We narrowed to 5,822 results for: pcas

1. pCAG-lox-roxSTOProx-tRFP-pA-lox

   Plasmid

   #196888

   Purpose

   Dre-activated Cre-inactivated expression of turbo (t)RFP reporter.

   Depositor

   Frank Bradke

   Article

   Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

   Insert

   turbo (t)RFP
   

   Use

   Cre/Lox; Dre/rox

   Expression

   Mammalian

   Promoter

   Quimeric CAG

   Available Since

   May 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pSpCas9(BB)-2A-Puro(PX459)-GJA1 sgRNA

   Plasmid

   #164471

   Purpose

   Expresses sgRNA targeting human GJA1 locus

   Depositor

   Markus Kuehn

   Article

   Cheng et al Stem Cells. 2022 Mar 9. pii: 6545973. do

   Insert

   Gap junction alpha-1 (GJA1) (GJA1 Human)
   

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   May 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pEN716 - pCAGGS-eGFP-3Xnls-bpA

   Plasmid

   #156435

   Purpose

   For transient expression of nuclear eGFP in mammalian cells

   Depositor

   Elphege Nora

   Article

   Nora et al Nat Commun. 2020 Nov 5;11(1):5612. doi:

   Insert

   eGFP
   

   Expression

   Mammalian

   Mutation

   none

   Available Since

   Sept. 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pSpCAS9 wt (BB)-2A-GFP targeting human TRIM21

   Plasmid

   #138295

   Purpose

   gRNA for CRISPR/Cas9 knockout of human TRIM21

   Depositor

   Gaudenz Danuser

   Article

   Park et al Nature. 2020 Feb 12. pii: 10.1038/s41586

   Insert

   gRNA TRIM21 (TRIM21 Human)
   

   Use

   CRISPR

   Promoter

   U6

   Available Since

   June 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pPW3754 : pUC-SpCas9_gRNA-HsIRE1-Cterm

   Plasmid

   #185676

   Purpose

   SpCas9 and gRNA targeting the C-terminus of HsIRE1

   Depositor

   Peter Walter

   Article

   Belyy et al Elife. 2022 Jun 22;11:e74342. doi: 10.75

   Insert

   ERN1 gRNA (ERN1 Human)
   

   Use

   CRISPR

   Available Since

   March 14, 2023

   Availability

   Academic Institutions and Nonprofits only

6. pCAG-TWIN-STREP-FLAG-NPRL3

   Plasmid

   #164984

   Purpose

   NPRL3 expressing vector for mammalian cells

   Depositor

   Roberto Zoncu

   Article

   Lawrence et al Science. 2019 Nov 22;366(6468):971-977.

   Insert

   NPRL3 (NPRL3 Human)
   

   Tags

   2xSTREP FLAG

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   March 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. peSpCas9(1.1)-2×sgRNA (IFT88, donor)

   Plasmid

   #80769

   Purpose

   Expresses eSpCas9(1.1) and two sgRNAs. The first gRNA targets human IFT88 and the second gRNA targets a pDonor-tBFP-NLS-Neo (Universal).

   Depositor

   Kazuhisa Nakayama

   Article

   Katoh et al Mol Biol Cell. 2017 Feb 8. pii: mbc.E17-

   Insert

   IFT88 gRNA#1 (IFT88 Human)
   

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   March 15, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCalpha EV (PKA catalytic subunit Calpha)

   Plasmid

   #15310

   Depositor

   Stanley McKnight

   Article

   Uhler et al J Biol Chem. 1987 Nov 5. 262(31):15202-7

   Insert

   PRKACA (Prkaca Mouse)
   

   Expression

   Mammalian

   Available Since

   July 6, 2007

   Availability

   Academic Institutions and Nonprofits only

9. pU6-PspCas13b-non-targeting (pJK5)

   Plasmid

   #229052

   Purpose

   Cloning backbone for PspCas13b crRNA (U6 promoter)

   Depositor

   S Chul Kwon

   Article

   Cheng et al EMBO Rep. 2025 Feb 26. doi: 10.1038/s443

   Type

   Empty backbone

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   Feb. 26, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. p2T-cmv-SpCas9-TadDE-BlastR

    Plasmid

    #193850

    Purpose

    Express TadDE in mammalian cells with BlastR selection, Tol2 integration

    Depositor

    David Liu

    Article

    Neugebauer et al Nat Biotechnol. 2022 Nov 10. pii: 10.103

    Insert

    TadDE
    

    Expression

    Mammalian

    Available Since

    Dec. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. PET-eSpCas9-plus-NLS-6xHis

