We narrowed to 12,157 results for: pcdna

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pcDNA5/FRT/TO_HaloTag7_LamB1

Plasmid

#175528

Purpose

Expression of HaloTag7 at the nuclear lamina of the mammalian cells

Depositor

Kai Johnsson

Article

Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

Insert

HaloTag7-LamB1

Expression

Mammalian

Available Since

Dec. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO HSPA8

Plasmid

#19460

Depositor

Harm Kampinga

Article

Hageman et al Cell Stress Chaperones. 2009 Jan;14(1):1

Insert

HSPA8 (HSPA8 Human)

Expression

Mammalian

Available Since

Oct. 6, 2008

Availability

Academic Institutions and Nonprofits only

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pcDNA3-ALK6 Q203D

Plasmid

#80883

Purpose

mammalian expression of ALK6 Q203D

Depositor

Aristidis Moustakas

Article

Raja et al Oncotarget. 2016 Jan 12;7(2):1120-43. do

Insert

ALK6 (Bmpr1b Mouse)

Tags

HA

Expression

Mammalian

Mutation

Q203D (constitutively active)

Promoter

CMV

Available Since

Oct. 17, 2016

Availability

Academic Institutions and Nonprofits only
pCDNA_Lifeact-GFP_NLS-mCherry

Plasmid

#69058

Purpose

Dual Fluorescent Reporter for F-actin and Nuclei

Depositor

Olivier Pertz

Insert

LifeAct-GFP_IRES_NLS-mCherry

Tags

Lifeact-GFP, NLS-mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 23, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-TRKC-V5/HIS

Plasmid

#205615

Purpose

Expression of human TRKC receptor tyrosine kinase in mammalian cells

Depositor

Pavel Krejčí

Article

Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:

Insert

TRKC receptor tyrosine kinase (NTRK3 Human)

Tags

V5-His

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 11, 2023

Availability

Academic Institutions and Nonprofits only

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pCDNA3-AUP1-FLAG

Plasmid

#185336

Purpose

Mammalian expression of AUP1

Depositor

Allan Weissman

Article

Smith et al PLoS Biol. 2021 Dec 8;19(12):e3001474. d

Insert

AUP1 (AUP1 Human)

Tags

FLAG, 6xHis

Expression

Mammalian

Available Since

Jan. 17, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1/VChR1-EYFP

Plasmid

#20955

Purpose

Mammalian expression of VChR1 fused to EYFP, for neuronal excitation

Depositor

Karl Deisseroth

Article

Zhang et al Nat Neurosci. 2008 Jun . 11(6):631-3.

Insert

volvox channelrhodopsin-1

Tags

EYFP

Expression

Mammalian

Promoter

CMV

Available Since

May 13, 2009

Availability

Academic Institutions and Nonprofits only

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pcDNA3-RAB-ICUE

Plasmid

#118480

Purpose

ddRFP-based single-color biosensor for monitoring cAMP dynamics.

Depositor

Jin Zhang

Article

Mehta et al Nat Cell Biol. 2018 Sep 24. pii: 10.1038

Insert

RAB-ICUE

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 7, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-Bsd-mEGFP

Plasmid

#214369

Purpose

Expression of the monomeric Green Fluorescent Protein

Depositor

Bertrand Collet

Insert

mEGFP

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO V5 HSPH1

Plasmid

#19506

Depositor

Harm Kampinga

Article

Hageman et al Cell Stress Chaperones. 2009 Jan;14(1):1

Insert

heat shock 105kDa/110kDa protein 1 (HSPH1 Human)

Tags

V5

Expression

Mammalian

Available Since

Oct. 6, 2008

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)-FluoSTEP-ICUE

Plasmid

#181845

Purpose

Genetically encoded FRET-based sensor for monitoring cAMP dynamics near a protein of interest. Must pair with GFP11-tagged POI to reconstitute donor fluorescence.

Depositor

Jin Zhang

Article

Zhang et al Cell. 2020 Sep 17;182(6):1531-1544.e15.

Insert

FluoSTEP-ICUE (RAPGEF3 Human)

Tags

mRuby2 and sfGFP(1-10)

Expression

Mammalian

Promoter

CMV

Available Since

July 6, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA4-STRAD alpha

Plasmid

#14889

Depositor

Lewis Cantley

Article

Shaw et al Proc Natl Acad Sci U S A 2004 Mar 9;101(

Insert

STRAD alpha (STRADA Human)

Tags

6xHis/Xpress

Expression

Mammalian

Available Since

April 27, 2007

Availability

Academic Institutions and Nonprofits only
pcDNA4-AMBRA1-3xFLAG

Plasmid

#174157

Purpose

Vector for expression of 3xFLAG-tagged wild-type AMBRA1

Depositor

Julien Sage

Article

Chaikovsky et al Nature. 2021 Apr;592(7856):794-798. doi:

Insert

AMBRA1 (AMBRA1 Human)

Tags

3xFLAG

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)-ExRai-AKAR2(T/A)

Plasmid

#161754

Purpose

Negative-control mutant for ExRai-AKAR2 biosensor.

Depositor

Jin Zhang

Article

Zhang et al Nat Chem Biol. 2020 Sep 28. pii: 10.1038

Insert

ExRai-AKAR2(T/A)

Expression

Mammalian

Mutation

Contains Thr-to-Ala mutation in substrate sequenc…

Promoter

CMV

Available Since

Nov. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pCDNA3.1-Flag-H2A

Plasmid

#63560

Purpose

Expression of human H2A tagged with Flag

Depositor

Titia Sixma

Article

Mattiroli et al Cell. 2012 Sep 14;150(6):1182-95. doi: 1

Insert

H2A (H2AC13 Human)

Tags

Flag (MDYKDDDDKVPRIQ)

Expression

Mammalian

Promoter

CMV

Available Since

March 16, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA3 GFP-h-PKD2

Plasmid

#83451

Purpose

GFP-tagged PC2 for Eukaryotic expression (883)

Depositor

Thomas Weimbs

Article

Doerr et al PLoS One. 2016 Aug 25;11(8):e0161525. do

Insert

Polycystin-2 (PKD2 Human)

Tags

GFP

Expression

Mammalian

Available Since

Aug. 9, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Flag Rac2

Plasmid

#12192

Depositor

Gary Johnson

Article

Abell et al J Cell Sci. 2004 Jan 15. 117(Pt 2):243-5

Insert

Rac2 (RAC2 Human)

Tags

Flag

Expression

Mammalian

Available Since

Oct. 3, 2006

Availability

Academic Institutions and Nonprofits only

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pCDNA5/FRT/TO_H2B_HaloTag9_T2A_EGFP

Plasmid

#169332

Purpose

Nuclear expression of HaloTag9 in mammalian cells

Depositor

Kai Johnsson

Article

Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

Insert

H2B-HaloTag9 (H2BC21 Human)

Tags

HaloTag

Expression

Mammalian

Mutation

HaloTag9 = HaloTag7-Q165H-P174R

Available Since

Nov. 9, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3-PDE4D2cat-ICUE4

Plasmid

#181847

Purpose

ICUE4 cAMP sensor tethered to the catalytic domain of PDE4D2.

Depositor

Jin Zhang

Article

Zhang et al Cell. 2020 Sep 17;182(6):1531-1544.e15.

Insert

PDE4D2cat-ICUE4 (RAPGEF3, PDE4D Human)

Tags

ECFP, PDE4D2(86-418), and cpVenus(L194)

Expression

Mammalian

Mutation

Glutamine 122 in Epac1 domain mutated to glutamat…

Promoter

CMV

Available Since

June 17, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3.4_1G11F2-3 heavy chain

Plasmid

#198253

Purpose

recombinant expression of heavy chain of monoclonal anti-AMP antibody 1G11F2-3

Depositor

Aymelt Itzen

Article

Hopfner et al iScience. 2020 Nov 13;23(12):101800. doi

Insert

heavy chain of monoclonal anti-AMP antibody 1G11F2-3 (mouse IgG2b)

Tags

IL10 signal peptide

Expression

Mammalian

Promoter

CMV

Available Since

May 5, 2023

Availability

Academic Institutions and Nonprofits only

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