We narrowed to 15,983 results for: LEA

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Tol2_ISCro4_Deletion_Reporter

Plasmid

#251498

Purpose

Reporter plasmid for monitoring deletion activity of ISCro4 bridge recombinase in the presence of native bridge RNA or native TBL and DBL components

Depositor

Martin Jinek

Article

Pelea et al Science, 2026. eadz1884, doi:10.1126/sci

Insert

ISCro4 deletion reporter

Expression

Mammalian

Mutation

NA

Available Since

Feb. 6, 2026

Availability

Academic Institutions and Nonprofits only

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Tol2_ISCro4_Inversion_Reporter

Plasmid

#251499

Purpose

Reporter plasmid for monitoring inversion activity of ISCro4 bridge recombinase in the presence of native bridge RNA or native TBL and DBL components

Depositor

Martin Jinek

Article

Pelea et al Science, 2026. eadz1884, doi:10.1126/sci

Insert

ISCro4 inversion reporter

Expression

Mammalian

Mutation

NA

Available Since

Feb. 5, 2026

Availability

Academic Institutions and Nonprofits only

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Tol2_ISCro4_Insertion_Reporter

Plasmid

#251500

Purpose

Reporter plasmid for monitoring insertion activity of ISCro4 bridge recombinase in the presence of native bridge RNA or native TBL and DBL and pDonor_ISCro4_(WT)_Donor_ECFP plasmid

Depositor

Martin Jinek

Article

Pelea et al Science, 2026. eadz1884, doi:10.1126/sci

Insert

ISCro4 insertion reporter

Expression

Mammalian

Mutation

NA

Available Since

Feb. 5, 2026

Availability

Academic Institutions and Nonprofits only

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pDonor_ISCro4_(WT)_Donor_ECFP

Plasmid

#251501

Purpose

Donor plasmid for monitoring insertion activity of ISCro4 bridge recombinase in the presence of native bridge RNA or native TBL and DBL and Tol2_ISCro4_Insertion_Reporter plasmid

Depositor

Martin Jinek

Article

Pelea et al Science, 2026. eadz1884, doi:10.1126/sci

Insert

ISCro4_(WT)_Donor sequence and ECFP

Expression

Mammalian

Mutation

NA

Available Since

Feb. 5, 2026

Availability

Academic Institutions and Nonprofits only

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U6_ISCro4_(WT)_TBL_bridge_RNA_polIII_term

Plasmid

#251496

Purpose

Expression of the Target Binding Loop (TBL) component of the native ISCro4 bridge RNA

Depositor

Martin Jinek

Article

Pelea et al Science, 2026. eadz1884, doi:10.1126/sci

Insert

ISCro4 bridge RNA Target Binding Loop (TBL)

Expression

Mammalian

Mutation

NA

Available Since

Feb. 5, 2026

Availability

Academic Institutions and Nonprofits only

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U6_ISCro4_(WT)_DBL_bridge_RNA_polIII_term

Plasmid

#251497

Purpose

Expression of the Donor Binding Loop (DBL) component of the native ISCro4 bridge RNA

Depositor

Martin Jinek

Article

Pelea et al Science, 2026. eadz1884, doi:10.1126/sci

Insert

ISCro4 bridge RNA Donor Binding Loop (DBL)

Expression

Mammalian

Mutation

NA

Available Since

Feb. 5, 2026

Availability

Academic Institutions and Nonprofits only

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Split PS Intein gal4-vp16

Plasmid

#242030

Purpose

Blue-light-controlled release of gal4-vp16 transactivator from the cytoplasm to the nucleus. Split version.

Depositor

Vladislav Verkhusha

Article

Baloban et al Nat Commun. 2025 Sep 11;16(1):8263. doi:

Insert

Split PS Intein gal4-vp16

Expression

Mammalian

Mutation

pNL3.2.NF-KB-RE[NlucP/NF-KB-RE/Hygro]

Promoter

CMV

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pCAGEN_NES_V5_GFP_FTA

Plasmid

#235633

Purpose

Encodes V5-GFP with an N-terminal nuclear export sequence and a C-terminal FLAG-TEV cleavage site-AviTag. Used to express a control substrate for ALIBi.

Depositor

Maria Barna

Article

Zhang et al Science. 2025 Mar 7;387(6738):eadn2623.

Insert

GFP

Tags

FLAG-TEV cleavage site-AviTag and NES-V5

Expression

Mammalian

Available Since

April 2, 2025

Availability

Academic Institutions and Nonprofits only

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pCAG-nucGFP11-3xPax7gRNA

Plasmid

#224570

Purpose

Ubiquitous expression plasmid, CAG promoter (CMV immediate early enhancer, chicken beta actin promoter), three unique Pax7 gRNAs with ribozyme self-cleavage sites, and nuclear split GFP(11) reporter.

Depositor

Erica Hutchins

Article

Kim et al Dev Biol. 2024 Nov 30:S0012-1606(24)0026

Insert

GFP11-H2B

Use

CRISPR

Tags

Histone H2B (nuclear localization)

Mutation

Codon optimized

Available Since

Dec. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pPicZ:mTREK-1_cryst_I110D

Plasmid

#209640

Purpose

P. pastoris expression vector. It will generate the mouse TREK-1 channel (21-322, I110D) fused to a C-terminal GFP

Depositor

Dan Minor

Article

Pope et al Cell Chem Biol. 2020 May 21;27(5):511-52

Insert

Potassium channel subfamily K member 2 (KCNK2, TREK-1) I110D mutant

Tags

SNS- 3C_cleavage_site-TAAA-GFP

Expression

Yeast

Mutation

aa 21-322, K84R, Q85E, T86K, I88L, A89R, Q90A, A9…

Available Since

Sept. 10, 2024

Availability

Academic Institutions and Nonprofits only

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Src(YF)-SsrA(Y7I)-mCer3

Plasmid

#220999

Purpose

this is microTag (Y7I mutation in SsrA)-Src(Y529F) with mCerulean3 at C-terminal

Depositor

Klaus Hahn

Article

Liu et al Cell. 2021 Oct 28;184(22):5670-5685.e23.

