We narrowed to 305 results for: IPTG

1. pYC01

   Plasmid

   #224586

   Purpose

   IPTG-inducible phage φX174 lysis gene E

   Depositor

   Ophelia Venturelli

   Article

   Cheng et al Mol Syst Biol. 2023 Mar 9;19(3):e11406.

   Insert

   phage φX174 lysis gene E (phiX174p08 )
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   PA1lacO-1

   Available since

   Sept. 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pNH-TrxT

   Plasmid

   #26106

   Purpose

   SGC Empty backbone for bacterial expression

   Depositor

   Opher Gileadi

   Article

   Savitsky et al J Struct Biol. 2010 Oct;172(1):3-13. doi

   Type

   Empty backbone

   Use

   Tags

   6xHis, TEV cleavage site, and TrxA

   Expression

   Bacterial

   Mutation

   Promoter

   T7-lacO (lactose/IPTG inducible)

   Available since

   Dec. 2, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET-28a(+)::NL

   Plasmid

   #141289

   Purpose

   NLUC in pET-28 a (+) backbone for bacterial IPTG inducible expression (KmR)

   Depositor

   Andrew Millar

   Article

   Urquiza-Garcia et al Plant Methods. 2019 Jul 8;15:68. doi: 10

   Insert

   NLUC
   

   Use

   Luciferase

   Tags

   Expression

   Bacterial

   Mutation

   Engineered for high stability (t1/2 = 11.5 days a…

   Promoter

   promoter for bacteriophage T7 RNA polymerase

   Available since

   Feb. 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pTD-NTwinStrep_Sm

   Plasmid

   #45937

   Purpose

   use to create N-terminal Twin-Strep-tag fusion proteins or dual tagged fusion with N-terminal Twin-Strep-tag and C-terminal 6x His-tag

   Depositor

   Thorben Dammeyer

   Article

   Dammeyer et al Microb Cell Fact. 2013 May 20;12(1):49.

   Type

   Empty backbone

   Use

   Tags

   6xHis and Twin-Strep

   Expression

   Bacterial

   Mutation

   Promoter

   Trc (lactose/IPTG inducible)

   Available since

   Sept. 18, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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5. pTD-NTwinStrep_Km

   Plasmid

   #45938

   Purpose

   use to create N-terminal Twin-Strep-tag fusion proteins or dual tagged fusion with N-terminal Twin-Strep-tag and C-terminal 6x His-tag

   Depositor

   Thorben Dammeyer

   Article

   Dammeyer et al Microb Cell Fact. 2013 May 20;12(1):49.

   Type

   Empty backbone

   Use

   Tags

   6xHis and Twin-Strep

   Expression

   Bacterial

   Mutation

   Promoter

   Trc (lactose/IPTG inducible)

   Available since

   Sept. 18, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET28a(+)::NL3F10H

   Plasmid

   #141290

   Purpose

   NLUC:3xFlag:10xHis in pET-28 a (+) backbone for bacterial IPTG inducible expression (KmR)

   Depositor

   Andrew Millar

   Article

   Urquiza-Garcia et al Plant Methods. 2019 Jul 8;15:68. doi: 10

   Insert

   NLUC3F10H
   

   Use

   Luciferase

   Tags

   Flag (x3) and His (x10)

   Expression

   Bacterial

   Mutation

   Engineered for high stability (t1/2 = 11.5 days a…

   Promoter

   promoter for bacteriophage T7 RNA polymerase

   Available since

   Feb. 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pET21a - NR

   Plasmid

   #89625

   Purpose

   Expresses recombinant 6HIS tagged T. cruzi nitrate reductase in E. coli upon IPTG induction

   Depositor

   Judy Lieberman

   Article

   Dotiwala et al Nat Med. 2016 Feb;22(2):210-6. doi: 10.1

   Insert

   Nitrate reductase
   

   Use

   Tags

   6HIS and T7 tag (gene 10 leader)

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   May 22, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pMGA-ptac-sfGFP

   Plasmid

   #139934

   Purpose

   IPTG-inducible sfGFP expression on M. magneticum/E. coli shuttle vector

   Depositor

   Christopher Voigt

   Article

   Furubayashi et al Adv. Funct. Mater. 2021, 31, 2004813

   Insert

   LacI-Ptac-sfGFP
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Aug. 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pFW2100

   Plasmid

   #224212

   Purpose

   Bacteroides genomic editing tool; IPTG inducible-Fncpf1

   Depositor

   Ophelia Venturelli

   Article

   Feng et al Cell Host Microbe. 2022 Feb 9;30(2):200-

   Insert

   Fncpf1
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pET11a-Z-NGFP

    Plasmid

    #52734

    Purpose

    IPTG-inducible expression of Z-NGFP positive control for in vivo split GFP assembly assay

    Depositor

    Lynne Regan

    Article

    Magliery et al J Am Chem Soc. 2005 Jan 12;127(1):146-57

    Insert

    NGFP+Z-fusion
    

    Use

    Tags

    His tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    June 20, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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11. DT044A

