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pFUSEss-CHIg-mG3_F(ab')2

Plasmid

#105860

Purpose

Expression plasmid coding for heavy chain of mouse F(ab')2 of IgG3 antibody specific to antigen B of the ABO blood group system, clone M18.

Depositor

Joanna Bereta

Article

Klaus et al Front Immunol. 2018 May 23;9:1096. doi:

Insert

IgG3 F(ab')2 (Ighg3 Mouse)

Expression

Mammalian

Promoter

hEF1-HTLV prom

Available Since

March 20, 2018

Availability

Academic Institutions and Nonprofits only

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pDEST47-ARF4

Plasmid

#67455

Purpose

Mammalian expression of hArf4 with stop codon (native expression)

Depositor

Richard Kahn

Article

Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

Insert

ARF4 (ARF4 Human)

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only
pDEST17-6xHis-ARL2

Plasmid

#67474

Purpose

Expression of hArl2 with N-term His6 tag in E. coli

Depositor

Richard Kahn

Article

Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

Insert

ARL2 (ARL2 Human)

Tags

6xHIS

Expression

Bacterial

Promoter

T7

Available Since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only
pBlastR-5xUAS-mCherry-MiniWhite

Plasmid

#165911

Purpose

Blasticidin resistant 5xUAS mCherry Mini-White CDS response vector. Contains an Alpha level GB2.0 entry point for addition of custom assemblies utilizing blue-white bacterial colony screening

Depositor

Koen Venken

Article

Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

Insert

Selectable UAS Response Vector

Use

Synthetic Biology

Available Since

Sept. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pPuroR-MCS-GAL4-MiniWhite

Plasmid

#165890

Purpose

Puromycin resistant GAL4 driver vector with Mini-w+ CDS eye marker. Enhancer grammar GB20 entry point for custom enhancers. Cut-and-paste cloning can be used. Purple-white bacteria colony screening.

Depositor

Koen Venken

Article

Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

Insert

Selectable Empty Enhancer Driver

Use

Synthetic Biology

Available Since

Sept. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pDONR221-ARL8A

Plasmid

#67332

Purpose

hArl8a with stop codon in Gateway Entry vector

Depositor

Richard Kahn

Article

Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

Insert

ARL8A (ARL8A Human)

Use

Gateway entry vector

Expression

Bacterial

Promoter

None

Available Since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only
pBlastR-12xLexAOp-EBFP2-MiniWhite

Plasmid

#165918

Purpose

Blasticidin resistant 12xLexAOp EBFP2 Mini-White CDS response vector. Contains an Alpha level GB2.0 entry point for addition of custom assemblies utilizing blue-white bacterial colony screening

Depositor

Koen Venken

Article

Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

Insert

Selectable LexAOp Response Vector

Use

Synthetic Biology

Available Since

April 26, 2021

Availability

Academic Institutions and Nonprofits only

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pDONR221-ARL3

Plasmid

#67320

Purpose

hArl3 with stop codon in Gateway Entry vector

Depositor

Richard Kahn

Article

Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

Insert

ARL3 (ARL3 Human)

Use

Gateway entry vector

Expression

Bacterial

Promoter

None

Available Since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only
Ntn5.z-Fc-His

Plasmid

#72109

Purpose

Expresses the entire Netrin 5, isoform z protein, C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Ntn5 (Ntn5 Mouse)

Tags

Fc-His

Expression

Mammalian

Promoter

CMV

Available Since

March 11, 2016

Availability

Academic Institutions and Nonprofits only

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pWJPrP1

Plasmid

#22109

Depositor

Susan Lindquist

Article

Jackson et al Neuron. 2009 Aug 27. 63(4):438-50.

Insert

mouse PrP ORF insert (Prnp Mouse)

Use

Construction of targeting vector

Mutation

mutations at aa 108 and 111 to make 3F4 epitope (…

Available Since

Oct. 16, 2009

Availability

Academic Institutions and Nonprofits only
CMYA5T2/pDEST15

Plasmid

#98109

Purpose

Express CMYA5 (aa 4039 – 4069) Leu allele (4063) with a N-terminal GST tag in bacteria

Depositor

Rita Shiang

Article

Hsiung et al J Psychiatr Res. 2019 Apr;111:8-15. doi:

Insert

CMYA5 (CMYA5 Human)

Tags

GST

Expression

Bacterial

Mutation

rs10043986 (NP_705838.3:p.Pro4063Leu)

Available Since

Aug. 21, 2017

Availability

Industry, Academic Institutions, and Nonprofits

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LentiV_Neo_SIK3_T142Q

Plasmid

#108110

Purpose

Lentiviral expression plasmid of human SIK3 cDNA (gatekeeper mutation) with neomycin resistance gene

Depositor

Christopher Vakoc

Article

Tarumoto et al Mol Cell. 2018 Feb 28. pii: S1097-2765(1

Insert

SIK3 (SIK3 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Mutation

change Threonine 142 to Glutamine

Promoter

EFS promoter

Available Since

April 11, 2018

Availability

Academic Institutions and Nonprofits only

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pDONR221-ARF4

Plasmid

#67310

Purpose

hArf4 with stop codon in Gateway Entry vector

Depositor

Richard Kahn

Article

Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

Insert

ARF4 (ARF4 Human)

Use

Gateway entry vector

Expression

Bacterial

Promoter

None

Available Since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only
pET-DEST42-ARL3

Plasmid

#67418

Purpose

Expression of hArl3 in E. coli

Depositor

Richard Kahn

Article

Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

Insert

ARL3 (ARL3 Human)

Expression

Bacterial

Promoter

T7/lacO

Available Since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only
pDSHA-ARL4C-Myc

Plasmid

#67360

Purpose

Mammalian expression of hArl4c with C-term Myc tag

Depositor

Richard Kahn

Article

Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

Insert

ARL4C (ARL4C Human)

Tags

Myc

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only
CS2-Myc-mASUN

Plasmid

#47044

Purpose

Expression of tagged-mouse ASUN

Depositor

Laura Lee

Article

Jodoin et al Mol Biol Cell. 2012 Dec;23(24):4713-24.

Insert

Asunder (Asun Mouse)

Tags

Myc

Expression

Mammalian

Available Since

Sept. 10, 2013

Availability

Academic Institutions and Nonprofits only
pDSHA-ARL4-Myc

Plasmid

#67356

Purpose

Mammalian expression of hArl4 with C-term Myc tag

Depositor

Richard Kahn

Article

Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

Insert

ARL4 (ARL4A Human)

Tags

Myc

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only
pDONR221-ARL4

Plasmid

#67322

Purpose

hArl4 with stop codon in Gateway Entry vector

Depositor

Richard Kahn

Article

Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

Insert

ARL4 (ARL4A Human)

Use

Gateway entry vector

Expression

Bacterial

Mutation

bp 105 A to T; silent mutation

Promoter

None

Available Since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only
pDONR221-ARL4C

Plasmid

#67330

Purpose

hArl4c with stop codon in Gateway Entry vector

Depositor

Richard Kahn

Article

Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

Insert

ARL7 (ARL4C Human)

Use

Gateway entry vector

Expression

Bacterial

Promoter

None

Available Since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only
pDONR221-ARL2

Plasmid

#67318

Purpose

hArl2 with stop codon in Gateway Entry vector

Depositor

Richard Kahn

Article

Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

Insert

ARL2 (ARL2 Human)

Use

Gateway entry vector

Expression

Bacterial

Promoter

None

Available Since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only