Insert

Src, SsrA, mCerulean3 (Src Mouse)

Tags

mCerulean3

Expression

Mammalian

Mutation

Y529F in Src, Y7I in SsrA

Promoter

CMV

Available Since

June 12, 2024

Availability

Academic Institutions and Nonprofits only

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Src(KD/YF)-SsrA(Y7I)-mCer3

Plasmid

#221000

Purpose

this is microTag (Y7I mutation in SsrA)-Src(D388R/Y529F) with mCerulean3 at C-terminal

Depositor

Klaus Hahn

Article

Liu et al Cell. 2021 Oct 28;184(22):5670-5685.e23.

Insert

Src, SsrA, mCerulean3 (Src Mouse)

Tags

mCerulean3

Expression

Mammalian

Mutation

D388R/Y529F in Src, Y7I in SsrA

Promoter

pCMV

Available Since

June 12, 2024

Availability

Academic Institutions and Nonprofits only

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pExpreS2-1-CR-PA

Plasmid

#175445

Purpose

HiFi-compatible destination vector for secreted overexpression of thrombin-cleavable, C-terminal Protein A tagged proteins with ExpreS2 Platform

Depositor

Wyatt Yue

Article

Coker et al Front Bioeng Biotechnol. 2022 May 5;10:8

Type

Empty backbone

Tags

BiP Secretion Peptide and Thrombin-Protein A

Expression

Insect

Promoter

Fused Actin-HSP70

Available Since

March 30, 2022

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmNot3_A

Plasmid

#145895

Purpose

Insect Expression of DmNot3

Depositor

Elisa Izaurralde

Article

Zekri et al Mol Cell Biol. 2009 Dec;29(23):6220-31.

Insert

DmNot3 (Not3 Fly)

Expression

Insect

Mutation

two silent mutations compared to the sequence giv…

Available Since

Feb. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pU6-sgRNA EF1Alpha-puro-T2A-BFP.DIS3L2-3

Plasmid

#128764

Purpose

Expresses DIS3L2 gRNA for CRISPRi

Depositor

David Bartel

Article

Kleaveland et al Cell. 2018 Jul 12;174(2):350-362.e17. do

Insert

DIS3L2 sgRNA #3 (DIS3L2 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

mU6

Available Since

Oct. 15, 2019

Availability

Academic Institutions and Nonprofits only

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pU6-sgRNA EF1Alpha-puro-T2A-BFP.DIS3L2-2

Plasmid

#128763

Purpose

Expresses DIS3L2 gRNA for CRISPRi

Depositor

David Bartel

Article

Kleaveland et al Cell. 2018 Jul 12;174(2):350-362.e17. do

Insert

DIS3L2 sgRNA #2 (DIS3L2 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

mU6

Available Since

Oct. 15, 2019

Availability

Academic Institutions and Nonprofits only

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pU6-sgRNA EF1Alpha-puro-T2A-BFP.DIS3L2-1

Plasmid

#128762

Purpose

Expresses DIS3L2 gRNA for CRISPRi

Depositor

David Bartel

Article

Kleaveland et al Cell. 2018 Jul 12;174(2):350-362.e17. do

Insert

DIS3L2 sgRNA #1 (DIS3L2 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

mU6

Available Since

Oct. 15, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA4/TO/FLAG-hPMR1(2H)-Tev-Bio

Plasmid

#80325

Purpose

Expresses a catalytically inactive form of human PMR1 endonuclease with N-terminal FLAG and C-terminal biotinylation tags

Depositor

Daniel Schoenberg

Article

Gu et al RNA. 2012 Jun;18(6):1186-96. doi: 10.126

Insert

PXDNL-003 (PXDNL Human)

Tags

Biotin

Expression

Mammalian

Mutation

H170A and H256A

Promoter

CMV

Available Since

Aug. 19, 2016

Availability

Academic Institutions and Nonprofits only

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NET39 Short pmRFP-N2 (451)

Plasmid

#61981

Purpose

mammalian expression of nuclear envelope transmembrane protein

Depositor

Eric Schirmer

Article

Malik et al Cell Mol Life Sci. 2010 Apr;67(8):1353-6

Insert

NET39 (PPAPDC3 Human)

Tags

mRFP

Expression

Mammalian

Mutation

Short form of NET39

Promoter

CMV

Available Since

Aug. 17, 2015

Availability

Academic Institutions and Nonprofits only
pHL836

Plasmid

#62827

Purpose

Lim Lab plasmid: AmpR + pLlacO-1::ompC::gfp::T1T2 terminator + PLtetO-1::ipex::Asp terminator + PLtetO-1::RBS (st7) hfq::T1 terminator + ColE1

Depositor

Han Lim

Article

Sagawa et al Nucleic Acids Res. 2015 Aug 10. pii: gkv

Inserts

gfp
ipex leader
hfq leader

Use

Synthetic Biology

Tags

ompC leader and st7 RBS

Expression

Bacterial

Promoter

pLlacO-1 and pLtetO-1

Available Since

Aug. 14, 2015

Availability

Academic Institutions and Nonprofits only