    Plasmid

    #159405

    Purpose

    Inducible Perfringolysin O (PFO) expression and purification, controlled under hybrid IPTG-regulated T7/LacO promoter (pRT30)

    Depositor

    Sheref Mansy

    Article

    Toparlak et al Sci Adv. 2020 Sep 18;6(38). pii: 6/38/ea

    Insert

    pr.T7-LacO-6xHis-PFO (Pfo, Clostridium perfringens)
    

    Use

    Synthetic Biology

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    T7/LacO promoter

    Available since

    Feb. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pETM11-6xHis-TEV-hORF1p_K3AK4A

    Plasmid

    #202585

    Purpose

    Allows for IPTG-inducible expression of the human L1RP ORF1 protein with the K3A and K4A mutations in bacteria with an N-terminal 6xHis tag and TEV cleavage site for purification with a minimal scar

    Depositor

    Liam Holt

    Article

    Sil et al Elife. 2023 Apr 28;12:e82991. doi: 10.75

    Insert

    ORF1p
    

    Use

    Tags

    6x His and TEV protease cleavage site

    Expression

    Bacterial

    Mutation

    Changed ORF1p Lysine 3 to Alanine, changed ORF1p …

    Promoter

    Available since

    Nov. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pKPY514

    Plasmid

    #62598

    Purpose

    Encodes Thr251Gly-EcPheRS Under IPTG-Inducible (PT5) Control

    Depositor

    David Tirrell

    Article

    Yuet et al Proc Natl Acad Sci U S A. 2015 Feb 17. p

    Insert

    Thr251Gly-EcPheRS
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Thr251Gly

    Promoter

    T5

    Available since

    March 12, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pET21a - GR

    Plasmid

    #89614

    Purpose

    Expresses recombinant 6HIS tagged T. cruzi Glutathione reductase protein in E. coli upon IPTG induction

    Depositor

    Judy Lieberman

    Article

    Dotiwala et al Nat Med. 2016 Feb;22(2):210-6. doi: 10.1

    Insert

    Glutaredoxin
    

    Use

    Tags

    6HIS

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 22, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pETM11-6xHis-TEV-hORF1p_StammerAAA

    Plasmid

    #202587

    Purpose

    Allows for IPTG-inducible expression of the human L1RP ORF1 protein with the M91A, E92A, and L93A mutations in bacteria with an N-terminal 6xHis tag and TEV cleavage site for purification

    Depositor

    Liam Holt

    Article

    Sil et al Elife. 2023 Apr 28;12:e82991. doi: 10.75

    Insert

    ORF1p
    

    Use

    Tags

    6x His and TEV protease cleavage site

    Expression

    Bacterial

    Mutation

    Changed ORF1p Methionine 91 to Alanine, ORF1p Glu…

    Promoter

    Available since

    Nov. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pETM11-6xHis-TEV-hORF1p_StammerAEA

    Plasmid

    #202588

    Purpose

    Allows for IPTG-inducible expression of the human L1RP ORF1 protein with the M91A and L93A mutations in bacteria with an N-terminal 6xHis tag and TEV cleavage site for purification

    Depositor

    Liam Holt

    Article

    Sil et al Elife. 2023 Apr 28;12:e82991. doi: 10.75

    Insert

    ORF1p
    

    Use

    Tags

    6x His and TEV protease cleavage site

    Expression

    Bacterial

    Mutation

    Changed ORF1p Methionine 91 to Alanine, changed O…

    Promoter

    Available since

    Nov. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pT-GFP-rG1

    Plasmid

    #188967

    Purpose

    IPTG inducible GFP with sgRNA

    Depositor

    Seung-Goo Lee

    Article

    Kim et al Nucleic Acids Res. 2022 Dec 9;50(22):131

    Inserts

    GFP
    sgRNA: agtggaaaacaatgcgaccgactagt
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Ptrc

    Available since

    Sept. 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pT-GFP-rG2

    Plasmid

    #188968

    Purpose

    IPTG inducible GFP with sgRNA

    Depositor

    Seung-Goo Lee

    Article

    Kim et al Nucleic Acids Res. 2022 Dec 9;50(22):131

    Inserts

    GFP
    sgRNA: agtccatgtaatcagcgtctactagt
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Ptrc

    Available since

    Sept. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pET21a - TryX

    Plasmid

    #89623

    Purpose

    [Edit] Expresses recombinant 6HIS tagged T. cruzi tryparedoxin protein in E. coli upon IPTG induction

    Depositor

    Judy Lieberman

    Article

    Dotiwala et al Nat Med. 2016 Feb;22(2):210-6. doi: 10.1

    Insert

    Tryparedoxin
    

    Use

    Tags

    6HIS and T7 tag (gene 10 leader)

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 22, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCA24N-ligase

    Plasmid

    #87741

    Purpose

    IPTG-inducible expression of T4 DNA ligase for protein purification

    Depositor

    Wayne Patrick

    Article

    Wilson et al Protein Eng Des Sel. 2013 Jul;26(7):471-

    Insert

    T4 DNA ligase (30 )
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    T5-lac

    Available since

    March 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge