We narrowed to 5,270 results for: pcdna3.1

1. Kif1A(1-489)-DHFR-myc

   Plasmid

   #117833

   Purpose

   Constitutively active Kinesin-3 motor tagged with DHFR and myc

   Depositor

   Thomas Schwarz

   Article

   Gutnick et al Nat Cell Biol. 2019 Jun;21(6):768-777. d

   Insert

   Kif1A (1-489) (Kif1a Mouse)
   

   Use

   Tags

   DHFR and myc

   Expression

   Mutation

   only aa 1-489 (motor domain)

   Promoter

   CMV

   Available since

   June 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pcDNA-f:PGC1(delta CTD)

   Plasmid

   #1030

   Depositor

   Bruce Spiegelman

   Article

   Monsalve et al Mol Cell 2000 Aug;6(2):307-16.

   Insert

   PGC1a (Ppargc1a Mouse)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   delta CTD

   Promoter

   Available since

   Oct. 4, 2005

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. CoV2-N-M234I

   Plasmid

   #177955

   Purpose

   Transient mammalian expression of codon optimized SARS-CoV-2 Nucleocapsid (M234I). Used for generating RNA packaging virus-like particles.

   Depositor

   Jennifer Doudna

   Article

   Syed et al Science. 2021 Nov 4:eabl6184. doi: 10.11

   Insert

   SARS-CoV-2 Nucleocapsid (M234I) (N )
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   M234I

   Promoter

   Available since

   Nov. 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pcDNA-Cx43-Myc

   Plasmid

   #60693

   Purpose

   mammalian expression of Myc-tagged rat Cx43

   Depositor

   Parmender Mehta

   Article

   Johnson et al Mol Biol Cell. 2013 Mar;24(6):715-33. do

   Insert

   Cx43 (Gja1 Rat)
   

   Use

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 6, 2015

   Availability

   Academic Institutions and Nonprofits only

5. pcDNA-Prom1 D-5

   Plasmid

   #184017

   Purpose

   Mammalian Flag tagged expression clone for the mouse Prom1 splicing variant SV8 (GenBank accession BC028286). Deletion of exon 19a (amino acids 696-701), and deletion of amino acids 641 to 655.

   Depositor

   Peter Stoilov

   Article

   Matalkah et al PLoS One. 2022 Oct 10;17(10):e0274958. d

   Insert

   Prom1 SV8(-Ex19a) D-5 (Prom1 Mouse)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Deletion of exon 19a (amino acids 696-701), and d…

   Promoter

   Available since

   May 23, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pmyc-GFP-TNRC6A-del.GW-I+II+III-NES-mut

   Plasmid

   #42040

   Depositor

   Kumiko Ui-Tei

   Article

   Nishi et al RNA. 2013 Jan;19(1):17-35. doi: 10.1261/

   Insert

   TNRC6A (TNRC6A Human)
   

   Use

   Tags

   EGFP and Myc

   Expression

   Mammalian

   Mutation

   deleted aa457-532, aa716-772 and aa804-924, F951A…

   Promoter

   CMV

   Available since

   March 6, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. Myc-gamma2s

   Plasmid

   #118036

   Purpose

   GABAA receptor expression (gamma2 short subunit) Myc-tag near N-terminus

   Depositor

   Angel de Blas

   Article

   Christie et al Mol Cell Neurosci. 2006 Jan;31(1):1-14.

   Insert

   GABRG2 (Gabrg2 Rat)
   

   Use

   Tags

   cMyc epitope EQKLISEEDL inserted between fourth a…

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pJO427

   Plasmid

   #111139

   Purpose

   URT-type vector for Glu-mIκBα expression in Mammalian cells

   Depositor

   Alexander Varshavsky

   Article

   Piatkov et al Proc Natl Acad Sci U S A. 2014 Mar 4;111

   Insert

   flag-lacZ25-119-DHFR-UbK48R-Glu51-mIκBα (Ikbkb Mouse)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 4, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. Foxf2 pcDNA HisC

