We narrowed to 11,992 results for: pcdna

1. pcDNA3.1-Cry2FL-VP64

   Plasmid

   #60554

   Purpose

   Expresses Cry2FL-VP64 in mammalian cells

   Depositor

   Charles Gersbach

   Article

   Polstein et al Nat Chem Biol. 2015 Feb 9. doi: 10.1038/

   Insert

   Cry2FL-VP64
   

   Use

   Tags

   FLAG, HA tag, and SV40 NLS

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Jan. 30, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3-sACE2(WT)-Fc(IgA1)

   Plasmid

   #145154

   Purpose

   Mammalian expression plasmid for soluble ACE2-Fc fusion (IgA1)

   Depositor

   Erik Procko

   Article

   Chan et al Science. 2020 Sep 4;369(6508):1261-1265.

   Insert

   Angiotensin-converting enzyme 2 (ACE2 Human)
   

   Use

   Tags

   Fc region of human IgA1

   Expression

   Mammalian

   Mutation

   Extracellular, soluble protease domain (a.a. M1-D…

   Promoter

   CMV

   Available Since

   April 15, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3-Casp2-Flag

   Plasmid

   #11811

   Depositor

   Guy Salvesen

   Insert

   Caspase-2 (CASP2 Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   May 12, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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4. Dead-Laconic/pcDNA3.1(-)

   Plasmid

   #118627

   Purpose

   To express in mammalian cells a lactate FRET sensor negative control.

   Depositor

   Luis Felipe Barros

   Article

   Baeza-Lehnert et al Methods Mol Biol. 2020;2184:19-30. doi:

   Insert

   Dead-Laconic
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Change Histidine 151 to Aspartic Acid of Escheric…

   Promoter

   CMV Promoter

   Available Since

   Dec. 19, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3.1-Hyg-NLS.EGFP.P2A.Cherry.CAAX

   Plasmid

   #52421

   Purpose

   Expresses a polyprotein cleaved by peptide 2A into nuclear GFP and membrane located mCherry

   Depositor

   Bertrand Collet

   Insert

   Fusion NLS-EGFP-P2A-Cherry-CAAX
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   April 3, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3.1 3xF-dyskerin

   Plasmid

   #126870

   Purpose

   Expresses 3xFlag dsykerin

   Depositor

   Kathleen Collins

   Article

   Egan et al Mol Cell Biol. 2010 Jun;30(11):2775-86.

   Insert

   Dyskerin
   

   Use

   Tags

   3xFlag

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Aug. 12, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3.1/hChR2-EYFP

   Plasmid

   #20939

   Purpose

   Mammalian expression of humanized ChR2 fused to EYFP

   Depositor

   Karl Deisseroth

   Article

   Zhang et al Nature. 2007 Apr 5. 446(7136):633-9.

   Insert

   channelrhodopsin-2
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   May 13, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCDNA5/FRT/TO_CEP41_HaloTag9_T2A_EGFP

   Plasmid

   #169328

   Purpose

   Expression of HaloTag9 located at the microtubules of mammalian cells

   Depositor

   Kai Johnsson

   Article

   Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

   Insert

   CEP41-HaloTag9 (CEP41 Human)
   

   Use

   Tags

   HaloTag9

   Expression

   Mammalian

   Mutation

   HaloTag9 = HaloTag7-Q165H-P174R

   Promoter

   Available Since

   Nov. 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3 proHB-EGF NC

   Plasmid

   #11605

   Depositor

   Rosalyn Adam

   Article

   Kim et al Cancer Res. 2005 Sep 15. 65(18):8242-9.

   Insert

   HB-EGF (HBEGF Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Non-cleavable HB-EGF: Pro149 to Thr160 replaced w…

   Promoter

   Available Since

   April 26, 2006

   Availability

   Academic Institutions and Nonprofits only

10. pcDNA3.3-3XFLAG-AGO2

    Plasmid

    #136687

    Purpose

    Expresses human AGO2 with an N-terminal 3XFLAG tag in mammalian cells (using a CMV promoter)

    Depositor

    David Bartel

    Article

    McGeary et al Science. 2019 Dec 20;366(6472). pii: sci

    Insert

    argonaute RISC Catalytic Component 2 (AGO2 Human)
    

    Use

    Tags

    3XFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    March 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. hKCNQ3(WT).ires.mCherry-pcDNA3

    Plasmid

    #204362

    Purpose

    Heterologous expression in mammalian cell lines or Xenopus oocyte (T7 RNA pol)

    Depositor

    Aguan Wei

    Article

    Wei et al J Neurophysiol. 2022 Jul 1;128(1):40-61.

