162,160 results

1. pCox8a-mScarlet3-H (aka mYongHong)

   Plasmid

   #239410

   Purpose

   Expresses Cox8a-mScarlet3-H in mammalian cells

   Depositor

   Zhifei Fu

   Article

   Xiong et al Nat Methods. 2025 Apr 17. doi: 10.1038/s

   Insert

   Cox8a (COX8A Human)
   

   Use

   Tags

   mScarlet3-H (aka mYongHong)

   Expression

   Mammalian

   Mutation

   M163H in mScarlet3

   Promoter

   CMV

   Available since

   Aug. 11, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLKO5.sgRNA.EFS.GFP

   Plasmid

   #57822

   Purpose

   3rd generation lentiviral Vector for Sp sgRNA delivery without SpCas9, GFP, EFS Promoter driven

   Depositor

   Benjamin Ebert

   Article

   Heckl et al Nat Biotechnol. 2014 Jun 22. doi: 10.103

   Inserts

   sgRNA
   EFS
   eGFP
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 20, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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3. VR124 pETv2 10His-pG-Tn5 (E54K, L372P)

   Plasmid

   #198468

   Purpose

   E. coli protein expression plasmid encoding hyperactive Tn5 transposase fused to protein G, in T7 Express lysY/Iq protein expression strain.

   Depositor

   Viviana Risca

   Article

   Soroczynski et al bioRxiv 2024.07.11.602973

   Insert

   10His-pG-Tn5 (E54K, L372P)
   

   Use

   Tags

   10His-pG

   Expression

   Bacterial

   Mutation

   E54K, L372P

   Promoter

   Consensus φ10 T7 Promoter

   Available since

   April 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pHAGE2 mCherry-Atl3

   Plasmid

   #86680

   Purpose

   Lentivirus to stably express fluorescent protein in mammalian cells

   Depositor

   Tom Rapoport

   Article

   Wang et al Elife. 2016 Sep 13;5. pii: e18605. doi:

   Insert

   ATL3 (ATL3 Human)
   

   Use

   Lentiviral

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 3, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3-mRFP

   Plasmid

   #13032

   Purpose

   Mammalian expression vector for cloning your gene fused to mRFP

   Depositor

   Doug Golenbock

   Has Service

   Cloning Grade DNA

   Insert

   Monomer Red Fluorescent Protein
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 7, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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6. V5-TurboID-NES_pCDNA3

   Plasmid

   #107169

   Purpose

   expresses V5-tagged TurboID in the mammalian cytosol

   Depositor

   Alice Ting

   Article

   Branon et al Nat Biotechnol. 2018 Aug 20. pii: nbt.42

   Insert

   TurboID (BirA mutant)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Q65P, I87V, R118S, E140K, Q141R, S150G, L151P, V1…

   Promoter

   Available since

   May 4, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pJ4M/TDP-43

   Plasmid

   #104480

   Purpose

   bacterial expression of TDP-43

   Depositor

   Nicolas Fawzi

   Article

   Wang et al EMBO J. 2018 Mar 1;37(5). pii: embj.2017

   Insert

   TDP-43 (TARDBP Human)
   

   Use

   Tags

   MBP-6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pH-ePPE

   Plasmid

   #183097

   Purpose

   For plant prime editing in rice plants

   Depositor

   Caixia Gao

   Article

   Zong et al Nat Biotechnol. 2022 Mar 24. doi: 10.103

   Insert

   Nls-nCas9-NC-nls-MLV-Nls
   

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   D10A in SpCas9

   Promoter

   Available since

   April 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. Sec61b-V5-TurboID

   Plasmid

   #166971

   Purpose

   Expresses ER targeted TurboID for secretome biotinylation

   Depositor

   Hyun-Woo Rhee

   Article

   Kim et al Nat Commun. 2021 Sep 1;12(1):5204. doi:

   Insert

   Sec61b-V5-TurboID
   

   Use

   Tags

   V5

   Expression

   Mammalian

   Mutation

   Promoter

   CMV promoter

   Available since

   April 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. dCas9-VPR_P2A_mCherry

    Plasmid

    #154193

    Purpose

    Lentiviral expression plasmid encoding dCas9-VPR and a P2A linked mCherry marker

    Depositor

    Anna Obenauf

    Article

    Umkehrer et al Nat Biotechnol. 2020 Jul 27. pii: 10.103

    Insert

    sp-dCas9-VPR_P2A_mCherry
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    D10A, H840A

    Promoter

    EF1as

    Available since

    Dec. 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pRepCap6

    Plasmid

    #110770

    Purpose

    pRepCap6 contains both rep and cap genes from the AAV6 genome, but lacks the viral terminal repeats (Rutledge et al., 1998). It lacks adenovirus helper functions required for stock production.