   Plasmid

   #122121

   Purpose

   Expression of Foxf2 in mammalian cells

   Depositor

   Paul Herring

   Article

   Hoggatt et al J Biol Chem. 2013 Oct 4;288(40):28477-87

   Insert

   Foxf2 (Foxf2 Mouse)
   

   Use

   Tags

   6xHis and Express

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. MAP4K3-GFP K45E

    Plasmid

    #21734

    Depositor

    L. Miguel Martins

    Article

    Lam et al Proc Natl Acad Sci U S A. 2009 Jul 8. ()

    Insert

    MAP4K3 (MAP4K3 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Mutation of the ATP-binding site (K45E). This ver…

    Promoter

    Available since

    June 22, 2010

    Availability

    Academic Institutions and Nonprofits only

11. Opto-GPR150

    Plasmid

    #106050

    Purpose

    Expression of a chimeric G-protein coupled receptor (Rhodopsin-GPR150; Opto-GPR150; E4)

    Depositor

    Harald Janovjak

    Article

    Morri et al Nat Commun. 2018 May 16;9(1):1950. doi:

    Insert

    Opto-GPR150 (GPR150 Human, Bovine)
    

    Use

    Tags

    Rhodopsin-1D4 and VSV-G

    Expression

    Mammalian

    Mutation

    Chimeric receptor protein

    Promoter

    CMV

    Available since

    Jan. 23, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. CoV2-N-D377Y

    Plasmid

    #177957

    Purpose

    Transient mammalian expression of codon optimized SARS-CoV-2 Nucleocapsid (D377Y). Used for generating RNA packaging virus-like particles.

    Depositor

    Jennifer Doudna

    Article

    Syed et al Science. 2021 Nov 4:eabl6184. doi: 10.11

    Insert

    SARS-CoV-2 Nucleocapsid (D377Y) (N )
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    D377Y

    Promoter

    Available since

    Nov. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pspCas9-ABE-3'UTR-sgRNA-2xMS2

    Plasmid

    #132554

    Purpose

    Vector plasmid expressing ABEmax and sgRNA scaffold with MS2 replacing the Tetraloop and loop 2

    Depositor

    Baisong Lu

    Article

    Lyu et al CRISPR J. 2021 Feb;4(1):69-81. doi: 10.1

    Inserts

    ABEmax
    sgRNA, with Tetraloop and loop 2 replaced by MS2 aptamer
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pJO428

    Plasmid

    #111141

    Purpose

    URT-type vector for Val-mIκBα expression in Mammalian cells

    Depositor

    Alexander Varshavsky

    Article

    Piatkov et al Proc Natl Acad Sci U S A. 2014 Mar 4;111

    Insert

    flag-lacZ25-119-DHFR-UbK48R-Val51-mIκBα (Ikbkb Mouse)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pTE3987

    Plasmid

    #137716

    Purpose

    Expresses dLbCas12a-RVRR-BE in mammalian cells.

    Depositor

    Ervin Welker

    Article

    Toth et al Nucleic Acids Res. 2020 Feb 28. pii: 576

    Insert

    dLbCas12a-RVRR-BE
    

    Use

    Tags

    6xHis, APOBEC-1, SV40 NLS, and UGI

    Expression

    Mammalian

    Mutation

    G532A, K538V, Y542R, K595R

    Promoter

    CMV

    Available since

    March 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. Myc-delta

    Plasmid

    #118037

    Purpose

    GABAA receptor expression (delta subunit) Myc-tag near N-terminus

    Depositor

    Angel de Blas

    Article

    Christie et al Mol Cell Neurosci. 2006 Jan;31(1):1-14.

