We narrowed to 8,363 results for: construct
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Plasmid#107249PurposeVipp1-mGFPmut3 (or vipp1-gfp) construct with spectinomycin resistance cassette downstream used to replace the native copy of Vipp1 in Synechocystis (sll0617).DepositorInsertvipp1-gfp
UseSuicide vector for destined for double homologous…TagsmGFPmut3ExpressionBacterialMutationPromoternative promoter of sll0617Available sinceApril 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
AAV-flex-oChIEF-citrine
Plasmid#50973Purposeflexed oChIEF-citrine construct in an AAV transfer vector containing AAV2 ITRDepositorInsertChIEF-citrine
UseAAVTagscitrineExpressionMutationinverted and inserted between two lox sitesPromoterhuman synapsin promoterAvailable sinceFeb. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
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TetO7-HSP30UTR-nLucPEST-MS2(v4)
Plasmid#199764PurposeControl construct of elongation reporter to quantify elongation of LacZDepositorInsertNanolucPEST
UseTagsExpressionYeastMutationPromoterTetO7Available sinceMay 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-FRT-GFP-mCherry-3pGW
Plasmid#53965PurposeBidirectional construct in the pcDNA5/FRT backbone with GFP and mCherry driven by the same CMV promoter along with a Gateway cloning site downstream mCherryDepositorInsertsGFP
mCherry
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceAug. 22, 2014AvailabilityAcademic Institutions and Nonprofits only -
pQE30del
Plasmid#106902PurposepQE30del is conditionally replicating empty vector. Replication of the plasmid is inhibited in presense of IPTG or lactose. pQE30del can be used for construction of any E. coli helper plasmids.DepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceJune 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLenti-sgRNA-Lib
Plasmid#53121PurposesgRNA expression construction in a 3rd generation lentiviral backboneDepositorTypeEmpty backboneUseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6Available sinceMay 27, 2014AvailabilityAcademic Institutions and Nonprofits only -
pSe-Rp1
Plasmid#111644PurposeFor construction of spacer and repeat arrays for Sulfolobus islandicus REY154ADepositorTypeEmpty backboneUseArchaeal expression vectorTagsExpressionMutationPromoteraraSAvailable sinceJuly 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
Rad51–Cas9(D10A)
Plasmid#125567PurposeExpresses the Rad51–Cas9(D10A) fusion construct in mammalian cells under CMV promoterDepositorInsertRad51–Cas9(D10A) (RAD51 Human)
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceMay 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMM310-Myc
Plasmid#80053PurposeExpression of a Myc tagged version of the BlaM-Vpr fusion proteinDepositorInsertMyc-BlaM-Vpr
UseTagsMycExpressionMammalianMutationN-terminal Myc tag added to construct pMM310PromoterAvailable sinceAug. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
PEP109E
Plasmid#97395Purposebinary construct of cytosolic mCherry with sfGFP11 tag for plant expressionDepositorInsertmCherry-sfGFP11
UseTagscytosolExpressionPlantMutationPromoterAvailable sinceSept. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
EcMBP165-cpGFP.PPYF.T203V.pRSET
Plasmid#33373Purposea single-wavelength maltose sensor constructed from E. coli MalE and cpGFPDepositorInsertImproved MBP based Maltose Biosensor
UseTags6xHISExpressionBacterialMutationcpGFP146 variant from GCaMP2 inserted between AA1…PromoterT7Available sinceDec. 21, 2011AvailabilityAcademic Institutions and Nonprofits only -
pJWV25
Plasmid#61045PurposeTo construct N-terminal GFP fusions under control of a Zn2+ inducible promoter, integrates via double crossover at bgaADepositorTypeEmpty backboneUseSynthetic BiologyTagsGFP+ExpressionBacterialMutationPromoterAvailable sinceJan. 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
pYC56
Plasmid#63910PurposeRecipient integrative vector to construct mCherry tagged fusionsDepositorTypeEmpty backboneUseTagsmCherryExpressionYeastMutationPromoterAvailable sinceApril 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
PEP110E
Plasmid#97396Purposebinary construct of nuclear mCherry with sfGFP11 tag for plant expressionDepositorInsertmCherry-sfGFP11
UseTagsNucleus targetExpressionPlantMutationPromoterAvailable sinceSept. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCB-CBEv2
Plasmid#221139PurposeCoxiella burnetii CRISPR-Cas9 cytosine base editing plasmid version 2 without sgRNA construct. Expresses 3xF-BE4-PpAPOBEC1(H122A).DepositorInsert3xF-PpAPOBEC1(H122A)-Cas9n-UGI-UGI (BE4-PpAPOBEC1(H122A))
UseCRISPRTags3xFLAGExpressionBacterialMutationPromoterlacIq-PtacAvailable sinceNov. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
ER-GCaMP6f-AAV
Plasmid#182501PurposeGFAP-ER-GCaMP6f AAV: An AAV5 constructed for ER-GCaMP6f with astrocyte specific GFAP promotorDepositorInsertER localized P450
UseAAVTagsExpressionMutationPromoterAvailable sinceApril 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
L304-EGFP-Tubulin-K40R
Plasmid#64059PurposeEGFP fused at the N-terminus of tubulin with a mutation K40R; lentiviral constructDepositorInserttubulin
UseLentiviralTagsEGFPExpressionMammalianMutationPromoterAvailable sinceMay 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCB-CBEv1
Plasmid#221138PurposeCoxiella burnetii CRISPR-Cas9 cytosine base editing plasmid version 1 without sgRNA construct. Expresses 3xF-HF-BE3.DepositorInsert3xF-rAPOBEC1-Cas9n(HF)-UGI (HF-BE3)
UseCRISPRTags3xFLAGExpressionBacterialMutationPromoterlacIq-PtacAvailable sinceNov. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pADH147
Plasmid#90991PurposeNAT-marked "empty" gRNA construct for use in gRNA expression cassette stitching PCR. Use with pADH110 to generate target-specific part 2 of 2 for C.alb HIS1-FLP CRISPR system.DepositorInsertgRNA conserved, FRT, DS_HIS1
UseCRISPRTagsExpressionMutationPromoterAvailable sinceJune 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
PEP104E
Plasmid#97390Purposebinary construct of mitochondria-targeted sfGFP1-10 for plant expressionDepositorInsertsfGFP1-10OPT
UseTagsmitochondria targetExpressionPlantMutationPromoterAvailable sinceOct. 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
pADH119
Plasmid#90985PurposeNAT-marked "empty" gRNA construct for use in gRNA expression cassette stitching PCR. Use with pADH110 to generate target-specific part 2 of 2 for C.alb LEUpOUT CRISPR system.DepositorInsertgRNA conserved, C. albicans LEU2 2 of 2
UseCRISPRTagsExpressionMutationPromoterAvailable sinceJune 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
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pTC201B
Plasmid#52193Purposefor creating reversible targeting constructDepositorInsertpromoter-trap cassette Neo-Ires-GFP
UseCre/LoxTagsNeo, GFPExpressionMutationPromoterAvailable sinceApril 23, 2014AvailabilityAcademic Institutions and Nonprofits only -
pBMN-mEGFP-C1
Plasmid#110468Purposeretrovirus construct for stable expressing mEGFP tagged proteinDepositorInsertmEGFP
UseRetroviral; RetrovirusTagsmEGFPExpressionMammalianMutationPromoterAvailable sinceFeb. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
TALED_Right-stuffer-1397N
Plasmid#187172Purposeoriginal vector to make all constructsDepositorInsertRight-stuffer-1397N
UseTagsExpressionMammalianMutationPromoterpCMVAvailable sinceAug. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pKT-mIDH1(R132H)-Myc-FLAG-IRES-Kat
Plasmid#133779PurposeExpresses IDH1 with a R132H mutation and has Myc and FLAG tags. IDH1(R132H) also has a katushka fluorescent reporter. Construct has inverted repeats to be used in Sleeping Beauty transposon system.DepositorInsertIsocitrate dehydrogenase 1 (IDH1 Human)
UseTagsMyc and FLAGExpressionMammalianMutationArginine is mutated to histidine at amino acid po…PromoterAvailable sinceOct. 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET28a-StrepII-TagRFP-CohI
Plasmid#58710PurposeDetection construct to label dockerin type I containing fusion proteinsDepositorInsertTagRFP-CohI
UseTagsStrepIIExpressionBacterialMutationPromoterT7Available sinceAug. 25, 2014AvailabilityAcademic Institutions and Nonprofits only -
PEP112E
Plasmid#97398Purposebinary construct of mitochondria-targeted mCherry with sfGFP11 tag for plant expressionDepositorInsertmCherry-sfGFP11
UseTagsMitochondria targetExpressionPlantMutationPromoterAvailable sinceSept. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
JAB2296
Plasmid#190365PurposePlasmids encode parts for syntetic genetic circuit construction in eudicot plants (proUBQ10::PIP2A-mCherry-NOSt_pro35S-PhlFO::GFPint-NLS-ADHt)DepositorInsertproUBQ10::PIP2A-mCherry-NOSt_pro35S-PhlFO::GFPint-NLS-ADHt
UseTagsExpressionPlantMutationPromoterAvailable sinceMay 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pYC34
Plasmid#63915PurposeRecipient overexpressing vector to construct mCherry tagged fusionsDepositorTypeEmpty backboneUseTagsmCherryExpressionYeastMutationPromoterTEF1 (Saccharomyces cerevisiae)Available sinceApril 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
CMVTO-IL12-26Sfur-B2AD-tvmvs-KKMP
Plasmid#179646PurposeTVMVP-inducible IL-12 RELEASE constructDepositorInsertIL12 A/B
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLKO-perox-tagRFP
Plasmid#185600PurposeLentiviral construct for expression of tagRFP-T targeted to peroxisomesDepositorInsertperox-tagRFP-T
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceAug. 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
PEP111E
Plasmid#97397Purposebinary construct of plastid-targeted mCherry with sfGFP11 tag for plant expressionDepositorInsertmCherry-sfGFP11
UseTagsPlastid targetExpressionPlantMutationPromoterAvailable sinceSept. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAE03
Plasmid#61044PurposeTo construct C-terminal GFP fusions, single crossover integration vectorDepositorTypeEmpty backboneUseSynthetic BiologyTagsGFP+ExpressionBacterialMutationPromoterAvailable sinceFeb. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pJB01
Plasmid#111957PurposeExpresses His10-RFP-Halo; construct generated for pull-down and localization experimentsDepositorInsertRFP
UseTagsHaloTagExpressionBacterialMutationPromoterT7Available sinceAug. 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV hSynapsin flexed SV-tag
Plasmid#163686Purposeexpress SV-Tag construct in a Cre-dependent mannerDepositorInsertsynaptophysin 9X HA
UseAAVTags9X HAExpressionMammalianMutationPromotersynapsinAvailable sinceJan. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBS MCK CRE
Plasmid#12529DepositorInsertCre recombinase
UseCre/Lox; Transgenic constructTagsExpressionMutationCre is driven by the Muscle Creatine Kinase promo…PromoterAvailable sinceAug. 24, 2006AvailabilityAcademic Institutions and Nonprofits only -
