We narrowed to 5,537 results for: mcs

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pX459-HypaCas9-mR2-RHOA_sgRNA

Plasmid

#183877

Purpose

pX459V2.0-HypaCas9 plasmid with mRuby2 reporter and RHOA sgRNA for N-terminal tagging of RhoA in human cells.

Depositor

Alisa Piekny

Article

Husser et al Open Biol. 2022 Nov;12(11):220247. doi:

Insert

RHOA sgRNA spacer (RHOA Human)

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

May 20, 2022

Availability

Academic Institutions and Nonprofits only

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G1397 DddAtox-N–SaKKH-Cas9(D10A)

Plasmid

#157839

Purpose

expresses split DddAtox-Cas9 construct in mammalian cells

Depositor

David Liu

Article

Mok et al Nature. 2020 Jul 8. pii: 10.1038/s41586-

Insert

G1397 DddAtox-N–SaKKH-Cas9(D10A)–UGI–SV40 NLS

Expression

Mammalian

Available Since

Aug. 4, 2020

Availability

Academic Institutions and Nonprofits only

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EGFP-uTEV1-MAVS-mTA4

Plasmid

#171002

Purpose

HiLITR protease with MAVS [Y536delinsFIVLI] C-terminal tmd targeting information (Mitochondria and ER insertion)

Depositor

Alice Ting

Article

Coukos et al eLife. 2021 Aug 20;10. pii: 69142. doi:

Insert

EGFP-uTEV1-MAVS(tmd)[Y536delinsFIVLI]

Use

Lentiviral and Synthetic Biology

Expression

Mammalian

Mutation

MAVS(tmd)[Y536delinsFIVLI]

Promoter

pTRE-tight (TetOn)

Available Since

Aug. 19, 2021

Availability

Academic Institutions and Nonprofits only

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RK5F-YAP8SA

Plasmid

#125008

Purpose

express in mammalian cell

Depositor

Ruey-Hwa Chen

Article

Tan et al Oncogene. 2019 Jul;38(28):5612-5626. doi

Insert

YAP8SA (YAP1 Human)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

April 1, 2022

Availability

Academic Institutions and Nonprofits only

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Flag Snail 8SA

Plasmid

#16222

Depositor

Mien-Chie Hung

Article

Zhou et al Nat Cell Biol. 2004 Oct . 6(10):931-40.

Insert

Snail (SNAI1 Human)

Tags

Flag

Expression

Mammalian

Mutation

8SA: S97A, S101A, S105A, S108A, S112A, S116A, S12…

Available Since

Nov. 30, 2007

Availability

Academic Institutions and Nonprofits only
pcDNA3 PARL-FLAG-CT S65D+T69D+S70D

Plasmid

#13617

Depositor

Luca Pellegrini

Article

Jeyaraju et al Proc Natl Acad Sci U S A. 2006 Dec 5. 10

Insert

Presenilin-associated rhomboid-like (PARL, PARL Human)

Tags

FLAG

Expression

Mammalian

Mutation

changed Serine 65, Thr 69 and Ser70 to Asp

Available Since

Jan. 12, 2007

Availability

Academic Institutions and Nonprofits only
pET21-pelB-LaG-27

Plasmid

#172752

Purpose

Bacterial expression of HIS-tagged anti-GFP nanobody LaG-27; HIS tag preceded by HRV 3C/PreScission cleavage site.

Depositor

Michael Rout

Article

Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

Insert

LaG-27

Tags

6xHIS and pelB leader for periplasmic secretion

Expression

Bacterial

Promoter

T7

Available Since

Aug. 18, 2021

Availability

Academic Institutions and Nonprofits only

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HP1-ECFP-SNAP

Plasmid

#187966

Purpose

expresses HP1 with C-terminal tags ECFP and SNAP

Depositor

Markus Wahl

Article

Vester et al Commun Biol. 2022 Jul 22;5(1):736. doi:

Insert

HP1 (CBX5 Human)

Tags

ECFP-SNAP

Expression

Mammalian

Promoter

CMV

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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3542 pMIG Bcl-XL mut1

Plasmid

#8791

Depositor

Stanley Korsmeyer

Article

Cheng et al Mol Cell 2001 Sep;8(3):705-11.

Insert

Bcl-XL (BCL2L1 Human)

Use

Retroviral

Expression

Mammalian

Mutation

F131V, D133A

Available Since

Jan. 5, 2006

Availability

Academic Institutions and Nonprofits only
pcDNA3 PARL-FLAG-CT S65A+T69A+S70A

Plasmid

#13616

Depositor

Luca Pellegrini

Article

Jeyaraju et al Proc Natl Acad Sci U S A. 2006 Dec 5. 10

Insert

Presenilin-associated rhomboid-like (PARL, PARL Human)

Tags

FLAG

Expression

Mammalian

Mutation

changed Ser 65, Thr 69 and Ser 70 to Alanine

Available Since

Jan. 12, 2007

Availability

Academic Institutions and Nonprofits only
pET21-pelB-LaG-24

Plasmid

#172750

Purpose

Bacterial expression of HIS-tagged anti-GFP nanobody LaG-24; HIS tag preceded by HRV 3C/PreScission cleavage site.

