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Showing: 4281 - 4320 of 8321 results

  1. MLYCD

    Plasmid
    #39081
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    MLYCD (MLYCD Human)
    Use
    Tags
    His Tev
    Expression
    Bacterial
    Mutation
    Surface mutant for crystallisation E19A, K20A, E2…
    Promoter
    Available since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  2. pCB32

    Plasmid
    #111911
    Purpose
    Expresses gRNA to target Nte1 locus and has HygR marker
    Depositor
    Yi Tang
    Insert
    gRNA cassette targeting Nte1 locus
    Use
    CRISPR
    Tags
    Expression
    Bacterial and Yeast
    Mutation
    Promoter
    SNR52p
    Available since
    Aug. 13, 2018
    Availability
    Academic Institutions and Nonprofits only
    111911-plasmid-map-sequence-id-218313

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  3. R26-CAG-loxP-FNF-bpA-3pA-loxP-td-sfGFP

    Plasmid
    #180151
    Purpose
    td-sfGFP cre reporter with Rosa26 recombineering homology arms
    Depositor
    Mario Capecchi
    Insert
    td-sfGFP
    Use
    Cre/Lox and Mouse Targeting
    Tags
    Expression
    Bacterial and Mammalian
    Mutation
    Promoter
    Available since
    Feb. 25, 2022
    Availability
    Academic Institutions and Nonprofits only
    180151-plasmid-map-sequence-id-355316

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  4. pZC11 (pFA6a-TEV-6xGly-V5-KanMX)

    Plasmid
    #44097
    Depositor
    Tim Formosa
    Type
    Empty backbone
    Use
    Pcr-based yeast c-terminal tagging
    Tags
    6xGly linker, ADH1 transcription terminator, KanM…
    Expression
    Mutation
    Promoter
    Available since
    April 1, 2013
    Availability
    Academic Institutions and Nonprofits only
    44097-plasmid-map-sequence-id-61694

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  5. px330_SMAD exon 9 gRNA

    Plasmid
    #68352
    Purpose
    px330 with gRNA towards SMAD exon 9. Cas9 is expressed from a CAG promoter.
    Depositor
    Jannik Elverløv-Jakobsen
    Insert
    SMAD exon 9 gRNA
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6
    Available since
    Oct. 5, 2015
    Availability
    Academic Institutions and Nonprofits only
    68352-plasmid-map-sequence-id-119624

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  6. pSP72 GSK3beta

    Plasmid
    #60125
    Purpose
    for transcription of GSK3beta
    Depositor
    Andrew McMahon
    Insert
    Glycogen synthase kinase 3 beta (Gsk3b Mouse)
    Use
    Cloning
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Jan. 13, 2015
    Availability
    Academic Institutions and Nonprofits only
    60125-plasmid-map-sequence-id-307046

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  7. pKG210

    Plasmid
    #69761
    Purpose
    Retroviral expression of CENP-T aa 1-375
    Depositor
    Iain Cheeseman
    Insert
    CENP-T aa 1-375 (CENPT Human)
    Use
    Retroviral
    Tags
    LacI-NLS and mCherry
    Expression
    Mammalian
    Mutation
    e.coli optimized sequence
    Promoter
    Available since
    Nov. 6, 2015
    Availability
    Academic Institutions and Nonprofits only

  8. HaloTag-G protein gamma-7

    Plasmid
    #67040
    Purpose
    G protein gamma-7 tagged with HaloTag on amino terminus
    Depositor
    Catherine Berlot
    Insert
    HaloTag-G protein gamma-7 (GNG7 Human)
    Use
    Tags
    HaloTag
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    July 30, 2015
    Availability
    Academic Institutions and Nonprofits only
    67040-plasmid-map-sequence-id-115582

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  9. pIC301

    Plasmid
    #69732
    Purpose
    Retroviral expression of Bub1
    Depositor
    Iain Cheeseman
    Insert
    Bub1 (BUB1 Human)
    Use
    Retroviral
    Tags
    eGFP-TEV-S
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Dec. 8, 2015
    Availability
    Academic Institutions and Nonprofits only

  10. pKG186

    Plasmid
    #63076
    Purpose
    Retroviral expression of EGFP-TEV-S-tag FANC-A for protein localization and affinity purification (LAP)
    Depositor
    Iain Cheeseman
    Insert
    FANC-A, IMAGE clone 5725803 (FANCA Human)
    Use
    Retroviral
    Tags
    EGFP and TEV-S
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    March 11, 2015
    Availability
    Academic Institutions and Nonprofits only
    63076-plasmid-map-sequence-id-104390

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  11. p-dCas9-JMJD2A-S288G-T289G-Hygro

    Plasmid
    #104418
    Purpose
    transient expression of dCas9-dJMJD2A fusion protein
    Depositor
    Stephan Beck
    Insert
    dJMJD2A
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    S288G-T289G
    Promoter
    CMV promoter
    Available since
    April 2, 2020
    Availability
    Academic Institutions and Nonprofits only
    104418-plasmid-map-sequence-id-204359

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  12. pCB100

    Plasmid
    #69746
    Purpose
    Retroviral expression of Centrin 3
    Depositor
    Iain Cheeseman
    Insert
    Centrin 3 (CETN3 Human)
    Use
    Retroviral
    Tags
    eGFP-TEV-S
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Nov. 4, 2015
    Availability
    Academic Institutions and Nonprofits only

  13. SPSB1

    Plasmid
    #38981
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    SPSB1 (SPSB1 Human)
    Use
    Tags
    6xHis-Tev
    Expression
    Bacterial
    Mutation
    Residues 24-233 of SPSB1 are present in this plas…
    Promoter
    Available since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only
    38981-plasmid-map-sequence-id-199971

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  14. pLP-mOlig2-ires-gfp

    Plasmid
    #49399
    Purpose
    mammalian bicistronic expression of mouse Olig2 and EGFP
    Depositor
    Randall McKinnon
    Insert
    Olig2 (Olig2 Mouse)
    Use
    Cre/Lox; Acceptor vector
    Tags
    EGFP and IRES
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    April 23, 2014
    Availability
    Academic Institutions and Nonprofits only
    49399-plasmid-map-sequence-id-316898

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  15. pBABEpuro-CRAF_S257L

    Plasmid
    #51125
    Purpose
    mammalian expression of wt CRAF S257L
    Depositor
    Matthew Meyerson
    Insert
    CRAF (RAF1 Human)
    Use
    Retroviral
    Tags
    Expression
    Mammalian
    Mutation
    S257L
    Promoter
    Available since
    Feb. 4, 2014
    Availability
    Academic Institutions and Nonprofits only
    51125-plasmid-map-sequence-id-77383

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  16. PBC002-A04

    Plasmid
    #58761
    Purpose
    bacterial expression of PDB 4PWY
    Depositor
    Cheryl Arrowsmith
    Insert
    PDB:4PWY (PEX6 Human)
    Use
    Tags
    His
    Expression
    Bacterial
    Mutation
    Bacterial expression for structure determination;…
    Promoter
    T7
    Available since
    Aug. 5, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits

  17. pE-SUMO CRYBB3

    Plasmid
    #80750
    Purpose
    Expression of human beta B3-crystallin in E. coli
    Depositor
    Larry David
    Insert
    CRYBB3 (CRYBB3 Human)
    Use
    Tags
    6xHIS and Smt3 SUMO
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    March 9, 2017
    Availability
    Academic Institutions and Nonprofits only
    80750-plasmid-map-sequence-id-152180

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  18. UXS1

    Plasmid
    #39162
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    UXS1 (UXS1 Human)
    Use
    Tags
    His Tev
    Expression
    Bacterial
    Mutation
    codon-optimized
    Promoter
    Available since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  19. FN 227

    Plasmid
    #50499
    Depositor
    Kenneth Yamada
    Insert
    FN1 fibronectin 1 (FN1 Human)
    Use
    Tags
    6X-His
    Expression
    Bacterial
    Mutation
    repeats 6-10; D1526E and R1524K
    Promoter
    T7
    Available since
    Jan. 7, 2014
    Availability
    Academic Institutions and Nonprofits only

  20. PA-TagRFP-Alpha-5-Integrin-12

    Plasmid
    #62791
    Purpose
    Localization: Focal Adhesions, Excitation:N/A, Emission:N/A
    Depositor
    Michael Davidson
    Insert
    Alpha5Integrin (ITGAV Human)
    Use
    Tags
    TagRFP
    Expression
    Mammalian
    Mutation
    none
    Promoter
    CMV
    Available since
    April 1, 2015
    Availability
    Industry, Academic Institutions, and Nonprofits

  21. ADH4

    Plasmid
    #38910
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    ADH4 (ADH4 Human)
    Use
    Tags
    His Tev
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  22. pLEX307-CTSL-blast

    Plasmid
    #158467
    Purpose
    Constitutive lentiviral expression of human CTSL.
    Depositor
    Sho Iketani, Alejandro Chavez
    Insert
    CTSL (CTSL Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Aug. 10, 2020
    Availability
    Academic Institutions and Nonprofits only
    158467-plasmid-map-sequence-id-308152

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  23. mEmerald-Enconsin-N-18

    Plasmid
    #62754
    Purpose
    Localization: Microtubule, N1 Cloning Vector, Excitation:487, Emission:509
    Depositor
    Michael Davidson
    Insert
    Enconsin (MAP7 Human)
    Use
    Tags
    mEmerald
    Expression
    Mammalian
    Mutation
    none
    Promoter
    CMV
    Available since
    March 10, 2015
    Availability
    Industry, Academic Institutions, and Nonprofits
    62754-plasmid-map-sequence-id-230170

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  24. pFLAG-6a-Ataxin3Q22-C14A

    Plasmid
    #22127
    Depositor
    Henry Paulson
    Insert
    Ataxin 3 (ATXN3 Human)
    Use
    Tags
    Flag
    Expression
    Mammalian
    Mutation
    C14A
    Promoter
    Available since
    Nov. 13, 2009
    Availability
    Academic Institutions and Nonprofits only

  25. TPK1

    Plasmid
    #32865
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    TPK1 (TPK1 Human)
    Use
    Tags
    His tag (with TEV cleavage)
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Jan. 6, 2012
    Availability
    Industry, Academic Institutions, and Nonprofits

  27. TMPRSS2 g4 + Cas9

    Plasmid
    #153018
    Purpose
    A guide RNA targeting TMPRSS2 in a lentiviral plasmid co-expressing Cas9 and TagBFP2
    Depositor
    Jason Sheltzer
    Insert
    Cas9 + TMPRSS2 gRNA
    Use
    CRISPR and Lentiviral
    Tags
    TagBFP2
    Expression
    Mutation
    Promoter
    Available since
    June 2, 2020
    Availability
    Academic Institutions and Nonprofits only
    153018-plasmid-map-sequence-id-298698

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  28. pDK714

    Plasmid
    #134230
    Purpose
    Mammalian expresion of tdTomato SKAP
    Depositor
    Iain Cheeseman
    Insert
    DK365 tdTomato (short SKAP) (KNSTRN Human)
    Use
    Tags
    tdTomato
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Feb. 18, 2020
    Availability
    Academic Institutions and Nonprofits only
    134230-plasmid-map-sequence-id-272270

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  29. PHIP

    Plasmid
    #39049
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    PHIP (PHIP Human)
    Use
    Tags
    His Tev
    Expression
    Bacterial
    Mutation
    codon-optimized
    Promoter
    Available since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  30. pT7-7 asyn S87A/S129A

    Plasmid
    #36063
    Depositor
    Hilal Lashuel
    Insert
    alpha-synuclein S87A/S129A (SNCA Human)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    S87A/S129A
    Promoter
    T7
    Available since
    July 13, 2012
    Availability
    Academic Institutions and Nonprofits only
    36063-plasmid-map-sequence-id-44519

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  31. H11-CAG-rox-FNF-bpA-3pA-rox-td-sfGFP

    Plasmid
    #180157
    Purpose
    td-sfGFP dre reporter with Hipp11 recombineering homology arms
    Depositor
    Mario Capecchi
    Insert
    td-sfGFP
    Use
    Mouse Targeting; Dre/rox
    Tags
    Expression
    Bacterial and Mammalian
    Mutation
    Promoter
    Available since
    Feb. 25, 2022
    Availability
    Academic Institutions and Nonprofits only
    180157-plasmid-map-sequence-id-355323

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  32. KG#865

    Plasmid
    #110912
    Purpose
    Coding sequence for attaching the Auxin Inducible Degron to the C-terminus of a protein followed by a 5X Myc tag with spacers
    Depositor
    Kenneth Miller
    Insert
    Auxin Inducible Degron-5X Myc-Spacers for C-terminal attachment
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    March 11, 2021
    Availability
    Academic Institutions and Nonprofits only
    110912-plasmid-map-sequence-id-212549

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  33. KG#883

    Plasmid
    #110913
    Purpose
    Coding sequence for attaching the a 5X Myc tag with spacers followed by the Auxin Inducible Degron to the N-terminus of a protein
    Depositor
    Kenneth Miller
    Insert
    5X Myc-Spacers-Auxin Inducible Degron for N-terminal attachment
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    March 11, 2021
    Availability
    Academic Institutions and Nonprofits only
    110913-plasmid-map-sequence-id-212540

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  34. pINDUCER20-FLAG-hMYF5 (#801)

    Plasmid
    #78334
    Purpose
    Doxycycline-inducible lentiviral overexpression of human MYF5 ORF with a FLAG tag
    Depositor
    Louis Kunkel, Matthew Alexander
    Insert
    MYF5 (MYF5 Human)
    Use
    Lentiviral
    Tags
    FLAG
    Expression
    Mutation
    Promoter
    TRE2
    Available since
    July 11, 2016
    Availability
    Academic Institutions and Nonprofits only

  35. pJK650

    Plasmid
    #72400
    Purpose
    Produces Escherichia coli glutamine synthetase with Y397F mutation that prevents adenylation (EcGlnA-Y397F)
    Depositor
    T. J. Kappock
    Insert
    glutamine synthetase (glnA Escherichia coli str. K-12 substr. MG1655))
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Mutated tyrosine-397 to phenylalanine to block ad…
    Promoter
    T7
    Available since
    March 21, 2016
    Availability
    Academic Institutions and Nonprofits only
    72400-plasmid-map-sequence-id-133994

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  36. pRK VSV Cortactin

    Plasmid
    #50727
    Purpose
    mammalian expression of VSV-tagged Cortactin
    Depositor
    Kenneth Yamada
    Insert
    Cortactin (CTTN Human)
    Use
    Tags
    2xVSV
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Jan. 7, 2014
    Availability
    Academic Institutions and Nonprofits only

  38. CAG-FNLS-T2A-EGFP-ires-puro

    Plasmid
    #121169
    Purpose
    CAG promoter driven expression of FNLS BE3 base editor, EGFP and Puromycin resistance.
    Depositor
    Timo Otonkoski
    Insert
    FNLS-T2A-EGFP-ires-puro
    Use
    CRISPR
    Tags
    T2A-EGFP-ires-puro
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    July 18, 2019
    Availability
    Academic Institutions and Nonprofits only
    121169-plasmid-map-sequence-id-235002

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  39. pLV-EF1a-V5-Bet1

    Plasmid
    #120243
    Purpose
    Expresses V5-tagged Bet1 in lentiviral vector
    Depositor
    Larry Gerace
    Insert
    Bet1 (Bet1 Mouse)
    Use
    Lentiviral
    Tags
    V5
    Expression
    Mutation
    Promoter
    EF1a
    Available since
    Jan. 9, 2019
    Availability
    Academic Institutions and Nonprofits only
    120243-plasmid-map-sequence-id-232790

    Enlarge

  40. H11-CAG-loxP-FNF-bpA-3pA-loxP-td-sfGFP

    Plasmid
    #180155
    Purpose
    td-sfGFP cre reporter with Hipp11 recombineering homology arms
    Depositor
    Mario Capecchi
    Insert
    td-sfGFP
    Use
    Cre/Lox and Mouse Targeting
    Tags
    Expression
    Bacterial and Mammalian
    Mutation
    Promoter
    Available since
    Feb. 25, 2022
    Availability
    Academic Institutions and Nonprofits only
    180155-plasmid-map-sequence-id-355320

    Enlarge

Showing: 4281 - 4320 of 8321 results