We narrowed to 2,382 results for: T2A

1. PCDNA3-FlipYFP(TEV cleavage seq) T2A mCherry

   Plasmid

   #124493

   Purpose

   Expresses FlipYFP (TEV cleavage sequence) and T2A mCherry

   Depositor

   Xiaokun Shu

   Article

   Zhang et al J Am Chem Soc. 2019 Mar 1. doi: 10.1021/

   Insert

   FlipYFP(TEV cleavage seq) T2A mCherry
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA5/FRT/TO_Lifeact-HaloTag11-T2A-Tomm20-HaloTag7

   Plasmid

   #175526

   Purpose

   Co-expression of HaloTag11 (actin) and 7 (mitochondrial surface) in mammalian cells

   Depositor

   Kai Johnsson

   Article

   Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

   Insert

   Lifeact-HaloTag11 and Tomm20-HaloTag7
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   HaloTag11 = HaloTag7-M175W

   Promoter

   Available since

   Dec. 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCMVd1-NLS-GAL4-iLight2-VP16-T2A-mTagBFP2

   Plasmid

   #219548

   Purpose

   NLS-Gal4(1-65)-iLight2-VP16-T2A-mTagBFP2 construct expression in mammalian cells.

   Depositor

   Vladislav Verkhusha

   Article

   Baloban et al Protein Sci. 2024 May;33(5):e4993. doi:

   Insert

   NLS-Gal4(1-65)-iLight2-VP16-T2A-mTagBFP2
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   The (SAGG)x4 linker between GAL4(1-65) and the PC…

   Promoter

   CMVd1

   Available since

   May 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. LSM0254 (PB)EF1a-ADAR2-T2A-PuroR-P2A-BFP

   Plasmid

   #233445

   Purpose

   PiggyBac donor plasmid to overexpress a full length ADAR2

   Depositor

   Xiaojing Gao

   Article

   Zhang et al Nat Chem Biol. 2025 Mar 28. doi: 10.1038

   Insert

   ADAR2-T2A-PuroR-P2A-BFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   WT

   Promoter

   EF1a

   Available since

   April 1, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. AAV phSyn1(S)-FLEX-tdTomato-T2A-SypEGFP-WPRE

   Plasmid

   #51509

   Purpose

   Can be used to generate AAV virus that will express cytoplasmic tdTomato and presynaptic (synaptophysin-fused) EGFP in the presence of Cre in neurons from the synapsin promoter

   Depositor

   Hongkui Zeng

   Article

   Oh et al Nature. 2014 Apr 10;508(7495):207-14. do

   Has Service

   AAV1

   Insert

   tdTomato-T2A-SypEGFP
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   phSyn1

   Available since

   May 2, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLV-TRE3G-hYAP1-CMV-mCherry-T2A-Puro

   Plasmid

   #176850

   Purpose

   lentiviral vector for doxycycline-inducible YAP1 overexpression

   Depositor

   Roland Friedel

   Article

   Junqueira Alves et al Nat Commun. 2021 Oct 14;12(1):6019. doi:

   Insert

   hYAP1 (YAP1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   full length YAP1

   Promoter

   Available since

   Nov. 5, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLV hUbC-VP64 dCas9 VP64-T2A-GFP

   Plasmid

   #59791

   Purpose

   Co-expresses human optimized S. pyogenes dCas9 fused to two copies of VP64 and GFP

   Depositor

   Charles Gersbach

   Article

   Kabadi et al Nucleic Acids Res. 2014 Aug 13. pii: gku

   Insert

   humanized VP64 dead Cas9 VP64 T2A GFP
   

   Use

   CRISPR and Lentiviral

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   D10A and H840A

   Promoter

   Available since

   Oct. 23, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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8. pUC19-OCT4-T2A-NLS-EmGFP-P2A-Puro

   Plasmid

   #89992

   Purpose

   Donor template for generation of OCT4-T2A-nEmGFP reporter cell lines

   Depositor

   Timo Otonkoski

   Article

   Balboa et al Stem Cell Res. 2017 Aug;23:105-108. doi:

   Insert

   OCT4-T2A-NLS-EmGFP-P2A-Puro (POU5F1 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   none

   Available since

   Sept. 22, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. P-Ef1a-RPL22-3XFlag-P2A-EGFP-T2A-Puro

   Plasmid

   #170318

   Purpose

   Expresses FLAG and EGFP tagged RPL22, puromycin resistance

   Depositor

   Hemali Phatnani

   Article

   Gregory et al Cells. 2020 Jun 5;9(6). pii: cells906140

   Insert

   RPL22 (RPL22 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   None

   Promoter

   Available since

   May 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pEx1-pEF-H2B-mCherry-T2A-rTetR-KRAB

    Plasmid

    #78348

    Purpose

    Expresses rTetR-KRAB for gene silencing and the nuclear H2B-mCherry marker in mammalian cells.

    Depositor

    Michael Elowitz

    Article

    Bintu et al Science. 2016 Feb 12;351(6274):720-4. do

    Insert

    H2B-mCherry-T2A-rTetR-KRAB
    

    Use

    Synthetic Biology

    Tags

    rTetR

    Expression

    Mammalian

    Mutation

    Promoter

    pEF alpha

    Available since

    June 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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