We narrowed to 10,302 results for: 158

1. pRGEB33

   Plasmid

   #158151

   Purpose

   Construction of inPTG-Cas9 plasmids expressing gRNA within an engineered intron for binary vector plant genome editing.

   Depositor

   Kabin Xie

   Article

   Ding et al Mol Plant. 2018 Apr 2;11(4):542-552. doi

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   Jan. 4, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCAG-TET3G-T2A-Puro

   Plasmid

   #158067

   Purpose

   Encodes the Tet3G transactivator and puromycin resistance.

   Depositor

   Adolfo Rivero-Muller

   Article

   Michalec-Wawiorka et al Int J Mol Sci. 2021 May 14;22(10). pii:

   Insert

   pCAG-TET3G-T2A-Puro
   

   Use

   AAV

   Tags

   puromycin reistance gene

   Expression

   Mammalian

   Mutation

   Promoter

   pCAG

   Available since

   Nov. 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCMV-mGold-Calnexin-N-14

   Plasmid

   #158004

   Purpose

   Visualization of ER in mammalian cells using mGold

   Depositor

   Francois St-Pierre

   Article

   Lee et al Sci Adv. 2020 Oct 23;6(43). pii: 6/43/ea

   Insert

   Calnexin (CANX Human)
   

   Use

   Tags

   mGold

   Expression

   Mammalian

   Mutation

   I474N

   Promoter

   CMV

   Available since

   Oct. 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCTCON2-GFP

   Plasmid

   #158130

   Purpose

   Single fluorescent protein reporter (GFP) for ncAA incorporation (wild-type construct)

   Depositor

   James Van Deventer

   Article

   Potts et al Mol Syst Des Eng. 2020 Feb 1;5(2):573-58

   Insert

   sfGFP
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   GAL1-10

   Available since

   Jan. 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pEMS2232

   Plasmid

   #158401

   Purpose

   High Copy number plasmid containing Ple316 (NR2E1 MiniPromoter) insert

   Depositor

   Elizabeth Simpson

   Article

   Korecki et al Gene Ther. 2021 Feb 2. pii: 10.1038/s414

   Insert

   Ple316 (NR2E1 Human)
   

   Use

   Unspecified

   Tags

   Expression

   Mutation

   Promoter

   Ple316

   Available since

   Feb. 4, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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6. pNHEJ-Cas12a

   Plasmid

   #158713

   Purpose

   CRISPR-assisted-NHEJ system used for genome editing in Mycobacterium smegmatis, expresses FnCpf1, NHEJ machinery of M. marinum

   Depositor

   Yi-Cheng Sun

   Article

   Yan et al mBio. 2020 Jan 28;11(1). pii: mBio.02364

   Insert

   the MmNHEJ machinery (MMAR_4573, MMAR_4574, and MMAR_4575)
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Jan. 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pYPQ132-tRNA2.0

   Plasmid

   #158394

   Purpose

   Golden Gate entry vector to express the 2nd gRNA with tRNA2.0 scaffold (with four MS2 binding sites) without promoter

   Depositor

   Yiping Qi

   Article

   Pan et al Nat Plants. 2021 Jul;7(7):942-953. doi:

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Without promoter

   Available since

   June 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. p663-UBC-V5-GSK3B_IDG-K

   Plasmid

   #158166

   Purpose

   Gateway destination clone of V5-GSK3B (human) tagged with N-terminal V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics

   Depositor

   Ben Major

   Insert

   V5-GSK3B (GSK3B Human)
   

   Use

   Lentiviral; Gateway destination

   Tags

   V5

   Expression

   Mammalian

   Mutation

   Promoter

   Ubiquitin

   Available since

   Aug. 31, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pRK7-Rheb

   Plasmid

   #15888

   Depositor

   John Blenis

   Article

   Tee et al Curr Biol. 2003 Aug 5. 13(15):1259-68.

   Insert

   Rheb (RHEB Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 30, 2007

   Availability

   Academic Institutions and Nonprofits only

10. pDONR221-TMPRSS11D

    Plasmid

    #158447

    Purpose

    Gateway Cloning compatible entry vector for the human TMPRSS11D gene.

    Depositor

    Alejandro Chavez, Sho Iketani

    Insert

    TMPRSS11D (TMPRSS11D Human)
    

    Use

    Gateway entry vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLEX307-DPP4-blast

    Plasmid

    #158452

    Purpose

    Constitutive lentiviral expression of human DPP4.