    Insert

    KCNQ3 (KCNQ3 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV/T7 RNApol

    Available Since

    Aug. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA3 HA-humanTRAF3 (1098)

    Plasmid

    #66927

    Purpose

    Mammalian Expression of TRAF3

    Depositor

    Michael Karin

    Insert

    TRAF3 (TRAF3 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Aug. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCDNA5 TEX264wt-cGFP

    Plasmid

    #220211

    Purpose

    Mammalian expression of wild type TEX264 fused at C-terminus to GFP

    Depositor

    Kristijan Ramadan

    Article

    Fielden et al Nat Commun. 2020 Mar 9;11(1):1274. doi:

    Insert

    TEX264 (TEX264 Human)
    

    Use

    Tags

    Strep-strep-HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV/TetO

    Available Since

    June 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA3.1-HA-mSTING (V154M)

    Plasmid

    #214159

    Purpose

    Transient expression of gain-of-function STING mutant

    Depositor

    Nan Yan

    Article

    Dobbs et al Cell Host Microbe. 2015 Aug 12;18(2):157

    Insert

    STING (V154M) (Sting1 Mouse)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    N153S

    Promoter

    CMV

    Available Since

    March 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA3-GFPLaminA-L530P

    Plasmid

    #32710

    Depositor

    Wendy Bickmore

    Article

    Gilchrist et al BMC Cell Biol. 2004 Dec 13;5(1):46.

    Insert

    Lamin A (LMNA Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    L530P

    Promoter

    CMV

    Available Since

    Jan. 27, 2012

    Availability

    Academic Institutions and Nonprofits only

16. pcDNA3.1 CLN3-GFP

    Plasmid

    #78110

    Purpose

    Mammalian expression of human CLN3 with C terminal GFP fusion

    Depositor

    Thomas Braulke

    Insert

    CLN3 (CLN3 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    June 20, 2016

    Availability

    Academic Institutions and Nonprofits only

17. FLAG-3C-sGFP1-10_pcDNA3.1(-)

    Plasmid

    #182931

    Purpose

    For expression of membrane protein with N-terminal FLAG tag and C-terminal split GFP1-10 fragment

    Depositor

    William Weis

    Article

    Bruguera et al J Biol Chem. 2022 Jan 21:101628. doi: 10

    Type

    Empty backbone

    Use

    Tags

    3C-cleavable split GFP 1-10, FLAG tag, and HA sig…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    April 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA3-ExRai-AktAR

    Plasmid

    #118462

    Purpose

    cpGFP-based excitation-ratiometric biosensor for monitoring Akt/Protein Kinase B activity.

    Depositor

    Jin Zhang

    Article

    Mehta et al Nat Cell Biol. 2018 Sep 24. pii: 10.1038

    Insert

    ExRai-AktAR
    

    Use

    Tags

    6xHIS, T7 tag (gene 10 leader), and Xpress (TM) t…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Dec. 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA3-His:PYL1:nls:VPR

    Plasmid

    #135988

    Purpose

    Expresses the PYL1 abscisic acid-inducible domain fused to the VPR transcriptional activation domain in mammalian cells

    Depositor

    Roman Jerala

    Article

    Lebar et al Nat Chem Biol. 2020 Jan 6. pii: 10.1038/

    Insert

    PYL1 domain fused to the VPR activation domain
    

    Use

    Tags

    His

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Feb. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pcDNA3-HA-CUL1 K720A

    Plasmid

    #19939

    Depositor

    Yue Xiong

    Article

    Furukawa et al Mol Cell Biol. 2000 Nov . 20(21):8185-97

    Insert

    cullin 1 K720A (CUL1 Human)
    

    Use

    Tags

    HA2

    Expression

    Mammalian

    Mutation

    CUL1 with a NEDD8 modification mutant

    Promoter

    Available Since

    April 17, 2009

    Availability

    Academic Institutions and Nonprofits only