    Plasmid

    #207380

    Purpose

    Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

    Insert

    eSpCas9-plus
    

    Use

    CRISPR

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    K848A, R1060A, amino acids 1005-1013 replaced wit…

    Promoter

    T7

    Available Since

    Sept. 25, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCAG-Golden-Gate-Esp3I-Destination

    Plasmid

    #51278

    Purpose

    CAG promoter-driven expression vector suitable for ligation of multiple inserts by Golden Gate Cloning using Esp3I

    Depositor

    Pawel Pelczar

    Article

    Hermann et al Nucleic Acids Res. 2014 Jan 9.

    Type

    Empty backbone

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    March 4, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCAGGS SARS-CoV-2 BA.4.6 Spike

    Plasmid

    #190013

    Purpose

    Expresses codon-optimized full length SARS-CoV-2 BA.4.6 Spike

    Depositor

    Marceline Côté

    Article

    Tauzin et al Cell Rep. 2023 Jan 31;42(1):111998. doi:

    Insert

    SARS-CoV-2 BA.4.6 Spike (S )
    

    Expression

    Mammalian

    Mutation

    Contains the following mutations: T19I, LPPA24S, …

    Promoter

    chicken β-actin promoter, CMV enhancer

    Available Since

    Sept. 7, 2022

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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14. pOTTC1124 - pAAV MeCP2 SpCas9(D10A)

    Plasmid

    #112719

    Purpose

    An AAV vector that expresses SpCas9 nickase under a neuronal cell promoter

    Depositor

    Brandon Harvey

    Article

    Back et al Neuron. 2019 Feb 8. pii: S0896-6273(19)3

    Insert

    SpCas9
    

    Use

    AAV

    Mutation

    D10A

    Promoter

    Mecp2

    Available Since

    March 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. p2T-cmv-SpCas9-TadCBEa-BlastR

    Plasmid

    #193847

    Purpose

    Express TadCBEa in mammalian cells with BlastR selection, Tol2 integration

    Depositor

    David Liu

    Article

    Neugebauer et al Nat Biotechnol. 2022 Nov 10. pii: 10.103

    Insert

    TadCBEa
    

    Expression

    Mammalian

    Available Since

    Dec. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCAGGS-PDGFRb559-562del-IRES2-EGFP

    Plasmid

    #204355

    Purpose

    Expresses a mutant PDGFRb gene (559-562 deletion).

    Depositor

    Hirofumi Nakatomi

    Article

    Shima et al Sci Transl Med. 2023 Jun 14;15(700):eabq

    Insert

    Platelet derived growth factor receptor beta (PDGFRB) (PDGFRB Human)
    

    Mutation

    559-562 deletion

    Available Since

    Sept. 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCAG-FLEX-fwd[Chrimson-tdT]

    Plasmid

    #59137

    Purpose

    Expression vector for Chrimson-tdTomato in a floxed/forward-reading, Cre-dependent manner (Cre turns gene off)

    Depositor

    Edward Boyden

    Article

    Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

    Insert

    Chrimson-tdTomato
    

    Use

    Cre/Lox

    Tags

    tdTomato

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Aug. 13, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCAG-EGFP/RFP-miR-Scrint

    Plasmid

    #19821

    Depositor

    Zuoshang Xu

    Article

    Qiu et al BMC Biotechnol. 2008 Oct 7;8:77. doi: 10

    Insert

    Scrambled miRNA
    

    Use

    Cre/Lox and RNAi

    Expression

    Mammalian

    Available Since

    Nov. 21, 2008

    Availability

    Academic Institutions and Nonprofits only

19. pspCas9-3'UTR-ST2-com-vector

    Plasmid

    #136269

    Purpose

    Expresses SpCas9 and sgRNA Scaffold

    Depositor

    Baisong Lu

    Article

    Lu et al CRISPR J. 2021 Mar 16. doi: 10.1089/cris

    Insert

    SpCas9 and sgRNA scaffold
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Available Since

    Nov. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. AAV pCAG-FLEX-mRuby3-WPRE

    Plasmid

    #99279

    Purpose

    Can be used to generate AAV virus that will express mRuby3 in the presence of Cre

    Depositor

    Rylan Larsen

    Insert

    mRuby3
    

    Use

    AAV and Cre/Lox

    Available Since

    Aug. 18, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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