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We narrowed to 7,255 results for: ALP

Showing: 4121 - 4160 of 7255 results

  4. pAAV-CMV-SpCas9D10A nickase

    Plasmid
    #216737
    Purpose
    Expresses SpCas9-D10A nickase from a CMVd1 promoter. For AAV packaging. Derived from pAAV-CMV-SpCas9 (Addgene #113034)
    Depositor
    Vincent Dion
    Article
    Alvaro et al bioRxiv
    Insert
    Cas9-D10A nickase
    Use
    AAV and CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    D10A
    Promoter
    CMVd1
    Available since
    April 24, 2024
    Availability
    Academic Institutions and Nonprofits only
    216737-plasmid-map-sequence-id-430337

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  6. pAAV.hSyn.FLEX.iGluSnFR3.v857.IgK-NGR

    Plasmid
    #196226
    Purpose
    Fluorescent reporter for glutamate, third generation, variant 857 in NGR backbone. iGluSnFR3.v857.NGR
    Depositor
    Kaspar Podgorski
    Article
    Aggarwal et al Nat Methods. 2023 May 4. doi: 10.1038/s4
    Insert
    pAAV hSyn FLEX iGluSnFR3 v857.NGR
    Use
    AAV, Cre/Lox, Mouse Targeting, and Synthetic Biol…
    Tags
    IgK-chain, Myc epi tag, and NGR TM Domain
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Sept. 29, 2023
    Availability
    Academic Institutions and Nonprofits only
    196226-plasmid-map-sequence-id-409162

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  12. pCEP4-PDGFRA-reporter

    Plasmid
    #136456
    Purpose
    Mammalian fluorescent reporter plasmid for PDGFRA signaling.
    Depositor
    Erik Procko
    Article
    Park et al PLoS Pathog. 2020 Jun 19;16(6):e1008647.
    Inserts
    PDGFRalpha (PDGFRA Human)
    Array of serum response elements (SRE) to drive destabilized GFP expression from a minimal promoter.
    Use
    Tags
    Extracellular c-myc epitope tag, Signal peptide f…
    Expression
    Mammalian
    Mutation
    Promoter
    CMV and Minimal promoter with artificial SRE enha…
    Available since
    July 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    136456-plasmid-map-sequence-id-263271

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  16. pLZRS-ER-Rac1-Q61L-shortlinker

    Plasmid
    #128584
    Purpose
    For mammalian cell expression of Q61L mutant murine RAC1 carrying a modified hormone-binding domain of murine ERalpha at the N-terminus. Activation of expressed latent fusion protein induced by 4-OHT
    Depositor
    Julian Downward
    Article
    Lionarons et al Cancer Cell. 2019 Jul 8;36(1):68-83.e9.
    Insert
    Rac1 (Rac1 Mouse)
    Use
    Retroviral
    Tags
    Modified hormone binding domain of ER alpha
    Expression
    Mammalian
    Mutation
    Changed Glutamine61 to Leucine in the RAC1 moiety.
    Promoter
    Available since
    Aug. 13, 2019
    Availability
    Academic Institutions and Nonprofits only
    128584-plasmid-map-sequence-id-254045

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  17. pLZRS-ER-Rac1-P29S-shortlinker

    Plasmid
    #128583
    Purpose
    For mammalian cell expression of P29S mutant murine RAC1 carrying a modified hormone-binding domain of murine ERalpha at the N-terminus. Activation of expressed latent fusion protein induced by 4-OHT
    Depositor
    Julian Downward
    Article
    Lionarons et al Cancer Cell. 2019 Jul 8;36(1):68-83.e9.
    Insert
    Rac1 (Rac1 Mouse)
    Use
    Retroviral
    Tags
    Modified hormone binding domain of ER alpha
    Expression
    Mammalian
    Mutation
    Changed Proline29 to Serine in the RAC1 moiety.
    Promoter
    Available since
    Aug. 13, 2019
    Availability
    Academic Institutions and Nonprofits only
    128583-plasmid-map-sequence-id-253120

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  18. dCas9-CBP HAT

    Plasmid
    #179547
    Purpose
    encodes S. pyogenes dCas9 with c-terminal fusion of human CBP HAT domain (aa 1323-1701) driven by EF-1alpha promoter
    Depositor
    Isaac Hilton
    Article
    Wang et al Nucleic Acids Res. 2022 Aug 12;50(14):78
    Insert
    S. pyogenes dCas9 with c-terminal fusion of human CBP HAT domain (aa 1323-1701) (CREBBP Human)
    Use
    Lentiviral
    Tags
    Flag Tag
    Expression
    Mammalian
    Mutation
    D10A; H840A in S.pyogenes Cas9
    Promoter
    EF-1alpha
    Available since
    Feb. 18, 2022
    Availability
    Academic Institutions and Nonprofits only
    179547-plasmid-map-sequence-id-354768

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  20. pETDuet-TFIIE

    Plasmid
    #171083
    Purpose
    Co-expresses human TFIIE. The resulting plasmid was used to generate a single expression construct encoding 2 subunits, with His-tag on TF2E1.Originally from MN Gonzalez, was submitted with permission
    Depositor
    Joan Conaway
    Article
    He et al Proc Natl Acad Sci U S A. 2021 Sep 28;11
    Inserts
    TF2E1 (GTF2E1 Human)
    TF2E2 (GTF2E2 Human)
    Use
    Tags
    His
    Expression
    Bacterial
    Mutation
    Promoter
    T7 promoter
    Available since
    July 19, 2024
    Availability
    Academic Institutions and Nonprofits only
    171083-plasmid-map-sequence-id-335640

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  28. pLV(gRNA)-CMV-eGFP-U6(sgCTG)

    Plasmid
    #216732
    Purpose
    Contains a eGFP gene along with a U6 promoter driving the sgCTG (target sequence: (CUG)6). Used with the HD iPSC-derived astrocytes
    Depositor
    Vincent Dion
    Article
    Alvaro et al bioRxiv
    Insert
    sgCTG lentiviral guide RNA with eGFP tag
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV and U6
    Available since
    April 24, 2024
    Availability
    Academic Institutions and Nonprofits only
    216732-plasmid-map-sequence-id-430316

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  32. pVRC8400-SARS-CoV-2-S2P-B.1.617.2-AVI

    Plasmid
    #176334
    Purpose
    Expresses SARS-CoV-2 S2P protein from the B.1.617.2 variant with single chain Fc, HRV3C protease cleavage site, and AVI tag
    Depositor
    Peter Kwong
    Article
    Teng et al PLoS One. 2022 May 24;17(5):e0268767. do
    Insert
    SARS-CoV-2-S2P-B.1.617.2 (S )
    Use
    Tags
    AVI, HRV3C, and scFc
    Expression
    Mammalian
    Mutation
    T19R, G142D, del156-157, R158G, L452R, T478K, D61…
    Promoter
    Available since
    March 2, 2022
    Availability
    Academic Institutions and Nonprofits only
    176334-plasmid-map-sequence-id-347146

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  39. pVRC8400-SARS-CoV-2-RBD-BA.2-AVI

    Plasmid
    #184533
    Purpose
    Expresses SARS-CoV-2 RBD domain from the BA.2 variant with single chain Fc, HRV3C protease cleavage site, and AVI tag
    Depositor
    Peter Kwong
    Article
    Teng et al PLoS One. 2022 May 24;17(5):e0268767. do
    Insert
    SARS-CoV-2-RBD-BA.2 (S )
    Use
    Tags
    AVI, HRV3C, and scFc
    Expression
    Mammalian
    Mutation
    G339D, S371F, S373P, S375F, T376A, D405N, R408S, …
    Promoter
    Available since
    May 10, 2022
    Availability
    Academic Institutions and Nonprofits only
    184533-plasmid-map-sequence-id-361466

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Showing: 4121 - 4160 of 7255 results