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Plasmid#90168Purposecontains yrk specific gene promoter driving expression of eGFPDepositorInsertyrkP1Egfp (yrk Zebrafish)
UseTagsEGFPExpressionBacterialMutationPromoterAvailable sinceMay 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pT2-cryR;snx5P1Egfp
Plasmid#90166Purposecontains snx5 specific gene promoter driving expression of eGFPDepositorInsertsnx5P1Egfp (snx5 Zebrafish)
UseTagsEGFPExpressionBacterialMutationPromoterAvailable sinceMay 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pT2-cryR;yrkCE1-P1Egfp
Plasmid#90156Purposecontains yrk enhancer sequence, and yrk specific gene promoter driving expression of eGFPDepositorInsertyrkE1P1Egfp (yrk Zebrafish)
UseTagsEGFPExpressionBacterialMutationPromoterAvailable sinceMay 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
pT2-cryR;snx5CE1-P1Egfp
Plasmid#90152Purposecontains snx5 enhancer sequence, and snx5 specific gene promoter driving expression of eGFPDepositorInsertsnx5E1P1Egfp (snx5 Zebrafish)
UseTagsEGFPExpressionBacterialMutationPromoterAvailable sinceMay 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
pT2-cryR;sheCE1-P1Egfp
Plasmid#90150Purposecontains She enhancer sequence, and She specific gene promoter driving expression of eGFPDepositorInsertsheE1P1Egfp (she Zebrafish)
UseTagsEGFPExpressionBacterialMutationPromoterAvailable sinceMay 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
pT2-cryR;yrkCE1-basEgfp
Plasmid#90142Purposecontains yrk enhancer sequence and a basal promoter driving expression of eGFPDepositorInsertyrkE1basEgfp (yrk Zebrafish)
UseTagsEGFPExpressionBacterialMutationPromoterAvailable sinceMay 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
pT2-cryR;tmem88aCE1-basEgfp
Plasmid#90135Purposecontains tmem88a enhancer sequence and a basal promoter driving expression of eGFPDepositorInserttmem88aE1basEgfp (tmem88a Zebrafish)
UseTagsEGFPExpressionBacterialMutationPromoterAvailable sinceMay 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
pENTR221-WNT1
Plasmid#79499PurposeMultiSite Gateway entry clones, second fragment (attL5, attL2) Human WNT1 CDSDepositorInsertWNT1 (WNT1 Human)
UseMultisite gateway entry cloneTagsExpressionMutationPromoterno PromoterAvailable sinceMay 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pPD3cC
Plasmid#86972PurposeExpresses PCNA3 variant (R112C/T180C) fused phosphite dehydrogenase and P450cam in E. coliDepositorInsertPCNA3 variant (R112C/T180C) fused to phosphite dehydrogenase and P450cam
UseTagsHis6 tagExpressionBacterialMutationE175A/A176R in PTDH, N106R/R112C/T180C in PCNA3, …PromoterT7 promoterAvailable sinceMarch 15, 2017AvailabilityAcademic Institutions and Nonprofits only -
pTIM
Plasmid#81198PurposepTIM in vitro transcription backbone plasmid with two multiple cloning sites and two Rho-independent transcriptional terminators.DepositorTypeEmpty backboneUseUnspecifiedTagsExpressionMutationPromoterAvailable sinceDec. 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET23a-MmUBB(SnaBI)
Plasmid#69558PurposeIn vitro transcription/translation (T7)DepositorInsertUBB (Ubb Mouse)
UseTagsExpressionBacterialMutationThe two last codons (TATTAA; encoding the last ty…PromoterT7Available sinceDec. 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET23a-MmUBB(Y305P)
Plasmid#69557PurposeBacterial expression; in vitro transcription/translation (T7)DepositorInsertUBB (Ubb Mouse)
UseTagsExpressionBacterialMutationCodon TAT encoding tyrosine 305 was changed to CC…PromoterT7Available sinceDec. 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET12a-AL-09 FL Q42K C214S
Plasmid#47089DepositorInsertAL-09 FL Q42K C214S (IGKV3-20 Human)
UseTagsompTExpressionBacterialMutationQ42K C214SPromoterT7Available sinceAug. 2, 2013AvailabilityAcademic Institutions and Nonprofits only -
pET12a-AL-09 FL I34N C214S
Plasmid#47088DepositorInsertAL-09 FL I34N C214S (IGKV3-20 Human)
UseTagsompTExpressionBacterialMutationI34N C214SPromoterT7Available sinceAug. 2, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCR2.1-SNORD116-3
Plasmid#45891DepositorInsertSNORD115 with 80nt from intron sequence but without flanking exons (SNORD116-3 Human)
UseUnspecifiedTagsExpressionMutationPromoterAvailable sinceJuly 29, 2013AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-Lyn-FRB-DMVF-181-N