    Depositor

    David Russell

    Article

    Rutledge et al J Virol. 1998 Jan;72(1):309-19.

    Insert

    AAV6 cap, AAV6 rep genes
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pENN.AAV.cTNT.PI.eGFP.WPRE.rBG

    Plasmid

    #105543

    Purpose

    AAV expression of EGFP from cTNT promoter

    Depositor

    James M. Wilson

    Has Service

    AAV9

    Insert

    cTNT-PI-EGFP
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    cTNT

    Available since

    Feb. 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pBabe-Kras G12C

    Plasmid

    #58901

    Purpose

    Expresses human Kras with a G12C mutation

    Depositor

    Channing Der

    Insert

    KRAS (KRAS Human)
    

    Use

    Retroviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    G12C mutation

    Promoter

    Available since

    Sept. 15, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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14. 6 x DBE-luc

    Plasmid

    #153160

    Purpose

    Luciferase reporter plasmid for FOXTF

    Depositor

    Stefan Koch

    Article

    Moparthi et al Differentiation. 2020 Sep - Oct;115:30-3

    Insert

    luciferase
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    Promoter

    minP

    Available since

    Aug. 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pHS2002

    Plasmid

    #239832

    Purpose

    Engineered OrufIscB (NovaIscB) omegaRNA mammalian expression

    Depositor

    Feng Zhang

    Article

    Kannan et al Nat Biotechnol. 2025 May 7. doi: 10.1038

    Insert

    NovaIscB omegaRNA scaffold
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Replacement of A12-A57 with GAAA in omegaRNA scaf…

    Promoter

    human U6

    Available since

    July 21, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCDNA3-GFP

    Plasmid

    #74165

    Purpose

    Mammalian expression of EGFP

    Depositor

    Martine Roussel

    Article

    Vo et al Cancer Cell. 2016 Jan 11;29(1):5-16. doi

    Insert

    GFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. rAAV2/MG1.2

    Plasmid

    #184541

    Purpose

    AAV packaging plasmid, expressing Rep2 and AAV-MG1.2 capsid

    Depositor

    Minmin Luo

    Article

    Lin et al Nat Methods. 2022 Jul 25. pii: 10.1038/s

    Insert

    AAV-MG1.2 capsid
    

    Use

    AAV; Helper vector for packaging

    Tags

    Expression

    Mutation

    changes on AAV9 capsid: K449R, Q588T; insertion o…

    Promoter

    Available since

    July 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pENN239_CRISPRcharm_Kv1

    Plasmid

    #220838

    Purpose

    CRISPRcharm Kv1 expression in mammalian cells

    Depositor

    Jonathan Weissman

    Article

    Neumann et al Science. 2024 Jun 28;384(6703):ado7082.

    Insert

    CRISPRcharm Kv1
    

    Use

    CRISPR

    Tags

    P2A-TagBFP

    Expression

    Mammalian

    Mutation

    N/A

    Promoter

    CAG

    Available since

    June 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAAVS1-NDi-CRISPRi (Gen1)

    Plasmid

    #73497

    Purpose

    Dox-inducible CRISPR interference (CRISPRi) knock in construct into the AAVS1 locus with mCherry marker

    Depositor

    Bruce Conklin

    Article

    Mandegar et al Cell Stem Cell. 2016 Apr 7;18(4):541-53.

    Inserts

    dCas9-KRAB-P2A-mCherry
    rtTA
    

    Use

    CRISPR

    Tags

    HA, KRAB, and NLS

    Expression

    Mammalian

    Mutation

    D10A, H840A (catalytically deactivated Cas9)

    Promoter

    CAG and TRE3G

    Available since

    Dec. 7, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. pSLQ4419 pHR (hU6-BsmBI-BsmBI-EFS-PuroR-WPRE)

    Plasmid

    #214875

    Purpose

    Empty lentiviral backbone for guide array cloning

    Depositor

    Stanley Qi

    Article

    Tieu et al Cell. 2024 Feb 29;187(5):1278-1295.e20.

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge