We narrowed to 29,405 results for: des.2

1. pBiFC-flag-hCaspase 2 FL (C303S)-VN173

   Plasmid

   #49258

   Purpose

   Expresses split fluorescence protein to measure caspase-2 dimerization in mammalian cells.

   Depositor

   Douglas Green

   Article

   Bouchier-Hayes et al Mol Cell. 2009 Sep 24;35(6):830-40. doi:

   Insert

   caspase-2 (CASP2 Human)
   

   Tags

   flag and split Venus N

   Expression

   Mammalian

   Mutation

   C303S

   Promoter

   CMV

   Available Since

   May 6, 2014

   Availability

   Academic Institutions and Nonprofits only

2. pLV-mScarlet-SARS-CoV-2-SL1 alone

   Plasmid

   #191485

   Purpose

   Lentiviral expression of SARS-CoV-2 5_ UTR SL1 alone

   Depositor

   Hao Wu

   Article

   Vora et al Proc Natl Acad Sci U S A. 2022 Mar 1;119

   Insert

   SARS-CoV-2 5' UTR SL1 alone (NEWENTRY Severe acute respiratory syndrome coronavirus 2)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   Nov. 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3.1(+)/Puro/FA/GFP-C--Catulin-2

   Plasmid

   #112842

   Purpose

   transient/stable overexpression of the central domain of Catulin.

   Depositor

   Tomasz Prószyński

   Article

   Gingras et al J Cell Sci. 2016 Mar 1;129(5):898-911. d

   Insert

   Catulin-alpha-1 (Ctnnal1 Mouse)
   

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   C1 domain of Catulin

   Available Since

   Aug. 8, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pRRL-mouse cGAS #2-gRNA-Cas9-Puro

   Plasmid

   #186891

   Purpose

   gRNA targeting mouse cGAS (with Cas9 insert)

   Depositor

   Jonathan Kagan

   Article

   Mosallanejad et al Sci Immunol. 2023 Jan 20;8(79):eabp9765.

   Insert

   Cas9 (Cgas Mouse)
   

   Use

   Lentiviral

   Available Since

   Oct. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3-SARS-CoV-2-S-RBD-8his

   Plasmid

   #145145

   Purpose

   Mammalian expression plasmid for RBD of SARS-CoV-2 protein S (spike) with 8his tag

   Depositor

   Erik Procko

   Article

   Chan et al Science. 2020 Sep 4;369(6508):1261-1265.

   Insert

   Secreted receptor binding domain (RBD; a.a. 333-529) of S protein from SARS-CoV-2 (S SARS coronavirus 2)
   

   Tags

   8his tag

   Expression

   Mammalian

   Mutation

   Codon-optimized for human cell expression.

   Promoter

   CMV

   Available Since

   April 15, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3-SARS-CoV-2-S-RBD-sfGFP

   Plasmid

   #141184

   Purpose

   Mammalian expression plasmid for RBD of SARS-CoV-2 protein S (spike) fused with sfGFP

   Depositor

   Erik Procko

   Article

   Chan et al Science. 2020 Sep 4;369(6508):1261-1265.

   Has Service

   Cloning Grade DNA

   Insert

   S surface glycoprotein [ Severe acute respiratory syndrome coronavirus 2 ] (S SARS coronavirus 2)
   

   Tags

   Signal peptide from influenza HA and Superfolder …

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   April 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3-SARS-CoV-2-S-RBD-Fc

   Plasmid

   #141183

   Purpose

   Mammalian expression plasmid for RBD of SARS-CoV-2 protein S (spike) fused with IgG1-Fc

   Depositor

   Erik Procko

   Article

   Chan et al Science. 2020 Sep 4;369(6508):1261-1265.

   Insert

   Secreted receptor binding domain (RBD; a.a. 333-529) of S protein from SARS-CoV-2 fused to Fc of IgG1 (S SARS coronavirus 2)
   

   Tags

   Fc region of IgG1 (a.a. Asp-221 to Lys-447) and S…

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   April 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET28a 6xHis PreScission SARS-CoV-2 nsp13

   Plasmid

   #159390

   Purpose

   Expresses the SARS-CoV-2 nsp13 protein with an N-terminal His6 tag followed by an HRV 3C/PreScission cleavage site.

   Depositor

   Tarun Kapoor

   Article

   Chen et al Cell. 2020 Sep 17;182(6):1560-1573.e13.

   Insert

   N-terminal His tag PreScission SARS-CoV-2 nsp13, optimized for insect cell expression
   

   Tags

   His6 tag

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Sept. 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCDNA4TO K164A H165A CoV-2 nsp1 3xFLAG

   Plasmid

   #176060

   Purpose

   Expresses K164A K165A CoV2 nsp1 in mammalian cells. This contains a C-terminal 3xFLAG tag fusion to nsp1.

   Depositor

   Britt Glaunsinger

   Article

   Mendez et al Cell Rep. 2021 Oct 19;37(3):109841. doi:

   Insert

   CoV-2 K164A H165A nsp1 (ORF1ab SARS-CoV-2)
   

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   K164A and H165A

   Promoter

   CMV

   Available Since

   Oct. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3.1 SARS-CoV-2 S D614G-trunc

    Plasmid

    #158077

    Purpose

    Expresses SARS-CoV-2 spike protein with a D614G truncated mutation

    Depositor

    Jeremy Luban

    Article

    Yurkovetskiy et al Cell. 2020 Sep 15. pii: S0092-8674(20)31

    Insert

    SARS-CoV-2 spike (S )
    

    Expression

    Mammalian

    Mutation

    D614G

    Available Since

    Nov. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA3.1 SARS-CoV-2 S D614-trunc

    Plasmid

    #158076

    Purpose

    Expresses SARS-CoV-2 spike protein with a truncation

    Depositor

    Jeremy Luban

    Article

    Yurkovetskiy et al Cell. 2020 Sep 15. pii: S0092-8674(20)31

    Insert

    SARS-CoV-2 spike (S )
    

    Expression

    Mammalian

    Available Since

    Nov. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. 3159_pETcon-SARS-CoV-2-RBD-L452R-T478K

    Plasmid

    #184407

    Purpose

    yeast surface display of the SARS-CoV-2 Delta variant RBD

    Depositor

    Jesse Bloom

    Article

    Starr et al Science. 2022 Jun 28:eabo7896. doi: 10.1

    Insert

    SARS-CoV-2 Delta Spike receptor binding domain (RBD) (S SARS-CoV-2 virus)
    

    Tags

    HA and c-Myc

    Expression

    Yeast

    Mutation

    L452R+T478K

    Available Since

    May 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pMT1-13X-gRNA1-2(AtUBI10)-MTAP-LUC

    Plasmid

    #234370

    Purpose

    T-DNA vector for expression of the two protein components of the MoonTag system under the control of the Arabidopsis Ubi10 promoter; Luciferase under control of transactivated promoter by two gRNAs

    Depositor

    Michael Smanski

    Article

    Casas-Mollano et al Nucleic Acids Res. 2023 Jul 21;51(13):70

    Inserts

    NbGP41-sfGFP-VP64-GB1
    Luciferase
    dCas9-13XGP41
    gRNA 1-1 and gRNA 1-2
    

    Use

    Synthetic Biology

    Tags

    GB1, GP41, and sfGFP

    Expression

    Plant

    Promoter

    Arabidopsis U6, Arabidopsis Ubi10, and MTAP1 (syn…

    Available Since

    April 17, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. pMT1-24X-gRNA1-2(AtUBI10)-MTAP-LUC

    Plasmid

    #234372

    Purpose

    T-DNA vector for expression of the two protein components of the MoonTag system under the control of the Arabidopsis Ubi10 promoter; Luciferase under control of transactivated promoter by two gRNAs

    Depositor

    Michael Smanski

    Article

    Casas-Mollano et al Nucleic Acids Res. 2023 Jul 21;51(13):70

    Inserts

    NbGP41-sfGFP-VP64-GB1
    Luciferase
    dCas9-24XGP41
    gRNA 1-1 and gRNA 1-2
    

    Use

    Synthetic Biology

    Tags

    GB1, GP41, and sfGFP

    Expression

    Plant

    Promoter

    Arabidopsis U6, Arabidopsis Ubi10, and MTAP1 (syn…

    Available Since

    April 17, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. pRRL-mouse STING #2-gRNA-Cas9-Puro

    Plasmid

    #186893

    Purpose

    gRNA targeting mouse STING (with Cas9 insert)

    Depositor

    Jonathan Kagan

    Article

    Mosallanejad et al Sci Immunol. 2023 Jan 20;8(79):eabp9765.

    Insert

    Cas9 (Sting1 Mouse)
    

    Use

    Lentiviral

    Available Since

    Oct. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pET-22b(+)_PelB-BN00N0-2-FLAG-His

    Plasmid

    #218045

    Purpose

    RESOLUTE SLC binder expression plasmid

    Depositor

    RESOLUTE Consortium, Giulio Superti-Furga

    Article

    Gelová et al J Mol Biol. 2024 Jun 13:168665. doi: 10.

    Insert

    BN00N0-2
    

    Tags

    1xFLAG 10xHis and PelB

    Expression

    Bacterial

    Available Since

    May 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pET-22b(+)_PelB-BN00A4-2-FLAG-His

    Plasmid

    #218071

    Purpose

    RESOLUTE SLC binder expression plasmid

    Depositor

    RESOLUTE Consortium, Giulio Superti-Furga

    Article

    Gelová et al J Mol Biol. 2024 Jun 13:168665. doi: 10.

    Insert

    BN00A4-2
    

    Tags

    1xFLAG-10xHis and PelB

    Expression

    Bacterial

    Available Since

    May 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pET-22b(+)_PelB-BN00FH-2-FLAG-His

    Plasmid

    #218037

    Purpose

    RESOLUTE SLC binder expression plasmid

    Depositor

    RESOLUTE Consortium, Giulio Superti-Furga

    Article

    Gelová et al J Mol Biol. 2024 Jun 13:168665. doi: 10.

    Insert

    BN00FH-2
    

    Tags

    1xFLAG 10xHis and PelB

    Expression

    Bacterial

    Available Since

    May 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pGHUC w-ERBIN (2-hybrid prey plasmid)

    Plasmid

    #128163

    Purpose

    UV5 promoter drives expression of omega-erbin fusion. Pair with pB1H2 UV5 Zif268-cons as a positive control (turns on HIS3/GFP).

    Depositor

    Marcus Noyes

    Article

    Ichikawa et al ACS Synth Biol. 2019 May 17;8(5):918-928

    Insert

    ERBIN PDZ domain (ERBIN Synthetic)
    

    Use

    Synthetic Biology

    Tags

    FLAG and Omega subunit of RNA polymerase

    Expression

    Bacterial

    Promoter

    UV5

    Available Since

    Jan. 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. SARS-CoV-2 Nucleocapsid-3xHA-IRES-mcherry

    Plasmid

    #173030

    Purpose

    To express SARS-CoV-2 Nucleocapsid protein tagged with 3xHA and in frame with mcherry (NCP-IRES-mcherry) in mammalian cells

    Depositor

    Mohamed Fareh

    Article

    Fareh et al Nat Commun. 2021 Jul 13;12(1):4270. doi:

    Insert

    SARS-CoV-2 Nucleocapsid phosphoprotein
    

    Use

    Lentiviral

    Tags

    3xHA and IRES-mcherry

    Expression

    Mammalian

    Promoter

    Gag (truncated)

    Available Since

    Sept. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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