We narrowed to 8,683 results for: sgRNA

1. pSUI-SynP-CANTsg

   Plasmid

   #154343

   Purpose

   Expression of the sgRNA targeting to the E. coli can gene

   Depositor

   I-Son Ng

   Article

   Tan et al Biotechnol Bioeng. 2020 May 27. doi: 10.

   Insert

   CANT sgRNA
   

   Use

   CRISPR and Synthetic Biology

   Expression

   Bacterial

   Available Since

   July 22, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pSUI-SynP-CAPsg

   Plasmid

   #154344

   Purpose

   Expression of the sgRNA targeting to the E. coli can gene

   Depositor

   I-Son Ng

   Article

   Tan et al Biotechnol Bioeng. 2020 May 27. doi: 10.

   Insert

   CAP sgRNA
   

   Use

   CRISPR and Synthetic Biology

   Expression

   Bacterial

   Available Since

   July 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pJMP2046

   Plasmid

   #160073

   Purpose

   Z. mobilis CRISPRi, promoter A, empty guide, sfGFP

   Depositor

   Jason Peters

   Article

   Banta et al Appl Environ Microbiol. 2020 Nov 10;86(2

   Insert

   empty sgRNA
   

   Expression

   Bacterial

   Availability

   Academic Institutions and Nonprofits only

   

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4. pJK398

   Plasmid

   #155370

   Purpose

   dCas9 sgRNA oscillator v1 (dCRv1) (p15A)

   Depositor

   Pamela Silver

   Article

   Kuo et al Nucleic Acids Res. 2020 Aug 20;48(14):81

   Insert

   dCas9 sgRNA oscillator v1
   

   Use

   CRISPR

   Expression

   Bacterial

   Availability

   Academic Institutions and Nonprofits only

   

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5. pX459-sgAAVS1

   Plasmid

   #184403

   Purpose

   pX459 (sgRNA and Cas9 expressing plasmid, RRID:Addgene_62988) with targeting sequence specific to AAVS1; targeting sequence is: GGGGCCACTAGGGACAGGAT

   Depositor

   Robert Bradley

   Article

   Udy et al Life Sci Alliance. 2021 Dec 8;5(3). pii:

   Insert

   AAVS1-specific sgRNA
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   U6 promoter

   Available Since

   May 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pHAGE-EFS-MCP-3XBFPnls

   Plasmid

   #75384

   Purpose

   MCP-3XBFPnls

   Depositor

   Thoru Pederson

   Article

   Ma et al Nat Biotechnol. 2016 Apr 18. doi: 10.103

   Insert

   MCP
   

   Use

   Lentiviral

   Tags

   3XBFP

   Expression

   Mammalian

   Promoter

   EFS

   Available Since

   May 25, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pDCC6

   Plasmid

   #59985

   Purpose

   Bi-cistronic Drosophila CRISPR/Cas9 vector, contains hsp70>Cas9 and U6>sgRNA cassette

   Depositor

   Peter Duchek

   Article

   Gokcezade et al G3 (Bethesda). 2014 Sep 17. pii: g3.114.

   Inserts

   Cas9
   U6-2>sgRNA
   

   Use

   CRISPR

   Tags

   3xFLAG

   Promoter

   U6-96Ab and hsp70Bb

   Available Since

   Oct. 3, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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8. pHAGE-EFS-PCP-3XGFPnls

   Plasmid

   #75385

   Purpose

   PCP-3XGFPnls

   Depositor

   Thoru Pederson

   Article

   Ma et al Nat Biotechnol. 2016 Apr 18. doi: 10.103

   Insert

   PCP
   

   Use

   Lentiviral

   Tags

   3XsfGFP

   Expression

   Mammalian

   Promoter

   EFS

   Available Since

   May 25, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pSP618

   Plasmid

   #169235

   Purpose

   Spear-ATAC lentiviral backbone - includes a mU6-sgRNA cassette with flanking Nextera sequencing adapters

   Depositor

   William Greenleaf

   Article

   Pierce et al Nat Commun. 2021 May 20;12(1):2969. doi:

   Insert

   mU6-sgRNA
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Available Since

   Aug. 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pMLS597

    Plasmid

    #188884

    Purpose

    dpy-10 sgRNA expression vector

    Depositor

    Erik Jorgensen

    Article

    Schwartz et al PLoS Genet. 2021 Nov 8;17(11):e1009755.

