We narrowed to 2,356 results for: pcas9

1. pX330-sgR26

   Plasmid

   #127376

   Purpose

   Used for contransfection with pR26- and pR26-CMVconst-derived constructs to mediate CRISPR/Cas9 genomic insertion into the murine ROSA26 safe harbor locus.

   Depositor

   Lance Miller

   Article

   Routh et al Front Immunol. 2020 Feb 13;11:57. doi: 1

   Insert

   ROSA26 (Gt(ROSA)26Sor Mouse)
   

   Use

   CRISPR and Mouse Targeting

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 13, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. EF1a-TET1-dCas9

   Plasmid

   #235593

   Purpose

   Tet1CD fused to N-terminus of dCas9

   Depositor

   Ting Zhou

   Article

   Wu et al Stem Cell Reports. 2025 Apr 25:102499. d

   Insert

   TET1-dCas9 (TET1 SpCas9 is from Streptococcus pyogenes, Human)
   

   Use

   CRISPR

   Tags

   3xNLS, TET1 CD, and tagBFP

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   May 8, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. Polymerase expression - pCMV-T7-EcKlenow (LM2692)

   Plasmid

   #208963

   Purpose

   Unfused EcKlenow DNA polymerase (-exo), expressed from CMV or T7 promoters.

   Depositor

   Benjamin Kleinstiver

   Article

   Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

   Insert

   EcKlenow-BPNLS
   

   Use

   CRISPR; In vitro transcription; t7 promoter

   Tags

   BPNLS

   Expression

   Mammalian

   Mutation

   EcKlenow(-exo;D355A/E357A)

   Promoter

   CMV and T7

   Available since

   Nov. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pX330-PITCh-ACTB

   Plasmid

   #207748

   Purpose

   Expresses SpCas9, the PITCh gRNA, and a sgRNA targeting the N-terminus of ACTB for knock-in.

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Insert

   sgRNA Targeting N-terminus of ACTB (ACTB Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Nov. 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pABE8e-NG-403Q-Nterm-AAV

   Plasmid

   #194651

   Purpose

   N-terminal AAV genome plasmid encoding ABE8e + sgRNA to correct the R403Q mutation in mice

   Depositor

   David Liu

   Article

   Reichart et al Nat Med. 2023 Feb;29(2):412-421. doi: 10

   Insert

   ABE8e-NG N-term
   

   Use

   AAV

   Tags

   BPNLS and NpuN spilt intein

   Expression

   Mutation

   Promoter

   TNNT2

   Available since

   Feb. 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pABE8e-NG-403Q-Cterm-AAV

   Plasmid

   #194652

   Purpose

   C-terminal AAV genome plasmid encoding ABE8e + sgRNA to correct the R403Q mutation in mice

   Depositor

   David Liu

   Article

   Reichart et al Nat Med. 2023 Feb;29(2):412-421. doi: 10

   Insert

   ABE8e-NG C-term
   

   Use

   AAV

   Tags

   BPNLS and NpuC split intein

   Expression

   Mutation

   Promoter

   TNNT2

   Available since

   Feb. 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pGES401

   Plasmid

   #190198

   Purpose

   For CRISPR-Cas9 mediated multiple gene editing in soybean, single transcript unit system driven by soybean elongation factor 1A promoter (pM4), Basta selection

   Depositor

   Yuefeng Guan

   Article

   Bai et al Mol Plant. 2022 Jul 4;15(7):1081-1083. d

   Insert

   spCas9
   

   Use

   CRISPR

   Tags

   3xFlag

   Expression

   Plant

   Mutation

   plant-codon optimized

   Promoter

   Glycine max elongation factor 1A(pM4)

   Available since

   Sept. 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. p2T-U6-sgGAA

   Plasmid

   #232724

   Purpose

   Cas9 guide RNA targeting GAA repeats for A-to-G base editing

   Depositor

   David Liu

   Article

   Matuszek et al Nat Genet. 2025 May 26. doi: 10.1038/s41

   Insert

   SpCas9 gRNA targeting GAA repeats
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 3, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. CGBE1-NG (pBM1246)

   Plasmid

   #140256

   Purpose

   CMV promoter expression plasmid for UNG(E.coli)-rAPOBEC1(R33A)-nCas9_NG-P2A-EGFP

   Depositor

   Keith Joung

   Article

   Kurt et al Nat Biotechnol. 2020 Jul 20. pii: 10.103

   Insert

   eUNG-BE4max(R33A)_NG-ΔUGI
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   R33A in rAPOBEC1, NG mutations in SpCas9, D10A in…

   Promoter

   CMV

   Available since

   Aug. 5, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. SGyA

    Plasmid

    #160292

    Purpose

    Drives expression of Cas9 and a TdTomato fluorescent marker

    Depositor

    Omar Akbari

    Article

    Gamez et al Nat Commun. 2021 Dec 10;12(1):7202. doi:

    Inserts

    Vas promoter
    hSpCas9
    tdTomato
    CTCF insulator
    Gypsy insulator
    Left homology arm Y chromosome
    Right homology arm Y chromosome
    

    Use

    Tags

    T2A GFP

    Expression

    Insect

    Mutation

    Promoter

    Available since

    March 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. ABE7.10-F148A

    Plasmid

    #132946

    Purpose

    Gene editing. See Gene/Insert section of plasmid page for targeting sequence cloned into this plasmid.

