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pCS2-F3-GCA

Plasmid

#30754

Depositor

Nathan Lawson, Scot Wolfe

Article

Zhu et al Development. 2011 Oct;138(20):4555-64.

Insert

F3 GCA

Use

Mrna production

Tags

FLAG

Expression

Mutation

Promoter

Available since

Nov. 9, 2011

Availability

Academic Institutions and Nonprofits only
pCS2-F1-AGA

Plasmid

#30755

Depositor

Nathan Lawson, Scot Wolfe

Article

Zhu et al Development. 2011 Oct;138(20):4555-64.

Insert

F1 AGA

Use

Mrna production

Tags

FLAG

Expression

Mutation

Promoter

Available since

Nov. 9, 2011

Availability

Academic Institutions and Nonprofits only
pCS2_F1-GAC

Plasmid

#30694

Depositor

Nathan Lawson, Scot Wolfe

Article

Zhu et al Development. 2011 Oct;138(20):4555-64.

Insert

F1 GAC

Use

Mrna production

Tags

FLAG

Expression

Mutation

Promoter

Available since

Nov. 9, 2011

Availability

Academic Institutions and Nonprofits only
pCS2-F2-GAA

Plasmid

#30696

Depositor

Nathan Lawson, Scot Wolfe

Article

Zhu et al Development. 2011 Oct;138(20):4555-64.

Insert

F2 GAA

Use

Mrna production

Tags

HA tag

Expression

Mutation

Promoter

Available since

Nov. 9, 2011

Availability

Academic Institutions and Nonprofits only
pCS2-F1-GCA

Plasmid

#30697

Depositor

Nathan Lawson, Scot Wolfe

Article

Zhu et al Development. 2011 Oct;138(20):4555-64.

Insert

F1 GCA

Use

Mrna production

Tags

HA tag

Expression

Mutation

Promoter

Available since

Nov. 9, 2011

Availability

Academic Institutions and Nonprofits only
pCS2-F1-GCC

Plasmid

#30698

Depositor

Nathan Lawson, Scot Wolfe

Article

Zhu et al Development. 2011 Oct;138(20):4555-64.

Insert

F1 GCC

Use

Mrna production

Tags

FLAG

Expression

Mutation

Promoter

Available since

Nov. 9, 2011

Availability

Academic Institutions and Nonprofits only
pCS2-F1-GCG

Plasmid

#30699

Depositor

Nathan Lawson, Scot Wolfe

Article

Zhu et al Development. 2011 Oct;138(20):4555-64.

Insert

F1 GCG

Use

Mrna production

Tags

HA tag

Expression

Mutation

Promoter

Available since

Nov. 9, 2011

Availability

Academic Institutions and Nonprofits only
pCS2-F1-GCT

Plasmid

#30700

Depositor

Nathan Lawson, Scot Wolfe

Article

Zhu et al Development. 2011 Oct;138(20):4555-64.

Insert

F1 GCT

Use

Mrna production

Tags

HA

Expression

Mutation

Promoter

Available since

Nov. 9, 2011

Availability

Academic Institutions and Nonprofits only
pCS2+MLS-HyPer7

Plasmid

#136470

Purpose

Mammalian expression of mitochondrial matrix targeted ultrasensitive hydrogen peroxide indicator HyPer7 for optical imaging

Depositor

Vsevolod Belousov

Article

Pak et al Cell Metab. 2020 Mar 3;31(3):642-653.e6.

Insert

HyPer7

Use

Tags

Tandem mitochondrial targeting signal of cytochro…

Expression

Mammalian

Mutation

Promoter

CMV, SP6

Available since

Feb. 11, 2020

Availability

Academic Institutions and Nonprofits only

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pCS2+HyPer7-NES

Plasmid

#136467

Purpose

Mammalian expression of cytoplasm targeted ultrasensitive hydrogen peroxide indicator HyPer7 for optical imaging

Depositor

Vsevolod Belousov

Article

Pak et al Cell Metab. 2020 Mar 3;31(3):642-653.e6.

Insert

HyPer7

Use

Tags

Nuclear export signal from the HIV Rev protein

Expression

Mammalian

Mutation

Promoter

CMV, SP6

Available since

Feb. 11, 2020

Availability

Academic Institutions and Nonprofits only

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pCS2+-FLAG3-drAgo2DY-EF

Plasmid

#221716

Purpose

Expression of zebrafish Ago2 with D651E and Y680F mutations and N-terminal 3xFLAG tag in zebrafish embryos.

