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We narrowed to 4,936 results for: AAT

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Showing: 401 - 440 of 4936 results

  1. pLCHKO_SFRS7_exon_deletion_4

    Plasmid
    #155072
    Purpose
    Lentiviral plasmid for expressing U6 driven hybrid guide (hg)RNA for deletion of SFRS7 exon using SpCas9 and LbCas12a nucleases (CHyMErA system)
    Depositor
    Benjamin Blencowe, Jason Moffat
    Article
    Gonatopoulos-Pournatzis et al Nat Biotechnol. 2020 May;38(5):638-648.
    Insert
    (hg)RNA for deletion of SFRS7 exon using SpCas9 and LbCas12a nucleases
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Promoter
    U6
    Available Since
    Aug. 12, 2020
    Availability
    Academic Institutions and Nonprofits only
    155072-plasmid-map-sequence-id-308512

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  5. pLCHKOv3-CD46 Ex3_3-Lb

    Plasmid
    #209029
    Purpose
    Lentiviral vector expressing U6 driven hybrid guide (hgRNA) targeting human CD46 Exon 3 for deletion using SpCas9 and LbCas12a nucleases (CHyMErA system)
    Depositor
    Thomas Gonatopoulos-Pournatzis
    Article
    Xiao et al Mol Cell. 2024 Jul 11;84(13):2553-2572.e
    Insert
    hgRNA for deletion of CD46 Exon 3 with SpCas9 tracrRNAv1 & LbCas12a DR
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Promoter
    U6
    Available Since
    June 20, 2024
    Availability
    Academic Institutions and Nonprofits only
    209029-plasmid-map-sequence-id-414782

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  6. pLCHKOv3-CD46 Ex3_3-As

    Plasmid
    #209033
    Purpose
    Lentiviral vector expressing U6 driven hybrid guide (hgRNA) targeting human CD46 Exon 3 for deletion using SpCas9 and AsCas12a nucleases (CHyMErA system)
    Depositor
    Thomas Gonatopoulos-Pournatzis
    Article
    Xiao et al Mol Cell. 2024 Jul 11;84(13):2553-2572.e
    Insert
    hgRNA for deletion of CD46 Exon 3 with SpCas9 tracrRNAv1 & AsCas12a DR
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Promoter
    U6
    Available Since
    June 20, 2024
    Availability
    Academic Institutions and Nonprofits only
    209033-plasmid-map-sequence-id-413572

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  13. pCAS

    Plasmid
    #60847
    Purpose
    Expresses S. pyogenes Cas9 plus an HDV ribozyme-sgRNA for genome editing in yeast
    Depositor
    Jamie Cate
    Article
    Ryan et al Elife. 2014 Aug 19;3. doi: 10.7554/eLife
    Inserts
    S. pyogenese Cas9
    RNA pol III promoter (tRNA-Tyr)
    hepatitis delta virus ribozyme, genomic
    sgRNA
    Use
    CRISPR and Synthetic Biology
    Tags
    NLS/His8 and TTT 3' extension prior to sgRNA
    Expression
    Bacterial and Yeast
    Mutation
    L4 is UUCG tetraloop and guide targets LYP1 (CATA…
    Available Since
    Jan. 13, 2015
    Availability
    Academic Institutions and Nonprofits only
    60847-plasmid-map-sequence-id-197511

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  28. hNTa1-qgRNA-pYJA5

    Plasmid
    #217779
    Purpose
    Non-targeting control 1 qgRNA-pYJA5 plasmid from the T.gonfio library; it can be used as a ready-to-go control and provides the three constant regions for the three-fragment PCRs for qgRNA cloning
    Depositor
    Adriano Aguzzi
    Article
    Yin et al Nat Biomed Eng. 2024 Dec 4. doi: 10.1038
    Insert
    Quadruple sgRNA-expression cassette for nontargeting control for gene activation and epigenetic silencing
    Use
    CRISPR, Lentiviral, and Synthetic Biology
    Expression
    Mammalian
    Promoter
    human U6, mouse U6, human H1, human 7SK
    Available Since
    March 20, 2025
    Availability
    Academic Institutions and Nonprofits only
    217779-plasmid-map-sequence-id-466021

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  31. pLY70

    Plasmid
    #130948
    Purpose
    A CRISPR activation device with the necessary genes (dxcas9 controlled by Ptet, pspFΔHTH::λN22plus controlled by promoter PrhaB), and the reporter part (PpspA-LEA3B3 with sfgfp::ASV).
    Depositor
    Baojun Wang
    Article
    Liu et al Nat Commun. 2019 Aug 26;10(1):3693. doi:
    Inserts
    dxcas9 (3.7)
    tetR
    rhaS
    pspFΔHTH::λN22plus
    sfgfp
    Use
    Synthetic Biology
    Tags
    ASV tag and pspFΔHTH::λN22plus
    Expression
    Bacterial
    Mutation
    Mutations D10A and H840A on WT cas9 for dCas9; Sy…
    Promoter
    Pcon, PpspA-LEA3B3, PrhaB, and Ptet
    Available Since
    Sept. 23, 2019
    Availability
    Academic Institutions and Nonprofits only
    130948-plasmid-map-sequence-id-257361

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  36. SGEP-shRLuc-308

    Plasmid
    #188667
    Purpose
    control shRNA
    Depositor
    Lewis Cantley
    Insert
    shRLuc
    Use
    CRISPR and Lentiviral
    Mutation
    WT
    Available Since
    Oct. 20, 2022
    Availability
    Academic Institutions and Nonprofits only
    188667-plasmid-map-sequence-id-375067

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  37. pOSR1.1.0-gDNA

    Plasmid
    #176381
    Purpose
    Expresses spCas9 and gRNA sequence targeting OSR1
    Depositor
    Barbara Stranger, Kevin White
    Insert
    OSR1 gRNA
    Use
    CRISPR
    Available Since
    Nov. 19, 2021
    Availability
    Academic Institutions and Nonprofits only
    176381-plasmid-map-sequence-id-347393

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  38. pZBED9.1.2-gDNA

    Plasmid
    #175853
    Purpose
    CRISPR/Cas9 plasmid to create GFP fusion proteins
    Depositor
    Barbara Stranger, Kevin White
    Insert
    ZBED9-Nickase2
    Use
    CRISPR
    Available Since
    Dec. 13, 2021
    Availability
    Academic Institutions and Nonprofits only
    175853-plasmid-map-sequence-id-346180

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  39. pLKO-puro-hPPM1A

    Plasmid
    #185382
    Purpose
    For mammalian expression of shRNA: AGGGTAATGGGTTGCGATATG that targets human PPM1A
    Depositor
    Ayaz Najafov
    Insert
    PPM1A (PPM1A Human)
    Expression
    Mammalian
    Mutation
    WT
    Available Since
    June 8, 2022
    Availability
    Academic Institutions and Nonprofits only
    185382-plasmid-map-sequence-id-363721

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  40. LT3GEPIR-PSMB5shRNA

    Plasmid
    #220580
    Purpose
    To inducibly knockdown PSMB5 expression
    Depositor
    Sean Lee
    Insert
    PSMB5 shRNA (PSMB5 Human)
    Use
    Lentiviral
    Promoter
    TRE3G (TetOP) promoter
    Available Since
    July 1, 2024
    Availability
    Academic Institutions and Nonprofits only
    220580-plasmid-map-sequence-id-437733

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Showing: 401 - 440 of 4936 results