    Koczor et al Cell Rep. 2021 Nov 2;37(5):109917. doi:
    Insert
    PolB-T2A-SIRT6 (POLB Human)
    Use
    Lentiviral
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    Mutation in Arg65 to Ala, and a mutation in the P…
    Promoter
    EF1A
    Available since
    Jan. 27, 2022
    Availability
    Academic Institutions and Nonprofits only
    176143-plasmid-map-sequence-id-353151

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  12. pLV-Hygro-EF1a-XRCC1-EGFP-T2A-Myc-POLB(D256A/PAMmut)

    Plasmid
    #176141
    Purpose
    EGFP fused to the C-terminus of XRCC1, linked by T2A to N-terminus Myc-tagged POLB with the mutation Asp256Ala, a mutation in the PAM site used by POLBKO gRNA1 & a hygromycin resistance cassette
    Depositor
    Robert Sobol
    Article
    Koczor et al Cell Rep. 2021 Nov 2;37(5):109917. doi:
    Insert
    XRCC1-T2A-PolB (XRCC1, POLB Human)
    Use
    Lentiviral
    Tags
    EGFP and MYC
    Expression
    Mammalian
    Mutation
    Myc-tagged PolB with mutation in Asp256 to Ala, a…
    Promoter
    EF1A
    Available since
    Jan. 26, 2022
    Availability
    Academic Institutions and Nonprofits only
    176141-plasmid-map-sequence-id-353041

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  14. pJM621 EF1α TC 3xFLAG-FRB-FGFR4-PRS(M)-(GS)-NLS-DsDed-ZF1 in TUPV3

    Plasmid
    #161572
    Purpose
    Constitutive expression of TC 3xFLAG-FRB-FGFR4-PRS(M)-(GS)-NLS-DsDed-ZF1 under the EF1α promoter
    Depositor
    Joshua Leonard
    Article
    Muldoon et al Sci Adv. 2021 Feb 19;7(8). pii: 7/8/eabe
    Insert
    TC 3xFLAG-FRB-FGFR4-PRS(M)-(GS)-NLS-DsDed-ZF1
    Use
    Synthetic Biology
    Tags
    3x-FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    EF1α
    Available since
    Jan. 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    161572-plasmid-map-sequence-id-317932

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  19. Lentiviral click editor (CE1) plasmid - LTR-pEF1a-PCV2-nCas9-EcKlenow (DRR921)

    Plasmid
    #217798
    Purpose
    Lentiviral plasmid to produce the prototypical CE1 construct (PCV2-nCas9-EcKlenow), expressed from the EF1a promoter
    Depositor
    Benjamin Kleinstiver
    Article
    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103
    Insert
    XTEN-nSpCas9-BPNLS-EcKlenow(-exo)-BPNLS
    Use
    CRISPR and Lentiviral
    Tags
    BPNLS
    Expression
    Mammalian
    Mutation
    nSpCas9(H840A); EcKlenow(-exo;D355A/E357A)
    Promoter
    EF1a
    Available since
    Sept. 6, 2024
    Availability
    Academic Institutions and Nonprofits only
    217798-plasmid-map-sequence-id-432865

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Showing: 2041 - 2060 of 8581 results