We narrowed to 2,463 results for: T2A

1. pPN459

   Plasmid

   #137875

   Purpose

   PiggyBac vector for expression of 1x gRNA targeting TCF4 for CRISPRi; mRFP-T2A-BlasticidinR

   Depositor

   Lindy Barrett

   Article

   Hazelbaker et al Sci Rep. 2020 Jan 20;10(1):635. doi: 10.

   Insert

   TCF4 (TCF4 Human)
   

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   Feb. 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. MOBE3

   Plasmid

   #219943

   Purpose

   Mammalian expression of MOBE3 protein (apt-CBE-SL3_PT2A_apt-ABE8e)

   Depositor

   Alexis Komor

   Article

   Cowan et al Nat Biotechnol. 2024 May 21. doi: 10.103

   Insert

   apt-CBE-SL3_PT2A_apt-ABE8e
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 3, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. MOBE4

   Plasmid

   #219944

   Purpose

   Mammalian expression of MOBE4 protein (apt-CBE-SL3_PT2A_apt-ABE8.20)

   Depositor

   Alexis Komor

   Article

   Cowan et al Nat Biotechnol. 2024 May 21. doi: 10.103

   Insert

   apt-CBE-SL3_PT2A_apt-ABE8.20
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 30, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. MOBE2

   Plasmid

   #219942

   Purpose

   Mammalian expression of MOBE2 protein (apt-CBE-3'end_PT2A_apt-ABE8.20)

   Depositor

   Alexis Komor

   Article

   Cowan et al Nat Biotechnol. 2024 May 21. doi: 10.103

   Insert

   apt-CBE-3'end_PT2A_apt-ABE8.20
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 3, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pSN993

   Plasmid

   #219961

   Purpose

   Expresses CMT2A mutant version of human KIF1Bß in C. elegans

   Depositor

   Shinsuke Niwa

   Insert

   unc-104 promoter::human KIF1Bß(Q98L) (KIF1B Human, Nematode)
   

   Expression

   Worm

   Available Since

   July 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pACEBac1-ZZ-TEV-MZT2-3C-mEGFP

   Plasmid

   #178074

   Purpose

   Insect cell expression of ZZ-TEV-MZT2-3C-mEGFP

   Depositor

   Tarun Kapoor

   Article

   Wieczorek et al J Cell Biol. 2021 Mar 1;220(3). pii: 211

   Insert

   MZT2A (MZT2A Human)
   

   Tags

   PreScission-mEGFP and ZZ-TEV

   Expression

   Insect

   Available Since

   Feb. 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCDH-EF1a-eFFly-mCherry

   Plasmid

   #104833

   Purpose

   3rd generation lentiviral vector. eFFly luciferase for in vivo Imaging, mCherry for fluorescent detection

   Depositor

   Irmela Jeremias

   Article

   Ebinger et al Cancer Cell. 2016 Dec 12;30(6):849-862.

   Inserts

   eFFly
   mCherry
   

   Use

   Lentiviral and Luciferase

   Expression

   Mammalian

   Available Since

   Aug. 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAVS1-Neo-CAG-M2rtTA-H2BmNeon (donorAB-mNeon)

   Plasmid

   #85799

   Purpose

   donorAB-Neon targeting vector for human AAVS1 locus; knock-in of Dox controlled H2BGFP

   Depositor

   Roland Friedel

   Article

   Tejero et al EBioMedicine. 2019 Apr 2. pii: S2352-396

   Insert

   PPP1R12C (PPP1R12C Human)
   

   Use

   Human targeting

   Mutation

   homology arms for knock-in into first intron

   Available Since

   April 26, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pTet-O-Ngn2-puro

   Plasmid

   #52047

   Purpose

   3rd generation lentiviral vector, expresses Ngn2 and puromycin resistent gene under control of TetON promoter

   Depositor

   Marius Wernig

   Article

   Zhang et al Neuron. 2013 Jun 5;78(5):785-98. doi: 10

   Insert

   Ngn2 (Neurog2 Mouse)
   

   Use

   Lentiviral

   Tags

   T2A-and puromycin resistant cassette

   Expression

   Mammalian

   Promoter

   tet-on

   Available Since

   April 11, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCDH-EF1a-mCherry-EGFP-LC3B

    Plasmid

    #170446

    Purpose

    Lentiviral vector expressing tandem mCherry-EGFP-LC3B in mamalian cell. To visualize free autophagosome and autophagosomes that have fused with the lysosome.

    Depositor

    Sang-Hun Lee

    Article

    Wulansari et al Sci Adv. 2021 Feb 17;7(8). pii: 7/8/eabb

    Insert

    mCherry-GFP-LC3 (MAP1LC3B Human)
    

    Use

    Lentiviral

    Promoter

    EF1a

    Available Since

    June 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pgRNA-CKB

    Plasmid

    #73501

    Purpose

    gRNA expression vector (with mKate2)

    Depositor

    Bruce Conklin

    Article

    Mandegar et al Cell Stem Cell. 2016 Apr 7;18(4):541-53.

    Insert

    mKate2-T2A-Bsd
    

    Use

    CRISPR and Lentiviral

    Tags

    NLS

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Dec. 7, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. PE7_AAVS1_knockin_HDR_donor

    Plasmid

    #223001

    Purpose

    HDR donor plasmid for PE7 knockin at AAVS1 locus. Left_homology_arm-splicing_acceptor-T2A-NeoR-bGHpA-pEF1α-PE7-IRES2-EGFP-WPRE-βglobinpA-right_homology_arm

    Depositor

    Brittany Adamson

    Article

    Yan et al Nature. 2024 Apr 3. doi: 10.1038/s41586-

    Insert

    PE7
    

    Use

    Hdr donor

    Tags

    SV40 bpNLS and c-Myc NLS

    Expression

    Mammalian

    Promoter

    EF1α

    Available Since

    Aug. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLenti_P2A_Puro

    Plasmid

    #211364

    Purpose

    Lentiviral expression vector for an insert of interest linked to a P2A-T2A puromycin sequence. Produces virus very efficiently. Can also be used for regular transfections

    Depositor

    Jacques Neefjes

    Article

    Middelburg et al Cell Rep. 2023 Dec 26;42(12):113516. doi

    Type

    Empty backbone

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    Jan. 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAAVS1-Neo-CAG-M2rtTA-H2BGFP (donor AB)

    Plasmid

    #85798

    Purpose

    donorAB targeting vector for human AAVS1 locus; knock-in of Dox controlled H2BGFP expression system

    Depositor

    Roland Friedel

    Article

    Tejero et al EBioMedicine. 2019 Apr 2. pii: S2352-396

    Insert

    PPP1R12C (PPP1R12C Human)
    

    Use

    Human targeting

    Mutation

    homology arms for knock-in into first intron

    Available Since

    March 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. AAVS1 CAG rtTA3 TauWT 2N4R-EGFP

    Plasmid

    #132389

    Purpose

    Exchange cassette containing inducible TauWT 2N4R-EGFP fusion, CAG-driven rtTA3, Puromycin resistance marker, targets AAVS1 site

    Depositor

    Gerold Schmitt-Ulms

    Article

    Wang et al Bio Protoc. 2020 May 5;10(9):e3615. doi:

    Insert

    human TauWT 2N4R-EGFP (MAPT Human, Synthetic)
    

    Use

    CRISPR and Cre/Lox

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    TREtight

    Available Since

    May 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLenti-H3.3(K36M)-3AID-HA-2A-mCherryBSD

    Plasmid

    #225699

    Purpose

    Lentiviral expression of AID degron tagged H3.3(K36M) and mCherry fused BlasticidinR

    Depositor

    Lacra Bintu

    Article

    Sinha et al bioRxiv [Preprint]. 2023 Oct 14:2023.10.

    Insert

    H3.3 (K36M) (H3-3A, H3-3B Human)
    

    Use

    Lentiviral and Synthetic Biology

    Tags

    3xAID HA and T2A-mCherry

    Expression

    Mammalian

    Mutation

    K36M

    Promoter

    pEF1A

    Available Since

    Oct. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. FUW-SOK

    Plasmid

    #20327

    Depositor

    Rudolf Jaenisch

    Article

    Carey et al Proc Natl Acad Sci U S A. 2009 Jan 6. 10

    Insert

    Sox2-F2A-Oct4-T2A-Klf4 (Sox2, Pou5f1, Klf4 Mouse)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    Feb. 23, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLenti-H3.3(K27M)-3AID-HA-2A-mTurquoiseBSD

    Plasmid

    #225702

    Purpose

    Lentiviral expression of AID degron tagged H3.3(K27M) and mTurquoise fused BlasticidinR

    Depositor

    Lacra Bintu

    Article

    Sinha et al bioRxiv [Preprint]. 2023 Oct 14:2023.10.

    Insert

    H3.3 (K27M) (H3-3A, H3-3B Human)
    

    Use

    Lentiviral and Synthetic Biology

    Tags

    3xAID HA and T2A-mTurquoise

    Expression

    Mammalian

    Mutation

    K27M

    Promoter

    pEF1A

    Available Since

    Oct. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pMXs-LNI (with mNgn2x3HA)

    Plasmid

    #233195

    Purpose

    Retroviral expression of Lhx3, Ngn2x3HA, and Isl1 for motor neuron reprogramming

    Depositor

    Kate Galloway

    Article

    Wang et al Cell Syst. 2025 Mar 10:101206. doi: 10.1

    Insert

    mLhx3-P2A-mNgn2x3HA-T2A-Isl1 (Neurog2, Isl1, Lhx3 Mouse)
    

    Use

    Retroviral

    Tags

    x3HA

    Expression

    Mammalian

    Available Since

    March 13, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCDH-EF1a-extGluc-mKate-NGFR

    Plasmid

    #104831

    Purpose

    extGluc luciferase for invivo Imaging, mKate and NGFR for flourescent detection

    Depositor

    Irmela Jeremias

    Article

    Ebinger et al Cancer Cell. 2016 Dec 12;30(6):849-862.

    Inserts

    mKate
    NGFR
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    Oct. 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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