    Depositor

    Alejandro Chavez, Sho Iketani

    Insert

    DPP4 (DPP4 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA3.1-mas-GRK2RH-Nluc

    Plasmid

    #158605

    Purpose

    Plasmid expressing GRK2(RH)-Nluc in mammalian cells

    Depositor

    Mikel Garcia-Marcos

    Article

    Maziarz et al Cell. 2020 Jun 27. pii: S0092-8674(20)30

    Insert

    myristic acid attachment peptide (mas; MGSSKSKTSNS) - linker (GIKLGG) - GRK2 RH domain - linker (GGTGGS) - Nluc
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 29, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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13. PB-CAG::Cytbow

    Plasmid

    #158995

    Purpose

    Multicolor cytoplasmic cell labeling with a genome-integrating piggyBac transposon vector (randomly expressing tdTomato, mTurquoise2 or mEYFP upon Cre recombination)

    Depositor

    Jean Livet

    Article

    Loulier et al Neuron. 2014 Feb 5;81(3):505-20. doi: 10

    Insert

    Cytbow
    

    Use

    Tags

    The default expressed EBFP2 is fused to H2B

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Oct. 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. p32Lb

    Plasmid

    #158154

    Purpose

    Construct conventional Cpf1 vectors that uses U3p and UBI10p to express single crRNA (or PTC/CA) and FnCpf1, respectively. For plant genome editing.

    Depositor

    Kabin Xie

    Article

    Ding et al Mol Plant. 2018 Apr 2;11(4):542-552. doi

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Jan. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAAV-Syn-SomaGCaMP6f2

    Plasmid

    #158756

    Purpose

    Cell body-targeted GCaMP6f, under synapsin promoter: GCaMP6f followed by a linker, and the EE-RR coiled-coil motif (GCaMP6f-27-EE-RR). As bright as conventional GCaMP6f.

    Depositor

    Edward Boyden

    Article

    Shemesh et al Neuron. 2020 Jun 26. pii: S0896-6273(20)

    Insert

    SomaGCaMP6f2
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    synapsin promoter

    Available since

    Sept. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pPP381

    Plasmid

    #158798

    Purpose

    pRSF-Duet1 derived plasmid containing C-terminally hexa-histidine tagged dCasΦ-2(D394A) in MCS1 for expression in E. coli and protein purification

    Depositor

    Jennifer Doudna

    Article

    Pausch et al Science. 2020 Jul 17;369(6501):333-337.

    Insert

    dCasPhi-2
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    RuvC-inactivating mutation D394A

    Promoter

    Available since

    Aug. 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pYC2085

    Plasmid

    #158721

    Purpose

    CRISPR system used for genome editing in Mycobacterium tuberculosis. Helper plasmid expresses Sth1 Cas9 and the cognate sgRNA, using hygromycin as a selection marker.

    Depositor

    Yi-Cheng Sun

    Article

    Yan et al mBio. 2020 Jan 28;11(1). pii: mBio.02364

    Insert

    Sth1 sgRNA scaffold
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pYPQ-dpcoCas9-SunTag

    Plasmid

    #158410

    Purpose

    CRISPR-dpcoCas9 fused with SunTag recruiting VP64 activators for transcriptional activation

    Depositor

    Yiping Qi

    Article

    Pan et al Nat Plants. 2021 Jul;7(7):942-953. doi:

    Insert

    dpcoCas9-SunTag-rbcS-E9t-ter-ZmUbi-scFv-sfGFP-VP64-GB1-NOS-ter
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    June 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCD5-H3CH13-CO-GCN4-TEV-sfGFP-TS

    Plasmid

    #158740

    Purpose

    Expresses influenza A virus trimeric hemagglutinin A/H3/CH/13 fused to a sfGFP

    Depositor

    Robert de Vries

    Article

    van der Woude et al Protein Sci. 2020 Jul 25. doi: 10.1002/p

    Insert

    Hemagglutinin A/MI/45/15 H1N1
    

    Use

    Tags

    GCN4-TEV-sfGFP-TwinStrep

    Expression

    Mammalian

    Mutation

    AA61-469

    Promoter

    CMV

    Available since

    Nov. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCR-Hyg

    Plasmid

    #158708

    Purpose

    For cloning and constitutive expression of crRNAs in mycobacterium

    Depositor

    Yi-Cheng Sun

    Article

    Yan et al Appl Environ Microbiol. 2017 Aug 17;83(1

    Insert

    crRNA
    

    Use

    CRISPR and Synthetic Biology; Mycobacteria expres…

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Hsp60

    Available since

    Jan. 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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