We narrowed to 15,981 results for: nes
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Plasmid#84379PurposeExpression of dcas9 D10A H840A from a TetR-regulated uvtetO promoterDepositorInsertdcas9
UseCRISPRExpressionBacterialMutationAspartate at position 10 changed to Alanine, Hist…Promoteruv15tetOAvailable SinceFeb. 1, 2017AvailabilityAcademic Institutions and Nonprofits only -
pGolt-mCherry / pGolt3-mCherry
Plasmid#73297PurposeVisualization of Dendritic Golgi SatelliteDepositorInsertsER export signal of Scap
ER export signal of Scap
TMD (trans-membrane domain) of Calneuron-2 (Cabp7 Rat)
TagsER export and mCherryExpressionMammalianPromoterCMV immediate early promoterAvailable SinceFeb. 18, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJSC114 - Bacterial expression plasmid for SpCas9 eSpCas9(1.1) variant
Plasmid#101215PurposeBacterial expression plasmid for SpCas9 eSpCas9(1.1) variantDepositorInsertSpCas9 variant K848A/K1003A/R1060A
Tags10x His, MBP, and TEV siteExpressionBacterialMutationK848A, K1003A and R1060APromoterT7Available SinceOct. 4, 2017AvailabilityAcademic Institutions and Nonprofits only -
pEcCas-2.0
Plasmid#190697PurposeConstitutive expressing of Cas9 and inducible expression of lambda RedDepositorInsertCas9 and Lamda Red system
ExpressionBacterialAvailable SinceMarch 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDNR-SpCas9-Gem
Plasmid#80424PurposeUsed for in vitro transcription of SpCas9-Gem mRNA. Cas9 is fused to Geminin peptide and is degraded during G0/G1 phases of the cell-cycle to minimize indels caused by non-homologous end joiningDepositorInsertSpCas9-Gem
UseCRISPRTagsGemininMutationcodon optimized for humanPromoterT7Available SinceAug. 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
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LLP457 pGK-dCas9-Suntag-BFP
Plasmid#100957PurposeBPF version of LLP339, Puro is replaced by BFP driven by EF1a, dCas9 with SungTagx10 driven by pGK, with 3xHA tagDepositorInsertdCas9, SunTag array (10xGCN4)
UseCRISPRTags3xHA and BFPExpressionMammalianMutationD10A, H840A for dCas9Available SinceJune 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRB14
Plasmid#52522Purposeexpresses a myc-tagged version of hCas9 in DrosophilaDepositorInsertS. pyogenes cas9 with humanized codon bias
TagsmycExpressionInsectPromotertubulinAvailable SinceApril 22, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAc-mCh-Tub
Plasmid#24288DepositorAvailable SinceApril 23, 2010AvailabilityAcademic Institutions and Nonprofits only -
pCTX-CymRGV-MCS
Plasmid#253932PurposeCTX based integration vector containing PCymRC and the codon optimised CymRGV. Derived from pAH-CTX-Rha replacing the rhamnose induction circuit with PCymRC/CymRGV upstream of a MCS, tetR.DepositorInsertCymR inducible systems
ExpressionBacterialAvailable SinceApril 8, 2026AvailabilityAcademic Institutions and Nonprofits only -
pCTX-CymRGV-sfGFP
Plasmid#253933PurposeCTX based integration vector containing PCymRC and the codon optimised CymRGV. Derived from pAH-CTX-Rha replacing the rhamnose induction circuit with PCymRC/CymRGV controlling sfGFP, tetR.DepositorInsertCymR inducible systems
ExpressionBacterialAvailable SinceApril 8, 2026AvailabilityAcademic Institutions and Nonprofits only -
pTE_10 - pCMV-Cas9_only
Plasmid#252006PurposeMammalian expression of Cas9DepositorInsertCas9
UseCRISPRExpressionMammalianMutationNAAvailable SinceMarch 4, 2026AvailabilityAcademic Institutions and Nonprofits only -
pTE_23 - pDonor_TRAC-2a-FMC6.3-BBz-2a
Plasmid#252019PurposeDonor for CD19 CAR knock-in at TRAC locusDepositorInsert2a-FMC6.3-BBz-2a
UseUnspecifiedMutationNAAvailable SinceMarch 4, 2026AvailabilityAcademic Institutions and Nonprofits only -
pEM03
Plasmid#135417PurposeExpression of Hygromycin resistance from Spizellomyces Hsp70 promoterDepositorInsertSpunHsp70pr
ExpressionBacterial and YeastPromoterHsp70prAvailable SinceMay 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGI3EM29
Plasmid#135489PurposeAgrobacterium T-DNA with Spun H2Bpr driving hph-mCitrineDepositorInserthph SpunH2A/Bpr hph-mCitrine
TagsmCitrineExpressionBacterial and YeastPromoterSpunH2A/Bpr (divergent)Available SinceMay 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGI3EM30
Plasmid#135490PurposeAgrobacterium T-DNA with Spun H2Bpr driving hph-mClover3DepositorInserthph SpunH2A/Bpr hph-mClover3
TagsmClover3ExpressionBacterial and YeastPromoterSpunH2A/Bpr (divergent)Available SinceMay 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
pNB786
Plasmid#60151PurposeFirefly luciferase (Promega) yeast codon-optimized yellow fluorescent protein (yEVenus) fusion driven by LEU1 promoter.DepositorInsertFLuc-yEVenus
ExpressionYeastMutationFLuc has A4V & S504G (no functional effect)PromoterLEU1prAvailable SinceMarch 25, 2015AvailabilityAcademic Institutions and Nonprofits only -
pNB682
Plasmid#60143PurposeFirefly luciferase (Promega) and yeast codon-optimized yellow fluorescent protein (yEVenus) fusion with PEST degron driven by MET17 promoter.DepositorInsertFLuc-yEVenus-PEST
TagsPEST degronExpressionYeastMutationA4VPromoterMET17prAvailable SinceMarch 25, 2015AvailabilityAcademic Institutions and Nonprofits only -
pNB693
Plasmid#60145PurposeDisintegrator plasmid encoding MET17 promoter driven yellow beetle luciferase with nuclear localization signal (NLS) and ClpXP degron tag (ssrA).DepositorInsertYeLuc
TagsClpXP degron and Nuclear localization signal (NLS…ExpressionYeastPromoterMET17prAvailable SinceDec. 22, 2014AvailabilityAcademic Institutions and Nonprofits only -
pNB689
Plasmid#60133PurposeGreen beetle luciferase with nuclear localization signal (NLS) and ClpXP degron tag (ssrA), driven by MET17 promoter.DepositorInsertGrLuc
TagsClpXP degron and Nuclear localization signal (NLS…ExpressionYeastPromoterMET17prAvailable SinceOct. 31, 2014AvailabilityAcademic Institutions and Nonprofits only