Depositor

Michael Rout

Article

Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

Insert

LaG-24

Tags

6xHIS and pelB leader for periplasmic secretion

Expression

Bacterial

Promoter

T7

Available Since

Aug. 18, 2021

Availability

Academic Institutions and Nonprofits only

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pET21-pelB-LaG-9

Plasmid

#172742

Purpose

Bacterial expression of HIS-tagged anti-GFP nanobody LaG-9; HIS tag preceded by HRV 3C/PreScission cleavage site.

Depositor

Michael Rout

Article

Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

Insert

LaG-9

Tags

6xHIS and pelB leader for periplasmic secretion

Expression

Bacterial

Promoter

T7

Available Since

Aug. 18, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3_AVPR2-NTEV-TCS-GV-2xHA

Plasmid

#194370

Purpose

Split TEV assays

Depositor

Michael Wehr

Article

Wu et al Biosensors (Basel). 2022 Dec 29;13(1):48

Insert

AVPR2-NTEV-TCS-GV-2xHA (AVPR2 Human, Synthetic)

Tags

2xHA

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 30, 2023

Availability

Academic Institutions and Nonprofits only

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pET21-pelB-LaG-8

Plasmid

#172761

Purpose

Bacterial expression of HIS-tagged anti-GFP nanobody LaG-8; HIS tag preceded by HRV 3C/PreScission cleavage site.

Depositor

Michael Rout

Article

Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

Insert

LaG-8

Tags

6xHIS and pelB leader for periplasmic secretion

Expression

Bacterial

Promoter

T7

Available Since

Aug. 18, 2021

Availability

Academic Institutions and Nonprofits only

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pET21-pelB-LaG-5

Plasmid

#172760

Purpose

Bacterial expression of HIS-tagged anti-GFP nanobody LaG-5; HIS tag preceded by HRV 3C/PreScission cleavage site.

Depositor

Michael Rout

Article

Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

Insert

LaG-5

Tags

6xHIS and pelB leader for periplasmic secretion

Expression

Bacterial

Promoter

T7

Available Since

Aug. 17, 2021

Availability

Academic Institutions and Nonprofits only

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pFL-SV40-Rac1 5'-UTR

Plasmid

#115354

Purpose

firefly luciferase (Fluc) reporter containing the 5’-UTR of Rac1

Depositor

Ming-Chih Lai

Article

Ku et al Mol Cell Biol. 2018 Dec 11;39(1). pii: M

Insert

Rac1 5'-UTR (RAC1 Human)

Use

Luciferase

Expression

Mammalian

Available Since

Sept. 13, 2018

Availability

Academic Institutions and Nonprofits only

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pRC/CMV-TYK2V362F-VSV

Plasmid

#139345

Purpose

Expression of TYK2 V362F protein (rs2304256)

Depositor

Sandra Pellegrini

Article

Gauzzi et al J Biol Chem. 1996 Aug 23;271(34):20494-5

Insert

TYK2 cDNA with 267nt of 5' UTR (TYK2 Human)

Tags

VSV

Expression

Mammalian

Mutation

V362F

Promoter

CMV

Available Since

March 24, 2020

Availability

Academic Institutions and Nonprofits only

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pJL1-TTlight-4xDQNAT

Plasmid

#128394

Purpose

In vitro expression of tetanus toxin light chain with C terminal 4xDQNAT glycosylation sites

Depositor

Michael Jewett

Article

Stark et al Sci Adv. 2021 Feb 3;7(6). pii: 7/6/eabe9

Insert

Tetanus toxin light chain domain

Tags

4xDQNAT glycosylation tag and 6xHis tag

Expression

Bacterial

Mutation

Inactivating E234A mutation

Promoter

T7

Available Since

April 13, 2021

Availability

Academic Institutions and Nonprofits only

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pFL-SV40-GNB2 5'-UTR

Plasmid

#115355

Purpose

firefly luciferase (Fluc) reporter containing the 5’-UTR of GNB2

Depositor

Ming-Chih Lai

Article

Ku et al Mol Cell Biol. 2018 Dec 11;39(1). pii: M

Insert

GNB2 5'-UTR (GNB2 Human)

Use

Luciferase

Expression

Mammalian

Available Since

Sept. 28, 2018

Availability

Academic Institutions and Nonprofits only

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pET21-pelB-LaG-2

Plasmid

#172739

Purpose

Bacterial expression of HIS-tagged anti-GFP nanobody LaG-2; HIS tag preceded by HRV 3C/PreScission cleavage site.

Depositor

Michael Rout

Article

Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

Insert

LaG-2

Tags

6xHIS and pelB leader for periplasmic secretion

Expression

Bacterial

Promoter

T7

Available Since

Aug. 18, 2021

Availability

Academic Institutions and Nonprofits only

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