    Insert

    PU6(K09B11.12)::dpy-10 sgRNA (dpy-10 Nematode)
    

    Expression

    Worm

    Available Since

    Aug. 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pX459_gRNA pAS dual_hspCas9

    Plasmid

    #193312

    Purpose

    Cas9 from S. pyogenes and U6-pAS dual sgRNA (V2.0)

    Depositor

    Simon Elsaesser

    Article

    Meineke et al Methods Mol Biol. 2023;2676:169-180. doi

    Insert

    Cas9 from S. pyogenes and U6-pAS dual sgRNA (V2.0)
    

    Use

    CRISPR

    Expression

    Mammalian

    Mutation

    not applicable

    Available Since

    Dec. 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pX459_gRNA-ROSA26_hspCas9

    Plasmid

    #193310

    Purpose

    Cas9 from S. pyogenes and U6 ROSA26 sgRNA (V2.0)

    Depositor

    Simon Elsaesser

    Article

    Meineke et al Methods Mol Biol. 2023;2676:169-180. doi

    Insert

    Cas9 from S. pyogenes and U6 ROSA26 sgRNA (V2.0)
    

    Use

    CRISPR

    Expression

    Mammalian

    Mutation

    not applicable

    Available Since

    Dec. 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pHAGE-EFS-N22p-3XRFPnls

    Plasmid

    #75387

    Purpose

    N22p-3XRFPnls

    Depositor

    Thoru Pederson

    Article

    Ma et al Nat Biotechnol. 2016 Apr 18. doi: 10.103

    Insert

    N22p
    

    Use

    Lentiviral

    Tags

    3XmCherry

    Expression

    Mammalian

    Promoter

    EFS

    Available Since

    May 25, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCR-U6-gRNA-TRE3G-TdTomato

    Plasmid

    #192658

    Purpose

    A plasmid encoding TRE3G sgRNA and TRE3G-promoter-TdTomato

    Depositor

    Jeffrey Miner

    Article

    Omachi et al PLoS One. 2022 Jun 28;17(6):e0270008. do

    Insert

    TdTomato
    

    Expression

    Mammalian

    Promoter

    U6, TRE3G

    Available Since

    Jan. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pKHH030

    Plasmid

    #89358

    Purpose

    hU6 sgRNA cloning vector for CDKO system

    Depositor

    Michael Bassik

    Article

    Han et al Nat Biotechnol. 2017 Mar 20. doi: 10.103

    Insert

    GFP, Puromycin resistance
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Available Since

    April 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pBS-GMR-eya(shRNA)

    Plasmid

    #157991

    Purpose

    pBSII-KS(-)-based vector backbone containing a dominant eye marker for constructing Drosophila CRISPR HDR templates. Marker allows counter selection against vector integration events.

    Depositor

    Greg Beitel

    Article

    Nyberg et al Fly (Austin). 2020 Oct 5. doi: 10.1080/1

    Type

    Empty backbone

    Use

    CRISPR

    Available Since

    Sept. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pV1200

    Plasmid

    #111429

    Purpose

    CaCas9/gRNA Solo entry plasmid for cloning guides - contains stuffer with BsmBI sites - integrates at ENO1 - Recyclable for serial mutagenesis (Sap2-FLP)

    Depositor

    Gerald Fink

    Article

    Vyas et al Sci Adv. 2015;1(3):e1500248.

    Insert

    CaCas9/sgRNA-BsmBI stuffer
    

    Use

    CRISPR

    Expression

    Yeast

    Available Since

    Oct. 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCR-U6-gRNA-miniCMV-TdTomato

    Plasmid

    #192656

    Purpose

    A plasmid encoding miniCMV sgRNA and miniCMV-promoter-TdTomato

    Depositor

    Jeffrey Miner

    Article

    Omachi et al PLoS One. 2022 Jun 28;17(6):e0270008. do

    Insert

    TdTomato
    

    Expression

    Mammalian

    Promoter

    U6, miniCMV

    Available Since

    Dec. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pHAGE-EFS-PCP-3XNeon-nls

    Plasmid

    #75386

    Purpose

    PCP-3XNeon-nls

    Depositor

    Thoru Pederson

    Article

    Ma et al Nat Biotechnol. 2016 Apr 18. doi: 10.103

    Insert

    PCP
    

    Use

    Lentiviral

    Tags

    3XmNeonGreen

    Expression

    Mammalian

    Promoter

    EFS

    Available Since

    April 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pRGE31

    Plasmid

    #50929

    Purpose

    Expressing sgRNA and Cas9 in plants. The sgRNA was controlled by rice snoRNA U3 promoter

    Depositor

    Yinong Yang

    Article

    Xie et al Mol Plant. 2013 Nov;6(6):1975-83. doi: 1

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Plant

    Promoter

    Rice snoRNA U3 and dual 35S promoter

    Available Since

    April 17, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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