We narrowed to 15,732 results for: LEA

1. FASTHDR-Cterm-mTagBFP2-Blasticidin

   Plasmid

   #167207

   Purpose

   Plasmid to clone recombination arms to create homologous recombination donor vector for C-terminal gene tagging with mTagBFP2 and selection with Blasticidin

   Depositor

   Oscar Perez

   Article

   Perez-Leal et al CRISPR J. 2021 Nov 30. doi: 10.1089/cris

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   mTagBFP2

   Expression

   Mammalian

   Available Since

   Dec. 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. SuExp His-Myc-mPLD3

   Plasmid

   #173853

   Purpose

   Protein expression plasmid for recombinant His-Myc tag mouse PLD3

   Depositor

   David Nemazee

   Article

   Gavin et al Nat Commun. 2021 Oct 7;12(1):5874. doi:

   Insert

   PLD3 (Pld3 Mouse)
   

   Tags

   6xHis, Myc, Factor X cleavable

   Expression

   Mammalian

   Mutation

   intracellular and transmembrane domain truncated,…

   Promoter

   CMV

   Available Since

   Jan. 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. Perox-mTurq2-Apollo-NADP+

   Plasmid

   #193304

   Purpose

   Expresses mTurq2-Apollo-NADP+ sensor localized to the peroxisome

   Depositor

   Jonathan Rocheleau

   Article

   Chang et al ACS Sens. 2022 Oct 21. doi: 10.1021/acss

   Insert

   G6PD (G6PD Human)
   

   Tags

   mTurqoise2, peroxisomal targeting sequence

   Expression

   Mammalian

   Mutation

   S40A, R72Q, H201N, K205T

   Promoter

   T7

   Available Since

   Feb. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. Mito-mTurq2-Apollo-NADP+

   Plasmid

   #193303

   Purpose

   Expresses mTurq2-Apollo-NADP+ sensor localized to the mitochondria

   Depositor

   Jonathan Rocheleau

   Article

   Chang et al ACS Sens. 2022 Oct 21. doi: 10.1021/acss

   Insert

   G6PD (G6PD Human)
   

   Tags

   mTurqoise2 and mitochondrial targeting sequence

   Expression

   Mammalian

   Mutation

   S40A, R72Q, H201N, K205T

   Promoter

   T7

   Available Since

   May 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. DHFR-mNeon-GluA1

   Plasmid

   #173009

   Purpose

   For light regulated release of GluA1 from the endoplasmic reticulum. Driven by synapsin promoter. Intracellular trafficking can be better visualized with mNeon.

   Depositor

   Matthew Kennedy

   Article

   Bourke et al J Cell Biol. 2021 Sep 6;220(9). pii: 212

   Insert

   DHFR-mNeon-GluA1
   

   Tags

   DHFR and mNeon

   Expression

   Mammalian

   Promoter

   Chicken beta actin (CAG)

   Available Since

   Aug. 5, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. VZV ORF 60 (gL)

   Plasmid

   #64082

   Purpose

   eukaryotic expression plasmid for VZV glycoprotein L

   Depositor

   Randall Cohrs

   Article

   Birlea et al J Virol. 2013 Jan;87(1):415-21. doi: 10.

   Insert

   VZV glycoprotein L
   

   Expression

   Mammalian

   Promoter

   CMV IE with intron

   Available Since

   Aug. 12, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. VZV ORF 37(gH)

   Plasmid

   #64081

   Purpose

   eukaryotic expression plasmid for VZV glycoprotein H

   Depositor

   Randall Cohrs

   Article

   Birlea et al J Virol. 2013 Jan;87(1):415-21. doi: 10.

   Insert

   VZV glycoprotein H
   

   Expression

   Mammalian

   Promoter

   CMV IE with intron

   Available Since

   Aug. 12, 2015

   Availability

   Academic Institutions and Nonprofits only

8. NKP90 RFP (787)

   Plasmid

   #62001

   Purpose

   mammalian expression of nuclear envelope transmembrane protein

   Depositor

   Eric Schirmer

   Article

   Korfali et al Mol Cell Proteomics. 2010 Dec;9(12):2571

   Insert

   NKP90 (TMEM126A Human)
   

   Tags

   mRFP

   Expression

   Mammalian

   Promoter

   pgk

   Available Since

   Jan. 22, 2015

   Availability

   Academic Institutions and Nonprofits only

9. SuExp His-Myc-mPLD4

   Plasmid

   #201185

   Purpose

   Protein expression plasmid for recombinant His-Myc tag mouse PLD4

   Depositor

   David Nemazee

   Article

   Gavin et al Nat Commun. 2021 Oct 7;12(1):5874. doi:

   Insert

   PLD4 (Pld4, Pld4, Pld4 Mouse)
   

   Tags

   His-Myc, Factor Xa cleavable

   Expression

   Mammalian

   Mutation

   cytosolic and transmembrane domain truncated; add…

   Promoter

   CMV

   Available Since

   July 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pαH-S-RRAR

    Plasmid

    #164569

    Purpose

    SARS-CoV-2 Spike protein with Furin site restored (S-RRAR variant)

    Depositor

    Priyamvada Acharya

    Article

    Gobeil et al Cell Rep. 2021 Jan 12;34(2):108630. doi:

    Insert

    Spike (S-RRAR variant)
    

    Tags

    2X Strep-Tag II, 8X His tag, and HRV3C cleavage s…

    Expression

    Mammalian

    Available Since

    Feb. 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. TfR-moxGFP-DHFR

    Plasmid

    #173012

    Purpose

    For light regulated release of transferrin receptor (TfR) from the endoplasmic reticulum. Driven by CMV promoter. Fused to oxidation resistant version of GFP (moxGFP).

    Depositor

    Matthew Kennedy

    Article

    Bourke et al J Cell Biol. 2021 Sep 6;220(9). pii: 212

    Insert

    TfR-moxGFP-DHFR
    

    Tags

    DHFR and moxGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pHS0781 pcDNA3.1(+) CMV-SV40 NLS-AwaIscB-nucleoplasmin NLS-3xHA

    Plasmid

    #176538

    Purpose

    Mammalian expression of nuclear localized AwaIscB

    Depositor

    Feng Zhang

    Article

    Altae-Tran et al Science. 2021 Oct;374(6563):57-65. doi:

    Insert

    AwaIscB
    

    Tags

    Nucleoplasmin NLS-3xHA and SV40 NLS

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pTE2690

    Plasmid

    #137717

    Purpose

    Expresses dimpLbCas12a-SunTag in mammalian cells.

    Depositor

    Ervin Welker

    Article

    Toth et al Nucleic Acids Res. 2020 Feb 28. pii: 576

    Insert

    dimpLbCas12a
    

    Use

    CRISPR

    Tags

    NLSx3 SV40 and SunTagx10

    Expression

    Mammalian

    Mutation

    D156R, G532R, K538V, Y542R, K595R, D832A,

    Promoter

    CMV

    Available Since

    March 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLex_Cas9

    Plasmid

    #117987

    Purpose

    pLex_Cas9 is a single insert lentiviral vector expressing Cas9 driven by CMV promoter.

    Depositor

    Paul Khavari

    Article

    Rubin et al Cell. 2018 Dec 11. pii: S0092-8674(18)31

    Insert

    Cas9-P2A-NLS-BASTR
    

    Use

    Lentiviral

    Tags

    P2A linker, nuclear localization signal and blast…

    Available Since

    Feb. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. Nuc-mTurq2-Apollo-NADP+

    Plasmid

    #193302

    Purpose

    Expresses mTurq2-Apollo-NADP+ sensor localized to the nucleus

    Depositor

    Jonathan Rocheleau

    Article

    Chang et al ACS Sens. 2022 Oct 21. doi: 10.1021/acss

    Insert

    G6PD (G6PD Human)
    

    Tags

    SV40 NLS and mTurqoise2

    Expression

    Mammalian

    Mutation

    S40A, R72Q, H201N, K205T

    Promoter

    T7

    Available Since

    Dec. 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCR2.1_HBB_N

    Plasmid

    #87847

    Purpose

    pCR2.1-based plasmid holding a 309-bp fragment containing the exon-1-exon-2 splice border of normal human β-globin mRNA

    Depositor

    Marina Kleanthous

    Article

    Patsali et al Int J Mol Sci. 2020 Sep 11;21(18). pii:

    Insert

    Homo sapiens hemoglobin subunit beta (HBB), Normal cDNA fragment (Exon1/2) (HBB Human)
    

    Expression

    Bacterial

    Available Since

    Oct. 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA3-HA-TRAIL-R4

    Plasmid

    #61385

    Purpose

    Expresses HA tagged TRAIL receptor 4

    Depositor

    Francis Chan

    Article

    Clancy et al Proc Natl Acad Sci U S A. 2005 Dec 13;10

    Insert

    TRAIL receptor 4 (TNFRSF10D Human)
    

    Tags

    HA and TNF receptor leader peptide

    Expression

    Mammalian

    Mutation

    TRAIL-R4 leader peptide (aa1-29) replaced with TN…

    Available Since

    Jan. 27, 2015

    Availability

    Academic Institutions and Nonprofits only

18. pTE4254

    Plasmid

    #89050

    Purpose

    Expresses St chimeric gRNA and human codon-optimized StCas9

    Depositor

    Ervin Welker

    Article

    Toth et al Biol Direct. 2016 Sep 14;11:46. doi: 10.

    Inserts

    St chimeric gRNA
    hStCas9
    

    Use

    CRISPR

    Tags

    SV40 NLS

    Expression

    Mammalian

    Promoter

    CMV and human U6

    Available Since

    April 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pTE3646

    Plasmid

    #137714

    Purpose

    Expresses LbCas12a RVRR variant in mammalian cells.

    Depositor

    Ervin Welker

    Article

    Toth et al Nucleic Acids Res. 2020 Feb 28. pii: 576

    Insert

    LbCas12a-RVRR
    

    Tags

    3xHA, NLS, and SV40 NLS

    Expression

    Mammalian

    Mutation

    G532R, K538V, Y542R, K595R

    Promoter

    CMV

    Available Since

    March 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA3-AMPKsub(TA)-YPet-NES

    Plasmid

    #84636

    Purpose

    Encodes negative-control C-terminal (substrate) fragment of bimolecular AMPK/BRSK activity reporter (bimABKAR); cytosol targeted; use in conjunction with pcDNA3-Cerulean-FHA1-NES

    Depositor

    Jin Zhang

    Article

    Depry et al Chem Biol. 2015 Nov 19;22(11):1470-9. do

    Insert

    AMPKsub(TA)-YPet-NES
    

    Tags

    6xHis, Nuclear export signal (NES), T7 tag (gene …

    Expression

    Mammalian

    Mutation

    Target Thr residue in substrate domain mutated to…

    Promoter

    CMV

    Available Since

    April 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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