JAB3312
Plasmid#190406PurposePlasmids encode parts for syntetic genetic circuit construction in eudicot plants (proUBQ10::PIP2A-mCherry-NOSt_pro35S-AmtRO-PhlFO::GFPint-NLS-ADHt)DepositorInsertproUBQ10::PIP2A-mCherry-NOSt_pro35S-AmtRO-PhlFO::GFPint-NLS-ADHt
UseTagsExpressionPlantMutationPromoterAvailable sinceApril 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
JAB3603
Plasmid#190422PurposePlasmids encode parts for syntetic genetic circuit construction in eudicot plants (proUBQ10::PIP2A-mCherry-NOSt_4XPhlFO::GFPint-ADHt)DepositorInsertproUBQ10::PIP2A-mCherry-NOSt_4XPhlFO::GFPint-ADHt
UseTagsExpressionPlantMutationPromoterAvailable sinceApril 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
JAB2493
Plasmid#190375PurposePlasmids encode parts for syntetic genetic circuit construction in eudicot plants (proUBQ10::PIP2A-mCherry-NOSt_6XLacIO-min35S::GFPint-ADHt)DepositorInsertproUBQ10::PIP2A-mCherry-NOSt_6XLacIO-min35S::GFPint-ADHt
UseTagsExpressionPlantMutationPromoterAvailable sinceApril 4, 2023AvailabilityAcademic Institutions and Nonprofits only -
JAB2192
Plasmid#190356PurposePlasmids encode parts for syntetic genetic circuit construction in eudicot plants (proUBQ10::PIP2A-mCherry-NOSt_6XQacRO-min35S::GFPint-ADHt)DepositorInsertproUBQ10::PIP2A-mCherry-NOSt_6XQacRO-min35S::GFPint-ADHt
UseTagsExpressionPlantMutationPromoterAvailable sinceMarch 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
JAB2191
Plasmid#190355PurposePlasmids encode parts for syntetic genetic circuit construction in eudicot plants (proUBQ10::PIP2A-mCherry-NOSt_6XLmrAO-min35S::GFPint-ADHt)DepositorInsertproUBQ10::PIP2A-mCherry-NOSt_6XLmrAO-min35S::GFPint-ADHt
UseTagsExpressionPlantMutationPromoterAvailable sinceMarch 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
SPDK3181
Plasmid#97403Purposebinary construct of ER-targeted sfCherry1-10 for plant expressionDepositorInsertER-sfCherry1-10
UseTagsER targetExpressionPlantMutationPromoterAvailable sinceSept. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
SPDK3182
Plasmid#97404Purposebinary construct of ER-targeted sfYFP1-10 for plant expressionDepositorInsertER-sfYFP1-10
UseTagsER targetExpressionPlantMutationPromoterAvailable sinceOct. 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
SPDK3184
Plasmid#97405Purposebinary construct of cytosolic sfCFP1-10 tag for plant expressionDepositorInsertsfCFP1-10
UseTagscytosolExpressionPlantMutationPromoterAvailable sinceSept. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
PEP106E
Plasmid#97392Purposebinary construct of ER-targeted sfGFP1-10 for plant expressionDepositorInsertsfGFP1-10OPT
UseTagsER targetExpressionPlantMutationPromoterAvailable sinceSept. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMS17: pTwinStrep-SUMO-AvTnsD
Plasmid#168150PurposeAvTnsD expression construct for protein purification.DepositorInsertAvCAST TnsD
UseTags6X His, Twin Strep and SUMOExpressionBacterialMutationPromoterAvailable sinceJune 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMS15: pTwinStrep-SUMO-AvTnsB
Plasmid#168148PurposeAvTnsB expression construct for protein purification.DepositorInsertAvCAST TnsB
UseTags6X His, Twin Strep and SUMOExpressionBacterialMutationPromoterAvailable sinceJune 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMS16: pTwinStrep-SUMO-AvTnsC
Plasmid#168149PurposeAvTnsC expression construct for protein purification.DepositorInsertAvCAST TnsC
UseTags6X His, Twin Strep and SUMOExpressionBacterialMutationPromoterAvailable sinceJune 2, 2021AvailabilityAcademic Institutions and Nonprofits only