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Plasmid#71701PurposeProduces E. coli thioredoxin reductase (EcTrxB)DepositorInsertthioredoxin reductase
UseTagsExpressionBacterialMutationPromoterT7Available sinceJan. 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
pmr101A-cPr-mNeonGreen (KmR)
Plasmid#177209PurposePlasmid with p15A origin for constitutive mNeonGreen expression in E. coliDepositorInsertmNeonGreen
UseTagsExpressionBacterialMutationYeast codon optimizedPromoterSynthetic constitutive promoterAvailable sinceNov. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJK690
Plasmid#72380PurposeProduces Escherichia coli monothiol glutaredoxin GrxD, N-terminal His6 tag (H6EcGrxD)DepositorInsertglutaredoxin-4
UseTagsHis6ExpressionBacterialMutationPromoterT7Available sinceFeb. 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
Ec holD-pET(3c)
Plasmid#237236Purposeover-express E. coli holD (Pol III psi subunit)DepositorInsertholD (psi) (holD E. coli)
UseTagsExpressionBacterialMutationPromoterAvailable sinceApril 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
Ec dnaN-pET(11a)
Plasmid#237238Purposeover-express E. coli dnaN (Pol III beta sliding clamp subunit)DepositorInsertdnaN (beta) (dnaN E. coli)
UseTagsExpressionBacterialMutationPromoterAvailable sinceApril 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
Ec holA-pET(11a)(ACH-)
Plasmid#237233Purposeover-express E. coli holA (Pol III delta subunit)DepositorInsertholA (delta) (holA E. coli)
UseTagsExpressionBacterialMutationPromoterAvailable sinceApril 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
Ec holC-pET(3c)
Plasmid#237235Purposeover-express E. coli holC (Pol III chi subunit)DepositorInsertholC (chi) (holC E. coli)
UseTagsExpressionBacterialMutationPromoterAvailable sinceApril 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAIP4_1HKA
Plasmid#234169PurposeHeterologous protein expression of 2-amino-4-hydroxy-6-hydroxymethyldihydropteridine pyrophosphokinase in Escherichia coliDepositorInsert1HKA (folK Escherichia coli)
UseTags10x HisTag-Smt3ExpressionBacterialMutationPromoterT7Available sinceMarch 6, 2025AvailabilityIndustry, Academic Institutions, and Nonprofits -
p416-GPD-HA-PPK(mutant)
Plasmid#183944PurposeExpresses HA-tagged catalytic mutant of E. coli PPK from yeast GPD promoterDepositorInsertPPK(mutant) (ppk E. coli)
UseTagsHAExpressionYeastMutationmutations in His435, His454, and His592 to AlaninePromoterGPDAvailable sinceJune 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pmr101A-cPr-mNeonGreen (ErmR)
Plasmid#177208PurposePlasmid with p15A origin for constitutive mNeonGreen expression in E. coliDepositorInsertmNeonGreen
UseTagsExpressionBacterialMutationYeast codon optimizedPromoterSynthetic constitutive promoterAvailable sinceMarch 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCASCADE-udhA-zwf
Plasmid#87153PurposeudhA-zwf targeting guide RNA E. coli , Low Phosphate InductionDepositorInsertudhA-zwf guide array
UseTagsExpressionBacterialMutationPromoterE . coli ugpB gene promoter, low phosphate induct…Available sinceMarch 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
pJK614
Plasmid#72387PurposeProduces Escherichia coli glycinamide ribonucleotide synthetase (EcPurDH6)DepositorInsertphosphoribosylamine-glycine ligase
UseTagsHis6ExpressionBacterialMutationcontains 4 silent mutations in codons 9-11 and 42…PromoterT7Available sinceFeb. 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJK621
Plasmid#72388PurposeProduces Escherichia coli glycinamide ribonucleotide synthetase (EcPurDH6)DepositorInsertphosphoribosylamine-glycine ligase
UseTagsHis6ExpressionBacterialMutationcontains 3 silent mutations in codons 9-11 to fac…PromoterT7Available sinceFeb. 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJK592
Plasmid#72386PurposeProduces Escherichia coli glycinamide ribonucleotide synthetase (EcPurD)DepositorInsertphosphoribosylamine-glycine ligase
UseTagsExpressionBacterialMutationcontains 3 silent mutations in codons 9-11 to fac…PromoterT7Available sinceFeb. 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