Depositor

David Bartel

Article

Chen et al Mol Cell. 2017 Dec 21;68(6):1095-1107.e5

Insert

drAgo2DY-EF (ago2 Zebrafish)

Use

Fish expression

Tags

3xFLAG

Expression

Mutation

D651E and Y680F in active site

Promoter

simian CMV IE94

Available since

Oct. 15, 2024

Availability

Academic Institutions and Nonprofits only

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pCS2+-FLAG3-drAgo2

Plasmid

#221712

Purpose

Expression of zebrafish Ago2 with N-terminal 3xFLAG tag in zebrafish embryos.

Depositor

David Bartel

Article

Chen et al Mol Cell. 2017 Dec 21;68(6):1095-1107.e5

Insert

drAgo2 (ago2 Zebrafish)

Use

Fish expression

Tags

3xFLAG

Expression

Mutation

Promoter

Available since

Oct. 15, 2024

Availability

Academic Institutions and Nonprofits only

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pCS2 LRP6-eGFP

Plasmid

#180143

Purpose

to make movies of LRP6 vesicles

Depositor

Edward De Robertis

Article

Colozza et al Sci Rep. 2020 Dec 9;10(1):21555. doi: 10

Insert

Human LRP6 (LRP6 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

SP6

Available since

March 31, 2022

Availability

Academic Institutions and Nonprofits only

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pCS2FA CO Tol2 TPase

Plasmid

#133032

Purpose

Expression vector for production of codon optimized Tol2 transposase

Depositor

Charles Nathan Hancock

Article

Mackey et al MicroPubl Biol. 2020 Jun 11;2020:10.1791

Insert

Codon optimized Tol2 Transposase

Use

In vitro expression

Tags

Expression

Mutation

Codon Optimized

Promoter

Available since

June 15, 2020

Availability

Academic Institutions and Nonprofits only

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pCS2+HyPer7-NLS

Plasmid

#136468

Purpose

Mammalian expression of nucleus targeted ultrasensitive hydrogen peroxide indicator HyPer7 for optical imaging

Depositor

Vsevolod Belousov

Article

Pak et al Cell Metab. 2020 Mar 3;31(3):642-653.e6.

Insert

HyPer7

Use

Tags

Triple nuclear localization signal of SV40 (simia…

Expression

Mammalian

Mutation

Promoter

CMV, SP6

Available since

Aug. 18, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pCS2-XTbx5-EnR

Plasmid

#117249

Purpose

mRNA synthesis

Depositor

Gerald Thomsen

Article

Horb et al Development. 1999 Apr;126(8):1739-51.

Insert

Tbx5-EnR (tbx5-b Frog)

Use

Xenopus expression

Tags

Expression

Mutation

chimeric protein with engrailed repressor domain…

Promoter

Available since

July 9, 2019

Availability

Academic Institutions and Nonprofits only

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pCS2+IMS-HyPer7

Plasmid

#136469

Purpose

Mammalian expression of mitochondrial intermembrane space targeted ultrasensitive hydrogen peroxide indicator HyPer7 for optical imaging

Depositor

Vsevolod Belousov

Article

Pak et al Cell Metab. 2020 Mar 3;31(3):642-653.e6.

Insert

HyPer7

Use

Tags

SMAC/DIABLO

Expression

Mammalian

Mutation

Promoter

CMV, SP6

Available since

Feb. 11, 2020

Availability

Academic Institutions and Nonprofits only

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pCS2FA-H2B-mScarlet3

Plasmid

#224436

Purpose

Construct for labelling cell nuclei in mammalian cells using Histon protein 2B fused to mScarlet3

Depositor

Dorus Gadella

Article

Gadella TWJ et al Nat Methods. 2023 Apr;20(4):541-545. doi

Insert

H2B-mScarlet3

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV & SP6

Available since

Sept. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pCS2+ Grx1-roCherry

Plasmid

#141068

Purpose

Fluorescent biosensor for 2GSH/GSSG ratio

Depositor

Dmitry Bilan

Article

Shokhina et al Redox Biol. 2019 Feb;21:101071. doi: 10.

Insert

Grx1-roCherry

Use

Tags

Expression

Mammalian

Mutation

Changed Alanine 150 to Cysteine, inserted Threon…

Promoter

CMV, SP6

Available since

Feb. 10, 2021

Availability

Academic Institutions and Nonprofits only

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pCS2+8 Flag2AUF1(p45)

Plasmid

#135947

Purpose

Expresses AUF1p45 in mammalian cells. Can also be used to generate mRNA for zebrafish expression.

Depositor

Yimon Aye

Article

Poganik et al FASEB J. 2019 Nov 1:fj201901930R. doi: 1

Insert

heterogeneous nuclear ribonucleoprotein D (HNRNPD Human)

Use

Tags

Flag2

Expression

Mammalian

Mutation

Promoter

CMV and SP6

Available since

Jan. 13, 2020

Availability

Academic Institutions and Nonprofits only

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