Plasmid#87609PurposeFRB-tagged N-terminal Dronpa fragment for BiFC; localizes to plasma membrane.DepositorInsertLyn-FRB-DMVF181-N
UseTagsN-terminal targeting sequence from Lyn kinaseExpressionMammalianMutationPromoterCMVAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-FKBP-DMVF-181-C
Plasmid#87611PurposeFKBP-tagged C-terminal Dronpa fragment for BiFC.DepositorInsertFKBP-DMVF181-C
UseTags6xHis, T7 tag (gene 10 leader), and Xpress(TM) ta…ExpressionMammalianMutationPromoterCMVAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
pTorPE-Y-GECO2s
Plasmid#105067PurposeA yellow fluorescent Ca2+ indicator variant with high binding affinity and slow kinetics for expression in bacterial cellsDepositorInsertY-GECO2s
UseTags6x His tagExpressionBacterialMutationPromoterpBADAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
pTorPE-Y-GECO2.1s
Plasmid#105068PurposeA yellow fluorescent Ca2+ indicator variant with high binding affinity and slow kinetics for expression in bacterial cellsDepositorInsertY-GECO2.1s
UseTags6x His tagExpressionBacterialMutationPromoterpBADAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
pTol2-nbt-mTagBFP2-P2A-mScarlet
Plasmid#196115Purposezebrafish expression of nbt-mTagBFP2-P2A-mScarletDepositorInsertnbt-mTagBFP2-P2A-mScarlet
UseZebrafish expressionTagsExpressionMutationPromoterAvailable sinceMarch 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCW57.1 Lysosomal-METRIQ
Plasmid#135401PurposeExpresses lysosomal-METRIQ (DNAse II-sfGFP together with cytosolic RFP) to measure lysosomal activityDepositorInsertDNAse II alpha (DNASE2 Human)
UseLentiviralTagsT2A, mCherry, and sfGFPExpressionMammalianMutationSingle-nucleotide polymorphism C432TPromoterAvailable sinceFeb. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
epiCRISPR
Plasmid#135960PurposeExpresses SpCas9, and cloning backbone for sgRNADepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterAvailable sinceMay 5, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-FLEXfrt-SaCas9-U6-sgRNA
Plasmid#124845PurposeVector for FLP-dependent expression of SaCas9 with gRNADepositorInsertSauCas9 gRNA scaffold
UseAAV, CRISPR, and Mouse TargetingTagsExpressionMutationPromoterAvailable sinceMay 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
N-BLa 1.2
Plasmid#88999PurposeBLaTM, a genetic tool to measure homo- and heterotypic transmembrane helix-helix interactions. Plasmid encoding the 1.2 fusionprotein containing the N-terminal fragment of the split β-lactamase.DepositorInsertN-BLa 1.2 fusion protein
UseTagsFlagExpressionBacterialMutationPromoteraraBADAvailable sinceMay 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
C-BLa 1.2
Plasmid#89000PurposeBLaTM, a genetic tool to measure homo- and heterotypic transmembrane helix-helix interactions. Plasmid encoding the 1.2 fusionprotein containing the C-terminal fragment of the split β-lactamase.DepositorInsertC-BLa 1.2 fusion protein
UseTagsFlagExpressionBacterialMutationPromoteraraBADAvailable sinceMay 2, 2017AvailabilityAcademic Institutions and Nonprofits only -
3xFLAG-Halo-Dyskerin
Plasmid#207578PurposeCRISPR-Cas9 genome editing encoding a FLAG-HaloTag at the endogenous dyskerin locusDepositorInsertHaloTag DKC1
UseTagsHaloTagExpressionMammalianMutationPromoterCMVAvailable sinceApril 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV-FLEX-SaCas9-U6-sgSlc18a2
Plasmid#124849PurposeMutagenesis of Slc18a2DepositorInsertSlc18a2 (Slc18a2 Mouse)
UseAAV, CRISPR, and Mouse TargetingTagsExpressionMutationPromoterAvailable sinceMay 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV-FLEXfrt-SaCas9-U6-sgSlc18a2
Plasmid#124862PurposeMutagenesis of Slc18a2DepositorInsertSlc18a2 (Slc18a2 Mouse)
UseAAV, CRISPR, and Mouse TargetingTagsExpressionMutationPromoterAvailable sinceMay 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
MAC_C_TYRO3
Plasmid#187793PurposeMAC-tagged gene expression of human TYRO3DepositorInsertTYRO3 (TYRO3 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceSept. 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSCM001
Plasmid#124605PurposeExpresses mCherry-pHluorin fusion protein in E. coli, a fluorescent reporter for cytoplasmic pHDepositorInsertmCherry-pHluorin
UseTagsmCherry-(Gly-Gly-Ser)x2-pHluorin translational fu…ExpressionBacterialMutationPromoterpDABAvailable sinceMay 14, 2019AvailabilityAcademic Institutions and Nonprofits only