    Depositor

    Hui Yang

    Article

    Zhou et al Nature. 2019 Jul;571(7764):275-278. doi:

    Insert

    ABE7.10(F148A)
    

    Use

    CRISPR; Base editor

    Tags

    Expression

    Mammalian

    Mutation

    ABE7.10(F148A)

    Promoter

    Available since

    Dec. 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. TadA-NG-CBE

    Plasmid

    #227420

    Purpose

    C-to-T base editing in mammalian cells

    Depositor

    Yang Sun

    Article

    Liu et al Adv Sci (Weinh). 2024 Sep 19:e2405628. d

    Insert

    TadA-nSpCas9-NG-2xUGI (AICDA Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pX330-PITCh-COX8A

    Plasmid

    #227308

    Purpose

    Expresses SpCas9, the PITCh gRNA, and a sgRNA targeting the C-terminus of COX8A for knock-in.

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Insert

    sgRNA Targeting C-terminus of COX8A (COX8A Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U67

    Available since

    Nov. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pX330-PITCh-PEX3

    Plasmid

    #227303

    Purpose

    Expresses SpCas9, the PITCh gRNA, and a sgRNA targeting the C-terminus of PEX3 for knock-in.

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Insert

    sgRNA Targeting C-terminus of PEX3 (PEX3 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Nov. 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. Px461-Cas9n-Trp53-sgRNA-beta

    Plasmid

    #88847

    Purpose

    CRISPR KO of Trp53

    Depositor

    Joan Massague

    Article

    Wang et al Cell Stem Cell. 2017 Jan 5;20(1):70-86.

    Insert

    Trp53 (Trp53 Mouse)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 11, 2017

    Availability

    Academic Institutions and Nonprofits only

16. Px461-Cas9n-Trp53-sgRNA-alpha

    Plasmid

    #88846

    Purpose

    CRISPR KO of Trp53

    Depositor

    Joan Massague

    Article

    Wang et al Cell Stem Cell. 2017 Jan 5;20(1):70-86.

    Insert

    Trp53 (Trp53 Mouse)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 11, 2017

    Availability

    Academic Institutions and Nonprofits only

17. pRS0045

    Plasmid

    #131124

    Purpose

    Mammalian Target-ACE expression vector

    Depositor

    Nozomu Yachie

    Article

    Sakata et al Nat Biotechnol. 2020 Jun 1. pii: 10.1038

    Insert

    TadA-linker(32aa)-TadA*(7.10)-linker(32aa)-nCas9-PmCDA1-UGI
    

    Use

    CRISPR

    Tags

    PmCDA1, TadA, and UGI

    Expression

    Mammalian

    Mutation

    A262T, R324L, S409I, E480K, E543D, M694I, E1219V …

    Promoter

    CMV promoter

    Available since

    Sept. 23, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. miniCGBE1-VRQR (pBM1173)

    Plasmid

    #140255

    Purpose

    CMV promoter expression plasmid for rAPOBEC1(R33A)-nCas9_VRQR-P2A-EGFP

    Depositor

    Keith Joung

    Article

    Kurt et al Nat Biotechnol. 2020 Jul 20. pii: 10.103

    Insert

    BE4max(R33A)_VRQR-ΔUGI
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    R33A in rAPOBEC1, VRQR mutations in SpCas9, D10A …

    Promoter

    CMV

    Available since

    Aug. 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. PB-PE

    Plasmid

    #162796

    Purpose

    All-in-one prime editor piggyBac transposon

    Depositor

    Tobias Cantz

    Article

    Eggenschwiler et al Sci Rep. 2021 Nov 12;11(1):22154. doi: 1

    Insert

    SpCas9_ H840A-PE2-MMLV-RT(dBB)-P2A-PAC_dTK(dBB)
    

    Use

    CRISPR and Synthetic Biology; Piggybac transposon

    Tags

    SV40 NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Nov. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCMV_T7-PE2-Nuclease

    Plasmid

    #171997

    Purpose

    Sequence provided for PE-Nuclease mRNA transcription, suitable for microinjection

    Depositor

    Paul Thomas

    Article

    Adikusuma et al Nucleic Acids Res. 2021 Sep 17. pii: 637

    Insert

    CMV_T7-Cas9-RT
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    GC to CA point mutation in SpCas9 to restore nucl…

    Promoter

    CMV for Cas9, U6 for gRNAs

    Available since

    July 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge