We narrowed to 7,572 results for: Lif

1. AAVS1-SA-neo-CAGGS-PE2-2A-GFP

   Plasmid

   #180014

   Purpose

   Targeting vector for knocking in constitutively expressed PE2-2A-GFP into the human AAVS1 locus

   Depositor

   Dirk Hockemeyer

   Article

   Li et al Elife. 2022 Sep 7;11:e79208. doi: 10.755

   Insert

   PE2-2A-GFP
   

   Use

   CRISPR; Human genome engineering

   Tags

   GFP

   Expression

   Mutation

   Promoter

   CAG

   Available since

   Sept. 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAV-DIO-Ef1a-TVA-2A-oG

   Plasmid

   #172359

   Purpose

   Targeting envA-pseodotyped RVdG-CVS-N2c vectors for specific retrograde labeling specifically from cre-expressing neurons

   Depositor

   Yoav Ben-Simon, Peter Jonas

   Article

   Sumser et al Elife. 2022 Aug 30;11:e79848. doi: 10.75

   Insert

   TVA + B19-oG
   

   Use

   AAV and Cre/Lox

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Sept. 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. mCerulean3-BclXL-pEGFP-C1

   Plasmid

   #177408

   Purpose

   To express mCerulean3 fused to the N-terminus of the Bcl-2 family protein, Bcl-XL.

   Depositor

   David Andrews

   Article

   Liu et al Elife. 2019 Mar 12;8. pii: 37689. doi: 1

   Insert

   Bcl-XL
   

   Use

   Tags

   mCerulean3

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pGEMHE-mCherry-mTrim21ΔPRYSPRY

   Plasmid

   #105524

   Purpose

   T7 promotor drives in vitro transcription of mCherry-tagged mouse TRIM21 with deleted PRYSPRY domain mRNA

   Depositor

   Melina Schuh

   Article

   Clift et al Cell. 2017 Nov 13. pii: S0092-8674(17)31

   Insert

   TRIM21 (Trim21 Mouse)
   

   Use

   In vitro transcription

   Tags

   mCherry

   Expression

   Mutation

   deletion of PRYSPRY domain (aa1-281)

   Promoter

   T7

   Available since

   March 7, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV-FRT-EF1a-TVA-2A-N2cG

   Plasmid

   #172361

   Purpose

   Targeting envA-pseodotyped RVdG-CVS-N2c vectors for specific retrograde labeling from Flp-expressing neurons

   Depositor

   Yoav Ben-Simon, Peter Jonas

   Article

   Sumser et al Elife. 2022 Aug 30;11:e79848. doi: 10.75

   Insert

   TVA + B19-N2cG
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Dec. 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pFE-Selo-FI-prk

   Plasmid

   #162698

   Purpose

   Expresses S. elongatus Form IB rubisco and S. elongatus Prk

   Depositor

   David Savage

   Article

   Flamholz et al Elife. 2020 Oct 21;9. pii: 59882. doi: 1

   Insert

   S. elongatus Form IB rubisco and S. elongatus Prk
   

   Use

   Tags

   N terminal 6x His tag on PRK

   Expression

   Bacterial

   Mutation

   Promoter

   PLtet0-1 promoter

   Available since

   Feb. 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCS2+ mWnt5a

   Plasmid

   #102860

   Purpose

   Expresses mouse Wnt5a in mammalian cells, zebrafish or Xenopus

   Depositor

   Henry Ho

   Article

   Susman et al Elife. 2017 Sep 8;6. pii: e26509. doi: 1

   Insert

   Mouse Wnt5a
   

   Use

   Zebrafish expression; xenopus expression

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV; SP6

   Available since

   Dec. 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCA528 AMSH 1-178

   Plasmid

   #160050

   Purpose

   Expresses MIT domain (residues 1-178) of AMSH as a HIS-SUMO fusion protein. Internal ID: WISP18-22.

   Depositor

   Wesley Sundquist

   Article

   Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

   Insert

   AMSH residues 1-178 (STAMBP Human)
   

   Use

   Tags

   HIS-SUMO

   Expression

   Bacterial

   Mutation

   Residues 1-178

   Promoter

   T7

   Available since

   July 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. PP023 - CAG::QuasAr6a-TS-dmCitrine-TSx3-ER2-p2a- jRGECO1a-CAAX

   Plasmid

   #182063

   Purpose

   Co-expression of Arch-based voltage indicator (QuasAr6a) and membrane-localized calcium reporter (jRGECO1a-CAAX)

   Depositor

   Adam Cohen

   Article

   Landau et al eLife. 2022 Mar 23;11. pii: 76993. doi:

   Insert

   QuasAr6a-TS-dmCitrine-TSx3-ER2-p2a- jRGECO1a-CAAX
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   CAG

   Available since

   March 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pSMT3-FBF2

    Plasmid

    #166972

    Purpose

    Expresses FBF-2 in E. coli for purification and crystallization studies

    Depositor

    Traci Hall

    Article

    Bhat et al Elife. 2019 Jan 17;8. pii: 43788. doi: 1

    Insert

    FBF-2 (fbf-2 Nematode)
    

    Use

    Tags

    His-SUMO

    Expression

    Bacterial

    Mutation

    FBF-2 PUM RNA binding domain; amino acids 164-575

    Promoter

    T7

    Available since

    April 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. Fireworks GFP[PTC-] (AVA2598)

    Plasmid

    #85445

    Purpose

    Expresses TEV protease and tandemly-repeated GFP fluorescent proteins to amplify the fluorescence signal produced by a single transcription unit of a beta-globin reporter.

    Depositor

    Joan Steitz

    Article

    Alexandrov et al Mol Cell. 2017 Jan 5;65(1):191-201. doi:

    Insert

    tDNA1 EF1alfa-5xEGFP-TEVprotease-PEST-beta-globin(dI1)-BGH polyA tDNA2 FRT-PEST-TEVsite-PuromycinR
    

    Use

    Minimal backbone fragment for low-copy bacterial …

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. mCherry-p62-LIR-mutant (LIRAAAA)

    Plasmid

    #187981

    Purpose

    Plasmid for the expression of a p62 mutant (LIR mutant: p62 3xDW + silent mutation). Internal reference: SMC528

    Depositor

    Sascha Martens

    Article

    Wurzer et al Elife. 2015 Sep 28;4:e08941. doi: 10.755

    Insert

    p62 (NUP62 Human)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    DDDW335-338AAAA (LIR mutant), silent mutation siR…

    Promoter

    Available since

    Oct. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. Sema3c(L)-AP-His

    Plasmid

    #72015

    Purpose

    Expresses the Sema3C protein (truncated at cleavage site P3; ie, long), C-terminally fused to alkaline phosphatase + 6X histidine tag.

    Depositor

    Woj Wojtowicz

    Article

    Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

    Insert

    Sema3c (Sema3c Mouse)
    

    Use

    Tags

    AP-His

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 24, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. Fireworks RFP[PTC-] (AVA2515)

    Plasmid

    #85443

    Purpose

    Expresses TEV protease and tandemly-repeated RFP fluorescent proteins to amplify the fluorescence signal produced by a single transcription unit of a beta-globin reporter.

    Depositor

    Joan Steitz

    Article

    Alexandrov et al Mol Cell. 2017 Jan 5;65(1):191-201. doi:

    Insert

    tDNA1 EF1alfa-5xtdTomato-TEVprotease-PEST-beta-globin(dI1)-BGH polyA tDNA2 FRT-HygromycinR
    

    Use

    Minimal backbone fragment for low-copy bacterial …

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pENTR_L1-Turbo-NES-YFP-STOP-L2

    Plasmid

    #127358

    Purpose

    gateway entry vector for expressing cytosolic TurboID-YFP under a promoter of choice

    Depositor

    Dominique Bergmann

    Article

    Mair et al Elife. 2019 Sep 19;8. pii: 47864. doi: 1

    Insert

    TurboID (BirA mutant)
    

    Use

    Gateway entry vector

    Tags

    GS linker, NES, V5, and YFP

    Expression

    Mutation

    Q65P, I87V, R118S, E140K, Q141R, S150G, L151P, V1…

    Promoter

    no promoter

    Available since

    Sept. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA3.1-TIE-V5/HIS

    Plasmid

    #205612

    Purpose

    Expression of human TIE receptor tyrosine kinase in mammalian cells

    Depositor

    Pavel Krejčí

    Article

    Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:

    Insert

    TIE receptor tyrosine kinase (TIE1 Human)
    

    Use

    Tags

    V5-His

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pFE-Rrub-FII-prk

    Plasmid

    #162695

    Purpose

    Expresses R. rubrum Form II rubisco and S. elongatus Prk

    Depositor

    David Savage

    Article

    Flamholz et al Elife. 2020 Oct 21;9. pii: 59882. doi: 1

    Inserts

    Rubrum Form II rubisco
    S. elongatus PRK
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PLtet0-1 promoter and under same promoter

    Available since

    Feb. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. CVS-N2c-nl.mCherry-GCaMP7s

    Plasmid

    #172385

    Purpose

    Production of RVdG-CVS-N2c viral vectors for cell-type specific trans-synaptic retrograde labeling.

    Depositor

    Yoav Ben-Simon, Peter Jonas

    Article

    Sumser et al Elife. 2022 Aug 30;11:e79848. doi: 10.75

    Insert

    nuclear-localized mCherry + GCaMP7s
    

    Use

    Neurotropic virus

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLX317-hcRED

    Plasmid

    #115440

    Purpose

    Constitutive lentiviral expression

    Depositor

    William Hahn

    Article

    Li et al Elife. 2018 Jul 30;7. pii: 37184. doi: 1

    Insert

    hcRED
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Dec. 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pHAGE-FLAG-APEX2-CALCOCO2

    Plasmid

    #175759

    Purpose

    Lentiviral vector expressing N-terminally tagged APEX2-FLAG-CALCOCO2/NDP52

    Depositor

    Wade Harper

    Article

    Eapen et al eLife. 2021 Sep 29;10. pii: 72328. doi:

    Insert

    NDP52/CALCOCO2 (CALCOCO2 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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