pHL1948
Plasmid#53046PurposePconNoHindM12:glgC::gfp, PconNoHind:RBS(st3):tetR, PconNoHindM10:RBS(st3):csrADepositorInsertsGFP
tetR
csrA
UseSynthetic BiologyTagsRBS(st3) and glgC leaderExpressionBacterialMutationPromoterpConNoHind, pConNoHindM10, and pConNoHindM12Available sinceAug. 21, 2014AvailabilityAcademic Institutions and Nonprofits only -
pHL1722
Plasmid#53030PurposePLtetO-1:glgClead::mCherry, PLlacO-1:RBS(st7):csrADepositorInsertsmCherry
csrA
UseSynthetic BiologyTagsRBS(st7) and glgC leaderExpressionBacterialMutationPromoterpLlacO-1 and pLtetO-1Available sinceMay 14, 2014AvailabilityAcademic Institutions and Nonprofits only -
pHL1947
Plasmid#53045PurposePconNoHindM12:glgC::gfp, PconNoHind:RBS(st3):tetR, PconNoHindM8:RBS(st3):csrADepositorInsertsGFP
tetR
csrA
UseSynthetic BiologyTagsRBS(st3) and glgC leaderExpressionBacterialMutationPromoterpConNoHind, pConNoHindM12, and pConNoHindM8Available sinceMay 13, 2014AvailabilityAcademic Institutions and Nonprofits only -
pEcKdtA
Plasmid#36268DepositorInsertE. coli KdtA
UseTagsExpressionMutationPromoteraraCAvailable sinceJuly 24, 2012AvailabilityAcademic Institutions and Nonprofits only -
pSIJ8
Plasmid#68122PurposeTemperature sensitive plasmid for either lambda Red recombinase genes or flippase recombinase expressionDepositorInsertsflp recombinase
rhaS
rhaR
UseTagsExpressionBacterialMutationPromoterrhamnoseAvailable sinceDec. 8, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
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pCS6
Plasmid#55752PurposeProduces T7 RNA Polymerase.DepositorInsertT7 RNA Polymerase
UseTagsExpressionBacterialMutationPromoterpBADAvailable sinceJuly 15, 2014AvailabilityAcademic Institutions and Nonprofits only -
pACYC-Skp-GroEL/ES
Plasmid#83922PurposeCo-expresses cytoplasmic copies of the Skp and Gro EL/ES protein folding chaperones.DepositorInsertsSkp
Gro EL/ES
UseTagsExpressionBacterialMutationPromoterT7Available sinceNov. 28, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCSCX
Plasmid#63918PurposeExpression of the sucrose utilization phenotypeDepositorInsertcscAKB
UseBacterial cloning vectorTagsExpressionMutationPromoterAvailable sinceMay 19, 2015AvailabilityAcademic Institutions and Nonprofits only -
pACYC-Skp-TF
Plasmid#83921PurposeCo-expresses cytoplasmic copies of the Skp and Trigger Factor protein folding chaperones.DepositorInsertsSkp
Trigger Factor
UseTagsExpressionBacterialMutationPromoterT7Available sinceNov. 28, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGEMABC
Plasmid#45398DepositorUseTagsExpressionBacterialMutationPromoterNone and T7Available sinceJune 11, 2013AvailabilityAcademic Institutions and Nonprofits only -
Cra-GFP
Plasmid#220151PurposeTo track the natural Cra promoter's transcriptional dynamicsDepositorInsertCra promoter only
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
CRP-GFP
Plasmid#220152PurposeTo track the natural CRP promoter's transcriptional dynamicsDepositorInsertCRP promoter only
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
Fis-GFP
Plasmid#220153PurposeTo track the natural Fis promoter's transcriptional dynamicsDepositorInsertFis promoter only
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
Lrp-GFP
Plasmid#220158PurposeTo track the natural Lrp promoter's transcriptional dynamicsDepositorInsertLrp promoter only
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
MarA-GFP
Plasmid#220159PurposeTo track the natural MarA promoter's transcriptional dynamicsDepositorInsertMarA promoter only
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
NsrR-GFP
Plasmid#220162PurposeTo track the natural NsrR promoter's transcriptional dynamicsDepositorInsertNsrR promoter only
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
SoxS-GFP
Plasmid#220165PurposeTo track the natural SoxS promoter's transcriptional dynamicsDepositorInsertSoxS promoter only
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
LexA-GFP
Plasmid#220157PurposeTo track the natural LexA promoter's transcriptional dynamicsDepositorInsertLexA promoter only
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
Fur-GFP
Plasmid#220156PurposeTo track the natural Fur promoter's transcriptional dynamicsDepositorInsertFur promoter only
UseTagsExpressionBacterialMutationPromoterAvailable sinceJuly 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
PhoB-GFP
Plasmid#220163PurposeTo track the natural PhoB promoter's transcriptional dynamicsDepositorInsertPhoB promoter only
UseTagsExpressionBacterialMutationPromoterAvailable sinceJuly 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
FlhD-GFP
Plasmid#220154PurposeTo track the natural FlhD promoter's transcriptional dynamicsDepositorInsertFlhD promoter only
UseTagsExpressionBacterialMutationPromoterAvailable sinceJune 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
Fnr-GFP
Plasmid#220155PurposeTo track the natural Fnr promoter's transcriptional dynamicsDepositorInsertFnr promoter only
UseTagsExpressionBacterialMutationPromoterAvailable sinceJune 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
PhoP-GFP
Plasmid#220164PurposeTo track the natural PhoP promoter's transcriptional dynamicsDepositorInsertPhoP promoter only
UseTagsExpressionBacterialMutationPromoterAvailable sinceJune 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
NarL-GFP
Plasmid#220160PurposeTo track the natural NarL promoter's transcriptional dynamicsDepositorInsertNarL promoter only
UseTagsExpressionBacterialMutationPromoterAvailable sinceJune 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDWJ27
Plasmid#207604PurposeGFP-BIB expressionDepositorInsertGFP-BIB
UseTagsExpressionBacterialMutationPromoterAvailable sinceNov. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pJS96
Plasmid#207588PurposeGFP-TonBH20A expressionDepositorInsertGFP-TonBH20A
UseTagsExpressionBacterialMutationH20APromoterAvailable sinceNov. 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
pJS65
Plasmid#207587PurposeGFP-TonB expressionDepositorInsertGFP-TonB
UseTagsExpressionBacterialMutationPromoterAvailable sinceNov. 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGL001
Plasmid#115674PurposeFluorescent reporter for E.coli K12 fliC promoter activityDepositorInsertfliC promoter fused with GFP
UseTagsGreen fluorescent protein (GFP)ExpressionBacterialMutationPromoterfliC promoterAvailable sinceFeb. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMCSG7(gfcC)
Plasmid#176197PurposegfcC protein expressionDepositorInsertgfcC
UseTags6xHis-TeVExpressionBacterialMutationPromoterT7Available sinceOct. 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJL017
Plasmid#53532PurposeThis plasmid encodes both the etpB and etpA two partner secretion genes on an arabinose-inducible plasmid with myc and 6His tags in-frame with the etpA geneDepositorInsertetpBA
UseTags6His and mycExpressionBacterialMutationPromoterpBADAvailable sinceJune 10, 2014AvailabilityAcademic Institutions and Nonprofits only -
pEcCas6
Plasmid#170091Purposeexpresses E. coli type I-E Cas6DepositorInsertE. coli type I-E Cas6
UseTagsHisExpressionBacterialMutationPromoterT7Available sinceJan. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJS61
Plasmid#207585PurposeGFP-TolA expressionDepositorInsertGFP-TolA
UseTagsExpressionBacterialMutationPromoterAvailable sinceNov. 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMCSG7(gfcB)
Plasmid#176196PurposegfcB protein expressionDepositorInsertgfcB
UseTags6xHis-TeVExpressionBacterialMutationPromoterT7Available sinceOct. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
EcYajL pET15b
Plasmid#60716PurposeRecombinant protein expression of YajLDepositorInsertYajL
UseTags6xHisExpressionBacterialMutationPromoterT7Available sinceDec. 5, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAW212
Plasmid#113234PurposeE. coli expression vector for untagged E. coli Cas1 + Cas2 with CRISPR array, leader GGTAG->CCATCDepositorInsertsCas1
Cas2
Leader-repeat
UseTagsExpressionBacterialMutationGGTAG->CCATCPromoterAvailable sinceAug. 3, 2018AvailabilityAcademic Institutions and Nonprofits only