    Insert

    GABRD (Gabrd Rat)
    

    Use

    Tags

    cMyc epitope EQKLISEEDL inserted between fourth a…

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pCDNA3-HA/Bmal L95E

    Plasmid

    #47344

    Purpose

    PcHA-Bmal backbone used for the above mutation. Ref.Genes & Dev 17:1921-1932 2003

    Depositor

    Joseph Takahashi

    Article

    Huang et al Science. 2012 Jul 13;337(6091):189-94. d

    Insert

    BMAL (Bmal1 Mouse)
    

    Use

    Tags

    Ha

    Expression

    Mammalian

    Mutation

    L95E

    Promoter

    CMV

    Available since

    Sept. 4, 2013

    Availability

    Academic Institutions and Nonprofits only

18. hMEF2A K403R

    Plasmid

    #118355

    Purpose

    This plasmid expresses human MEF2A K403R mutant. This construct cannot undergo sumoylation on K403.

    Depositor

    Lea Sistonen

    Article

    Hietakangas et al Proc Natl Acad Sci U S A. 2006 Jan 3;103

    Insert

    hMEF2A (MEF2A Human)
    

    Use

    Tags

    myc and 6xHIS

    Expression

    Mammalian

    Mutation

    K403R

    Promoter

    Available since

    Dec. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pcDNA-p160(1-200)

    Plasmid

    #43

    Depositor

    Bruce Spiegelman

    Article

    Fan et al Genes Dev 2004 Feb 1;18(3):278-89. Epub

    Insert

    p160 myb binding protein (Mybbp1a Mouse)
    

    Use

    Tags

    myc

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 24, 2006

    Availability

    Academic Institutions and Nonprofits only

20. pcDNA-p160(400-580)

    Plasmid

    #45

    Depositor

    Bruce Spiegelman

    Article

    Fan et al Genes Dev 2004 Feb 1;18(3):278-89. Epub

    Insert

    p160 myb binding protein (Mybbp1a Mouse)
    

    Use

    Tags

    myc

    Expression

    Mammalian

    Mutation

    expresses only aa400-580

    Promoter

    Available since

    May 24, 2006

    Availability

    Academic Institutions and Nonprofits only

21. pcDNA-p160(200-400)

    Plasmid

    #44

    Depositor

    Bruce Spiegelman

    Article

    Fan et al Genes Dev 2004 Feb 1;18(3):278-89. Epub

    Insert

    p160 myb binding protein (Mybbp1a Mouse)
    

    Use

    Tags

    myc

    Expression

    Mammalian

    Mutation

    Expresses only aa200-400

    Promoter

    Available since

    July 21, 2005

    Availability

    Academic Institutions and Nonprofits only

22. pcDNA3 RL23Cm6

    Plasmid

    #109367

    Purpose

    Human rod opsin chimera with intracellular loops 2-3 and C-terminus from mGluR6

    Depositor

    Robert Lucas

    Article

    Ballister et al BMC Biol. 2018 Jan 16;16(1):10. doi: 10.

    Insert

    Rod opsin-mGluR6 chimera (RHO, GRM6 Human)
    

    Use

    Tags

    1D4

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. CoV2-N-P13L

    Plasmid

    #177946

    Purpose

    Transient mammalian expression of codon optimized SARS-CoV-2 Nucleocapsid (P13L). Used for generating RNA packaging virus-like particles.

    Depositor

    Jennifer Doudna

    Article

    Syed et al Science. 2021 Nov 4:eabl6184. doi: 10.11

    Insert

    SARS-CoV-2 Nucleocapsid (P13L) (N )
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    P13L

    Promoter

    Available since

    Nov. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. CoV2-N-P80R

    Plasmid

    #177947

    Purpose

    Transient mammalian expression of codon optimized SARS-CoV-2 Nucleocapsid (P80R). Used for generating RNA packaging virus-like particles.

    Depositor

    Jennifer Doudna

    Article

    Syed et al Science. 2021 Nov 4:eabl6184. doi: 10.11

    Insert

    SARS-CoV-2 Nucleocapsid (P80R) (N )
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    P80R

    Promoter

    Available since

    Nov. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. CoV2-N-S235F

    Plasmid

    #177956

    Purpose

    Transient mammalian expression of codon optimized SARS-CoV-2 Nucleocapsid (S235F). Used for generating RNA packaging virus-like particles.

    Depositor

    Jennifer Doudna

    Article

    Syed et al Science. 2021 Nov 4:eabl6184. doi: 10.11

    Insert

    SARS-CoV-2 Nucleocapsid (S235F) (N )
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    S235F

    Promoter

    Available since

    Nov. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. CNC3 (CASANOVA-C3)

    Plasmid

    #137191

    Purpose

    Expresses CNC3 (CASANOVA-C3), a blue light dependent NmeCas9 inhibitory protein

    Depositor

    Dominik Niopek

    Article

    Hoffmann et al Nucleic Acids Res. 2021 Mar 18;49(5):e29

    Insert

    CNC3 (CASANOVA-C3)
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. 1029-AIDx-CP

    Plasmid

    #135341

    Purpose

    Expression of circular permutant nSpyCas9 at amino acid position 1029 encoding truncated human cytosine deaminase, hAIDx, at the C-terminus.

    Depositor

    Alex Hewitt

    Article

    Nguyen Tran et al Nat Commun. 2020 Sep 25;11(1):4871. doi:

    Insert

    CP 1029 nSpyCas9-hAIDx
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    Available since

    Oct. 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. FLAG-FLJ23445

    Plasmid

    #15429

    Depositor

    Ronald Conaway, Joan Conaway

    Article

    Sato et al J Biol Chem. 2003 Apr 25. 278(17):15123-

    Insert

    FLJ23445 (Thoc7 Mouse)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 10, 2007

    Availability

    Academic Institutions and Nonprofits only

29. Opto-GPR173

    Plasmid

    #106058

    Purpose

    Expression of a chimeric G-protein coupled receptor (Rhodopsin-GPR173; Opto-GPR173; E12)

    Depositor

    Harald Janovjak

    Article

    Morri et al Nat Commun. 2018 May 16;9(1):1950. doi:

    Insert

    Opto-GPR173 (GPR173 Human, Bovine)
    

    Use

    Tags

    Rhodopsin-1D4 and VSV-G

    Expression

    Mammalian

    Mutation

    Chimeric receptor protein

    Promoter

    CMV

    Available since

    Jan. 23, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. Opto-GPR31

    Plasmid

    #106014

    Purpose

    Expression of a chimeric G-protein coupled receptor (Rhodopsin-GPR31; Opto-GPR31; B4)

    Depositor

    Harald Janovjak

    Article

    Morri et al Nat Commun. 2018 May 16;9(1):1950. doi:

    Insert

    Opto-GPR31 (GPR31 Human, Bovine)
    

    Use

    Tags

    Rhodopsin-1D4 and VSV-G

    Expression

    Mammalian

    Mutation

    Chimeric receptor protein

    Promoter

    CMV

    Available since

    Jan. 23, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pGAL4-DBD-TBX1wt-IDR

    Plasmid

    #145254

    Purpose

    Expresses fusion of GAL4 DNA-binding domain and TBX1wt IDR

    Depositor

    Denes Hnisz

    Article

    Basu et al Cell. 2020 May 28;181(5):1062-1079.e30.

    Insert

    TBX1wt-IDR (TBX1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. Myc-PCDHGC5

    Plasmid

    #119935

    Purpose

    Protocadherin gammaC5 expression (Myc-tag is at the linker between EC1 and EC2 extracellular cadherin repeats)

    Depositor

    Angel de Blas

    Article

    Li et al J Neurosci. 2012 Aug 22;32(34):11780-97.

    Insert

    PCDHGC5 (Pcdhgc5 Rat)
    

    Use

    Tags

    9E10 cMyc epitope (EQKLISEEDL) was inserted betwe…

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pCDNA3-HA/Bmal I317D

    Plasmid

    #47347

    Purpose

    PcHA-Bmal backbone used for the above mutation. Ref.Genes & Dev 17:1921-1932 2003

    Depositor

    Joseph Takahashi

    Article

    Huang et al Science. 2012 Jul 13;337(6091):189-94. d

    Insert

    BMAL (Bmal1 Mouse)
    

    Use

    Tags

    Ha

    Expression

    Mammalian

    Mutation

    I317D

    Promoter

    CMV

    Available since

    Jan. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

34. Opto-GPR25

    Plasmid

    #106011

    Purpose

    Expression of a chimeric G-protein coupled receptor (Rhodopsin-GPR25; Opto-GPR25; B1)

    Depositor

    Harald Janovjak

    Article

    Morri et al Nat Commun. 2018 May 16;9(1):1950. doi:

    Insert

    Opto-GPR25 (GPR25 Human, Bovine)
    

    Use

    Tags

    Rhodopsin-1D4 and VSV-G

    Expression

    Mammalian

    Mutation

    Chimeric receptor protein

    Promoter

    CMV

    Available since

    Jan. 23, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pCDNA3-HA/Bmal L115E

    Plasmid

    #47345

    Purpose

    PcHA-Bmal backbone used for the above mutation. Ref.Genes & Dev 17:1921-1932 2003

    Depositor

    Joseph Takahashi

    Article

    Huang et al Science. 2012 Jul 13;337(6091):189-94. d

    Insert

    BMAL (Bmal1 Mouse)
    

    Use

    Tags

    Ha

    Expression

    Mammalian

    Mutation

    L115E

    Promoter

    CMV

    Available since

    Sept. 4, 2013

    Availability

    Academic Institutions and Nonprofits only

36. PCDNA3-N3FLAG-MYB-(1-327)

    Plasmid

    #105612

    Purpose

    transient express MYB N terminal fragment with 3*FLAG tag at N terminal in HEK 293T cells

    Depositor

    Christopher Vakoc

    Article

    Xu et al Cancer Cell. 2018 Jan 8;33(1):13-28.e8.

    Insert

    N terminal fragment of MYB (mouse), amino acids 1-327 (Myb Mouse)
    

    Use

    Tags

    3*FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. PCDNA3-N3FLAG-MYB-TAD(251-327)

    Plasmid

    #105611

    Purpose

    transient express MYB TAD fragment with 3*FLAG tag at N terminal in HEK 293T cells

    Depositor

    Christopher Vakoc

    Article

    Xu et al Cancer Cell. 2018 Jan 8;33(1):13-28.e8.

    Insert

    TAD fragment of MYB (mouse), amino acids 251-327 (Myb Mouse)
    

    Use

    Tags

    3*FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. PCDNA3-N3FLAG-MYB-DBD(1-200)

    Plasmid

    #105610

    Purpose

    transient express MYB DBD fragment with 3*FLAG tag at N terminal in HEK 293T cells

    Depositor

    Christopher Vakoc

    Article

    Xu et al Cancer Cell. 2018 Jan 8;33(1):13-28.e8.

    Insert

    DBD fragment of MYB (mouse), amino acids 1-200 (Myb Mouse)
    

    Use

    Tags

    3*FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. PCDNA3-N3HA-TAF12-HFD(50-130)

    Plasmid

    #105613

    Purpose

    transient express TAF12 HFD with 3* HA tag at N terminal in HEK 293T cells

    Depositor

    Christopher Vakoc

    Article

    Xu et al Cancer Cell. 2018 Jan 8;33(1):13-28.e8.

    Insert

    TAF12 HFD amino acid 50-130 with HA tag (Taf12 Mouse)
    

    Use

    Tags

    3*HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pCDNA3-HA/Bmal W427A

    Plasmid

    #47349

    Purpose

    PcHA-Bmal backbone used for the above mutation. Ref.Genes & Dev 17:1921-1932 2003

    Depositor

    Joseph Takahashi

    Article

    Huang et al Science. 2012 Jul 13;337(6091):189-94. d

    Insert

    BMAL (Bmal1 Mouse)
    

    Use

    Tags

    Ha

    Expression

    Mammalian

    Mutation

    W427A

    Promoter

    CMV

    Available since

    Jan. 7, 2014

    Availability

    Academic Institutions and Nonprofits only